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笔者从某猪场疑似猪魏氏梭菌病病例中分离到一株魏氏梭菌,为了研究其生物学特性及其发病机理,对分离菌进行了形态培养、生化试验、动物实验等鉴定,鉴定结果为C型魏氏梭菌.药敏试验表明本株魏氏梭菌对青霉素、磺胺药敏感性较高. 相似文献
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以A型魏氏梭菌贵州分离株为材料,提取染色体DNA,经PCR扩增和酚:氯仿抽提回收得到242bp的α-毒素基因部分片段,用地高辛标记制备了α-毒素基因探针。该探针不与埃希氏大肠杆菌、都柏林沙门氏菌、金黄色葡萄球菌、猪丹毒杆菌、蜡样芽胞杆菌及多杀性巴氏杆菌等细菌的DNA样品发生反应,只与魏氏梭菌DNA样品呈阳性反应,能检出300pg的DNA样品;应用探针对40株魏氏梭菌进行了Dot-Blotting检测,结果与生化试验鉴定的符合率达到90%,表明该探针具有较高的特异性和灵敏度,可应用于临床上魏氏梭菌的检测。 相似文献
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我国主要经济动物魏氏梭菌分离及血清型鉴定 总被引:8,自引:0,他引:8
对分离于7种经济动物的98株魏氏梭菌疑似物进行理化性质鉴定表明,分离菌符合魏氏梭菌特征。中和试验证实,分离于兔、狐狸、貉、水貂、麝鼠、犬的菌株均为A型;分离于鹿的菌株85.4%(35/41)为A型,14.6%(6/41)为C型,说明目前我国经济动物魏氏梭菌感染以A型为主。 相似文献
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《广东畜牧兽医科技》2015,(6)
从广东四会地区某猪场送检的病死猪肠管内容物中分离到1株疑似猪魏氏梭菌的细菌。经形态学观察、培养特性鉴定、生化试验、致病性试验,显示该菌为魏氏梭菌。药敏试验结果表明,该分离菌对庆大霉素、阿米卡星、卡那霉素等高敏,对四环素、氨苄西林、磺胺甲恶唑等耐药。 相似文献
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鸡腺胃型传染性支气管炎(综述) 总被引:15,自引:0,他引:15
1996年9月份以来,中国青岛及附近地区养鸡场20-90日龄蛋用育成鸡连续发生了一种以流泪,肿眼,伴有呼吸道症状,极度消瘦,拉稀,死亡为特征的一种新的呼吸道传染病。主要剖检表现为腺胃肿大如球状,腺胃壁增厚,腺胃粘膜出血溃疡,腺胃乳头平整融合,轮廓不清,可挤出脓性分泌物的腺胃炎症变化。本病病原通过鸡胚盲传5-6代,电镜观察到典型的冠状病毒。因为引起腺胃的特征性的病理变化,因此将此病暂定名为腺胃型传染性支气管炎。我们青岛地区鸡场的分离株定为QXIBV株。本篇从病原学、临床症状、发病机理和流行病学、免疫学、实验诊断和疾病控制方面进行综合论述。本病与肠型传支在理化特性、形态和毒力上相似,可能是同一种新的IBV变异株。 相似文献
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中国猪瘟兔化弱毒株(C-株)兔脾组织毒主要保护性抗原E2(gp55)基因序列分析 总被引:7,自引:2,他引:5
利用反转录(RT)及套式PCR(N-PCR)方法扩增了中国猪瘟兔化弱毒株(C-株)兔脾组织毒主要保护性抗原E2(gp55)基因,成功地将其克隆并测定了核苷酸序列,与国内外已发表的猪瘟病毒(HCV)E2基因序列比较的结果是C-株兔脾毒与C-株细胞(SK6)毒、C-株疫苗(犊牛睾丸细胞,HCLV-C)毒、HCV-SM株(石门)毒、Brescia株(荷兰)毒、Alfort株(德国)毒的E2核苷酸序列同源性分别为98.87%、98.34%、94.58%、91.00%、80.78%;氨基酸同源性分别为98.95%、97.37%、94.22%、91.60%、89.23%。对C-株兔脾毒与C-株细胞毒、经典强毒及国内流行野毒E2上的A、B、C三个中和性抗原区的氨基酸组成进行了比较,其结果为C-株兔脾毒与C-株细胞毒的差异很小甚至没有差异,而与流行野毒及经典强毒在B、C区有较大的差异。我国经典强毒石门毒与国内80年代和90年代流行毒之间有明显的差异,表明我国猪瘟流行毒株发生了变化。 相似文献
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牛体外牛黄发生器内培植牛黄技术研究 总被引:3,自引:1,他引:2
利用48头健康黄牛同时施行胆总管结扎术、胆囊插管术、十二指肠插管术和体外安装牛黄发生器,将原胆汁流通径路改建为肝脏→胆管→胆囊→引胆汁管→牛黄发生器→十二指肠,在体外牛黄发生器内和胆囊内同时培植牛黄。结果表明,植黄牛胆汁pH值降低,胆酸含量下降,牛黄发生器内胆汁粘度低于胆囊内胆汁粘度。胆囊内注入新洁尔灭,可减少胆汁中细菌数量,减轻胆囊炎症反应。全棉材料牛黄床的牛黄产量高于锦纶66和涤纶材料牛黄床,以网眼状全腔充盈型牛黄床的牛黄产量高、质量好。在5~7个月的培植期内,每头牛能生产牛黄14.1~19.6g,牛黄胆红素含量为29.05%±7.51%。 相似文献
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The use of polymerase chain reaction for determination of virulence factors of Escherichia coli strains isolated from pigs in Poland. 总被引:5,自引:0,他引:5
