共查询到17条相似文献，搜索用时 125 毫秒
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绵羊多胎性繁殖性状直接与绵羊的排卵数有关。本文对调控绵羊排卵数的主效基因FecB基因、FecX基因的定位、表型特征、作用机理及其候选基因的研究作一综述。 相似文献
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绵羊繁殖性状候选基因的研究进展 总被引：6，自引：0，他引：6
目前，研究发现的影响绵羊繁殖性状的候选基因主要有四个，即FecB,FecX1,FecX和FecX2w，本文就这四个基因的发现，定位及其研究进展作一系统阐述。 相似文献
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文章就FecB基因、FecX基因和FecGH基因等几个高繁殖力基因在国内绵羊上的研究和应用作了简要概述，并展望了绵羊高繁殖力基因的发展前景。 相似文献
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目前,研究发现的影响绵羊繁殖性状的候选基因主要有四个,即FecB,FecXI,FecXH,和FecX2w,本文就这四个基因的发现,定位及其研究进展作一系统阐述。 相似文献
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Mutations in BMPR-IB and BMP-15 genes are associated with litter size in Small Tailed Han sheep (Ovis aries) 总被引：2，自引：0，他引：2
Chu MX Liu ZH Jiao CL He YQ Fang L Ye SC Chen GH Wang JY 《Journal of animal science》2007,85(3):598-603
The Small Tailed Han is a prolific local sheep breed in China. The bone morphogenetic protein receptor IB (BMPR-IB) gene, which affects the fecundity of Booroola Merino sheep, and the bone morphogenetic protein 15 (BMP-15) gene, which affects the fecundity of Inverdale, Hanna, Belclare, Cambridge, and Lacaune sheep, were studied as candidate genes associated with the prolificacy of Small Tailed Han sheep. Single nucleotide polymorphisms of BMPR-IB and BMP-15 genes were detected in Small Tailed Han ewes (n = 188) by PCR-RFLP. The combined effect of the 2 genes on the prolificacy of Small Tailed Han sheep was studied. The results indicated that the same FecB mutation (Q249R) occurred in the BMPR-IB gene in Small Tailed Han ewes as found in Booroola Merino ewes. The Small Tailed Han ewes with genotypes FecB(B)/FecB(B) and FecB(B)/FecB(+) had 1.40 (P < 0.01) and 1.11 (P < 0.01) more lambs, respectively, than those with genotype FecB(+)/FecB(+). The same FecX(G) mutation (Q239Ter) of the BMP-15 gene was found in Small Tailed Han ewes as in Belclare and Cambridge ewes. The Small Tailed Han ewes with the heterozygous mutant FecX(G)/FecX(+) had 0.55 (P < 0.01) more lambs than those with the wild-type FecX(+)/FecX(+). The Small Tailed Han ewes carrying mutations in both BMPR-IB and BMP-15 genes had greater litter size than those with either mutation alone. In view of our results, marker-assisted selection using both BMPR-IB and BMP-15 genes is warranted to increase litter size in sheep and will be of considerable economic value to sheep producers. 相似文献
