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1.
Apolipoprotein E (apoE) is a protein constituent of lipoproteins, and acts as a receptor-binding ligand. Although the existence of bovine apoE in lipoprotein fractions has already been reported, quantitative studies on the changes of apoE in plasma and lipoprotein fractions are lacking. In the present study, an increase of a 38 kDa protein in the very low-density lipoprotein (VLDL) fraction obtained from fasted calves was detected. This 38 kDa protein was identified as bovine apoE by determination of the N-terminal amino acid sequence. Bovine apoE was purified and an enzyme-linked immunosorbent assay (ELISA) was developed. Using this system, the effect of fasting on the concentration of apoE in plasma and the distribution of apoE in lipoprotein fractions were investigated. After 3 days of fasting, the concentration of plasma apoE increased significantly (p<0.05) by 280 %, and was returned to the basal level by 3 days of refeeding. The lipoprotein fractions obtained from before and after fasting was separated by ultracentrifugation. ApoE was significantly increased in VLDL, low-density lipoprotein (LDL) and non-lipoprotein fractions by fasting (p<0.05). On the other hand, in high-density lipoprotein (HDL) fractions obtained from both before and after fasting, the level of apoE was very low compared to the other fractions. These results suggested that bovine apoE contents in triglyceride-rich lipoproteins are modulated by nutritional treatment and closely associated with triglyceride-rich lipoprotein metabolism.  相似文献   

2.
The hepatic mitochondrial carnitine palmitoyltransferase (CPT) activity was measured by fluorimetric assay in dairy cows with or without fatty liver. CPT activities in 13 lactating cattle and in 6 non-lactating cows were 304.4±86.6 mol CoA/min per g protein and 169.3±84.8 mol CoA/min per g protein, respectively. This difference was significant (<0.05). CPT activities in early lactation (0–110 days after calving), mid-lactation (111–220 days after calving) and late lactation (over 220 days after calving) were 278.9±68.0, 312.4±124.1 and 320±59.3 mol CoA/min per g protein, respectively. There was no significant difference between the values at different stages of lactation. The CPT activity in 10 lactating cows with fatty liver unrelated to calving was 201.3±80.0 mol CoA/min per g protein. CPT activity in 10 cattle with fatty liver was significantly lower than that in normal lactating cattle. Based on these findings, clinical fatty liver unrelated to calving appears to be associated with a decrease in hepatic CPT activity.  相似文献   

3.
Dairy cows rely on a complex ruminal microbiota to digest their host-indigestible feed. Our ability to characterize this microbiota has advanced significantly due to developments in next-generation sequencing. However, efforts to sample the rumen, which typical y involves removing digesta directly from the rumen via a cannula, intubation, or rumenocentesis, is costly and labor intensive. As a result, the majority of studies characterizing the rumen microbiota are conducted on samples col ected at a single time point. Currently, it is unknown whether there is significant day-to-day variation in the rumen microbiota, a factor that could strongly influence conclusion drawn from studies that sample at a single time point. To address this, we examined day-to-day changes in the ruminal microbiota of lactating dairy cows using next-generation sequencing to determine if single-day sampling is representative of sampling across 3 consecutive days. We sequenced single-day solid and liquid fractions of ruminal digesta col ected over 3 consecutive days from 12 cannulated dairy cows during the early, middle, and late stages of a single lactation cycle using the V4 region of the bacterial 16 S r RNA gene. We then generated 97% similarity operational taxonomic units(OTUs) from these sequences and showed that any of the individual samples from a given 3-day sampling period is equivalent to the mean OTUs determined from the combined 3-d data set. This finding was consistent for both solid and liquid fractions of the rumen,and we thus conclude that there is limited day-to-day variability in the rumen microbiota.  相似文献   

