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1.
猪α-（1,2）岩藻糖转移酶2（FUT2）基因为鞘糖脂生物合成-球系列通路中的重要基因,可能在断奶仔猪抵抗大肠杆菌F18侵染过程中发挥着调控作用。为探究猪FUT2基因的序列结构及其生物学功能,试验采用PCR扩增得到地方猪品种东串猪FUT2基因的CDS全序列,进而预测和分析FUT2基因的蛋白质序列及其功能区域,同时对其在8头35日龄东串断奶仔猪11个组织中的表达水平进行检测与分析。结果显示,FUT2基因的CDS序列全长为1 023 bp,共编码340个氨基酸,FUT2蛋白为脂溶性的亲水蛋白,蛋白结构不稳定,该蛋白存在1个跨膜螺旋结构,但不存在信号肽,表明FUT2蛋白为膜蛋白;FUT2蛋白存在2个N-糖基化位点（185和305位氨基酸）,无O-糖基化位点,此外该蛋白还存在14个潜在的磷酸化位点,包括6个Ser、2个Thr和6个Tyr,对其功能区域进行分析发现,FUT2蛋白存在1个超级家族保守结构域：FUT1-FUT2-like（58-319位氨基酸）;系统进化树结果显示,猪与牛的亲缘关系相对较近,与人、黑猩猩、大鼠和小鼠等亲缘关系相对较远;FUT2基因在东串断奶仔猪11个组织中均有表达,在消化道和免疫组织中表达水平较高。试验结果推测FUT2基因在断奶仔猪抵抗大肠杆菌F18中可能具有一定的作用,且可能是通过合成岩藻糖转移酶间接发挥其抵抗大肠杆菌F18的作用。 相似文献
2.
大肠杆菌刺激和LPS诱导条件下猪小肠上皮细胞FUT1和FUT2基因表达变化 总被引：1，自引：0，他引：1
本实验运用Real-time PCR方法检测分析α-(1,2)岩藻糖转移酶1(FUT1)和α-(1,2)岩藻糖转移酶2(FUT2)基因在大肠杆菌(E.coli)F18ab、F18ac感染以及内毒素(LPS)诱导小肠上皮细胞(IPEC-J2)前后的mRNA表达差异,在细胞水平上进一步验证FUT1、FUT2基因的功能并探讨其在抗E.coli F18侵染过程中的作用机制。结果表明:FUT1、FUT2基因在E.coli感染和LPS诱导细胞后的mRNA表达水平均极显著升高(P0.01),且FUT2基因的上升趋势要高于FUT1基因。由此推测,FUT1和FUT2基因的表达水平与仔猪抵抗E.coli F18感染的能力密切相关,FUT2基因可能发挥着比FUT1基因更重要的调控作用。 相似文献
3.
为了揭示猪α-（1,2）岩藻糖转移酶2（FUT2）基因密码子使用特性并提高其在外源宿主内的表达量,本研究综合运用EMBOSS Explorer网站和CodonW软件包分析了猪FUT2基因的密码子使用偏好性的相关参数,并与3种模式生物（大肠杆菌、酵母菌和果蝇）基因组密码子使用模式进行比较,最后参照与之最为相近的模式生物基因组密码子使用方式,利用JCat和OPTIMIZER两个密码子优化网站对猪FUT2基因密码子进行优化。结果显示,猪FUT2基因表达水平不高,存在24种偏好密码子,且这24种偏好密码子中有23种以G/C结尾;猪FUT2基因与果蝇基因组密码子使用模式的差异小于大肠杆菌和酵母基因组,表明果蝇更适合猪FUT2基因的外源表达;根据果蝇基因组密码子使用模式优化猪FUT2基因密码子,优化后其适应指数有了明显提高,而整体GC含量没有明显变化,说明在理论上猪FUT2基因优化成功。本研究从翻译水平上揭示了猪FUT2基因的密码子使用特性,为其在遗传改良中选择最佳外源表达系统及提高其在宿主细胞内的表达水平提供一定的理论依据。 相似文献
4.
