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《果树学报》2017,(5)
【目的】对山核桃属种间嫁接亲和性进行探讨分析,为嫁接砧穗的选择提供理论依据。【方法】以山核桃、薄壳山核桃、湖南山核桃2 a(年)生实生苗为砧木,分别与山核桃、薄壳山核桃、湖南山核桃当年生优良穗条嫁接,形成9个砧穗组合,通过萌芽率、成活率、生长指标、光响应曲线和荧光参数等指标综合评价嫁接亲和性。【结果】(1)以湖南山核桃和薄壳山核桃为砧木时,接穗萌芽率最高,分别为80.26%和78.52%,以湖南山核桃为砧木嫁接山核桃萌芽率提高了12%。(2)从嫁接苗的生长量来看,薄壳山核桃不宜嫁接在山核桃和湖南山核桃砧木上;而当以薄壳山核桃为砧木时,能促进山核桃和湖南山核桃的生长;湖南山核桃为砧木,能促进山核桃接穗粗度增加。(3)从光合指标来看,以薄壳山核桃为砧木的Amax显著高于以山核桃和湖南山核桃为砧木的苗木。LSP以薄壳山核桃为砧木时最高,从一定程度上可以表明在相同光照条件下,LSP较高的叶片不易光抑制,湖南山核桃嫁接在山核桃上能增强叶片的LSP,增强其抗光抑制能力。(4)薄壳山核桃为砧木、薄壳山核桃为接穗的嫁接苗栅栏组织最厚(145.665μm),植物利用光能的效率最高,也相应的使山核桃和湖南山核桃接穗的嫁接苗栅栏组织变厚。【结论】山核桃和湖南山核桃亲和性良好,以薄壳山核桃为砧木分别嫁接山核桃和湖南山核桃亲和性好,但分别以山核桃和湖南山核桃为砧木嫁接薄壳山核桃的亲和性差。 相似文献
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<正>薄壳山核桃,又称美国山核桃、长山核桃。安徽省自2010年开展薄壳山核桃科研项目攻关以来,逐步完善了薄壳山核桃在苗木繁育、规模造林、果园化丰产栽培等方面的技术环节,并制订了薄壳山核桃育苗技术操作规程。现将其丰产栽培技术介绍如下。1建园1.1苗木定植春秋季都可定植,但以秋季定植效果为好。 相似文献
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为了解决薄壳山核桃育苗前期生长缓慢、嫁接成活率低、移栽难以成活等突出问题,连续4年进行了育苗方法的探索与嫁接技术的研究,总结出了一套薄壳山核桃大棚高效育苗技术。以大棚代替露天大田培育,促进了苗木前期生长,大大提高了苗木生长量;容器育苗代替大田常规育苗,使苗木根量大大增加,移栽成活率几近100%;大方块芽接代替传统的嫁接方法,使嫁接成活率达到96%以上;8月中下旬嫁接,接后不剪砧,翌年春季定植后再剪砧、除绑。 相似文献
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龙眼实生苗移植当年嫁接育苗技术 总被引：1，自引：0，他引：1
龙眼实生苗移植当年嫁接育苗技术李进华阎鼎羽廖建明（广西林科院经济林果研究所桂林５３０００１）龙眼实生苗移植当年进行嫁接，与传统的育苗方法相比，可缩短育苗周期１～２年，并且苗木生长均匀，出圃率高。据调查，其平均成活率达９２．５４％。１实生苗的选择和处理... 相似文献
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AIM: To examine the autoantibody against α1-adrenoceptor and its biologic activities during the development of renal hypertension. METHODS: Renal hypertension of rat was achieved by clipped renal artery, the titre of autoantibody to α1-adrenoceptor was detected using ELISA immunoassay. Furthermore, the biological offects of these autoantibodies on cultured cardiomyocytes were also examined. RESULTS: After two weeks of clipping renal arteries, both the frequency of occurrence and the titre of autoantibodies to cardiac α1-adrenergic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 12 weeks. The biological effects of these autoantibodies displayed an "agonistic-like" activities on the beating frequency of cultured neonatal cardiomyocytes. CONCLUSION: Autoantibodies against α1-adrenoceptor may play a role in the elevation of peripheral vascular resistance and in the development of cardiac hypertrophy in rats with renal hypertension. 相似文献
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AIM: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact alterations induced by β-particles irradiation remain to be elucidated. The objective of this study was to investigate the ability and the cellular mechanism of local β-particles emission from 188Re to inhibit vascular smooth muscle cells (SMCs). METHODS: The SMCs in vitro were irradiated by 188Re with single doses of 2.6 Gy-25.8 Gy. The effects of β-particles on SMCs, such as effective irradiate doses, the period of inhibition for SMCs proliferation, the changes of cell proliferation rate and DNA synthesis rate, cell cycle progression and related gene expression, were investigated by cell count, [3H]-TdR incorporation, cell cycle progression analysis, cell viability and immunocytochemistry, respectivecy. RESULTS: β-particles irradiation with dose of 5.2 Gy could inhibit significantly SMCs proliferation. At dose of 20.6 Gy DNA synthesis inhibitory rate was 92%, SMCs proliferation rate was only 3%. Renoval of 188Re did not abolish the inhibitory effects of β-particles on SMCs proliferation. The expression of P53 was up regulation and PCNA was down regulation after irradiation. CONCLUSION: β-particles from 188 Re was significantly effective and permanent in inhibiting SMCs proliferation, and inhibitory effect was in dose-dependet manner ED50was 5 Gy, the best dose to inhibit SMCs proliferation was 20 Gy. β-particles irradiation induced SMCs to occur G0/G1 arrest, damaged the ability of SMCs reproliferation and led to cell clonogenic death. P53 and PCNA had regulatiory effects on SMCs proliferation after β-particles irradiation. 相似文献
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《International Journal of Fruit Science》2013,13(1):139-156
Abstract This review is based partly on complete articles and partly on abstracts. Three of the 60 articles deal with the total uptake of elements in strawberry plant organs in two different strawberry production systems, both considered as optimal concerning amount and balance of elements. The effect on fruit quality may be dramatic if the level of a particular element is outside this range, but there may also be effects initiated by differences within the optimal range of elements. Most articles refer to product oriented quality, but some focus on consumer oriented quality, as discussed by Shewfelt (1999). The discussion here is on a general basis, so one should keep in mind that there are cultivar differences and that specification of nutrition ideally should mirror the needs of a single cultivar, or a group of cultivars with similar requirements. Also, to get a complete understanding of the subject future reviews should embrace a broader access of information including the effect on plant development of individual elements, such as the role of calcium in fruit firmness and its importance in cell wall structure. However, the intention here is to narrow the information to results that suggest a direct connection between nutrient uptake and fruit quality. 相似文献
