共查询到20条相似文献，搜索用时 859 毫秒
1.
卵黄蛋白原在剑尾鱼体内不同组织的分布及雌激素作用对其表达的影响 总被引：1，自引：0，他引：1
为研究卵黄蛋白原(vitellogenin,vg)在剑尾鱼(Xiphophorus helleri)不同组织器官的表达情况,以及了解雌激素对不同组织中Vg表达的诱导作用,将成年雌、雄剑尾鱼及幼鱼暴露于50 μg/L的17β-雌二醇(E2)溶液中20 d,未暴露的剑尾鱼作对照,采用免疫组化法对各处理组剑尾鱼进行Vg的组织定位研究.结果表明,剑尾鱼Vg主要分布于成熟雌鱼的肝脏、脾脏和肠的血管、淋巴管,在正常幼鱼及雄鱼的以上各组织中均不表达.17β-雌二醇暴露下,剑尾鱼雌、雄成鱼和幼鱼的肝脏以及肾、脾、肠等组织的血管及淋巴管均呈Vg阳性.但Vg颗粒只在肝实质细胞中观察到,在其他组织中如脾脏、肠固有结缔组织等,Vg颗粒仅出现于血管,在细胞内未观察到.以上结果表明,剑尾鱼Vg在肝细胞中合成,通过血液循环到达其他组织;雌激素可诱导雌、雄剑尾鱼及幼鱼肝组织中Vg的表达.剑尾鱼幼鱼及雄鱼Vg的非正常表达可应用于环境雌激素效应评价. 相似文献
2.
前期报道了利用尼罗罗非鱼LG22上性别连锁的分子标记检测到养殖群体存在天然XY雌鱼,但其能否用于培育YY超雄罗非鱼尚不清楚。本研究首先引入遗传性别受LG22染色体严格控制的CQ尼罗罗非鱼群体和具有天然XY雌鱼的WC群体,将CQ群体XY雄鱼与WC XY雌鱼杂交,检验杂交F1 YY超雄鱼是否可用于控制后代性别,并比较杂交F1 XY和YY罗非鱼体质量、性腺指数、血清激素水平和性腺基因表达情况。研究发现,CQ XY雄鱼和WC XY雌鱼交配,获得的F1 中具有25%的YY超雄鱼,经鉴定为全雄且可育。将F1 YY超雄鱼与WC XX雌鱼、WC XY雌鱼（母本）、杂交F1 XX雌鱼和CQ XX雌鱼交配,后代几乎全雄,仅在与F1 XX雌鱼交配后代中有2尾雌鱼（雄性率98%）。在孵化后180 d,杂交F1中XY和YY个体的体重、性腺指数、血清激素水平差异不显著。基因表达分析发现,YY鱼精巢中AmhX/AmhY mRNA表达显著高于XY鱼,而Dmrt1 和Cyp11b2 mRNA表达水平差异不显著。杂交F1 YY和XY鱼生理指标无明显差异。因此,采用尼罗罗非鱼天然XY雌鱼能够培育YY超雄鱼,且该YY超雄鱼能够用于罗非鱼性别控制。 相似文献
3.
YY型莫桑比克罗非鱼雌性转化后测交筛选的研究 总被引：6，自引：0，他引：6
采用与ＹＹ型雄鱼测交的方法，从１９７尾转化雌鱼中初步选出１５尾ＹＹ型雄鱼，其中３尾鱼的Ｆ１雄性率为１００％，１０尾鱼的Ｆ１雄性率为９０．６％－９８．４％。对３尾初选ＹＹ型雄鱼进行重复验证，其中２尾鱼的Ｆ１中，ＹＹ型雄性比率分别是４５．８％和４８．１％，Ｘ＾２检验符合５０％ＹＹ型雄鱼的理论比值；另１尾ＹＹ型雌鱼又与ＹＹ型雄鱼交配，Ｆ１中ＹＹ型雄鱼为１００％。３尾母本均再次被证实为ＹＹ型雌鱼。雌性转化 相似文献
4.
5.