4.
This study utilized three staining assays (Annexin V, mitochondrial membrane potential (JC‐1) and TUNEL) for flow cytometric analysis of apoptosis in sex‐sorted sperm from four different bulls (A, B, C and D). Correlations between sperm quality and IVF efficiency were then assessed to determine which assay provided the best prediction of IVF efficiency. The results of the Annexin V assays, as well as measures of viable sperm, early apoptosis, necrotic sperm and mitochondrial membrane potential (?ψm) showed that the sex‐sorted sperm collected from bull A significantly differed from those of the other three bulls (p < 0.05). In addition, the levels of DNA fragmentation in sex‐sorted sperm from bull A were significantly lower than those from bulls B and C (p < 0.05). The percentage of cells reaching the cleavage and blastocyst stages in sex‐sorted sperm from bull A were significantly greater than those from the other bulls (p < 0.05). A significant positive correlation was observed between viable sperm and the percentage of cells at the cleavage or blastocyst stages (p < 0.05). In contrast, a negative correlation was found between early apoptotic sperm and the percentage of cells at the cleavage or blastocyst stages (p < 0.05). In conclusion, these results indicated that the Annexin V assay was the most reliable technique for the prediction of the IVF success of sex‐sorted bovine sperm.  相似文献   

5.
为研究不同中性洗涤纤维/非纤维性碳水化合物(NDF/NFC)饲粮对泌乳后期奶牛甲烷排放量、营养物质消化率和生产性能的影响,试验选用体重(777.46±27.31)kg、胎次(1.5±0.15)胎、泌乳天数(242.92±15.28)d、产奶量(18.75±0.62)kg/d的奶牛12头,随机分配到3组,每组4头。各组饲粮NDF/NFC分别为2.10(精粗比为42:58)、1.96(精粗比为37:63)、1.52(精粗比为30:70),采用六氟化硫(SF_6)示踪技术测定自然状态下泌乳奶牛甲烷排放量。预试期14 d,正试期9 d。结果表明:饲喂不同NDF/NFC饲粮的泌乳后期奶牛甲烷排放量、甲烷能、单位干物质采食量的甲烷排放量以及单位总能摄入量的甲烷能均有显著差异;随着NDF/NFC降低,奶牛生产性能、饲料转化率和营养物质消化率无显著差异。综上,在不影响奶牛健康和生产性能的前提下,NDF/NFC为1.52的低NDF组饲料能显著降低泌乳后期奶牛瘤胃甲烷排放量。  相似文献   

6.
Fertility of primiparous lactating does in the early postpartum (pp) period is very low mainly due to pronounced deficient energy intake, influencing oocyte and embryo developmental competence. The hypothesis used in this work was that high‐lignin fibre diet supplied during the rearing period could increase feed intake and, consequently, improve the reproductive physiology and metabolic status of primiparous does in the early pp period. Diets with high‐lignin [HL: 15.8% dry matter (DM)] or standard‐lignin content (SL: 4.9% DM) were supplied until parturition time. No diet effects in serum oestradiol, progesterone concentrations and follicle categories were found in the histological study. Metaphase II rate of in vitro‐matured oocytes was significantly higher in the SL vs the HL group (p < 0.001). Cytoplasmically degenerated oocytes (in terms of abnormal distribution of cortical granules) and follicular atresia rate were significantly lower in the SL group than in the HL group (p < 0.05 and p < 0.005 respectively). In addition, HL‐fed does showed lower number of viable embryos and higher rate of retarded in vivo‐recovered embryos compared with the SL group (p < 0.05). Neither in vitro embryo development of viable embryos nor conception rate was significantly different between groups. Feed intake increased during the first pregnancy in the HL group (p < 0.05), but not during early lactation. Serum protein, non‐esterified fatty acid and leptin concentrations, as well as estimated body composition were similar in does fed with both diets. In conclusion, the enhancement of reproductive management by using highly lignified products in rearing diets does not seem to report physiological reproductive benefits affecting oocyte maturation rate and embryo viability in primiparous lactating does.  相似文献   

