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草鱼出血病的防治汪占华（河南省新野县畜牧兽医站４７３５００）王三忠（河南省新野县水利局渔场４７３５００）草鱼出血病病原体是呼肠孤病毒，每年６～１０月为流行季节，以８～９月最甚。为害对象是当年草、青鱼种。该病主要症状为：肌肉、口腔、各种鳍条基部出血，尤... 相似文献
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陈如梅 《养殖与饲料．饲料世界》2015,(4):51-53
<正>随着养殖业的不断发展,水产养殖在给广大养殖户带来较大经济效益的同时,出现的各种流行疾病也给养殖业带来了不小的冲击。笔者从事鱼病防治多年,积累了一定的经验,以下重点分析几种淡水养殖鱼类中较为流行的病害,并提出防治措施。1草鱼出血病1.1病因该病主要由水生呼肠孤病毒引起。1.2流行情况本病的高发期为每年6-9月份,主要危害2.5~15.0 cm草鱼种和1龄青鱼,水温在27℃以 相似文献
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研究以纯化的羊抗草鱼呼肠孤病毒多克隆抗体作为捕获抗体,抗草鱼呼肠孤病毒的单克隆抗体为检测抗体,建立草鱼呼肠孤病毒抗原捕获ELISA检测方法,其最佳反应条件是：羊多克隆抗体包埋浓度为2.5μg/mL,抗草鱼呼肠孤病毒单克隆抗体工作浓度为1:400稀释,以3％牛血清白蛋白作为封闭液。该方法的检测限为5×105 pfu/mL,且能够特异性检出发病草鱼内脏组织中的草鱼呼肠孤病毒。特异性试验结果表明该方法具有较高的特异性,与病毒性出血性败血症病毒、传染性造血器官坏死病毒和鲤春血症病毒等水产动物病毒株均不反应。该方法还具有较好的稳定性。 相似文献
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《兽药与饲料添加剂》2004,(2)
草鱼出血病属呼肠孤病毒科中一种水生生物呼肠孤病毒。此病是目前草鱼饲养阶段危害最大的一种传染性疾病,它流行于全国各养殖地区,其中以长江流域和广西、广东、福建等主要养鱼地区流行最为严重。流行季节在6月下旬到9月底,特别是7月中旬到9月上旬,水温在27℃以上时最为流行,水 相似文献
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<正>草鱼出血病是鱼种培育阶段一种流行地区广泛、流行季节长、发病率高、死亡率高、危害性大的病毒性鱼病。病原体是两种病毒颗粒,一种是呼肠孤病毒(双股RNA病毒),另一种是20～30nm大小的病毒(单股RNA病毒)。该病主要流行于长江流域和珠 相似文献
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为了解循环流水槽养殖草鱼与池塘精养草鱼营养品质的差异,选取相同饲料源的循环流水槽养殖草鱼[平均体重:(723.89±71.54)g,平均体长:(340.86±8.88)mm]和池塘精养草鱼[平均体重:(680.35±155.13)g,平均体长:(334.00±21.40)mm]各12尾,测定形态指标与肌肉剪切力、质构特性、色泽以及常规营养成分、氨基酸、脂肪酸和矿物元素含量。结果显示:1)循环流水槽养殖草鱼的硬度、黏性、咀嚼性、回复性和剪切力均显著高于池塘精养草鱼(P<0.05)。2)循环流水槽养殖草鱼的肌肉水分含量显著低于池塘精养草鱼(P<0.05),肌肉粗脂肪含量低于池塘精养草鱼,但差异不显著(P>0.05),肌肉粗蛋白质和粗灰分含量均高于池塘精养草鱼,但差异不显著(P>0.05)。3)循环流水槽养殖草鱼的肌肉氨基酸总量(TAA)、必需氨基酸总量(TEAA)和呈味氨基酸总量(TDAA)高于池塘精养草鱼,但差异不显著(P>0.05)。循环流水槽养殖草鱼和池塘精养草鱼肌肉中TAA分别为77.11%和76.61%,TEAA和TDAA分别占TAA的40.87%和40.43%、46.10%和46.06%。4)循环流水槽养殖草鱼肌肉中不饱和脂肪酸(UFA)占脂肪酸总量的百分比(69.68%)高于池塘精养草鱼(68.44%),但差异不显著(P>0.05)。循环流水槽养殖草鱼和池塘精养草鱼肌肉中饱和脂肪酸(SFA)占脂肪酸总量的百分比分别为21.78%和18.58%,两者差异显著(P<0.05)。循环流水槽养殖草鱼肌肉中未检测到二十碳五烯酸(EPA),二十二碳六烯酸(DHA)含量为0.44%;池塘精养草鱼肌肉中EPA含量为0.10%,DHA含量为0.55%。5)循环流水槽养殖草鱼肌肉中钾、镁、铁、铜含量显著高于池塘精养草鱼(P<0.05),钠、锌显著低于池塘精养草鱼(P<0.05)。由此得出,循环流水槽养殖草鱼的营养品质总体上优于池塘精养草鱼。 相似文献
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E. M. Yu B. H. Liu G. J. Wang D. G. Yu J. Xie Y. Xia W. B. Gong H. H. Wang Z. F. Li N. Wei 《Journal of animal physiology and animal nutrition》2014,98(4):755-765