旨在探究杂交组合断奶仔猪腹泻与α（1，2）岩藻糖转移酶基因1（FUT1）遗传变异的关联性。本试验选择35日龄的杜滇、杜藏、杜洛克断奶仔猪为研究对象，按品种分为3组，分别为113、165、87个重复。通过测序对FUT1基因多态性进行检测，分析基因多态性与断奶仔猪腹泻率的相关性。结果发现，FUT1基因存在T18C、C229T、C2418A、A306G 4个多态性位点，多态性和杂合度均处于中度，C229T、C2418A、A306G为错义突变，可能是影响猪腹泻的关键位点。T18C位点TT基因型的杂交组合杜滇猪和杜藏猪、C229T位点的CC基因型、C2418A位点的CA基因型和A306G的GG基因型的3种猪腹泻程度相对较严重，呈显性遗传。结果说明，品种和FUT1基因多态性对断奶仔猪腹泻的影响有一定的差异性和相关性。 相似文献
5.
苏太仔猪FUT1基因M307位点多态性与F18大肠杆菌抗病相关性的体外鉴定 总被引：2，自引：0，他引：2
采用PCR-RFLP方法检测了江苏苏太断奶仔猪FUT1基因M307位点等位基因多态性分布,在所检的49头仔猪中,GG基因型个体16头,AG基因型19头,AA基因型14头。在此基础上,制备上述不同基因型个体仔猪小肠上皮细胞,分别与表达F18ab菌毛的野生型大肠杆菌、表达F18ac菌毛含fed操纵子全基因的重组大肠杆菌和V型系统表面分泌表达F18abFedF亚单位的重组大肠杆菌进行体外黏附试验和黏附抑制试验。研究结果表明:FUT1基因M307位点中GG型和AG型仔猪小肠上皮细胞均能黏附上述3种大肠杆菌,而AA型个体小肠上皮细胞则不能黏附。将上述3种大肠杆菌分别与抗F18ab菌毛高免血清、F18ac菌毛高免血清及抗F18abFedF亚单位单因子血清作用后,则失去黏附仔猪肠上皮细胞能力。上述结果对苏太猪从体外试验上证明了FUT1基因M307位点多态性与断奶仔猪腹泻和水肿病存在着直接的相关性。 相似文献
6.
Coddens A Verdonck F Tiels P Rasschaert K Goddeeris BM Cox E 《Veterinary microbiology》2007,122(3-4):332-341
F18(+)Escherichia coli have the ability to colonize the gut and cause oedema disease or post-weaning diarrhoea by adhering to specific F18 receptors (F18R) on the porcine epithelium. Although it is well established that a DNA polymorphism on base pair 307 of the FUT1 gene, encoding an alpha(1,2)fucosyltransferase, accounts for the F18R phenotype, the F18R nature is not elucidated yet. The aim of the present study was to investigate the correlation between the presence of H-2 histo-blood group antigens (HBGAs) or its derivative A-2 HBGAs on the porcine gut epithelium and F18(+)E. coli adherence. A significant positive correlation was found between expression of both the H-2 (r=0.586, P<0.01) and A-2 (r=0.775, P<0.01) HBGAs and F18(+)E. coli adherence after examination of 74 pigs aged from 0 to 23 weeks. The majority of the genetically resistant pigs (FUT1M307(A/A)) showed no HBGA expression (91.7%) and no F18(+)E. coli adherence (83.3%). In addition, it was found that F18R expression levels rise with increasing age during the first 3 weeks after birth and that F18R expression is maintained in older pigs (3-23 weeks old). Taken together, these data suggest that, apart from H-2 HBGAs, A-2 HBGAs might be involved in F18(+)E. coli adherence. 相似文献
7.