于2017年4月底至6月初,进行台湾泥鳅的人工繁殖。亲鱼为天津鸿腾水产科技发展有限公司于2014年春天从我国台湾省引进。共选用台湾泥鳅约2 500kg,其中雌鱼约1 800kg;雄鱼约700kg,雌雄比例约为2.5∶1,雌鱼、雄鱼平均体重分别为121.88g、126.98g。雄亲鱼平均"胸鳍长/体长比"为15.71%,显著大于雌亲鱼的10.08%(P<0.001),并且雌、雄鱼之间数据不重叠。雌鱼背部肌肉注射绒毛膜促性腺激素(HCG)加鲫鱼脑垂体催产,雄鱼不使用催产素。人工授精后,将受精卵均匀地洒在孵化池内的网框上进行孵化,孵化水温18.5~22.5℃,受精约48~52h后破膜孵化。雌鱼每尾产卵量28.7g,平均每克卵数2 061粒,受精率82.98%,平均孵化率97.4%。2017年获得水花约7亿尾。 相似文献
6.
雌核发育彭泽鲫后代理论上应为全雌群体,前期研究发现实验室养殖彭泽鲫F1代(相比池塘养殖)和实验室高密度养殖F2代(1.28尾/L,相比低密度0.64尾/L)组中出现了高比例的雄鱼。之前研究认为Vtg B和ZP2基因是雌性特异性表达的基因,本试验对F1、F2代雌雄鱼Vtg B和ZP2表达及卵黄蛋白原含量差异进行研究,结果发现,实验室养殖F1代雄鱼性腺中Vtg B和ZP2的表达分别极显著和显著高于雌鱼(P0.01,P0.05);池塘养殖F1代雄鱼性腺中Vtg B和ZP2的表达分别极显著高于和低于雌鱼(P0.01);F2代低密度养殖组中雄鱼性腺中ZP2的表达极显著高于雌鱼(P0.01)。实验室养殖F1代雄鱼肝胰脏中Vtg B和ZP2的表达分别极显著高于和低于雌鱼(P0.01);池塘养殖F1代雄鱼肝胰脏Vtg B和ZP2的表达均极显著低于雌鱼(P0.01);F2代雄鱼肝胰脏中Vtg B和ZP2的表达极显著高于雌鱼(P0.01)。F1代实验室养殖雄鱼全鱼卵黄蛋白原含量极显著低于雌鱼(P0.01);F1代池塘养殖雄鱼性腺、肝胰脏中卵黄蛋白原含量分别极显著高于和低于雌鱼(P0.01);F2代雄鱼性腺、肝胰脏中卵黄蛋白原含量极显著高于雌鱼(P0.01)。本研究表明,彭泽鲫Vtg B和ZP2基因并非雌性特异性表达,且不同的养殖方式、密度影响雌雄鱼Vtg B和ZP2表达及卵黄蛋白原含量。 相似文献
7.
亲鱼要血统纯正。但由于尼罗罗非鱼现在纯正的比较少，所以要选择体高、口和头小、可食部份多的鱼。雄鱼一公斤以上(2—3龄)，雌鱼600克以上(2—3龄)。一公斤以上的雄鱼，每尾配雌鱼3—4尾。要生产10万尾鱼苗，就要平均体重800克的雌鱼250尾、平均体重一公斤的雄鱼60—80尾。 相似文献
8.
大黄鱼性别特异SNP标记的开发与验证 总被引：1，自引：1，他引：0
大黄鱼是我国养殖量最大的海水经济鱼类,其雌鱼生长显著快于雄鱼,但两性的外部形态差异不明显,也没有异形性染色体,依靠传统方法无法对其活体准确进行生理性别和遗传性别的判别与鉴定,需要开发性别特异的分子标记。本研究从2尾雌鱼和2尾雄鱼、以及分别由50雌鱼与50尾雄鱼组成的2个混合样品的基因组重测序数据比较中筛选与性别显著关联的SNP位点,对其中11个位点分别设计引物在15尾雌鱼和15尾雄鱼中扩增出PCR产物进行Sanger测序验证,鉴定出1个与性别完全连锁的位点(SNP6,15尾雌鱼均为纯合、15尾雄鱼均为杂合)。然后,设计等位基因特异性PCR引物,其中包括2条雌性与雄性通用引物和1条雄性特异引物,在闽—粤东族与岱衢族大黄鱼合计近2 200个个体中进行扩增,结果在全部雌鱼中都只扩增出1个348 bp的条带,而在全部雄鱼中还扩增出1个194 bp的Y染色体特异条带,检出率达到100%。研究表明,大黄鱼属于XX♀-XY♂类型的性别决定。本研究鉴定出一个雄性特异SNP标记,并建立了一种新的大黄鱼遗传性别鉴定技术,为大黄鱼单性育种、基因组选择育种和性别决定分子机制研究提供了重要的技术手段。 相似文献
9.
