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RP-HPLC法检测安替比林血药浓度 总被引：1，自引：0，他引：1
建立了安替比林血药浓度检测的反相高效液相色谱法(RP-HPLC).血浆经乙腈提取后进样测定,色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),柱温为30℃,流动相为0.025 mol/L醋酸钠溶液-乙腈(74:26,V/V),流速为0.8 mL/min,检测波长为254 nm,进样量20μL.安替比林血药浓度在0.1～30μg/mL范围内线性关系良好(r=0.999 5),平均回收率为93.44%～97.21%,日内和日间变异系数小于4.26%. 相似文献
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应用YWG-C18色谱柱(250 mm×4.6 mm,5μm),WatersTM 486型可调波长紫外检测器(检测波长265 nm);0.01 M磷酸钾(pH=7)乙腈(3 1)为流动相;含量测定采用标准曲线法,建立了RP-HPLC法检测绵羊血浆中克洛素隆含量的方法.同时对绵羊单剂量静脉注射7 mg/kg克洛素隆的药代动力学进行了研究.血药浓度在0.005～2.0 μg/ml及2.0～50.0μg/ml范围呈良好线性关系(r=0.9941、0.9970),平均回收率93.2%,血药最低检测浓度0.005μg/ml,日内日间变异系数分别小于10%、11%.血药浓度结果经MCPKP药代动力学统计软件处理,体内药物运转符合三室开放模型,主要药物动力学参数t1/2γ=14.14 h,Kel=0.3260 h-1,AUC=153.95 mg/l@h,fc=0.055.结果表明克洛素隆静脉单次给药后体内分布较为广泛,持续作用时间长,主要通过肾脏排泄. 相似文献
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HPLC法测定麻黄鱼腥草散中黄芩苷、穿心莲内酯、脱水穿心莲内酯的含量 总被引：1，自引：0，他引：1
为了建立HPLC法测定麻黄鱼腥草散中黄芩苷、穿心莲内酯、脱水穿心莲内酯含量的方法,采用色谱柱Waters XBrige~(TM)C18(4.6 mm×150 mm,5μm),黄芩苷以甲醇-0.2%磷酸溶液为流动相梯度洗脱,流速为1.0 m L/min,柱温:30℃;穿心莲内酯、脱水穿心莲内酯以甲醇-水(50∶50)为流动相,流速:1.0 m L/min,柱温为30℃;黄芩苷、穿心莲内酯、脱水穿心莲内酯检测波长分别为276、225、254 nm。黄芩苷的线性范围为0.0798~0.7975μg(r=1.0000),平均回收率为94.3%(RSD=0.8%)。穿心莲内酯的线性范围为0.0109~0.2175μg(r=1.0000),平均回收率为92.9%(RSD=0.7%);脱水穿心莲内酯的线性范围为0.0105~0.2105μg(r=0.9999),平均回收率为92.4%(RSD=0.3%)。本方法快速、简便、准确,能有效测定麻黄鱼腥草散中穿心莲内酯、脱水穿心莲内酯的含量。 相似文献
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紫外系数倍率法测定盐酸咪唑苯脲缓释剂在健康奶牛中的血药浓度 总被引：1，自引：0，他引：1
本试验以261 nm为测定波长,277 nm为参比波长,采用紫外系数倍率法测定盐酸咪唑苯脲缓释剂在健康奶牛体内不同时间的血浆药物浓度,并用药动学计算软件拟合处理得药动学参数。结果表明:此方法能不经分离直接消除血浆的背景干扰,盐酸咪唑苯脲标准品在0.1~5.0μg/mL的范围与ΔA呈良好的线性关系(r=0.999 9),平均回收率96.16%,日内、日间RSD%均小于5.00%。药-时曲线符合一室吸收模型,药动学方程为:C=2.254 0(e-0.008 8t-e-0.422 3t),r=0.998 0。与肌肉注射盐酸咪唑苯脲水剂相比,消除半衰期(t1/2ke)延长了75.402 h,药物峰浓度时间(Tmax)延长了7.631 h。盐酸咪唑苯脲缓释剂优于盐酸咪唑苯脲水剂。 相似文献
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本试验以261nm为测定波长,277nm为参比波长,采用紫外系数倍率法测定盐酸咪唑苯脲缓释荆在奶牛体内不同时间的血浆药物浓度,并用药动学计算软件拟合处理得药动学参数.结果表明:此方法能不经分离直接消除血浆的背景干扰,咪唑苯脲标准品在0.1～5.0μg/mL的范围与△A呈良好的线性关系(r=0.9999),平均回收率96.16%,日内、日间RSD(%)均小于5.00%.药时曲线符合一室吸收模型.药动学方程为:C=2.2540(e-0.0088t-e-0.4223t),r=0.9980.与肌肉注射咪唑苯脲水剂相比,消除半衰期[T1/2(Ke)]延长了75.402h,药物峰浓度时间[Tmax]延长了7.631h.咪唑苯脲缓释剂优于咪唑苯脲水剂. 相似文献