Grass carp (Ctenopharyngodon idellus) are important Chinese freshwater fish, and in China, the faba bean has been used as the sole food source for grass carp to transform them into crisp grass carp. Because of this, crisp grass carp has become an economically important fish because of its increased muscle hardness. To study the nutritional regulation of type I collagen in faba bean‐fed grass carp, we isolated type I collagen alpha 2 (COL1A2) on the basis of our isolation of COL1A1. The COL1A2 cDNA was found to be 4899 bp in length and included a 4059‐bp coding sequence (CDS) and encoded a polypeptide of 1352 AA. The protein peptide molecular weight was 127.39 kD, and the theoretical isoelectric point was 9.37. The COL1A2 protein possessed five α‐helixes, eight β‐sheets, 16 regions of triple helical repeats, 21 low‐complexity regions, 10 function domains and two zinc‐binding sites; however, no calcium‐binding sites were observed. The mRNA expression of COL1A1 and COL1A2 was assessed in eight tissues (muscle, hepatopancreas, intestine, gills, skin, fin, kidney and spleen) from grass carp and crisp grass carp by semi‐quantitative RT‐PCR. Expression of COL1A1 in the muscle, intestines and skin of crisp grass carp was higher than that in grass carp, and expression of COL1A2 in the muscle, gills, fin and skin of crisp grass carp was higher than that in grass carp. In the muscle of crisp grass carp, expression of COL1A1 and COL1A2 was higher than that in grass carp, which was further confirmed by real‐time PCR, and collagen content also was enhanced. These results demonstrated that type I collagen was closely related to the increased muscle hardness of faba bean‐fed grass carp. 相似文献
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牛磺酸对草鱼生长效应研究 总被引：5，自引：1，他引：4
在饲料中添加0、200、400、600、800、1000、1400、1800mg/kg的牛磺酸,饲养草鱼种30d,研究牛磺酸对草鱼的生长效应。结果表明:①牛磺酸促进草鱼生长(除1800mg/kg添加量外)。添加量为600mg/kg时,特定生长率(SGR)、饲料转化率(FCR)和蛋白质效率表现为最好;②饲料中添加牛磺酸后,草鱼体水分含量下降,体脂增加,体蛋白含量增加,灰分含量、内脏/体重比、肥满度各试验组之间差异不显著。根据生产性能指标综合评定,我们建议草鱼饲料中牛磺酸最适添加量为600mg/kg。 相似文献
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采用同位素示踪法和肠道离体灌流模型研究了鲫鱼和草鱼肠道对酪氨酸(Tyr)、脯氨酸(Pro)的吸收量、吸收速率、跨壁运输量与氨基酸灌流时间和灌流浓度的关系。结果表明:随着Tyr、Pro灌流浓度的增加,鲫鱼、草鱼肠道的吸收速率与其浓度均呈正相关关系。但鲫鱼对Tyr、Pro的吸收转运速度明显高于草鱼。随着鲫鱼、草鱼肠道内Tyr、Pro灌流的浓度逐渐下降,留存于其肠道的两种氨基酸的比例也随之下降,而通过肠道转运到肠道外的比例则逐渐增加,但草鱼转运到肠道外的比例极显著低于鲫鱼(P<0.01)。一般,鲫鱼肠道对Tyr、Pro的总吸收率随着灌流氨基酸浓度的下降而下降,草鱼肠道对Tyr总的吸收率随着灌流氨基酸浓度的下降而呈上升趋势。对Pro的总吸收率以5mmol.L-1组(8.14%)最高。且鲫鱼对Tyr、Pro的总吸收率低于草鱼相应的氨基酸浓度组。 相似文献