《南方水产》2003,(4)
030424一个养殖虹缚种群内存在的生长率、饵料转化率和抗病力的遗传差异=Genetie variation for growth rate,feed con-version effieieney,and disease resistance ex-ists within a farmed POPulation of rain加wtrout〔刊,英」门HenlyonM,JokuTnsenA,Be堪P…// Aquae.一2002,209(1/4)一59一76 将30尾雄鱼与30尾雌鱼用部分正交设计法进行交配。每尾雄鱼与两尾雌鱼交配,每尾雌鱼与两尾雄鱼交配,产生了50个存活的全同胞系(29尾雄性,25尾雌性)。将这些鱼饲养215d,测定第52一第215天期间成活率、体重增长率和饵料转化效率、以及病毒性出… 相似文献
10.
为了解裸体异鳔鳅(鱼它)(Xenophysogobio nudicorpa)人工繁殖和胚胎发育规律，以从金沙江采集到的10尾雌鱼(平均体长17.5 cm,体质量22.3 g)、8尾雄鱼(平均体长15.6 cm,体质量17.5 g)作为亲本，分3批次开展了人工繁殖试验，并观察在水温(20.0±0.5)℃下该鱼的胚胎发育情况。试验结果显示：在水温(15.0±0.5)、(18.0±0.5)、(20.0±0.5)℃的条件下催产，催产剂(雌鱼第1针注射LHRH-A3 2μg/kg,第2针注射LHRH-A3 10μg/kg+HCG 1 200 IU/kg+PG 8 mg/kg,间距12 h;雄鱼不注射)的效应时间分别为21、15、14 h,受精率分别为8.5%、76.8%、74.5%。将受精卵在水温(15.0±0.5)℃条件下孵化288 h,大部分仔鱼未能正常出膜；当水温升至(18.0±0.5)℃,大部分仔鱼在孵化168 h后破膜而出，但仍有小部分无法正常出膜；当水温升至(20.0±0.5)℃时，仔鱼正常出膜，且出膜时间缩短至120 h。裸体异鳔鳅(鱼它... 相似文献
11.
Three distinct forms of vitellogenin (Vg), 600 kDa VgA and VgB and 400 kDa Vg, were discovered biochemically in estrogen treated female plasma. By sequencing of the three Vg cDNAs, the VgA and VgB were recognized as complete Vgs having all yolk protein (YP) domains, and the 400 kDa Vg was thought to be phosvitinless (Pvl) Vg lacking phosvitin (Pv) domain. 相似文献
12.
K. Hiramatsu N. Hiramatsu A. Hara T. Matsubara C.V. Sullivan 《Fish physiology and biochemistry》2003,28(1-4):347-348
Three forms of female-specific plasma protein were purified from blood plasma of estrogen-treated white perch, characterized, and classified as three distinct vitellogenins (VgA, VgB, and VgC). This study describes the first purification of three classes of native Vg from any vertebrate and sets the stage for discovery of the different functions of each type of Vg. 相似文献
13.
Three types of vitellogenins (Vgs) namely vitellogenin A (VgA), vitellogenin B (VgB) and vitellogenin C (VgC) have been identified in fishes. The existence of VgA and VgB is reported in the Indian freshwater murrel Channa punctatus. Gene-specific primers were designed using available nucleotide sequences in National Centre for Biotechnology Information (NCBI), for amplification of VgA and VgB cDNA. Differential processing of Vgs is evident in many fishes. Adult male murrel expressed both the VgA and VgB genes when estradiol-17β (E2) is injected in vivo and Vg levels in blood quantified by Enzyme linked immunosorbent assay (ELISA) showed a dose-related response in such treatments. Cultured hepatocytes on treatment with E2, however, expressed only VgB as detected by RT-PCR, suggesting different regulatory mechanism for the VgA and VgB genes. 相似文献
14.