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实验猪6头,单剂量(20mg/kg)静脉注射国产硫酸安普霉素,用微生物法测定血清药物浓度,研究其在猪体内的药代动力学.结果平均回收率为99.03%,血清最低检测浓度为0.05μg/ml,日内日间变异系数为2.2%～5.1%,且血清浓度在0.05～3.00μg/ml范围呈良好线性关系(r=0.9965).猪静脉注射硫酸安普霉素血药浓度,经Mcpkp药代动力学计算机程序处理,体内药物运转符合开放型二室模型,t1/2c(0.75士0.21)h,t12β(3.019±0.67)h,CLB为(0.16士0.01)1/kg·h 相似文献
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用HPLC法同时测定氯胺酮、地西泮血药浓度及药代动力学。流动相:乙腈∶水(用Na3PO4调pH≈10)=1∶1(V/V),紫外检测波长:UV=230 nm;流量:0.6 mL/min,建立测定条件。绘制标准曲线,地西泮回归方程为y=1 873.4x-9 421,R2=0.999 6,在0.25～8μg/mL范围内呈现良好的线性关系;氯胺酮回归方程为y=75 586x-25 286;R2=0.999 5,在1～32μg/mL范围内呈现良好的线性关系。单次静脉给药后,分别于0、5、10、20、40及60 min测定血药浓度,并计算两者药代动力学参数。 相似文献
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二氟沙星残留检测的Ci-ELISA方法建立 总被引：1，自引：0，他引：1
以合成的二氟沙星（DIF）人工抗原为基础，制备出了高效价抗DIF血清（1∶29×104），并建立了DIF间接竞争酶联免疫吸附测定法(Ci-ELISA法)。标准曲线的线性回归方程为y=-0.223x+0.9631(r=0.9929)，中值（I50）=119.21 ng/ml，最低检测限（LOD）=1.14 ng/ml，线性范围为1.14~10000 ng/ml，方法的平均批内变异系数（CV）为4.83%,平均批间CV为10.69%。DIF以浓度15~10000 ng/ml在鸡肉中添加时，回收率为81.24%~90.64%，变异系数为8.18%~11.75%。 相似文献
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目的:建立一种高效、准确、快捷的人体血浆中替加氟浓度检测方法。方法:色谱柱:Pronaos EP-C18柱;流动相:甲醇-乙腈-水(10∶5∶85);流速:1.0 mL/min;进样量20μL;柱温25℃;检测波长:λ=270 nm。结果:本法在替加氟浓度(X)为0.415~166μg/mL(r=0.999 9)内线性关系良好。低、中、高3种不同浓度(8.3μg/mL、41.5μg/mL、207.5μg/mL)相对回收率分别为99.64%、97.78%、99.41%,日内、日间精密度的RSD均小于5%。结论:此法用血量少,血浆处理方法简单,具有专一性,适用于临床替加氟血药浓度的监测。 相似文献
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Ovine scrapie was first recorded in Cyprus in 1985. Subsequently four dairy goats kept in two mixed flocks with affected sheep developed characteristic clinical signs similar to those seen in sheep. Fifteen goats from the two flocks were examined histologically and neurological lesions consistent with a diagnosis of scrapie were found in the four animals and in three others which had subsequently developed early neurological signs. These lesions were similar to those of naturally-affected sheep although neuronal degeneration and vacuolation were more severe in some cases. 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献14.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献16.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
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In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
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Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献