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中药促孕液对小白鼠生殖发育结构和影响 总被引：1，自引：0，他引：1
以苯甲酸雌二醇为阳性对照，生理盐水为阴性对照，观察了中药促孕液对21日龄雌性小白鼠卵巢发育、对成年去卵巢小鼠子宫内膜和阴道上皮结构以及阴道上皮脱落细胞角化率的影响。结果表明，促孕液能显著促进幼鼠卵巢发育，使其提早出现性成熟后的形态学特征；也能促进去卵巢小鼠子宫内膜和阴道上皮增生、分泌，提高阴道上皮脱落细胞的角化率，但其作用强度明显弱于雌二醇，提示促孕液具有微弱的雌激素样作用。这种作用是促孕液治疗母畜卵静止和持久黄体性不孕症的机理之一。 相似文献
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中药促孕灌注液及其主要组分药对小白鼠阴道上皮的影响比较研究 总被引：2，自引：0，他引：2
通过阴道涂片和组织切片的观察和显微测量定量分析,比较研究了中药促孕灌注液及其主要组分药红花、淫羊藿、益母草对成年去势小白鼠阴道上皮的影响。结果表明,各药均能引起小白鼠阴道上皮增生、角化,提示它们均有微弱的雌激素样作用。各药的作用强度与它们对子宫内膜的作用强度相同:复方的作用明显强于单味药;三种单味药相比,淫羊藿作用稍强,红花和益母草的作用稍弱。 相似文献
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催情助孕液对小鼠雌、孕激素水平及其受体mRNA表达的影响 总被引：2，自引：0，他引：2
分别以0.1、0.2、0.4 mL/d剂量的催情助孕液给21日龄小鼠灌服7d后,检测体质量、脏器指数、血清雌激素和孕激素水平、子宫组织中雌激素和孕激素受体基因mRNA的表达情况,并与腹腔注射雌二醇4d的小鼠进行比较.结果显示与对照组和雌二醇注射组比较,0.2 mL的催情助孕液能显著促进小鼠子宫发育,并增强雌激素水平及其受体基因表达,而对孕激素水平及其受体基因表达无显著影响.结果表明,催情助孕液对动物生殖发育与促进发情具有较好的效果. 相似文献
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“壮阳促孕散”对小鼠雌激素样作用观察 总被引：1，自引：0，他引：1
采用常规药理实验方法,探讨中药壮阳促孕散对雌性幼小鼠子宫增重及血清雌二醇含量的影响。结果表明,壮阳促孕散能使幼年小鼠子宫显著增重,血清雌二醇含量明显增高,从而证明中药壮阳促孕散具有雌激素样作用 相似文献
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《动物医学进展》2020,(8)
本试验对存在于广东云浮市境内的棕果蝠雌性生殖器官进行了大体及组织学观察。棕果蝠雌性生殖系统由卵巢、输卵管、子宫、阴道、阴道前庭和阴门组成。卵巢一对,位于两侧肾的后方,包于输卵管系膜内,肉眼不易区分。输卵管很短,分为漏斗部、壶腹部和峡部,黏膜上皮为单层柱状上皮,黏膜形成纵向黏膜褶,并具有宽阔的输卵管腺。子宫为双子宫类型,根据子宫内膜上皮类型,每侧子宫分为子宫角和子宫颈,子宫角内膜具有高柱状上皮,并形成类似于输卵管腺的子宫腺。子宫角较粗,外径在1 000μm以上,比输卵管外径大1倍。子宫颈内膜上皮为单层高柱状分泌型细胞。子宫颈内膜形成发达的纵行黏膜皱褶,但不形成宫颈腺或腺样隐窝;两个子宫颈后半部共同形成一个圆锥状子宫颈阴道部,相当发达,顶端向后,其外表面也具有一层高柱状分泌型上皮细胞。两个子宫颈管未能抵达子宫颈后端,而是分别开口于子宫颈阴道部中间段(前后向上)的腹侧面上,分别开口于阴道。阴道穹窿长而深,其最前部形成一对盲管,分别位于左右两宫颈管的外侧。阴道长而宽阔。其前端形成阴道穹窿。阴道黏膜为厚而角化的复层扁平上皮。阴道后腹侧有雌性尿道的开口。阴门呈椭圆孔状,未形成左右两个阴唇。 相似文献
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This study examined the anatomical and histological characteristics of tubular genital organs of 51 adult female red brocket deer in the wild in different reproductive stages, collected by rural hunters in the north‐eastern Peruvian Amazon. The infundibulum was characterized by a large diameter and the presence of a highly folded and ciliated epithelium, and the isthmus has a growing secretor epithelium and a thicker muscular layer. Whereas ciliated cells are more frequent in the infundibulum, epithelial secretory cells showing abundant apical secretory blebs are more frequent in the isthmus. In non‐pregnant females in luteal phase, the endometrium transforms from a proliferative to a secretory type, showing a significant proliferation of endometrial uterine glands. The red brocket deer has four large circular folds in the cervix. The epithelium of the cervix is composed primarily of secretory cells. In pregnant females, the lumen of the endocervical canal is occupied by abundant mucous secretion. All pregnant females had one embryo or fetus, with a fetal sex ratio of 54.0% females to 46.0% males. This species has a cotyledonary, syndesmochorial and partially deciduate placenta, with 6–7 dome‐shaped caruncles per female. The red brocket deer does not present a true cornification of the vaginal epithelial cells, and no vaginal epithelial pattern was determined according the reproductive state of the female. 相似文献
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Expression of estrogen receptor alpha and beta in the uterus and vagina of immature rats treated with 17-ethinyl estradiol 总被引：3，自引：0，他引：3