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采用聚合酶链式反应—单链构象多态性分析技术（polymerase chain reaction-single strand conformation polymorphism, PCR-SSCP）技术研究绵羊高繁殖力候选基因FecXG位点在多浪羊中的单核苷酸多态性。试验结果表明：多浪羊中BMP 15基因的FecXG位点并不存在多态性,推断FecXG位点与多浪羊多胎性状无关。 相似文献
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Lahoz B Alabart JL Jurado JJ Calvo JH Martínez-Royo A Fantova E Folch J 《Journal of animal science》2011,89(11):3522-3530
A new mutation in the bone morphogenetic protein 15 (BMP15) gene (FecX(R) allele) causing increased prolificacy in heterozygous (R+) and sterility in homozygous ewes has been recently described in Rasa Aragonesa, a low-prolificacy Mediterranean breed. The current study determined, first, the effect of this polymorphism on natural and eCG-induced ovulation rate (OR) and the effect of eCG dose on reproductive performance; and second, its effect on prolificacy and its interaction with progestagen + eCG treatment on farms, which have not been reported to date. The FecX(R) allele increased OR by 0.44 and 0.63 ovulations in young (n = 91) and adult (n = 84) R+ ewes, respectively (both, P < 0.01), increments less than reported in prolific breeds carrying other mutations in BMP15. When the standard dose of eCG used on farms (480 IU) was applied to R+ ewes (n = 36), an extremely high OR (3.95) was recorded, which was accompanied by greater partial failure of multiple ovulations (PFMO). On the contrary, OR using 240 IU in R+ ewes (2.90; n = 35) was similar to 480 IU in wildtype (++) ewes (2.82; n = 48; both P < 0.01 when compared with 480 IU in R+ ewes). No differences were found in the birth weight of the offspring between R+ and ++ eCG-stimulated ewes within the same litter size. To validate the genealogy identification on farms, PCR genotyping was carried out in 1,667 ewes from 4 elite flocks, resulting in a negligible misclassification of R+ ewes, which demonstrated that identification by genealogy is a reliable tool to identify FecX(R) ewes within the breeding program. In recorded farms, the natural litter size of ++ ewes (1.34, n = 599,160 lambing records) was increased due to the FecX(R) allele by 0.35 lambs (P < 0.0001, n = 6,593 lambing records). A similar increase (0.30) was observed when comparing ++ and R+ ewes treated with 480 IU of eCG (P < 0.0001, n = 62,055 and n = 866, respectively). When applying 480 IU of eCG to R+ ewes, the increase in prolificacy was only due to increased percentages of triplets (P < 0.001) and quadruplets (P < 0.0001), but not of twin births. In conclusion, the favorable reproductive performance of R+ ewes, with 0.63 extra ovulations and 0.35 extra lambs per lambing ewe, is responsible for the increased interest in the use of this polymorphism. Nevertheless, care must be taken in the application of eCG to R+ ewes, with the current results showing that the standard dose increases prolificacy by only increasing triple and higher-order births. 相似文献