7.
The impact of pregnancy on ketone body disposal during a hyperketonaemic clamp was examined by tracer isotope dilution techniques in seven 12 h fasted sheep in three reproductive states, in the dry non-gestating period, late in gestation and during early lactation. After a sampling period of 60 min under basal conditions a DL-BHB racemate solution was continuously infused intravenously for 3 h at rates of 14.3-24.3 micromol/(kg min) to elevate the D-BHB concentration in blood plasma to values between 5 and 7 mmol/l. Two separate experiments were carried out with the same sheep in each of the three reproductive states. During pregnancy three ewes were pregnant with a single lamb and four ewes carried twins. Maximal D-BHB turnover rates fell significantly in late gestation by 26% relative to early lactation and by 22% when compared with the dry non-pregnant state. Reduction of maximal D-BHB disposal rate during late gestation was accompanied by a significant 297% (p<0.005) and a non-significant 49% increase in the basal BHB concentration in blood, a non-significant 10% and 4% decrease in the rate constant of D-BHB turnover and a non-significant 24% and 13% rise in the incremented increase of D-BHB concentration per unit D-BHB infusion, relative to the dry and the lactating period, respectively. Induction of hyperketonaemia significantly lowered NEFA and glycerol concentrations in blood by 67% and 57%, respectively, compared to the pre-infusional concentrations. The magnitude of this effect was the same in all reproductive states and was explained as a direct antilipolytic action of D-BHB on adipose tissue. It is concluded that the reduced ability of the late gestating ewe to utilize D-BHB promotes hyperketonaemia. Since hyperketonaemia exerts several adverse effects, e.g. on energy balance and glucose metabolism it appears that the impairment of ketone bodies disposal in late pregnancy facilitates development of pregnancy toxaemia, especially in ewes carrying twins.  相似文献   

8.
The concentrations of lactoferrin (Lf) in quarter milk from normal lactating cows and subclinical mastitic cows were measured to determine whether the Lf concentration in milk is influenced by the age of the cow, the stage of lactation, number of milk somatic cells and the presence of pathogens. Lf concentrations in 111 quarter milk samples from 28 normal lactating cows and 270 quarter milk samples from 198 subclinical mastitic cows were measured by means of a single radial immunodiffusion test. Lf concentrations (means +/- standard deviations; logarithmic form) in normal cows and subclinical mastitic cows were 2.23 +/- 0.39 and 2.70 +/- 0.39, respectively. The mean milk Lf concentration (log) in subclinical mastitic cows was significantly (p<0.01) higher than that in normal cows. The mean milk Lf concentration (log) in normal lactating cows aged 5 years was lower than those in normal lactating cows aged 2 years (p<0.01) and 3 years (p<0.05). The results showed that the milk Lf concentration (log) is associated with age of the dairy cow (one-way analysis of variance test, p<0.01). The mean milk Lf concentration (log) in the latter lactational period tended to be higher than those in the peak and middle periods. Milk Lf concentrations (log) tended to be proportional to the level of the somatic cell count (SCC) score. Mean milk Lf concentrations (log) in subclinical mastitic cows infected with Staphylococcus aureus and with other streptococci species were significantly (p<0.01) higher than those in cows infected with coagulase-negative staphylococci and with Corynebacterium bovis.  相似文献   

9.
This study was conducted in order to evaluate effects on prolactin (PRL) concentration and mammary milk secretion of an injectable cabergoline formulation administered to five lactating Beagle bitches during early postpartum (PP). Bitches were bled twice daily (from PP day 3 to PP day 12) and then daily (from PP day 13 to PP day 16) to assay serum PRL. On PP day 6, a subcutaneous (SC) injection of 0.1 ml/kg of placebo was administered. On PP day 9, a SC 0.1 ml/kg dose of injectable cabergoline was administered. All bitches were checked for milk production, using a clinical scoring in order to quantify milk expression from each teat. A circadian variation of serum PRL was evident during the 6 days of pre-treatment monitoring. The day after cabergoline injection, an 80% decrease of PRL serum concentration was observed (p < 0.05). The circadian oscillatory pattern of PRL secretion disappeared after administration of cabergoline, and PRL values remained significantly lower than in the previous days for the first 60 h following treatment (p < 0.001). Milk production was drastically reduced when comparing pre-treatment to post-treatment scores (p < 0.001). A single dose of injectable cabergoline caused a significant reduction in serum PRL concentration and a significant reduction in milk flow. The injectable formulation of cabergoline appeared to be safe and well tolerated.  相似文献   