N. Hiramatsu T. Matsubara A. Hara D.M. Donato K. Hiramatsu N.D. Denslow C.V. Sullivan 《Fish physiology and biochemistry》2002,26(4):355-370
Three forms of female-specific plasma protein (FSPP 1-3) were purified from blood plasma of estrogen-treated white perch (Morone americana) by combining several types of ion-exchange chromatography including a novel, fast flow, strong anion exchanger (POROS media),
followed by gel filtration. Native FSPP 1, FSPP 2 and FSPP 3 had molecular masses of 532 kDa, 532 kDa and 426 kDa, respectively.
The apparent mass of purified FSPP 1 and FSPP 2 after SDS-PAGE under reducing conditions was ∼ 180 kDa, while FSPP 3 appeared
as a major ∼ 148 kDa band. All of the FSPPs resembled one another with respect to amino acid composition but each appeared
to be immunologically distinct. In double immunodiffusion using anti-total FSPP (antiserum raised against vitellogenic female
plasma pre-absorbed by male plasma), each FSPP formed one precipitin line that crossed those produced by both others. A rabbit
antiserum was raised against each FSPP and absorbed with combinations of the other two FSPPs to ensure specificity. Using
the antisera, each FSPP was detected by immuno-electrophoresis in plasma from vitellogenic females or estrogen-treated male
or immature fish, but no FSPP was detected in normal male plasma. Endoprotease (Asp-N) digests of the FSPPs were subjected
to HPLC separation for N-terminal sequencing and mapping of isolated peptides to published vitellogenin (Vg) sequences. Results
of these analyses indicate that white perch FSPP 1, FSPP 2, and FSPP 3 can be classified into three Vg groups identified in
previous studies: VgA, VgB, and VgC-like protein, respectively. This is the first report, of which we are aware, on isolation
of more than two Vg proteins from any species of vertebrate except the chicken.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
15.
The relevance of mitochondrial lineages of Taiwanese cultured grey mullet,Mugil cephalus,to commercial products of Roe 
下载免费PDF全文
下载免费PDF全文 Mugil cephalus is an important aquaculture species in Taiwan with highly valuable roe. In order to obtain its roe, mullet fry from various Taiwan estuaries are raised in aquaculture ponds until maturity. However, not all female mullets have developed ovaries. Therefore, we have attempted to use DNA profiling to aid selection of mullet fry for aquaculture. A large proportion of North coast mullet and some West coast mullet were identified as cytochrome oxidase subunit I (COI) mitochondrial lineage 1. COI mitochondrial lineage 2 was dominant in the west and east coast estuaries, whereas COI mitochondrial lineage 3 was fewest and only was present in Chang‐hua county (middle west of Taiwan). The gonadosomatic index (GSI) of lineage 1 individuals ranged from 0 to 5, no matter where the mullet fry were captured. The GSI of both the west and east coast lineage 2 individuals ranged from 0 to over 15, but the GSI of lineage 2 of the I‐lan (north east of Taiwan) population was generally lower than that of western populations. These findings suggest that a genetic difference whereby west coast lineage 2 mullet yield heavier roe although the body size of lineage 1 individual is larger than that of lineage 2. Thus, lineage 2 individuals with their normal GSI distribution are the most economically viable. The application of the rapid screening of mitochondrial lineages is expected to help aquaculture farmers cultivate lineage 2 fry for roe production rather than lineage 1. 相似文献
16.
Induction of fertilizable eggs by conspecific vitellogenin implantation in captive female walking catfish,Clarias batrachus (Linn.) 下载免费PDF全文
下载免费PDF全文 Debapriya Bhattacharya Shrabanti Sarkar Subir Kumar Juin Panchanan Nath 《Aquaculture Research》2018,49(9):3167-3175
The synthesis of vitellogenin (Vg) is induced by conspecific Vg (Vg1 and Vg2) and estradiol‐17β (E2) as demonstrated by the pattern of 3H‐serine incorporation in the liver and plasma proteins. The incorporation studies indicated that the label was first incorporated into the liver after which it appeared in the blood in both E2‐ and Vg‐treated male catfish. Since Vg was capable of inducing its own synthesis, experiments were conducted in females during preparatory–prespawning period (March–May) to make them gravid by implanting Vg pellets. Two implantations of 4 mg Vg1 pellets into female catfish with an interval of 15 days, followed by laboratory maintenance for 45 days of initial implantation showed a significant increment in ovarian weight with concomitant formation of yolky oocytes through synthesis and incorporation of Vg, whereas Vg2 implantation was not effective in this regard. Histological observation of yolky oocytes in Vg1‐treated group showed the peripheral migration of germinal vesicle (eccentric germinal vesicle), which indicates the onset of maturation. On 45th day, third implantation with 2 mg Vg pellets was performed and after 15 days, fish were hormonally induced with a single injection of hCG (2,000 IU/kg fish). Six groups were considered such as initial control, BSA‐implanted control, Vg1‐implanted, Vg2‐implanted, catfish collected from the field on the last day of the experiment and catfish collected during spawning period in this experiment with 3–7 fish in each group. Each of the experimental fish was sexually mature and the body weight was between 100 and 125 g. The percentage of ovulation and fertilization in the eggs of Vg1‐implanted group was 91% and 78%, respectively, which was almost similar to that of gravid female catfish collected during breeding period (July). The breeding performance in BSA‐ and Vg2‐treated females was very poor. The fertilized eggs were hatched in the laboratory conditions. Thus, in the female catfish, Vg1 not only induces vitellogenesis but also makes the oocytes viable for fertilization. 相似文献
17.
