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1.
对血鹦鹉红色和白色两个品系的染色体组型进行分析。采用植物血细胞凝集素和秋水仙素活体注射法,以血鹦鹉肾细胞为材料,制备染色体。结果表明,红色血鹦鹉的核型:2n=4810sm+38st NF=58白色血鹦鹉的核型:2n=4719sm+28st NF=66。  相似文献   

2.
斑鳢和月鳢的染色体组型   总被引:1,自引:0,他引:1  
以肾脏为材料,用空气干燥法制备染色体标本,对两种鳢科鱼—斑鳢和月鳢的染色体组型进行研究。斑鳢,2n=42,核型公式为2m+4sm+18st+18t,NF=48;月鳢2n=46,核型公式为2m+14sm+18st+12t,NF=62。讨论了鳢科鱼的核型与进化问题。  相似文献   

3.
广东鲂的染色体组型   总被引:3,自引:0,他引:3  
本文以肾脏为材料,用空气干操法制备染色体标本,对广东鲂的染色体组型进行研究,其2n=48,NF=88,核型公式为26m+18sm+4st.  相似文献   

4.
利用腹腔注射秋水仙素制备肾细胞染色体的方法,对斑点叉尾鮰的染色体组型进行研究。结果表明,其二倍体数目为2n=58,核型公式为2n=6m+10sm+22st+20t,染色体总臂数NF=74。  相似文献   

5.
乌苏里拟鲿的染色体组型研究   总被引:2,自引:0,他引:2  
采用体内注射PHA和秋水仙素,体内肾细胞短期培养,常规空气干燥法制备乌苏里拟鳞的染色体玻片标本.对100个中期分裂相记数统计,确定乌苏里拟鲿的染色体数为2n=52.由测得的核型参数和按Levan等提出的染色体划分标准得出:有12对中部着丝点染色体(m),9对亚中部着丝点染色体(sm),5对亚端部着丝点染色体(st).染色体臂数(NF)为94.乌苏里拟鳞组型公式为2 n=24m+18 s m+10st.  相似文献   

6.
为了解凤鲚(Coilia mystus)染色体数量和核型结构,以上海市临港近岸的1~2龄性成熟凤鲚为材料,采用鳃丝细胞进行短期离体培养方法制备染色体标本,对凤鲚染色体核型进行了研究。结果表明,凤鲚雌雄染色体数量存在差异,雌性染色体数目为2n=47,雄性染色体数目为2n=48;核型公式为2n(♀)=47 t,2n(♂)=48 t;雌性染色体臂数(NF)=47,雄性NF=48。雄性凤鲚染色体相对长度范围为(2.66±0.27)5.54±0.25,雌性凤鲚染色体相对长度范围为(2.83±0.32)5.70±0.38,雌雄凤鲚的染色体相对长度不存在显著差异(P>0.05),各相邻两对染色体之间相对长度无明显差异(P>0.05)。在凤鲚的染色体中,最长的一对染色体上存在随体,次缢痕。凤鲚存在性染色体,其性染色体类型为ZO/ZZ型。  相似文献   

7.
刘鑫  张东  林听听  周丽青 《水产学报》2020,44(6):907-914
为了解灰海马的细胞遗传学特征,便于今后开展灰海马的种质评价与鉴定、规模化人工繁育、亲缘关系研究及人工选育等工作,实验以雌雄灰海马的背鳍为材料,采用秋水仙素浸泡和常规热滴片法制备染色体标本。借助Photoshop图像软件,将同源染色体配对、拼贴,做出染色体核型图。采用Image J软件的自定义曲线测量功能,以着丝粒的中心位置为起点,顺着染色体弯曲的形态,到染色体臂末端为终点,测量线段长度,得出图片中染色体的臂长,根据相同放大倍数下标尺的测量值,换算出染色体的实际臂长。根据臂比值,将染色体进行配对、分类后,得出灰海马的染色体核型公式。结果显示,灰海马的背鳍组织可作为其染色体制备的理想材料,实验选用的染色体制备方法能获得图像清晰、形态良好的细胞分裂相。此法简单、效果好,解决了海龙科鱼类染色体制备的难题;灰海马具有22对染色体,二倍体染色体数目2n=44,雄鱼的染色体核型公式为2n=2sm+20st+22t,雌鱼的染色体核型公式为2n=1m+2sm+20st+21t,雌鱼存在性染色体异型的现象,因此灰海马的性染色体为ZW/ZZ型。  相似文献   

