共查询到19条相似文献，搜索用时 375 毫秒
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目的：建立HPLC测定复方二仙草胶囊中淫羊藿苷含量的方法。方法：Shim-pack VP-ODS C18分析色谱柱（4.6 mm×150 mm,5μm）,Phenomenex ODS C18保护柱（3 mm×4 mm）。流动相：乙腈-水（26∶74）,流速1.0 mL/min,检测波长270 nm。结果：淫羊藿苷保留时间为13.8 min。以峰面积Y对进样量X（μg）线性回归,淫羊藿苷回归方程：y=2 732.7x＋12.860r,=1.000 0,线性范围0.1-1.5μg。淫羊藿苷回收率为102.16%,RSD为1.92%。结论：本方法操作简便,结果准确可靠,可用于复方二仙草胶囊中淫羊藿苷的含量测定。 相似文献
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《畜牧与兽医》2014,(8):21-24
对健康雌性小鼠灌服不同剂量淫羊藿多糖,研究淫羊藿多糖对卵泡发育及卵母细胞体外成熟的影响。结果表明:连续灌服7 d淫羊藿多糖后,高剂量组(6 mg/0.2 mL),中剂量组(4 mg/0.2 mL)与对照组子宫卵巢系数分别为0.403%,0.245%和0.146%,差异显著(P<0.05)。灌服淫羊藿多糖后小鼠卵巢卵泡个数和直径均有所提高。卵巢组织学分析表明,随着给药剂量的增加,有腔卵泡的数量也有增加,中、高剂量组卵巢上出现黄体。卵母细胞体外成熟培养表明,中、高剂量组所获GVBD卵母细胞百分率(61.4%和73.0%)与对照组相比(50.0%)均有显著提高(P<0.05)。本研究表明,小鼠每天灌服淫羊藿多糖46 mg,连续1周后可明显促进卵泡发育,并提高卵母细胞体外成熟率。 相似文献
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藿芪灌注液中淫羊藿苷和黄芪甲苷稳定性试验研究 总被引：1，自引：0，他引：1
为了研究藿芪灌注液的稳定性,采用HPLC法测定藿芪灌注液中淫羊藿苷与黄芪甲苷含量,使用Wonda Sil C18柱(4.6 mm×250 mm,5μm),流动相:乙腈-水28∶72,柱温40℃,检测波长270 nm,进样量10μL,流速1.0 m L/min测定淫羊藿苷的含量;使用ZORBAX SB-C18柱(4.6 mm×150 mm,5μm),漂移管温度为90℃,载气流速为2.5 L/min,流动相:甲醇-水65∶35,柱温:40℃,流速:1 m L/min,用蒸发光散射检测器检测测定黄芪甲苷的含量。藿芪灌注液于高温60℃放置10 d,高湿度90%±5%放置10 d,经强光照射4500±500LX放置10 d,加速试验在温度40℃±2℃、相对湿度75%±5%的条件下放置6个月,长期试验在温度25℃±2℃、相对湿度60%±10%的条件下放置12个月,考察藿芪灌注液在不同条件存放后的性状、鉴别、p H、淫羊藿苷含量、黄芪甲苷含量以及无菌检查等项目的变化。结果表明,藿芪灌注液性质稳定,在不同条件存放后所有指标均未有明显变化,符合质量标准要求。 相似文献
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为全面质量评价饲料添加剂淫羊藿提取物，用高效液相色谱（HPLC）方法构建不同品种及地区淫羊藿提取物的特征图谱，并测定其中5个质量控制成分的含量。以1 mL/min的流速，乙腈-水的流动相，35℃的柱温，270 nm的检测波长，20μL的进样量为条件，用Waters C18(4.6 mm×250 mm,5μm)的柱子上机。建立18批淫羊藿提取物的特征图谱，用外标法测定其中5个成分含量。建立的淫羊藿提取物特征图谱定有7个共有特征峰，基于高分辨数据分析和标准品比对，指认5个峰。采用聚类分析及主成分分析（PCA），分析了不同淫羊藿提取物的关联性。测定10批市场销售的淫羊藿提取物产品中五种黄酮醇苷的含量。建立的淫羊藿提取物特征图谱及含量测定方法符合方法学要求，为市售淫羊藿提取物的质量控制管理和资源开发提供了理论依据。 相似文献
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The assay was aimed to optimize the extraction process of the compound Epimedium by orthogonal experimental design. The extraction process of the compound Epimedium was optimized with water volume, extraction times and extraction time as independent variables. The extraction ratio of icariin was choosed as an evaluation index. The optimized extraction conditions were as follows, extracted in 10 times water (solid-liquid rate at 1∶10) for 3 times and heated for 1.5 h per time. These results indicated that the optimum extraction technology by the orthogonal experimental design provided experimental bases for improving the content of icariin and its industrial production. 相似文献
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建立了德拉昔布咀嚼片溶出度测定的高效液相色谱法,并对德拉昔布咀嚼片和Deramaxx咀嚼片的溶出度进行比较。方法为浆法,转速为50 r/min,0.8%十二烷基硫酸钠为溶出介质,于5、10、15、20、30、40、50 min分别采样;采用Waters XSelect H88 C18(4.6 mm×250 mm,5μm)色谱柱,磷酸盐缓冲液(pH4.5)-乙腈(52∶48,V/V)为流动相,检测波长252 nm;柱温30℃;外标法定量。结果表明,德拉昔布在5~25μg/mL浓度范围内线性良好(r2=0.9992),检测限与定量限分别为0.81μg/mL、2.57μg/mL;平均回收率为101.8%±0.91%;德拉昔布咀嚼片与Deramaxx溶出度在30 min内分别为87.78%±1.91%和88.76%±2.05%,符合规定。试验所建立的方法简便、准确、专属性强,可用于德拉昔布咀嚼片的溶出度测定。 相似文献