S. Mahapatra Sk. Kabita D. Bhattacharya S. Sarkar S. K. Juin S. Maitra P. Nath 《Fish physiology and biochemistry》2017,43(2):477-491
Two forms of vitellogenin (Vg: Vg1 and Vg2) were purified from the plasma of estradiol-17β (E2)-treated Indian walking catfish, Clarias batrachus, by gel filtration and adsorption chromatography. Native Vg1 and Vg2 had apparent molecular masses of 375 and 450 kDa, respectively, and both Vgs resolved into two similar major bands (95 and 67 kDa) in SDS-PAGE under reducing condition. Polyclonal antisera raised against each form of Vg were absorbed with a combination of hypophysectomized male catfish serum proteins and alternate Vg to ensure specificity. Immunological analyses verified the presence of Vg1 and Vg2 in the plasma of female catfish. Homologous ELISAs were developed for Vg1 and Vg2 using their respective harvested antisera, which exhibited the detection limit of 100 ng ml?1 for Vg1 and 40 ng ml?1 for Vg2, and low level of cross-reactivity (not parallel to the standard) was found with alternate Vg in each assay. Treatment of male catfish with E2 induced both Vgs showing a proportionate ratio of Vg1 to Vg2 at 5.6:1. Plasma concentrations of both Vgs measured by ELISAs at different reproductive phases of field collected female catfish increased in accordance with the ovarian development, keeping the proportionate ratio of Vg1 to Vg2 at about 2:1 in fish undergoing vitellogenesis during prespawning period and 1:20 during spawning period, suggesting that Vg1 may be the major Vg to contribute in yolk formation, whereas Vg2, besides its role in yolk formation, may facilitate other physiological functions. The present study, thus, demonstrates the occurrence of two unequally synthesized Vgs in the catfish. 相似文献
18.
Oocyte growth in most oviparous vertebrates including fish is due to the formation of yolk, and eggshell proteins (zona radiata
proteins). Zonagenesis leads to the formation of zona radiata proteins in oocytes, which play an important role during oogenesis,
whereas vitellogenesis leads to the formation of yolk in oocytes through a series of events during which the yolk precursor
protein vitellogenin (Vg) is synthesized and secreted from liver into blood from where it is sequestered into the developing
oocytes and thereafter proteolytically cleaved to form yolk proteins (YPs) and finally deposited in the ooplasm. Much research
has been done in many fish species with respect to the number and nature of Vg and YPs and their probable functions during
fish reproduction. Recent findings of multiplicity of Vg molecules in fishes reject the earlier view of a single-Vg model
and have led scientists to explore the functions of individual Vg and their YP derivatives, lipovitellin, phosvitin, and β′-component.
Two distinct types of Vg or Vg genes, containing or encoding the three YPs, have been detected in many teleosts. A third unusual,
incomplete, phosvitin-poor Vg has been described recently in many fishes. In comparison to much of the information on vitellogenesis
in many fishes very little is known for Indian fishes. In India research has been done in a few species such as the catfish,
Heteropneustes fossilis and Clarias batrachus, the murrel, Channa punctatus and the Indian major carps, Labeo rohita and Cirrhinus mrigala. Immunological and biochemical analyses suggest the occurrence of multiple forms of Vg and their YP derivatives. The synthesis
and incorporation of Vg are regulated by gonadotropin (GTH) and estradiol-17β (E2). A differential role between estrone (E1) and estriol (E3) has been demonstrated for Vg synthesis. Enzyme-linked immunosorbent assays (ELISAs) for Vg have been developed to measure
plasma Vg. Finally the different roles of Vg1 (HAI) and Vg2 (HAII) on vitellogenesis have been demonstrated. However, more
research remains to be carried out in other fish species with respect to the number and nature of Vg and YPs and their genes
in order to describe their reproductive functions. 相似文献
19.