Prevalence of fimbial colonization factors F18ab and F18ac in Escherichia coli isolates from weaned piglets with edema and/or diarrhea in China 总被引：3，自引：0，他引：3
F18ab and F18ac are important fimbrial colonization factors of verotoxigenic Escherichia coli (VTEC) and/or enterotoxigenic E. coli (ETEC) in weaned piglets with edema disease and/or diarrhea. To further investigate their prevalence and correlation to pathogenic E. coli, a duplex PCR, using three primers derived from the nucleotide sequence of the F18 major fimbrial subunit gene (fedA), and a direct agglutination test, using a monoclonal antibody specific for the antigenic factor 'a' of F18, were performed. Among 60VTEC, 24VTEC/ETEC and 24 ETEC isolates tested from weaned piglets with edema disease and/or diarrhea in different pig farms in the Jiangsu Province of China, 52 isolates (48.15%) were positive in the direct agglutination test and 63 isolates (58.33%) were positive in the duplex PCR. Among 63 PCR-positive isolates, 53 isolates (49.07%) were F18ab-positive and 10 isolates (9.26%) were F18ac-positive. In addition, the F18ab gene was more frequently detected in VTEC (61.67%) or VTEC/ETEC (62.50%) than in ETEC (4.17% only), while the F18ac gene was more frequently detected in VTEC/ETEC (33.33%) than in ETEC (8.33%) or VTEC (0%). Furthermore, F18ab was more frequently associated with Shiga toxin 2e (Stx2e), whereas F18ac was more frequently associated with enterotoxin ST I. These results suggest that the duplex PCR performed in this experiment is a more reliable method for identification of F18+E. coli, and that F18 is a more important virulence factor of VTEC and VTEC/ETEC. 相似文献
8.
To investigate the association of pathogenic Escherichia coli fimbrial adhesins with the development of diarrhoea in piglets of different age groups and to test their relative competitiveness, piglets were orally inoculated with a mixture of E. coli strains harbouring F4, F5, F6, F18 and F41 fimbrial genes. A total of 537 E. coli strains with haemolytic activity were isolated from 36 diarrhoeic piglets. The F4 fimbrial gene was observed in 98.5%, 97.6% and 80.6% strains carrying fimbrial genes isolated from diarrhoeic piglets that were infected at 1, 3 and 5 weeks of age, respectively. These data demonstrate that F4 fimbriae are highly associated with diarrhoea in piglets of all age groups. Interestingly, the F18 fimbrial gene was observed in 2.4% and 25.4% strains carrying fimbrial genes isolated from the 3- and 5-week-old groups, respectively, which confirms that F18 fimbriae are associated with diarrhoea in piglets from late stages of suckling to post-weaning, and are more related to diarrhoea in weaned than in unweaned piglets. 相似文献
9.
以产志贺毒素样大肠杆菌（SLTEC）F18ab血清型标准菌株107/86基因组DNA为模板,利用PCR技术成功扩增出编码F18ab完整菌毛操纵子fed基因,克隆入表达载体pBR322,经限制性内切酶酶切分析,DNA琼脂糖电泳鉴定并结合序列测定分析,构建和筛选出含fed完整基因正确插入的pBR322-fed重组质粒,将上述重组质粒转化至不含任何菌毛结构的大肠杆菌SE5000,该表达重组菌能分别与兔抗F18ab亚单位蛋白FedF高免血清、鼠抗F18ab菌毛a单因子单克隆抗体、兔抗F18ab菌毛高免血清和抗F18ab菌毛IgG抗体产生明显的凝集反应。用热抽提法分别抽提和纯化SLTEC F18ab标准株107/86和重组菌SE5000（pBR322-fed）体外表达的F18ab菌毛,纯化菌毛经SDS-PAGE电泳和考马斯亮蓝染色获单一相对分子质量约为15 000蛋白条带。Western-blotting结果表明：兔抗F18ab菌毛高免血清能特异性识别SLTEC F18ab标准株107/86和重组菌SE5000（pBR322-fed）所提纯的单一主要结构蛋白。用重组菌SE5000（pBR322-fed）进行易感仔猪小肠上皮细胞体外黏附试验和黏附抑制试验,结果表明：重组菌SE5000（pBR322-fed）和SLTEC F18ab标准株107/86一样具有较强的黏附易感仔猪小肠上皮细胞的能力,而兔抗F18ab菌毛高免血清能有效地抑制上述重组菌SE5000（pBR322-fed）和SLTEC F18ab标准株107/86对易感仔猪小肠上皮细胞的黏附结合。 相似文献
10.