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Barleys, a major source of feed, are all coarsely dry‐rolled before feeding beef and dairy cattle in North America. The shapes of coarsely dry‐rolled barley particles are not round but very irregular. The model for geometric mean is not suitable for determining irregular barley particle size. In a previous study, we tested four different published models to determine the mean and median particle sizes of coarsely dry‐rolled barley with irregular particle size shapes and found that the Pond’s model with 0 mm = 100% was the best model to compute mean and median particle sizes of the coarsely dry‐rolled barley samples expressed as per cent cumulative weight oversize. However, we still did not know which parameter, median or mean particle size, was the best indicator to predict rumen nutrient degradation and availability. This information is badly needed. The objective of this study was to study the relationship between the mean and median irregular particle sizes and nutrient availability of various barley varieties which were coarsely dry‐rolled. The results revealed a stronger correlation between the median irregular particle size (not mean particle size) and the rate (Kd) and extent (ED) of rumen degradation of dry matter (Kd: R = ?0.78, p = 0.065; EDDM: R = ?0.89, p = 0.016), starch (Kd: R = ?0.96, p = 0.003; EDST: R = ?0.95, p = 0.003) and crude protein (Kd: R = ?0.59, p = 0.215; EDCP: R = ?0.89, p = 0.019). In conclusion, it is the median but not mean particle size that has stronger correlation with rumen degradation kinetics. The 79.9%, 78.3% and 91.0% of variation of effective degradability of dry matter, crude protein and starch, respectively, could be explained by the median irregular particle size. 相似文献
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The aim of this study was to develop sustained release plant extracts as a potential alternative to antibiotic growth promoters for growing pigs. Pellets with a core based on microcrystalline cellulose and 3 active compounds (eugenol, carvacrol, and thymol) were prepared using rotary fluidized-bed technology. Two particle sizes were produced that had a mean size of approximately 250 and 500 mum. Results show the process was able to produce pellets with a spherical and homogenous form when 10% of the active compounds were incorporated into the core. When active compounds were increased to 20%, the pellet became stickier, and the yield decreased from 90 to 65%. Different amounts of coating in the form of an aqueous-based ethylcellulose (EC) dispersion (Surelease) were applied to the core to modify the release of active compounds. The efficacy of the coating was evaluated in vitro using a flow-through cell apparatus. The time to achieve 50 and 90% dissolution increased with the increase in particle size (P < 0.05) and the increase in EC-coating level from 10 to 20% (wt/wt; P < 0.05), indicating the ability of the process to slow release depending on particle size and the amount of polymer applied. Differences in the release of the active compounds were observed in the same formulation of pellets, except for the formulation with small 10%-EC-coated particles, in which the active compounds were rapidly dissolved (more than 85% in 15 min or less). For all other formulations, the dissolution time for eugenol was always faster than for thymol or carvacrol. The close monitoring of plant extract behavior in the gastrointestinal tract could become a key factor in the continued use of phyto-molecules as alternatives to antibiotic growth promoters and in optimizing the balance between cost and efficacy. Different microencapsulation technologies can be used, of which the rotary fluidized bed warrants consideration because of the quality of the products obtained. 相似文献