Kang JS Lee BJ Ahn B Kim DJ Nam SY Yun YW Nam KT Choi M Kim HS Jang DD Lee YS Yang KH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(12):1293-1297
The action of estrogen on target organs has been actively studied with the discovery of estrogen receptor (ER) beta. This study was carried out to examine the expression of ERalpha and ERbeta in the uterus and the vagina of immature Sprague-Dawley rats treated with 17-ethinyl estradiol (EE). Twenty days old rats were subcutaneously treated with EE at the doses of 0 (vehicle control), 0.03, 0.3, 1.0, 3.0, and 10.0 microg/kg/day for three consecutive days. The treatment of EE at the doses of 0.3, 1.0, 3.0 and 10.0 microg/kg/day significantly increased the weights of the uterus and vagina of rats (p<0.01) and retained fluid in the uterus of rats. At the high doses of 3.0 and 10.0 microg/kg/day, the treatment of EE caused an increase in the uterine height, hypertrophy, and a decrease in the expression of ERalpha and ERbeta in the uterine luminal and glandular epithelium. The treatment of EE at the doses of 3.0 and 10.0 microg/kg/day also caused cornification and a decrease in the expression of ERalpha and ERbeta in the vaginal epithelium. These results suggest that the EE treatment decrease the expression of ERalpha and ERbeta in the uterus and vagina of immature rats and that may be associated with the morphological changes such as increase in the uterine height, hypertrophy of the uterine epithelium, and cornification of the vagina. 相似文献
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Cherdshewasart W Kitsamai Y Malaivijitnond S 《The Journal of reproduction and development》2007,53(2):385-393
The aim of this study was to evaluate the estrogenic activity of tuberous samples of phytoestrogen-rich Pueraria mirifica collected from 25 of 76 provinces in Thailand by vaginal cornification assay. Tuberous powders were prepared and administered to ovariectomized rats for 14 consecutive days at dosages of 10, 100 and 1,000 mg/kg BW respectively, and were compared with a daily treatment with 2 mg/kg BW 17beta-estradiol (E(2)). Rats treated with 10 mg/kg BW Pueraria mirifica showed no vaginal cornification. Treatment with 100 mg/kg BW Pueraria mirifica from 13 out of 25 plant samples resulted in development of vaginal cornification. The cell count percentages of the vaginal smeared cells for the treatment with the 2 plant samples that exhibited the fastest vaginal cornification revealed large variation in their estrogenic activities. Treatment with 1,000 mg/kg BW Pueraria mirifica from all plant samples produced vaginal cornification with the mean value for the period (day) of first appearance of cornified cells being 4.08 days compared to 2 days with 2 mg/kg BW E(2). The overall appearance period (day) of cornified cells during the treatment and post-treatment period with 1,000 mg/kg BW per day Pueraria mirifica was shorter than treatment with 2 mg/kg BW E(2). The results demonstrate that the plant population shows differential estrogenic activity as evaluated by vaginal cornification assay. 相似文献