10.
Elevated post‐partum progesterone metabolite (P4‐met) levels have been recently postulated to occur in lactating lynxes. The aims of this study were to monitor reproductive features in female ferrets, changes in the faecal P4‐met concentrations throughout the breeding season and ovarian activity in post‐partum lactating and non‐lactating (NL) female ferrets. Our results indicate that coinciding with the results described in the lynx, elevated faecal P4‐met concentrations occur in lactating ferrets, furthermore, that the duration of elevated secretion of P4 seems to be dependant on the duration of lactation (P4‐met at delivery, n = 47: <500 ng/g; 5–7 days after delivery, during lactation, n = 47: ≥500–800 ng/g; in females weaned at delivery, n = 4: baseline levels). Three days after ovariohysterectomy of lactating females, P4‐met concentrations decreased to baseline levels. In lactating females, the ovarian stroma is more active than that in NL ones implicating that the ovary is at least in part responsible for the elevated P4‐met concentrations. Ovaries of lactating females contained many luteinized cells either as luteinized granulose cells in the wall of late pre‐antral/early antral follicles or as corpus luteum (CL)‐like structures. Early resumption of the entire ovarian activity (developed follicles and oestrus) occurs in NL post‐partum females, while final follicular development is blocked (follicles stalls at antral stage) in the lactating ones (however, occasionally lactational oestrus may occur). We suppose that the elevated faecal P4‐met during lactation together with suckling and other hormonal effects may contribute to prevention of early returning to oestrus in nursing female ferrets.  相似文献   

11.
Most dairy ruminants are still lactating during early pregnancy, which could induce hormonal adaptations different from those observed during pregnancy alone. The incidence of concomitant lactation and pregnancy on plasma leptin has not been studied, and physiological factors involved in its regulation have not been addressed in goats. We assayed leptinemia throughout the pregnancy-lactation cycle in nulliparous and primiparous goats, starting 165 days prior to parturition and finishing 59 days after. During the first half of pregnancy, primiparous goats were lactating. Lactating non-pregnant primiparous goats were studied in parallel. Plasma leptin increased (+49%) up to mid-pregnancy in nulliparous, but not in primiparous goats. Furthermore, leptinemia was similar between pregnant and non-pregnant lactating primiparous goats, suggesting a strong leptinemia down-regulation by late lactation. Plasma leptin decreased from mid-pregnancy to parturition, more markedly in nulliparous goats, and remained depressed during early lactation at a similar level in both female types. It was lower, at 130 days of pregnancy, in goats carrying two fetuses. The leptinemia down-regulation by late pregnancy was highlighted by the lack of plasma leptin increase after drying-off late-pregnant primiparous goats, while it strongly increased in non-pregnant goats. The observation of leptinemia increase only in nulliparous goats suggests that it is not an essential endocrine adaptation during early pregnancy. By contrast, in both female types, the low leptinemia during transition from late pregnancy to lactation, and during late lactation, may be important for the adaptations that occur during lactation such as the partitioning of energy and nutrients towards essential functions and/or hyperphagia.  相似文献   

12.
为了对比不同泌乳阶段奶牛的采食、反刍、逍遥行为和日粮营养物质表观消化率,选择体重、胎次相近的8头健康荷斯坦泌乳奶牛,其中,4头处于泌乳盛期(泌乳盛期组),4头处于泌乳后期(泌乳后期组),进行了采食行为观察和消化试验,两组奶牛预饲期20 d,正试期15 d。结果表明,泌乳盛期组奶牛每天的干物质采食量为21.2 kg,极显著高于泌乳后期组的14.1 kg(P<0.01),但两组奶牛的采食时间和采食次数无显著差异(P>0.05),泌乳盛期组奶牛每千克DM采食的时间也极显著低于泌乳后期组奶牛(P<0.01);一昼夜总反刍时间泌乳盛期组奶牛极显著低于泌乳后期组(P<0.01),每千克DMI需反刍时间泌乳盛期组奶牛极显著低于泌乳后期组(P<0.01)。泌乳盛期组奶牛躺卧、走动和站立时间与泌乳后期组奶牛相比无显著差异(P>0.05)。泌乳盛期组奶牛日粮中NDF和ADF的表观消化率极显著低于泌乳后期组(P<0.01),两组奶牛NDF的表观消化率分别为55.8%、67.8%,ADF的表观消化率分别为44.2%、60.7%。  相似文献   