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为了制备沙拉沙星的新剂型,评价其体外释放度,采用响应面法优化沙拉沙星/β-环糊精包合物微囊的制备条件,用扫描电子显微镜(SEC)进行表征试验,对环糊精包合物微囊的粒径和释放度进行评价。响应面法优化后的参数为沙拉沙星和β-环糊精混合液以300 r/min转速在50℃下搅拌4 h,粒径试验表明包合物粒径平均值为1.0μm。体外释放度试验表明,SAR/β-CD包合物在60 min后累积溶出度达到97%左右并保持稳定,物理混合物60 min后累积溶出度仅63%左右,SAR粉剂在60 min后累积溶出率仅74.4%左右。将沙拉沙星成功制备成一种新型包合物制剂,提高了药物的释放性质,对沙拉沙星的推广具有积极意义。 相似文献
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盐酸金霉素超微粉制备及其在鸭体内的药代动力学研究 《畜牧与饲料科学》2023,44(1):105-110
[目的] 研究盐酸金霉素超微粉在鸭体内的药代动力学特征。[方法] 采用气流粉碎技术将盐酸金霉素普通原粉制备成800目超微粉,再添加适宜辅料制成含量为20%的盐酸金霉素可溶性粉,通过影响因素试验和加速试验对制备的盐酸金霉素超微粉进行稳定性评价;将30只90日龄绍兴麻鸭随机分为A、B、C 3个试验组,每组10只,分别经口单剂量灌服60目普通原粉制备的盐酸金霉素可溶性粉、800目超微粉制备的盐酸金霉素可溶性粉、80目普通原粉制备的盐酸金霉素可溶性粉,剂量均为15 mg/（kg·BW）（以金霉素计）,比较不同粒径原料制备的盐酸金霉素可溶性粉在鸭体内的药物代谢过程。[结果] 利用800目超微粉制备的盐酸金霉素可溶性粉处方、工艺稳定可行,通过稳定性试验考查,质量可靠;不同粒径原料制备的盐酸金霉素可溶性粉主要药代动力学特征如下：试验B组达峰时间（Tmax）显著（P<0.05）快于A组和C组;试验B组药时曲线下面积（AUC0-t）、达峰浓度（Cmax）均显著（P<0.05）大于A组和C组;试验B组消除半衰期时间（t1/2）显著（P<0.05）长于A组和C组,A组和C组之间无统计学差异（P>0.05）;试验B组体内存留时间（MRT）显著（P<0.05）长于A组和C组。[结论] 鸭灌服相同剂量的金霉素[15 mg/（kg·BW）],相较于普通原粉制备的盐酸金霉素可溶性粉,盐酸金霉素超微粉在体内1.5 h即达到血药峰浓度,吸收迅速且有效血药浓度维持时间长。研究获得的药动学数据可为盐酸金霉素用于动物临床治疗提供参考。 相似文献
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不同粒径大黄中有效成分溶出度对比 总被引：1，自引：0，他引：1
目的：测定不同粒径大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的溶出量。方法：用高效液相色谱法测定其溶出量。结果：随着粒径变细,其溶出量都在增加,但粉碎到300目以上时,其溶出量反而下降。结论：大黄超微粉的应用以200目效果最好。 相似文献
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试验以美国转基因大豆为原料,研究了粉碎过程中不同水分含量(13.21%、14.82%、16.71%)和筛孔直径(2.02、.53、.0、3.5 mm)对粉碎产品粒度的影响和不同挤压膨化温度(130、140、150℃)、不同粒度和不同水分含量对膨化大豆中脲酶活性和蛋白质溶解度的影响,筛选出加工膨化大豆的最优工艺条件。研究结果表明,大豆粉碎粒度随水分含量和筛孔孔径的增大而增大;大豆最佳挤压膨化加工参数为:水分含量13.21%,筛网孔径3.5mm,膨化温度130℃。在此加工参数条件下,膨化全脂大豆的脲酶活性为0.248 U/g,蛋白质溶解度75.52%。 相似文献
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In order to study the main effects of particle size, three ruminally fistulated cows (550 to 580 kg BW) were fed a constant low concentrate level (3.56 kg DM/d, 20% of total DMI) and a fibre-rich hay (approximately 60% NDF in DM) in long (28.7 mm), chopped (9.2 mm) and fine ground (2.9 mm) form in a 3 x 3 Latin square design. In another three factorial experiment with 8 wethers (4 animals were ruminally fistulated, mean BW = 68 kg) the main effects and interactions of the above mentioned hay particle sizes at two concentrate levels (10.4 to 13.3% and 29.5 to 40.1% of DMI, resp.) and two intake levels (restricted and ad libitum) were investigated. In comparison to long hay (28.7 mm), feeding of chopped hay (9.2 mm) at low concentrate levels, increased not only the hay intake (7% in dairy cows and 13% in sheep) but also the intake of digestible organic matter (12% in dairy cows and 32% in sheep), due to an increase in the apparent digestibility of OM by 3.8% in dairy cows and 8.2% in sheep. Ad libitum feeding of fine ground hay in combination with low concentrate amount in the ration increased the passage rate in the hindgut and consequently the hay intake, but not the intake of DOM, due to a significant depression of digestibility, especially of fibre fractions (4 to 7% in dairy cows and 4.5 to 14% in sheep), in comparison to 28.7 and 9.2 mm hay particle sizes. The digestibility decreased significantly with restricted feeding of fine ground hay in sheep only in comparison to 9.2 mm particle size. A threefold increase of concentrate amount leveled out all effects of the particle size reduction. The effect of particle size was more pronounced in sheep than in dairy cows. 相似文献