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Fumiao Zhang Ranran Feng Wei Fang Yanhui Shi Liguo An Guiwen Yang 《Anatomia, histologia, embryologia》2019,48(1):22-32
Fish are the most diverse species of all vertebrate groups, and their blood cells have shown variable characteristics in terms of morphology. Cytochemical staining for enzyme activity in blood leukocytes will help assess the immune function of fish. We characterize blood cells from crucian carp (Carassius auratus) and grass carp (Ctenopharyngodon idellus) by using a Diff‐Quick stain as well as different cytochemical methods. Blood specimens obtained from crucian carp and grass carp were evaluated after cytochemical staining for acid phosphatase (ACP), alkaline phosphatase (ALP), naphthol AS chloroacetate esterase (AS‐DNCE), naphthyl acetate esterase (NAE), α‐naphthyl butyrate esterase (NBE), peroxidase (MPO) and periodic acid–Schiff's reaction (PAS) using commercial kits. Blood cell types were evaluated based on their morphological characteristics and the presence or absence of specific chromogen. The expression pattern of enzymes was similar between the two Cyprinidae and was also broadly consistent with other fish species. However, there were some interesting differences detected between crucian carp and grass carp, including naphthol AS chloroacetate esterase activity in monocytes, peroxidase activity and location in thrombocytes. The ACP, ALP and MPO expressions of different leukocytes of the two Cyprinidae were evaluated by Image Pro Plus and were analysed for statistical significant differences. This investigation provides basic haematology and enzyme activity analyses for crucian carp and grass carp and serves as an approach to evaluating the immune response of fish. 相似文献
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试验采用光镜和透射电镜技术,探讨了不同浓度氟化钠对草鱼肝脏组织显微和超微结构的影响。氟化钠暴露浓度分别为0、40、80、120 mg/L,每组设2个平行,连续暴露30 d,取肝脏组织制备切片,进行光镜和透射电镜观察。结果发现,30 d后氟化钠试验组草鱼肝脏组织均有不同程度的损伤,石蜡切片上可观察到试验组草鱼肝细胞索紊乱、断裂,细胞出现空泡变性且病变程度随氟化钠浓度升高而加重,透射电镜下可观察到试验组草鱼肝细胞线粒体轻度肿胀,脊模糊或缺失,线粒体数量减少,胞质中出现空泡。结果表明,氟化钠短期暴露可对草鱼肝脏组织造成损伤,且损伤程度存在剂量依赖效应。 相似文献
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为了考察姜黄素对草鱼肝损伤的修复作用,选用患有肝胆综合症草鱼为研究对象,以草鱼肝组织形态及血清生化指标为主要考察指标,进行了姜黄素的肝损伤修复作用初步研究.试验结果显示,姜黄素拌饲投喂7d后,给药组、对照药组与空白对照组相比,肝功能相关的生化指标(MDA、AST、ALT、LDH、T-SOD、CAT、GSH-PX)以及肝脏病理得到较为显著改善.结果表明,姜黄素对草鱼肝损伤具有一定的修复作用. 相似文献