13.
选择泌乳期和静止期大鼠，经乳腺局部（乳导管）或外周（跖部皮下）注射辣根过氧化物酶（HRP）后，应用改良甲苯胺蓝染色法（MTB）对乳腺及其引流（鼠蹊部）和非引流（腘）淋巴结内的肥大细胞数量变化进行了研究，用荧光测定改良法检测了乳腺组织中组胺含量。结果显示，对照组乳腺及引流和非引流淋巴结内的肥大细胞数量均为静止期显著多于泌乳期（P〈0．01），乳腺中组胺含量也呈相同的变化趋势。在泌乳期从不同部位引人HRP后，乳腺及乳腺引流淋巴结内肥大细胞数量明显增多，与对照组相比差异极显著（P〈0．01）；而乳腺非引流淋巴结内肥大细胞数量则略有减少，与对照组相比差异不显著（P〉0．05）。在静止期从不同部位引人HRP后，乳腺及乳腺非引流淋巴结内肥大细胞数均减少，而乳腺引流淋巴结内的肥大细胞数则随引人部位不同而有差别：乳腺引人时增加，而外周引入时减少。本研究结果表明肥大细胞数量随生理周期的改变而增减的趋势与乳腺的发育、乳腺局部免疫状态及内分泌调节都有关系，而且不同泌乳阶段乳腺内肥大细胞对异物的易感性也存在差异。  相似文献   

14.
Suckling both, or only one contralateral mammary gland during 15 days postpartum was utilized to study lactogenic hormone binding to mammary microsomal membranes and quantitative mammary morphology in ewes. Binding of radiolabeled human growth hormone was specific for lactogenic hormones. Non-radiolabeled human growth hormone, ovine and bovine prolactin and human placental lactogen effectively competed with radiolabeled human growth hormone for binding sites but ovine and bovine growth hormone were completely ineffective. Specific binding of radiolabeled human growth hormone to 600 μg of membrane protein averaged 23 ± 3% in all lactating glands. Neither days postpartum nor treatment of contralateral mammary glands substantially altered hormone binding in lactating glands. Specific human growth hormone binding (6 ± 0.5%) in non-suckled glands (15 days postpartum both udder halves) was significantly lower (P<0.01) than in lactating tissue but only a moderate and variable reduction in specific binding was measured in membranes from glands non-suckled for 15 days but contralateral to a suckled gland (14 ± 4%). Specific binding was approximately doubled in assays with 600 compared with 300 μg of membrane protein and the pattern of binding among variously suckled glands was not changed by treatment of membranes with 4 M MgCl2 prior to assay. Most secretory cells from all lactating glands had rounded, basally displaced nuclei, apical fat globules, secretory vesicles and abundant densely stained basal cytoplasm (ergastoplasm). Alveolar lumenal area was maximal (50% of tissue area) and stromal tissue area was minimal. After 15 days of non-suckling (both udder halves) mammary cells were engorged with lipid, ergastoplasm was reduced and nuclei were irregularly shaped and randomly displaced compared with lactating tissue. In addition, lumenal area was reduced and stromal tissue more evident. Lack of suckling for 5 days had little apparent effect on mammary cytology. Like lactogenic hormone binding, mammary tissue morphology was only moderately altered by 15 days of non-suckling when the remaining gland was suckled. RNA concentration was lowest (2.1 ± 0.3 mg/g) in mammary tissue from ewes in which neither gland was suckled for 15 days postpartum but non-suckling interval had no significant effect when contralateral glands were suckled. DNA concentration was not significantly influenced by suckling treatments. Relative lactogenic hormone binding closely corresponded to changes in cytological and biochemical indices of secretory cell function.  相似文献   

