共查询到19条相似文献，搜索用时 593 毫秒
1.
用毛细管气相色谱法测定饲料添加剂维生素E原料中维生素E含量.以正三十二烷为内标物,采用DB-1毛细管柱(30 m×0.25 mm×0.25um),FID检测器;氮气为载气;柱温为280～290℃,检测器温度为290～300℃;进样口温度为290-300℃;无分流进样.在此色谱条件下,维生素E和内标物正三十二烷分离良好,在0.994～4.013 mg/mL浓度范围内呈良好线性关系,r=0.9998,经方法评估函数的评价表明,方法不存在恒定系统误差和相关性系统误差,方法准确性较好.本法操作简便易行,系统适用性良好,适用于饲料添加剂维生素E原料中维生素E含量测定. 相似文献
2.
高效液相色谱法测定维生素E粉含量 总被引：1，自引：0，他引：1
用高效液相色谱法测定饲料添加剂维生素E粉中维生素E含量。采用EclipseXDBC18柱(150mm×4.6mm,5μm),以甲醇-水(98∶2,V∶V)为流动相,流速为1.2mL/min,检测波长为285nm,柱温为25℃。在此色谱条件下,维生素E在0.104￣0.602mg/mL浓度范围内呈良好线性关系,r=0.9998,平均回收率为100.3%,RSD为0.72%。本法操作简便易行,系统适用性良好,适用于饲料添加剂维生素E粉中维生素E含量测定。 相似文献
3.
天然维生素E因具有促进动物生长、提高肉品质等功能,已被逐步用于宠物及畜禽饲养,但行业中尚无规范该类饲料及饲料添加剂产品的标准及检测方法。本研究旨在建立采用正相液相色谱-紫外检测器(NPLC-UV)测定饲料添加剂中8种天然维生素E含量的分析方法。样品经甲醇浸润后,以乙酸乙酯/正己烷(3∶97,V/V)为提取溶剂在室温下进行超声浸提。使用Kromasil 60-5 diol(4.6 mm×150 mm)色谱柱进行分离,正庚烷/四氢呋喃(100∶4,V/V)等度洗脱,流速1.0 mL/min,柱温40℃,紫外检测波长为292 nm。结果表明:生育酚和生育三烯酚含量在0.2～50.0μg/mL时线性关系良好(R2≥0.999 0),方法定量限为0.01%,加样平均回收率为81.3%～109.8%,相对标准偏差(RSD)小于7.9%。通过对实际样品测定,该方法操作简单,结果准确可靠,有助于规范天然维生素E饲料产品市场。 相似文献
4.
为测定饲料添加剂原料中D-生物素含量,建立并优化了高效液相色谱法(HPLC),采用色谱柱为Agilent XDB-C_(18)反相柱(150 mm×4.6 mm,5μm),流动相为乙腈+缓冲液(含0.1%高氯酸钠和0.1%磷酸的水溶液)=8.5+91.5(体积比),进样量为20μL,检测波长为210 nm,流速为1.2 mL/min。D-生物素在25～400μg/mL范围内线性关系良好,相关系数为1.00000,检出限1.5 mg/kg,方法回收率为99.5%,RSD为0.5%(n=9)。本方法快速简便,能够准确测定饲料添加剂原料中D-生物素的含量,相对于酸碱滴定法有更好的专属性。 相似文献
5.
用示差折光-高效液相色谱法测定天然甜菜碱含量。采用Na+形式阳离子交换柱(300mm×7.8mm),以1mmol/l硫酸钠溶液为流动相,流速为0.6ml/min,柱温为(75±2)℃,示差折光检测器内控温度为40℃,在此色谱条件下,甜菜碱在2.005～10.026mg/ml浓度范围内呈良好的线性关系(r=0.9998),方法平均回收率为98.8%(n=9,RSD=0.41%)。本法操作简便易行,系统适用性良好,适用于饲料添加剂天然甜菜碱含量测定。 相似文献
6.
7.
8.
研究制定高效液相色谱法测定饲料添加剂中盐酸林可霉素含量的方法。采用waters2695高效液相色谱仪,C18色谱柱(150 mm×4.6 mm,5μm);以0.05 mol/l硼砂溶液:甲醇(55:45)为流动相;流速:1.0 ml/min;检测波长:214 nm。主峰和相邻峰有较好的分离;盐酸林可霉素在14.7~492μg/ml范围内线形良好,R2=0.998 7(n=6),检测限为0.15μg,平均回收率分别为98.7%、99.5%、99.1%。该方法快速、灵敏、可靠,可用于饲料添加剂中盐酸林可霉素的含量测定。 相似文献
9.
用HPLC法测定混合型饲料添加剂中L-苹果酸(malic acid,LMA)的含量。采用C18(250 mm×4.6 mm×5μm)色谱柱,以0.02 mol/l磷酸二氢钾:乙腈=982(v:v),流速0.8 ml/min,柱温25℃,检测波长210 nm,进样量10μl。结果表明:L-苹果酸对照品和样品的保留时间均是3.9 min,其线性方程分别为Y=0.390 9X-4.578 6,R=0.999 8(n=5),在126.25~2 020μg/ml的范围内,线性关系良好,其最低检测量为12.23μg/ml。精密度的RSD为0.43%,表明此方法精密度较好;重现性的RSD为0.17%,表明重现性良好;回收率的RSD为0.61%。用此方法检测出其混合饲料添加剂中L-苹果酸含量为(99.01±0.11)%,符合产品规定的质量要求。该实验建立的HPLC方法可快速、准确测定混合饲料中L-苹果酸含量,为其质量控制提供科学依据。 相似文献
10.