J Osek P Gallien M Truszczy?ski D Protz 《Comparative immunology, microbiology and infectious diseases》1999,22(3):163-174
E. coli strains isolated from pigs with postweaning diarrhea or edema disease were tested by phenotypic and genotypic methods for the presence of virulence antigens and genes, respectively. The slide agglutination and ELISA analyses were used for determination of F4, F5, F6, F17, and F41 fimbriae whereas the prevalence of fimbrial fedA and toxin eltI, estI, estII, stx1, stx2 and stx2e genes were recorded by the means of PCR. Only F4 antigen (ac variant) was found in strains of the serogroup O149:K91 isolated from pigs with diarrhea. PCR analyses showed that the fedA gene encoding F18 fimbriae was present in 61.9% of strains isolated from pigs with diarrhea and in 84.2% of strains isolated from pigs with edema disease. The eltI genes encoding heat-labile toxin I (LTI) were present only in 9 out of 21 strains recovered from pigs with diarrhea. Shiga toxin 2 variant (stx2e) genes were found in six isolates from edema disease and also in one strain from diarrhea. The PCR test used in the study was a sensitive and valuable method for determination of virulence factors of E. coli strains. 相似文献
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[目的]研究分光光度法测定牛乳中亚硝酸盐含量的不确定度评定。[方法]根据《GB 5009.332016食品安全国家标准食品中亚硝酸盐与硝酸盐的测定》中的第二法分光光度法来测定牛乳中亚硝酸盐的含量。通过分析不确定度的来源,建立完整的数学模型,对模型中的各个分量进行分析和评定,合成相对标准不确定度,并对合成的相对标准不确定度进行扩展,得出牛乳中亚硝酸盐含量的扩展不确定度。[结果]当取置信度为95%时,得到的牛乳中亚硝酸盐含量:X=(0.058±0.0034)mg/kg,取k=2,扩展不确定度为U=0.0034 mg/kg。[结论]试验过程的不确定度主要来源于标准工作溶液配制过程、试样处理液总体积、测定样液定容体积和样品的重复性。该方法可以为分光光度法测定牛乳中亚硝酸盐含量的不确定度评定提供参考依据。 相似文献
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从五指山近交系 (WZSP)培育群中 ,先后选用自然或同期发情 1 1头 5 8月龄青年母猪 ,分别于授精后 2 5~ 2 9d采集胎儿 75个 ,进行原始生殖嵴 (PGCs)细胞分离、培养等建系技术研究。培养液为DMEM F1 2 ( 1∶1 ) ,并含 1 5 %胎牛血清、0 1mmol/L硫基乙醇、40ng/mlhSCF、2 0ng/mlhLIF、2 0mmol/L谷胱甘肽 ,另添加或不添加 2 0ng/mlbFGF作为对比试验。用STO细胞作饲养层 ,在 37 5℃、5 0 %CO2和湿润的气相中进行培养。分别冷冻保存胚胎生殖嵴细胞 (EG)细胞系 1 1个 ,其中 2个EG… 相似文献
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表达马立克氏病病毒CVI988/Rispens株糖蛋白B基因的重组鸡痘病毒疫苗的免疫保护研究 总被引:1,自引:0,他引:1
用鸡痘病毒载体表达马立克氏病病毒(MDV)糖蛋白B(gB)基因构建成重组鸡痘病毒(rFPV)。以MDV GA或Md5和RBIB混合攻毒,在不同品种的鸡中评价rFPV-gB/R单价苗和与火鸡疱疹病毒(HVT)组成的二价苗的免疫保护效力。试验结果表明,MDV疫苗和FPV母源抗体阴性的SPF鸡和母源抗体阳性的商品鸡中,rFDV-gB/R均能提供免疫保护作用,且其中一种商品蛋鸡中rFPV-gB/R与HVT液氮苗或HVT冻干苗结合使用,均有显著的免疫协同保护作用。免疫学研究指出,rFPV-gB/R与常规疫苗一样,可显著地降低疫苗免疫攻毒鸡的病毒血症和羽囊排毒。 相似文献