15.
To obtain blood biochemical basic data of Japanese Black calves in Kagoshima Prefecture, Japan, blood samples were obtained from 582 clinically healthy calves on 27 farms. Calves were divided into three stages: the suckling stage (between 14 and 90 days of age, n=191), the early growing stage (between 91 and 180 days of age, n=200) and the late growing stage (between 181 and 270 days of age, n=191). The mean concentration of total cholesterol, triglyceride, nonesterified fatty acids, calcium and zinc, and the mean activities of γ-glutamyltransferase and alkaine phospatase in the suckling stage were significantly higher than those in the early and late growing stages (P<0.01). The mean concentration of total protein, albumin and globulin increased gradually with growing. The mean concentration of beta-hydroxybutyrate in the suckling stage was below 150 µmol/l, however, it elevated above 400 µmol/l in the early and late growing stages. The mean concentration of copper concentration was above 70 µg/dl in all stages. The mean concentration of zinc was between 90 and 110 µg/dl in all stages. These results suggest that the blood biochemical values of Japanese Black calves vary with growing stages, and the blood parameters obtained in this study are considered useful as indices for health management of Japanese Black calves.  相似文献   

16.
The aim of the present study was to determine the differences in corpus luteum (CL) functionality between the first postpartum estrous cycle and the following cycle in lactating dairy cows. Luteal blood flow (LBF), luteal size and blood progesterone (P4) concentration were monitored during the first and second postpartum estrous cycle. During the first and second postpartum estrous cycle, the mean LBF value increased (p < .05) from early to late dioestrus, while it decreased rapidly in proestrus, resulting statistically lower (p < .05) than those registered in all previous phases. Statistically significant differences were not observed between overall LBF during first and second postpartum estrous cycle (p > .05). During the first postpartum estrous cycle, P4 blood concentrations showed a significant reduction (p < .05) from dioestrus to proestrus. A different trend of P4 concentrations was observed during the second postpartum estrous cycle, where mean P4 value registered in proestrus resulted statistically lower than those registered in the previous cycle phases (p < .05). The mean P4 concentration registered over the first postpartum estrous cycle resulted statistically lower (p < .05) than that registered during the second one. A significant correlation between P4 concentrations and LBF was registered only during the second postpartum estrous cycle. Results indicate that during the first postpartum estrous cycle, P4 concentration was independent of luteal blood flow and luteal size.  相似文献   

17.
本试验旨在研究饲粮中添加不同剂量的复合酶制剂对泌乳奶牛瘤胃发酵、营养物质表观消化率、血清指标及生产性能的影响。选择体重、胎次[(2.30±0.06)胎]、产奶量[(37.00±0.03)kg/d]、泌乳天数[(90±0.15)d]相近的9头泌乳期荷斯坦奶牛,随机分成3组,每组3头。采用3×3拉丁方设计,对照组饲喂基础饲粮,试验组Ⅰ和试验组Ⅱ分别在基础饲粮基础上添加10和20 g/(头·d)的复合酶制剂,进行3期动物试验。每期21 d,其中14 d为预试期,7 d为采样期。结果表明:1)饲粮中添加10 g/(头·d)的复合酶制剂显著提高奶牛瘤胃液中丁酸的浓度(P0.05),而对总挥发性脂肪酸和其他挥发性脂肪酸的浓度无显著影响(P0.05);2)与对照组相比,饲粮中添加10 g/(头·d)的复合酶制剂显著提高奶牛对干物质、粗蛋白质和中性洗涤纤维的表观消化率(P0.05),对酸性洗涤纤维的表观消化率也有一定的提高作用(P=0.06);3)饲粮中添加10和20 g/(头·d)的复合酶制剂显著提高了奶牛的产奶量(P0.05),且10 g/(头·d)组的4%校正乳(4%FCM)产量、乳脂产量和乳糖产量分别较对照组提高4.85(P0.05)、0.49(P0.05)和0.32 kg/d(P0.05);4)复合酶制剂的添加未对奶牛血清指标产生显著影响(P0.05)。综上,饲粮中添加10 g/(头·d)复合酶制剂能够显著提高泌乳奶牛的产奶量、乳脂产量和瘤胃液丁酸浓度,且饲喂效果优于添加量为20 g/(头·d)时。综合考虑,泌乳奶牛饲粮中复合酶制剂的推荐添加量为10 g/(头·d)。  相似文献   