反相高效液相色谱法测定饲料中喹乙醇的含量 总被引：2，自引：0，他引：2
建立了反相高效液相色谱法测定饲料级喹乙醇、预混合饲料和配合饲料中喹乙醇含量的方法。色谱柱为C1 8,1 5 0× 4.6mm ,5 μm ;流动相为甲醇 +水 ( 1 5∶85 ,V/V) ;检测波长为 2 60nm ;柱温为室温。结果表明 :在 0～ 1 83.2 6μg/mL之间呈现良好的线性关系 ,相关系数r2 =1 .0 ;3个不同浓度样品加样回收率分别为 96.1 4%、98.61 %和 99.42 %。本方法简便 ,快速 ,准确度和精密度较高 ,适用于饲料中喹乙醇含量的测定 相似文献
11.
D C Van Metre R J Callan 《Veterinary Clinics of North America: Food Animal Practice》2001,17(2):373-402, vii-viii
12.
13.
14.
Jewell DE Yu S Joshi DK 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(3):235-243
Skin problems are common in small animal practice. Oxidative stress, or the imbalance between prooxidants and the body's antioxidant defense system, likely plays a role in the development of skin disease. According to this study, increasing amounts of vitamin E in foods for dogs and cats increases serum and cutaneous concentrations of vitamin E. Based on available scientific data, these increases in vitamin E concentration are likely to be beneficial. However, the relationship between increases in serum and skin vitamin E concentrations and the prevention, development, and treatment of skin disease remains to be elucidated by intervention studies. 相似文献
15.
Effect of vitamin E and copper on the vitamin E status and performance of growing pigs 总被引：5，自引：0，他引：5
A 13-wk trial was conducted with 32 pigs to determine the effects of dietary Cu (250 ppm) and alpha-tocopheryl acetate (ATA, 22 IU/kg) on the performance, serum enzymes, serum and tissue tocopherols, and antibody production in growing pigs. Pigs were fed corn-soybean meal diets containing 21% CP the first 4 wk and 18% CP during the rest of the trial. All feed was stored a minimum of 14 d before it was fed. The addition of Cu decreased (P less than .01) the concentration of alpha-tocopherol in the feed. alpha-Tocopherol concentrations were less than .01 mg/kg in the starter diet and less than 2 mg/kg in the grower diet after 14 d of storage. Supplemental Cu or ATA had no effect on ADG, feed intake, or gain:feed during the first 4 wk. During wk 5 to 13, the addition of Cu to diets containing no ATA increased daily feed intake and decreased gain:feed, but with ATA addition, feed intake decreased and gain:feed increased, resulting in a Cu x ATA interaction (P less than .05). The addition of Cu or ATA had no effect (P greater than .1) on serum glutathione peroxidase or lactic dehydrogenase activity. Serum tocopherols were reduced (P less than .05) by the addition of Cu during wk 1 to 4, 6 (P less than .01), and 7 (P less than .05) and increased (P less than .01) by ATA addition during the entire experiment. The addition of ATA increased the tocopherol concentrations in bile, ham, heart, pancreas, kidney, spleen, liver, psoas and longissimus muscle (P less than .01), kidney fat, backfat, and adrenal gland (P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
16.
Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E. 相似文献
17.
Background
Increasing the polyunsaturated fatty acid (PUFA) content and decreasing the saturated fatty acid (SFA) content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid (FA) composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep.Methods
Forty-two male Aohan fine-wool lambs (5 mo old) with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 (control group), 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the longissimus lumborum (LL) and gluteus medius (GM) muscles.Results
Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation (P < 0.05). However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA (MUFA) content in the LL and GM (P < 0.05). All six doses of vitamin E significantly increased cis9 trans11-conjugated linoleic acid (c9t11-CLA) content in the LL compared with the control group (P < 0.05).Conclusions
Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9t11-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E. 相似文献18.
M. S. Awawdeh A. Q. Talafha 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(3-4):176-182
AbstractThis study investigated the effects of vitamin E and selenium (Se) injections on concentrations of vitamin E, vitamin A, and Se in blood and milk to predict when injections may improve ewes’ performance. Awassi ewes received 0 (control, 13 ewes) or 15 plus 0.05?mg/kg BW of vitamin E and Se (injected, 15 ewes) at 1 and 4 weeks postpartum. Blood and milk samples were collected weekly for 6 weeks. Injections increased vitamin E, decreased Se, and had no effects on vitamin A levels in blood. Blood vitamin E was correlated positively with vitamin A. Injections increased vitamin E, but had no effects on vitamin A or Se levels in milk. Milk vitamin A was positively correlated with vitamin E and Se levels in the control group. Milk vitamins E and A were positively correlated with their corresponding levels in blood. Milk somatic cell count was negatively correlated with blood vitamin A levels. 相似文献
19.
目前我国对自然资源、生态环境保护意识日趋增强 ,对高品质羊肉产品需求的日益增加 ,规模化舍饲养羊正在得到迅速发展 ,许多羊场尤其是农区的羊场多以玉米秸、小麦秸等农作物秸秆作为羊饲料中主要的粗饲料 ,维生素E营养不能满足羊的需要的可能性加大。本文就饲料中补充维生素E对羊生理功能、繁殖性能和生产性能的影响等方面进行综述。1 维生素E及其制剂维生素E(又称生育酚 )是一组化学结构近似的酚类化合物 ,自然界中存在α、β、γ、δ等 8种具有维生素E活性的生育酚 ,其中D -α -生育酚活性最高。α -生育酚具有旋光性 ,在空气中易氧… 相似文献