W.B. Bao S.L. Wu H.H. Musa G.Q. Zhu G.H. Chen 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2008,125(6):427-430
Escherichia coli F18 bacteria producing enterotoxins and/or shigatoxin (ETEC/STEC) are main pathogens that cause oedema disease and postweaning diarrhoea in piglets, and alpha‐1‐fucosyltransferase (FUT1) gene has been identified as a candidate gene for controlling the expression of ETEC F18 receptor. The genetic variations at nucleotide position 307 in open reading frame of FUT1 gene in one wild boar breed and 20 western commercial and Chinese native pig breeds were investigated by polymerase chain reaction–restriction fragment length polymorphism. The results showed that the genetic polymorphisms of the FUT1 locus were only detected in western pig breeds and the Chinese Taihu (including Meishan pig, Fengjing pig and Erhualian pig), Huai and Lingao pig breeds; only Duroc and Pietrain possessed the resistant AA genotype, while the wild boar and other Chinese pig breeds only presented the susceptible genotype GG. The results indicated that Chinese native pig breeds lack genetic factors providing resistance to ETEC F18 bacteria. The resistant allele to ETEC F18 might originate from European wild boar. It was inferred that oedema and postweaning diarrhoea caused by ETEC F18 have close relationship with the growth rate, which can explain why on the contrary Chinese native pig breeds have stronger resistance to oedema and postweaning diarrhoea in piglets compared with western pig breeds. 相似文献
11.
为了解不同比例低蛋白氨基酸平衡日粮对断奶仔猪生长性能及氮平衡的影响,试验将96头初始体重9.8 kg左右的杜×长×大断奶仔猪随机分成4个处理,每个处理分别饲喂含不同蛋白水平的玉米-豆粕型饲粮(20%CP、19%CP、18%CP和17%CP),通过补充合成氨基酸,使各处理组的氨基酸与要求相一致。正式试验期为28 d,测定断奶仔猪生长性能、氮平衡指标。结果表明,日粮蛋白水平降至18%时,不会影响断奶仔猪生长性能,且显著降低尿氮、粪氮和总氮排放量(P<0.01);但随着日粮蛋白水平进一步降至17%时,显著降低仔猪平均日增重(P<0.01),显著增加料重比(P<0.01);18%CP组与17%CP组总氮排放量、氮表观利用率、氮表观生物学效价差异不显著(P>0.05)。说明添加合成氨基酸满足断奶仔猪需要,将日粮蛋白水平降至18%,不仅不会影响仔猪生长性能,而且还会降低氮排放,减少环境污染。 相似文献
12.
为了获得大通牦牛DQA2基因序列,并研究其序列特征及编码蛋白的结构和功能,本研究采用DNA混合池扩增后直接测序的方法,通过DNAstar软件和在线服务器预测大通牦牛DQA2基因CDS区编码蛋白的理化性质、疏水性/亲水性、糖基化位点、信号肽、跨膜结构、二级结构以及三级结构。结果表明,大通牦牛DQA2基因CDS区长度为765bp,编码253个氨基酸。蛋白结构预测结果表明,该蛋白是一种混合型的可溶性蛋白质,具有4个潜在的N-糖基化位点,分别为Asn31、Asn96、Asn260、Asn369;在第1~23位氨基酸之间存在信号肽;存在1个典型的跨膜螺旋区,氨基酸序号为216-239;蛋白质功能显示该蛋白存在信号受体活性、催化活性、运载体、调节代谢过程、细胞定位及免疫系统过程的功能。本研究结果为进一步研究大通牦牛的遗传育种提供理论基础。 相似文献
13.