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Histological and electron microscopical examinations of the vaginal epithelium of the cow The vaginal epithelium of the cow was not uniform. Its structure depended on the place from where a sample was taken, the hormonal status of the animal, and other (individual) factors. From each of 23 cows 11 tissue samples were taken at equal distances from the entire length of the vagina. Some cows had low blood hormone values, others were under gestagen dominance and still others under the influence of estradiol. In cows with low blood hormone values, tissue samples 1 and 2 (E 1 and E 2), which corresponded to the fornix and its junction with the vagina proper, showed an epithelium of one or two layers of cylindrical cells. Tissue samples E 3 to E 5 (cranial half of the vagina) were dominated by an epithelium consisting of three layers, i. e. two layers of basal cells and one variably shaped layer of apical cells. In E 6 to E 10 (caudal half) the height of the epithelium increased from three to about ten cell layers; a stratum intermedium of variable thickness could be discerned. Tissue sample Ell (vestibule) had a non-cornified squamous epithelium of multiple layers. Cows under the influence of gestagen had an epithelium which in principle was similar to the above findings. In cows under the influence of estradiol, the rate of cell proliferation was increased, the epithelium was thicker throughout, showed an active tendency of desquamation, and that in the vestibule had the potential to become cornified. Free cells (lymphocytes and plasma cells) were found in all areas; they were more numerous in animals under the influence of gestagen. In animals under the influence of estradiol also neutrophils were encountered. PAS-positive mucosubstances containing acid groups were found in the apical cells of the cranial end of the vagina. These substances could be responsible for a typical reaction (Kuppenreaktion) in the epithelial type that featured a stratum intermedium. Apart from folds the vaginal epithelium contained genuine intraepithelial and subepithelial glands. 相似文献
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The effects of spaying and anabolic implants on the average daily weight gain of heifers on pasture 
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下载免费PDF全文 Zobell DR Goonewardene LA Bertagnolli C Ziegler K 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(12):727-730
Two studies were carried out to determine the effects of spaying and implanting on the growth of heifers and compare Ralgro and Synovex-S for spayed heifers. In the first study, 121 crossbred heifers were randomly allocated to four groups: intact and no implant; intact and progesterone and estradiol benzoate (Synovex-S); spayed and no implant; and spayed and Synovex-S. The average daily gain (ADG) and body weight (BW) data were analyzed as a 2 × 2 factorial experiment with a pasture effect which was considered as a block. Based on ADG for days 0-120, the response to implanting was 17.6% for spayed heifers and 1.8% for intact heifers. In the second study, 31 spayed heifers implanted with zeranol (Ralgro) were compared with 30 spayed heifers implanted with Synovex-S. The data were analyzed as a one-way analysis of variance. The ADG for spayed heifers on Ralgro did not differ significantly (p>0.05) from those implanted with Synovex-S over a period of 92 days, suggesting that both implants produce similar growth responses. 相似文献