8.
二、三倍体乌克兰鳞鲤染色体核型分析   总被引:2,自引:0,他引:2  
以二、三倍体乌克兰鳞鲤为材料,采用植物血球凝集素(PHA)体内注射,肾组织细胞短期培养,常规空气干燥法制备染色体.其核型分析结果:二倍体乌克兰鳞鲤染色体2n=100核型公式为:26m+30sm+30st+14t,染色体臂数NF=156;三倍体乌克兰鳞鲤染色体的核型公式为3n=150=39m+45sm+45st+21t,染色体臂数NF=234,二、三倍体染色体数之比为1:1.5.二倍体核型与已报道的鲤鱼染色体核型相似.未发现性染色体.  相似文献   

9.
采用体内注射PHA和秋水仙素,体内肾细胞短期培养,常规空气干燥法制备乌苏里拟鳞的染色体玻片标本。对100个中期分裂相记数统计,确定乌苏里拟鳞的染色体数为2n=52。由测得的核型参数和按Levan等提出的染色体划分标准得出:有12对中部着丝点染色体(m),9对亚中部着丝点染色体(sm),5对亚端部着丝点染色体(st)。染色体臂数(NF)为94。乌苏里拟鳞组型公式为2n=24m+18sm+10st。  相似文献   

10.
洞庭湖土鲶染色体核型分析   总被引:1,自引:0,他引:1  
采用PHA 和秋水仙素体内注射法,研究洞庭湖土鲶的染色体核型.试验结果表明,洞庭湖土鲶染色体组由58 条组成,按着丝粒位置可分为4组,m 组有12对染色体,sm 组有8 对染色体,st 组有7 对染色体,t 组有2对染色体,每个染色体均有相应的同源染色体.洞庭湖土鲶的2n=58,NF =98,其核型公式为:2n =24m+16sm+14st+4t.  相似文献   

11.
Energy development threatens fish and wildlife resources worldwide. This study used constrained ordinations to show fish assemblage structure associated with oil and gas well densities in the Colorado River Basin, Wyoming, but well densities explained only 6.4% of assemblage structure when compared to other factors. Threshold Indicator Taxonomic ANalysis showed significant negative threshold responses by some species to small levels of development (<0.15 wells km?2), whereas positive thresholds were less distinct. Some native and imperilled species could be disproportionately affected if future oil and gas development proceeds in a manner that will impact aquatic resources. Although existing development has not substantially influenced regional fish assemblage structure, it appears to affect a subset of species. Understanding assemblage‐level responses to development can help land managers determine appropriate development levels, prioritise areas for monitoring associated with future development and identify where land protection measures may be needed to offset potential risks.  相似文献   

12.
Important operational changes that have gradually been assimilated and new approaches that are developing as part of the movement toward sustainable intensive aquaculture production systems are presented via historical, current, and future perspectives. Improved environmental and economic sustainability based on increased efficiency of production continues to be realized. As a result, aquaculture continues to reduce its carbon footprint through reduced greenhouse gas emissions. Reduced use of freshwater and land resources per unit of production, improved feed management practices as well as increased knowledge of nutrient requirements, effective feed ingredients and additives, domestication of species, and new farming practices are now being applied or evaluated. Successful expansion into culture of marine species, both off and on shore, offers the potential of substantial increases in sustainable intensive aquaculture production combined with integrative efforts to increase efficiency will principally contribute to satisfying the increasing global demand for protein and food security needs.  相似文献   

13.
A report is presented of esophagoscopy and gastroscopy in cats and dogs using flexible endoscopes. Following consideration of the basic equipment, an account is given of patient preparation and anesthesia, topography, investigation technique and normal findings. Diagnostic potential, indications and risks involved are discussed.  相似文献   

14.
Many lepisosteid populations in North America have declined and many are now threatened as a consequence of habitat loss and alteration and commercial and sport overfishing. Over the last two decades, morphological, histological and molecular studies allowed distinguishing between different phases of development and the nutritional condition of larvae. Ontogeny of the digestive enzymes of gar larvae indicated the possibility to feed them artificial feeds since early developmental stages. An in vitro digestibility system to test different feed ingredients has been used. Important characteristics of artificial diets were identified through different feeding experiments. Endocrinological studies showed the feasibility of altering larval development and the digestive capacity of larvae. Cloning of gar growth hormone opened new avenues to enhance growth in the gars. Plasmatic vitellogenin was isolated and purified, to develop a competitive enzyme‐linked immunosorbent assay, which allowed the straightforward separation of males from females to establish appropriate proportions for reproduction and also was used to evaluate hormonal protocols to induce gonad recrudescence and spawning. This review analyzes the biology, ecology and physiology of different gar species as a basis for their domestication, mass production of larvae for repopulation experiments and for the culture of commercial‐size gar.  相似文献   