本试验旨在研究仔猪出生后10~20 d,早期断奶仔猪小肠谷氨酸转运载体基因表达情况与哺乳仔猪的差异。试验分别从40头不同母猪的仔猪中各选出体重相近,10日龄的"杜×长×大"三元杂交仔猪1头,共40头仔猪,随机不配对分为2组,每组20头仔猪,对照组(哺乳组)为哺乳仔猪,随母猪喂养;试验组(断奶组)为断奶仔猪,隔离断奶饲养;试验期10 d。饲养结束,每组随机取12只仔猪,宰杀取空肠和回肠,测定谷氨酸转运载体兴奋性氨基酸转运载体1(EAAC1)蛋白质表达情况和游离氨基酸含量。结果显示,断奶显著降低了仔猪空肠和回肠EAAC1(57和73 ku)及其相关蛋白谷氨酸转运联合蛋白(GTRAP3-18)(50 ku)的蛋白质和mRNA表达量(P0.05)。断奶提高了仔猪空肠游离谷氨酸和总氨基酸含量,却降低了仔猪回肠游离谷氨酸和总氨基酸含量,差异显著(P0.05)。结果提示,早期断奶降低EAAC1和GTRAP3-18的蛋白质含量,这可能与早期断奶仔猪遭受营养谷氨酸缺乏导致的肠道氨基酸吸收转运障碍有关。 相似文献
14.
为了探究小尾寒羊富含半胱氨酸酸性分泌蛋白类似物1（secreted protein acidic and rich in cysteine like 1,SPARCL1）基因结构及其各组织间表达差异,试验提取小尾寒羊肝脏组织总RNA,根据GenBank中公布的绵羊SPARCL1基因序列设计引物,应用PCR技术扩增SPARCL1基因编码区（CDS）。将扩增产物连接到pMD18-T载体进行测序,获得小尾寒羊SPARCL1基因完整CDS区序列信息,应用生物信息学软件分析序列及蛋白结构。以小尾寒羊心脏、肝脏、肌肉、胃、十二指肠、小肠组织mRNA为模板,通过实时定量荧光PCR技术检测SPARCL1基因在小尾寒羊各组织间的表达差异。结果显示,试验成功获得小尾寒羊SPARCL1基因CDS 1 962 bp,编码653个氨基酸;分子质量为74.39 ku,理论等电点为4.64,为亲水性蛋白质;SPARCL1基因具有信息肽切割位点,为分泌蛋白;小尾寒羊SPARCL1基因序列与NCBI中绵羊序列同源性为99.80%,SPARCL1基因CDS区出现4处突变位点,但未引起氨基酸改变;SPARCL1蛋白存在77个蛋白磷酸化位点和3个糖基化位点;SPARCL1蛋白表达预测主要定位在细胞质;SPARCL1蛋白二级结构中α-螺旋、β-折叠、β-转角和无规则卷曲分别占31.9%、6.4%、28.7%和33.0%,三级结构预测结果与其一致。SPARCL1基因在小尾寒羊皮下脂肪组织中相对表达量明显高于其他组织。本研究成功克隆获得小尾寒羊SPARCL1基因CDS区完整序列,并对其序列、蛋白理化特性、结构及各组织间表达差异进行了详细分析,为研究小尾寒羊SPARCL1基因功能,探究其在小尾寒羊脂肪代谢过程中可能发挥的作用提供参考依据。 相似文献
15.
Ye L Zi C Pan ZY Zhu J Du ZD Zhu GQ Huang XG Bao WB Wu SL 《Comparative immunology, microbiology and infectious diseases》2012,35(1):23-30
Porcine post-weaning diarrhea and edema disease are principally caused by Escherichia coli strains that produce F18 adhesin. FUT1 genotyping and receptor binding studies divided piglets into E. coli F18-resistant and -sensitive groups, and the roles of SLA-1 and SLA-3 were investigated. SLA-1 and SLA-3 expression was detected in 11 pig tissues, with higher levels of SLA-1 in lung, immune tissues and gastrointestinal tract, and higher levels of SLA-3 also in lung and lymphoid tissues. Both genes were expressed higher in F18-resistant piglets, and their expression was positively correlated in different tissues; a negative correlation was observed in some tissues of F18-sensitive group, particularly in lung and lymphatic samples. Gene ontology and pathway analyses showed that SLA-1 and SLA-3 were involved in 37 biological processes, including nine pathways related to immune functions. These observations help to elucidate the relationship between SLA class I genes and E. coli F18-related porcine gastrointestinal tract diseases. 相似文献
16.