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AIM:To study the effect of L-Arg on plasma content of endothelin (ET) and the expression of proto-oncogene c-fos mRNA in the left ventricle of rats with renovascular hypertensive hypertrophy. METHODS: The level of c-fos mRNA were measured by in situ hybridization. The ET in plasma were measured by radioimmunoassay. RESULTS:After eight weeks of treatment with L-Arg, the expression of c-fos decreased markedly (P＜0.01). The ET content in plasma also decreased significantly by L-Arg(P＜0.01).CONCLUSION: Plasma ET content and the expression of c-fos in the left ventricle of rats with renovascular hypertensive hypertrophy could be decreased by L-Arg administration. 相似文献
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Fulé Peter Z. Crouse Joseph E. Heinlein Thomas A. Moore Margaret M. Covington W. Wallace Verkamp Greg 《Landscape Ecology》2003,18(5):465-486
Fire regime characteristics of high-elevation forests on the North Rim of the Grand Canyon, Arizona, were reconstructed from fire scar analysis, remote sensing, tree age, and forest structure measurements, a first attempt at detailed reconstruction of the transition from surface to stand-replacing fire patterns in the Southwest. Tree densities and fire-/non-fire-initiated groups were highly mixed over the landscape, so distinct fire-created stands could not be delineated from satellite imagery or the oldest available aerial photos. Surface fires were common from 1700 to 1879 in the 4,400 ha site, especially on S and W aspects. Fire dates frequently coincided with fire dates measured at study sites at lower elevation, suggesting that pre-1880 fire sizes may have been very large. Large fires, those scarring 25% or more of the sample trees, were relatively infrequent, averaging 31 years between burns. Four of the five major regional fire years occurred in the 1700s, followed by a 94-year gap until 1879. Fires typically occurred in significantly dry years (Palmer Drought Stress Index), with severe drought in major regional fire years. Currently the forest is predominantly spruce-fir, mixed conifer, and aspen. In contrast, dendroecological reconstruction of past forest structure showed that the forest in 1880 was very open, corresponding closely with historical (1910) accounts of severe fires leaving partially denuded landscapes. Age structure and species composition were used to classify sampling points into fire-initiated and non-fire-initiated groups. Tree groups on nearly 60% of the plots were fire-initiated; the oldest such groups appeared to have originated after severe fires in 1782 or 1785. In 1880, all fire-initiated groups were less than 100 years old and nearly 25% of the groups were less than 20 years old. Non-fire-initiated groups were significantly older (oldest 262 years in 1880), dominated by ponderosa pine, Douglas-fir, or white fir, and occurred preferentially on S and W slopes. The mixed-severity fire regime, transitioning from lower-elevation surface fires to mixed surface and stand-replacing fire at higher elevations, appeared not to have been stable over the temporal and spatial scales of this study. Information about historical fire regime and forest structure is valuable for managers but the information is probably less specific and stable for high-elevation forests than for low-elevation ponderosa pine forests.This revised version was published online in May 2005 with corrections to the Cover Date. 相似文献
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《International Journal of Fruit Science》2013,13(3):101-120
Abstract Saskatoon berry (Amelanchier alnifolia Nutt., Rosaceae) and blueberry (Vaccinium corymbosum L., Ericaceae) are substantially equivalent in all characteristics that are important to the consumer, including fruit color, shape, size, nutrition, texture, and uses. In addition, both fruits are native to North America and they have practically identical historical uses and known health benefits. Their composition, processing, nutritional value and metabolism, intended uses, and levels of undesirable substances are compared. 相似文献