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Starcic M Johnson JR Stell AL van der Goot J Hendriks HG van Vorstenbosch C van Dijk L Gaastra W 《Veterinary microbiology》2002,85(4):361-377
Twenty-four haemolytic Escherichia coli strains were isolated from dogs with diarrhea. The strains were serotyped and analysed by polymerase chain reaction (PCR) for genes encoding virulence factors associated with E. coli that cause diarrhea in animals. Adhesion antigen production was deduced from haemagglutination experiments. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of heat extracts was also used as an indication for the production of adhesive structures. The majority of the strains was shown to produce this type of virulence factor. Adhesion and invasion tests of the strains and Caco-2 cells showed that all strains adhered and that two were invasive. The two invasive strains were positive in the intimin PCR and one of them also contained genes encoding CS31A. The PCR for heat stable toxin (ST) was positive in only four strains, as was the presence of F17 fimbrial genes. Surprisingly, 19 strains had intact P fimbrial operons, coding for an adhesin involved in urinary tract infection (UTI). The cytotoxic necrotising factor 1 (CNF1) gene, also mainly found in UTI was likewise detected in these 19 strains. Cytolethal distending toxin (Cdt) genes were found in five strains. The high number of strains positive for CNF1 and P fimbriae prompted us to test the strains in a multiplex PCR used to test E. coli isolated from UTI in various species for 30 virulence associated genes. The data showed that the majority of the diarrhea isolates have virulence factor profiles highly similar to UTI E. coli isolates from dogs. This raises the question whether these isolates are real intestinal pathogens or "innocent bystanders". However, since CNF1 producing necrotoxic E. coli (NTEC) strains isolated from humans, pigs and calves with diarrhea appear to be highly related to our strains, it might be that in dogs this type of isolate is capable of causing not only UTI, but also diarrhea. If this is the case and this type of isolate is "bifunctional", domestic animals likely constitute a reservoir of NTEC strains which can be also pathogenic for humans. 相似文献