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中药促孕液及其组分药对大白鼠肠系膜微循环和家兔子宫运动的影响 总被引：5，自引：0，他引：5
运用微循环研究技术和器官运动描记技术观察中药促孕液及其组分药红花、淫羊藿和益母草对大鼠肠系膜微循环和家兔子宫运动的影响。结果表明，促孕液能使大鼠肠系膜微动脉、微静脉扩张，毛细血管网交点数增加，具有显著的改善微循环的作用，其中红花和益母草起主要作用；促孕液也能缓和地促进子宫运动，其中红花起主要作用。这两种作用是促孕液治疗母畜卵巢静止和持久黄体性不孕症的部分机理。 相似文献
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A 16‐year‐old, spayed, female poodle dog was presented for evaluation of tenesmus, with the ability to attract male dogs and a willingness to mate. The dog had undergone an ovariohysterectomy when it was 7 years old. Haematological and serum biochemistry analyses demonstrated leucocytosis and a mild uraemia. A vaginal smear was predominantly made up of superficial cells, accounting for at least 80% of the cells. Abdominal ultrasonography revealed a heterogeneous multicystic mass at the caudal abdomen and two masses with anechoic areas adjacent to the caudal pole of each kidney. A midline exploratory laparotomy identified a uterine mass and residual ovaries that were surgically excised. Histopathological examination of the mass demonstrated that it was a leiomyoma. The left ovary had a cystic structure and the right ovary had a papillary cystic adenoma. Seven months after the surgery, the owner reported that the dog was clinically normal. To the authors’ knowledge, this is the first reported case of a uterine leiomyoma after an incomplete ovariohysterectomy in the dog. 相似文献
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The normal endoscopic appearance of the caudal reproductive tract of the cyclic and non-cyclic bitch: post-uterine endoscopy 总被引：1，自引：0，他引：1
F. E. F. LINDSAY 《The Journal of small animal practice》1983,24(1):1-15
Endoscopy of the caudal reproductive tract, or post-uterine endoscopy, is a method of examining the mucosal aspect of the vagina and vestibule/vulva of the live bitch. In this study the post-uterine, or caudal, reproductive tracts of forty-five bitches of pedigree and mixed ancestry were examined endoscopically. The instrumentation and method of endoscopic examination are described. The endoscopic appearance of the mucosal aspect of the vagina, vestibule and vulva was observed during anoestrus and several phases of the sexual cycle, and in the spayed bitch. Visual records made during endoscopy were compared with the exfoliative vaginal cytology and behavioural responses. The results obtained indicate that post-uterine endoscopy is a relatively rapid and accurate method of detecting the sexual status of the bitch, and compared well with the other methods used in this study. It is suggested post-uterine endoscopy also has a value in allied investigative procedures or reproductive processes. 相似文献
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【目的】 鉴于在多囊卵巢综合征(polycystic ovary syndrome，PCOS)发病机理和治疗方式上的诸多争议，以及使用人类PCOS材料进行研究的局限性，本研究通过构建PCOS小鼠模型以探究在模型构建过程中性激素水平的变化规律。【方法】 皮下注射脱氢表雄酮(dehydroepiandrosterone, DHEA)诱导昆明白雌鼠产生PCOS样临床症状，通过结晶紫染色查看小鼠性周期是否发生停滞；利用HE染色的方式确定卵巢发育状况；利用ELISA技术调查建模过程中血清睾酮和雌二醇浓度的变化规律。【结果】 使用结晶紫对小鼠阴道上皮细胞染色可准确区分小鼠各个性周期阶段；使用6 mg/100 g DHEA持续诱导20 d后，昆明白小鼠性周期循环发生了一定的停滞，且在建模过程中体重变化与芝麻油溶剂的加入呈显著相关(P < 0.05)，与DHEA处理无关；DHEA连续处理后可见卵巢中巨大囊状卵泡出现，血清睾酮水平出现显著升高(P < 0.05)；自PCOS模型构建的第5天起，血清睾酮水平显著上升(P < 0.05)，且维持到建模结束；而血清雌二醇水平出现了阶段性变化，在第10、15天均显著高于对照组(P < 0.05)，第20天时低于对照组。【结论】 改进DHEA溶解方式可成功构建昆明白小鼠PCOS模型，在建模过程中血清睾酮持续处于较高水平，而雌二醇水平呈现先增高后降低的趋势，后者在建模中期瞬时升高的病理意义需进一步研究。 相似文献