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The objective of this study was to establish a cryopreservation protocol for hawthorn shoot apices (Crataegus pinnatifida Bge.). Cryopreservation was carried out via encapsulation–dehydration, vitrification, and encapsulation–vitrification on shoot apices excised from in vitro cultures. We began by showing that cold-acclimation enhanced the regrowth of cryopreserved apices from 10.0 to 65.5% in encapsulation–dehydration. We then decided that the encapsulation–dehydration method was an optimal cryopreservation method for hawthorn shoot apices in terms of its high recovery after cryopreservation as well as its ease of use compared with vitrification and encapsulation–vitrification. In encapsulation–dehydration, the protocol leading to optimal regrowth was as follows: after cold-acclimation at 5 °C in the dark for 2 weeks, excised shoot tips were pretreated for 24 h at 25 °C on hormone-free Murashige and Skoog [Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473–497] (MS) basal medium with 0.4 mol/L sucrose, then encapsulated and precultured in liquid MS medium with 0.8 mol/L sucrose for 16 h at 25 °C. Precultured beads were dehydrated for 6 h at 25 °C in the dessicator containing 50 g silica gel to a moisture content of 15.3% (fresh-weight basis) before cryostorage for 1 h. In addition, we examined the effect of adding glycerol to both the alginate beads and loading solution to enhance regrowth after cryopreservation in encapsulation–dehydration. In the present study, it was shown that adding 0.5 mol/L glycerol resulted in high regrowth percentages (82.5–90.0%) in four Crataegus species. 相似文献
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以西双版纳黄瓜和‘华黄5号’黄瓜叶片为试验材料,研究了衰老过程中两种黄瓜叶片叶绿素含量变化及脱镁叶绿素脱镁叶绿酸水解酶基因PPH和脱镁叶绿酸a加氧酶基因PAO的表达。结果表明：随着叶片衰老,叶绿素a和叶绿素b含量显著降低,叶绿素a与叶绿素b的比值在0.5左右,推测叶绿素b的含量可能对黄瓜叶片保绿起到重要作用。荧光定量RT-PCR分析结果显示：PPH和PAO在西双版纳黄瓜叶片生长35 d时表达量最高,分别为5.50和1.86;PPH在‘华黄5号’叶片35 d时表达量最高,为7.14,而PAO在35和45 d表达量都很高,分别为3.90和3.91;PPH和PAO在西双版纳黄瓜叶片中的表达量低于在‘华黄5号’中。 相似文献
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AIM:To observe the effect of simvastatin on the proliferation of vascular smooth muscle cells(VSMCs) induced by serum and growth factor PDGF-BB and the effect of simvastatin on the expression of PTEN,a important regulator of G1/S cell cycle transition. METHODS:The DNA synthesis was determined by [3H]-TdR incorporation, cell cycle was examined with flow cytometry, the protein level of PTEN was measured by Western blot method. RESULTS: (1)Simvastatin inhibited [3H]-TdR incorporation in a dose dependent manner. (2) Flow cytometric DNA analysis revealed that simvastatin induced significantly enhancement of G0/G1 phase and decrease in S phase VSMCs.(3)Simvastatin increased protein level of PTEN and mevalonate, a metabolite of HMG-COA, reversed the effect of simvastatin on PTEN protein expression. CONCLUSION:Simvastatin may inhibit proliferation of VSMCs and retarded cell cycle in G0/G1 phase by increasing PTEN expression through inhibiting synthesis of mevalonate. 相似文献
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A two-year field study investigated the possible effects of grain sorghum (Sorghum bicolor [L.] Moench) and uncultivated areas on the abundance of generalist predators in commercially-managed cotton (Gossypium hirsutum L.) fields in Texas, USA. From 63 to 70 fields were sampled for pests and predators over nine consecutive weeks during early stages of cotton development. Additional data on agronomic practices and landscape composition at three spatial scales were also collected for each field. Stepwise regression analyses were used to determine the relationships of landscape, agronomic and prey variables to the abundance of generalist predators. Because the variables most closely linked to predator levels could vary over time, separate regressions were conducted for three time periods corresponding to stages of grain sorghum growth (half-bloom, hard-dough, maturity) in each year. Significant relationships between predator abundance and agricultural landscape composition appear in both years and in all three time periods, but the specific relationships of landscape variables to cotton predator levels differed between and within years. At maturity in 2001, predator levels rose as the amount of uncultivated land from 1.6 to 3.2 km distant and the perimeter shared with grain sorghum increased. During 2002, the area of grain sorghum (half-bloom) and uncultivated land (hard-dough) within 1.6 km of cotton fields were both positively related to predator numbers. Cotton planting dates and the abundance of cotton fleahoppers (Pseudatomoscelis seriatus [Reuter]) were also strongly linked to predator numbers during both years. Results suggest that the total amount of grain sorghum or uncultivated land in an area is more important than the presence of these habitats adjacent to cotton fields, and that landscape composition may sometimes be the most important factor in determining predator abundance. 相似文献