18.
Insulin resistance during late gestation may act as a predisposing factor of ovine pregnancy toxaemia (OPT). To evaluate the insulin action on energy metabolism in ewes with different susceptibilities to OPT, intravenous glucose tolerance tests (1 mmol glucose/kg body weight) were performed in 5.6 ± 0.7 year old, slightly underfed German Blackheaded Mutton ewes [high‐risk (HR) ewes] and 2.5 year old, overnourished Finnish Landrace ewes [low‐risk (LR) ewes] during mid and late pregnancy, during early lactation and during the dry period. Plasma samples were analysed for glucose, insulin, non‐esterified fatty acids (NEFA) and β‐hydroxybutyrate (β‐HB). The glucose elimination rate and the glucose‐stimulated first‐phase insulin secretion were significantly (p < 0.05) lower in the HR, in relation to the LR group combining the data of all gestational stages. The basal rate of lipolysis was significantly increased in the HR ewes during late pregnancy, but the NEFA clearance after the glucose load was similar in both groups during all reproductive stages. Plasma β‐HB concentrations decreased only in the LR ewes after the glucose load during late pregnancy. Results indicate an insulin resistance in the HR ewes regarding the glucose utilization and the ketone body formation during late pregnancy. The insulin resistance in the HR ewes may represent one predisposing factor responsible for the susceptibility to OPT. Further scientific work is necessary to elucidate whether this insulin resistance was due to breed, age or nutritional state.  相似文献   

19.
Experiments were performed to examine the effects of long‐term treatment with recombinant bovine somatotropin (rbST) on milk plasmin–plasminogen and milk composition by one injection of 500 mg of rbST in every 14 days throughout lactation in crossbred Holstein cattle. The animals receiving rbST gave a greater milk yield and rate of blood flow to the udder during early lactation than the control group. The milk lactose concentration remained constant while the milk protein concentration increased as lactation advanced in both groups. The level of milk fat in rbST‐treated animals was significantly greater than in controls in the early lactation period (P < 0.05). The milk sodium and chloride concentrations of the rbST‐treated animals significantly decreased in early lactation as compared with the control animals. The sodium : potassium ratio of the rbST‐treated animals was significantly lower than those of control animals in the early lactation (P < 0.05) and it markedly increased in late lactation. As lactation advanced, the concentration of plasmin in the milk gradually increased, while the milk plasminogen concentration significantly increased in both groups. The plasminogen : plasmin ratio decreased in the control animals while it increased in the rbST‐treated animals as lactation advanced. These findings demonstrate that rbST is involved the activity of the plasmin–plasminogen system but is not involved in maintaining tissue integrity in the mammary gland during late lactation in crossbred dairy cattle.  相似文献   

20.
Receptors for estrogen and progesterone were measured in cytosols prepared from specimens of canine endometrium obtained at late proestrus, day 4 of estrus, day 2 of diestrus, and at 10 day intervals from days 10 through 80 of diestrus. Twenty nine adult bitches were used, with 2 to 4 dogs used at each time point. Concentrations of estradiol receptors measured in endometrial cytosols from late proestrus through day 10 of diestrus were similar (mean +/- SEM: 9.9 +/- 2.2, 10.5 +/- 1.2, 16.3 +/- 1.6, and 16.2 +/- 2.9 pmol/g of tissue at proestrus, day 4 of estrus, days 2 and 10 of diestrus, respectively). As serum concentrations of progesterone increased during early diestrus, the concentration of estradiol receptors decreased and were significantly (P less than 0.05) lower on days 30 (4.9 +/- 1.3 pmol/g of tissue) and 40 (3.7 +/- 0.6 pmol/g of tissue) of diestrus. After day 40 of diestrus, when serum concentrations of progesterone were approaching basal concentrations, the concentration of estradiol receptors increased and remained significantly (P less than 0.05) higher from days 60 to 80 of diestrus (day 60, 13.4 +/- 2.9; day 70, 15.7 +/- 1.7; day 80, 19.8 +/- 2.4 pmol/g of tissue). As observed for estrogen receptors, the concentration of endometrial receptors for progesterone also gradually increased from late proestrus (4.9 +/- 1.3 pmol/g of tissue) to day 2 of diestrus (6.4 +/- 0.3 pmol/g of tissue).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

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