Vitellogenin (Vg) of the barfin plaice Liopsetta pinnifasciata was isolated and purified. In native polyacrylamide gel electrophoresis, Vg appeared as one band. After being subjected to
sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE), Vg fraction produced several polypeptides with molecular
masses of 180, 98, 70, 52, 41 and 37 kDa. MALDI–TOF mass spectrometry (MS) of the 180- and 98-kDa Vg polypeptides from the
SDS–PAGE gel and de novo sequencing of their four peptide fragments based on MS/MS analysis confirmed that the purified proteins were vitellogenins,
which shared high similarity with the Vgs of the barfin flounder Verasper moseri and Atlantic halibut Hippoglossus hippoglossus. The most part of the predicted sequences obtained from the L. pinnifasciata 180-kDa polypeptide has previously been found in the V. moseri vitellogenin type B, the sequences obtained from the 98-kDa polypeptide were found in V. moseri vitellogenin type A, so these findings allow us to propose that L. pinnifasciata has at least two different forms of Vg. Rabbit polyclonal antibodies against Vg were produced, and a quantitative enzyme-linked
immunosorbent assay was developed. The concentration of Vg in barfin plaice from the moderately contaminated area of Amursky
Bay in the Sea of Japan was detected based on the maturity stage of their gonads. In November 2008, the Vg concentration in
the plasma of females with advanced oogenesis varied from 5.295 to 28.367 mg/ml (mean 16.38 ± 6.73 mg/ml, CV = 41.1%); in
the plasma of males, the concentration ranged from non-detectable to 0.957 mg/ml (0.29 ± 0.42 mg/ml, CV = 127.9%). In October
2009, the Vg concentration in female plasma was lower than in November 2008 (2.21–13.87 mg/ml). High individual variability
of plasma Vg was characteristic for maturing males (CV = 200.3%) and immature females (CV = 255.5%), and there was no significant
difference between plasma Vg concentrations in males captured in November 2008 and October 2009 or in maturing males and immature
females. Vacuolisation of hepatocytes was more typical for males with low plasma Vg concentrations and females with high plasma
Vg concentrations. Necrosis and pyknosis of hepatocyte nuclei were more frequent in males with high Vg concentrations and
in females with low plasma Vg concentrations. 相似文献
20.
The effects of aquarium background colour and feed colour on survival, growth rates and feed utilization efficiency of thinlip mullet (Liza ramada) larvae (0.035 g) were investigated in two experiments. In the aquarium background colour trial, 50 larvae were stocked in duplicates in 120 L glass aquaria filled with dechlorinated tap water. The outside walls and bottoms of each pair of the aquaria were covered with coloured paper sheets to achieve one of six colours (white, black, red, green, yellow and blue), while noncoloured aquaria served as a control. The fish were fed an experimental diet (35% crude protein) at a daily rate of 5% of their body weight (BW), twice a day for 8 weeks. The best growth rates, feed efficiency and survival were achieved in larvae reared in light‐coloured aquaria (white, noncoloured and yellow). Fish performance was significantly retarded in larvae reared in dark‐coloured aquaria (red, green, black and blue). Body composition was not significantly affected by aquarium colour. In a feed colour trial, duplicate groups of larvae (0.035 g) were stocked at 50 fish per 120 L aquarium and fed a test diet (35% crude protein) with six different colours [dark blue, red, yellow, light brown (control), light green and dark brown] at a daily rate of 5% BW, twice a day for 8 weeks. The best performance and survival were achieved in fish fed on dark‐coloured diets (red, dark blue and dark brown). Light‐coloured diets (yellow, light green and light brown) resulted in inferior performance. Body composition was not significantly affected by feed colour. These results suggest that light‐coloured tanks should be used for rearing thinlip mullet, L. ramada larvae, while dark‐coloured diets are more preferable to light‐coloured diets. 相似文献