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为研究耐药相关基因在肉鸡养殖场中的分布流行情况，本研究以河北省肉鸡养殖场待出栏肉鸡为研究对象，采用随机抽样方式采集肉鸡泄殖腔拭子样品264份，粪便污染地面样品9份，进行blaNDM、blaOXA、blaCTX-M、armA、fexA、cfr、mcr-1、qnrS 8种耐药基因和int1、ISCR1 2种与耐药基因散布密切相关基因的实时荧光定量PCR检测。结果显示，10种基因在273份样品中均有不同程度检出，检出率在4.03%~97.07%之间，检出率最低的为armA基因，最高的为int1基因；样品中有基因共存情况，并以3种耐药基因共存的情况最多，有4份样品存在1种耐药基因，6份样品未检出任何耐药基因，没有样品共存8种耐药基因；通过数据分析，共得到41种不同的耐药基因谱型，其中常见的耐药基因谱型为blaCTX-M-fexA-mcr-1和blaOXA-blaCTX-M-fexA-cfr-mcr-1-qnrS；分析发现样品携带两种及以上耐药基因时，与耐药基因散布相关的int1和ISCR1元件检出率越高，耐药基因在样品中检出的种类越多；耐药基因blaOXA、blaCTX-M、fexA、cfr、mcr-1、qnrS与int1基因的共存有统计学意义（P<0.05）；耐药基因blaNDM、blaOXA、blaCTX-M、fexA、cfr、qnrS与ISCR1元件的共存有统计学意义（P<0.05）。本研究为畜牧兽医养殖行业耐药性监测工作提供了基于样品的新型实时荧光定量PCR检测方法，为有效预防耐药菌、耐药基因产生、控制耐药性的快速传播提供了可靠的数据支持。 相似文献
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【目的】研究广东省茂名地区屠宰环节猪肉中携带的沙门氏菌的耐药性和毒力特征,为该地区食源性沙门氏菌的危害评估和防控措施制定提供依据。【方法】从广东省茂名地区屠宰场采集的猪肉、脾脏、肝脏样本中分离到19株沙门氏菌,采用K-B药敏纸片法检测其对β-内酰胺类、氟喹诺酮类、氨基糖苷类、四环素类、酰胺醇类和磺胺类抗菌药物的耐药性,用PCR方法检测β-内酰胺类耐药基因(blaTEM、blaOXA-1、blaSHV)、氟喹诺酮类耐药基因(qnrA、qnrS、qnrB)、氨基糖苷类耐药基因(aadA1、aac(6')-Ⅰb、rmtB)、四环素类耐药基因(tetA、tetB、tetC)、酰胺醇类耐药基因(Cat1、floR)、磺胺类耐药基因(SulⅠ、SulⅡ、SulⅢ)和10种毒力基因(mogA、sseL、mgtC、bcfA、araB、spvR、spvA、spvB、spvC、spvD)的携带情况。【结果】19株沙门氏菌耐药严重,对四环素、多西环素、氯霉素、氟苯尼考、磺胺异噁唑的耐药率均＞50％,对四环素、多西环素和氯霉素的耐药率最高,均为89.5%(17/19),对3种及其以上抗菌药物耐药率为89.5%,最高对11种抗菌药物均有耐药性;blaTEM、qnrA、aadA1、aac(6')-Ⅰb、Cat1、floR、SulⅠ、SulⅢ耐药基因的检出率较高(≥50.0%),blaTEM耐药基因检出率最高为84.2％,同时携带5个及以上耐药基因的菌株占73.7%,最高携带12个耐药基因;mogA、mgtC、bcfA、araB毒力基因均有检出,且检出率均在70％以上,其中mogA基因的检出率高达100%,其余6种毒力基因均未检出。【结论】广东省茂名地区屠宰场猪肉中沙门氏菌多重耐药严重,携带多种耐药基因且具有复杂的基因组合类型,同时携带多种毒力基因。 相似文献
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旨在探讨初次卵裂时间对猪孤雌胚胎发育潜能及其基因相对表达水平的影响。本试验从健康母猪卵巢上抽取卵母细胞进行体外成熟培养，将猪孤雌激活胚胎分为早期卵裂组（16~22 h）与晚期卵裂组（26~32 h）统计比较卵裂率和囊胚率，并对囊胚的多能性相关基因Oct4、Sox2、Klf4等和凋亡相关基因Bcl-xl、Bax、Caspase-3的相对表达水平进行分析检测。结果表明，猪孤雌激活胚胎在16~22 h发生卵裂的为50%~60%，而26 h之后发生卵裂的不到20%，在18 h前完成第一次卵裂的胚胎囊胚发育率为79%，42 h后发生初次卵裂的胚胎无法发育至囊胚期。猪孤雌激活胚胎早期卵裂组的囊胚发育率显著高于晚期卵裂组（P<0.05）。早期卵裂组囊胚的Oct4、Nanog、Sox2、Klf4基因的表达量显著高于晚期卵裂组（P<0.05），Oct4、Sox2、Klf4基因的相对表达量极显著高于晚期卵裂组（P<0.01），Bax和Caspase-3基因的相对表达水平极显著低于晚期卵裂组（P<0.01），而Bcl-xl作为保护因子其表达量相对于晚期卵裂胚胎（26~32 h）显著上调（P<0.05）。结果显示，初次卵裂时间较早的猪孤雌激活胚胎发育潜能显著高于较晚卵裂胚胎，其囊胚多能性相关基因表达上调，凋亡相关基因表达下调，卵裂时间可作为鉴定猪孤雌激活胚胎发育潜力的重要参数。 相似文献