15.
奶牛酮病发病机理研究进展   总被引:2,自引:0,他引:2  
结合国内外目前研究状况较为详细的综述了奶牛酮病的病因与发病机理,并分析了奶牛酮病发生的生化机制。旨在对临床实践中很好的认识诊断和群体监控奶牛酮病提供较为系统的理论依据,保证奶牛群的高产和稳产,从而创造良好的经济效益。  相似文献   

16.
李佳  吴东波 《畜禽业》2007,(4):8-11
本试验利用饲养试验方法,研究玉米-豆粕型日粮中添加植酸酶时不同磷水平和钙磷比对生长肥育猪血清指标和骨骼性能的影响。试验选用162头20kg左右的杜长大三元杂交猪进行2×3(磷水平×钙磷比)因子饲养试验,试验分2个阶段进行,前期为生长期体重在20-60kg,后期为肥育期体重为为60-100kg。结果表明:添加植酸酶日粮磷水平对血清磷含量和碱性磷酸酶的活性影响显著(P<0.05);钙磷比对血清钙和血清磷含量的影响显著(P<0.05);添加植酸酶日粮磷水平和钙磷比对生长肥育猪骨骼性能的影响不显著(P>0.05)。在添加植酸酶条件下,降低日粮磷水平不影响猪的正常生长发育。  相似文献   

17.
“十一五”以来,我国农垦加快体制机制改革、结构调整和经济增长方式的转变的步伐,参与市场竞争,努力提升企业和产品竞争力,经济社会事业发展较快,对国家的贡献提升,职工收入、生活质量明显提高和改善,示范引领作用不断增强。本文针对农垦体制机制创新进展滞缓,战略定位与发展方向不明,经济增长方式转变和结构调整缺乏资本、人才、技术的支撑,没有处理好改革、发展、稳定、民生的关系,投资匮乏,发展后劲不足,人才短缺和劳动力素质低下的现状,提出在未来农垦体制机制创新中要特别关注改革方向、目标设定与评估,改革要在现行法律法规的框架内运作,改革时机的选择,处理好改革发展稳定与民生的关系,调整好内部利益分配关系,自觉融入区域经济,与地方协同发展等问题。为了促进农垦的发展,建议国家加大对农垦体制机制创新、现代农业建设、国有农场土地资源的保护、社会保障体系的完善等财政和政策的支持力度。  相似文献   

18.
鸡皮刺螨是鸡的一种体外寄生的节肢动物,流行十分广泛,对养殖业发展有严重影响。为了解鸡皮刺螨的发病情况,对川渝两地的126个鸡场进行流行病学调查,感染率高达71%。同时,介绍了鸡皮刺螨的生活史、危害及防治措施,是防制鸡皮刺螨不可多得的资料。  相似文献   

19.
Abstract  Catch composition, relative abundance and diversity of fish catches in open access and three old fisheries closures were compared and contrasted with previous ecological studies. There was less variation in catch community composition among the fishing grounds than the closures, suggesting that fishing has homogenised catch composition. The trap survey found that some parrotfish [ Leptoscarus vaigiensis (Quoy &Gaimard), Calotomus carolinus (Valenciennes) and Scarus ghobban Forsskål] were relatively more common and that some important predators of macro-invertebrates [ Balistapus undulatus (Mungo Park) and Cheilinus chlorourus (Bloch)] were less common in the fishing grounds than closures. Unexpectedly, and in contrast to visual census results, cumulative number of species in catch surveys was higher in open access than closures sites. This may result from fishers covering more area and habitat or a reduction in the catch of competitively subordinate and rare species by aggressive, early-caught fish that can dominate bait. Comparisons of ecological visual census surveys and fisheries-dependent methods indicated that small differences in catch composition can reflect larger ecological differences and that baiting methods can underestimate biodiversity. Ecological impacts of fishing and large-scale changes in marine ecosystems must be considerable given the many fisheries-dependent assessments report modest changes.  相似文献   

20.
温度与盐度对老年低额蚤和蚤状蚤生长及生殖的影响王丹丽,李明云,杨天勇,胡章(宁波大学,315010)关键词老年低额 ,蚤状 ,温度,盐度,生长,生殖EFFECTSOFTEMPERATUREANDSALINITYONGROWTHANDREPRODUCT...  相似文献   

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