本研究以绵羊睾丸组织为材料,利用RT-PCR技术获得绵羊硫氧还蛋白类蛋白2(Thioredoxin like protein 2,Txl-2)基因的CDS区序列,并对其进行生物信息学分析。结果表明,克隆得到的绵羊Txl-2基因CDS区为795 bp,编码264个氨基酸。生物信息学分析结果表明,Txl-2编码的蛋白无跨膜区、信号肽和N-糖基化位点,存在多个磷酸化位点;预测出了Txl-2蛋白的二级结构和三级结构;Clustel W方法对比绵羊Txl-2与绵羊、山羊预测序列同源性最高。 相似文献
17.
本研究旨在克隆鸡淋巴细胞趋化因子（lymphotactin,XCL1）基因CDS区并探索其生物学特性。试验以固始鸡胸腺组织总RNA为模板,采用RT-PCR技术对该基因的CDS区进行扩增和克隆,并通过生物信息学软件进行相关生物信息学分析。结果表明,固始鸡XCL1基因CDS区长294 bp,编码97个氨基酸。相似性分析结果发现,鸡XCL1基因CDS区序列与牛、山羊、绵羊、人、小鼠、猪和大鼠的相似性分别为53.7%、53.0%、52.9%、51.8%、51.1%、50.9%和50.4%。进化树结果表明,鸡作为非哺乳动物,与哺乳动物亲缘关系较远。XCL1蛋白分子式为C491H822N150O132S6,理论等电点为10.95,属于碱性蛋白;分子质量为11.13 ku,脂肪系数为102.37,不稳定系数为51.44,属于不稳定蛋白,半衰期为30 h;具有跨膜结构（第5-27位氨基酸）和信号肽区域（第1-18位氨基酸）,属于亲水性分泌型蛋白。XCL1蛋白存在8个潜在的磷酸化修饰位点和3个潜在的糖基化修饰位点。XCL1蛋白的二级结构由α-螺旋（35.05%）、β-转角（5.15%）、延伸链（26.80%）和无规则卷曲（32.99%）组成,三级结构预测结果与二级结构一致。亚细胞定位分析表明XCL1蛋白主要在细胞外发挥作用;该蛋白有1个位于第31—88氨基酸残基处的SCY保守性结构域;该蛋白能与OXT、PTAFR、GPR132和XCR1等分子形成互作网络。本试验结果为进一步研究鸡XCL1基因功能提供理论参考。 相似文献
18.
Coddens A Verdonck F Mulinge M Goyvaerts E Miry C Goddeeris B Duchateau L Cox E 《Veterinary microbiology》2008,126(1-3):210-215
F18(+)Escherichia coli infections causing post-weaning diarrhoea and/or oedema disease are a major cause of economic losses in pig industry. To date, no preventive strategy can protect pigs from F18(+)E. coli infections. One of the most attractive approaches to eliminate F18(+)E. coli infections is the selection for pigs that are resistant to F18(+)E. coli infections. However, this strategy was not believed to be favourable because of reports of genetic association with the stress-susceptibility gene in the Swiss Landrace. To investigate this potential association more thoroughly, 131 randomly selected Belgian hybrid pigs were genotyped for both the F18(+)E. coli resistance alleles (FUT1(A)) and the stress-susceptibility alleles (RYR1(T)) and their association was investigated by determining the linkage disequilibrium. This linkage disequilibrium (LD=-0.0149) is close to zero and does not differ significantly from 0 (likelihood ratio test chi(1)(2)=1.123, P=0.29), demonstrating no association between the FUT1(A) and RYR1(T) alleles. Furthermore, only a small fraction (4.6%) of the Belgian pigs was found to be resistant to F18(+)E. coli infections. Our results suggest that selection for F18(+)E. coli resistant pigs might be an attractive approach to prevent pigs from F18(+)E. coli infections, unlike to what has previously been postulated. 相似文献
19.
本研究旨在克隆驴Zfx基因CDS序列,并对其进行生物信息学分析。根据GenBank中公布的牛Zfx基因mRNA序列（登录号：D84097.1）设计特异性引物,利用RT-PCR技术获取驴Zfx基因CDS序列,并对驴Zfx基因CDS区核苷酸序列和蛋白质结构进行生物信息学分析。结果表明,驴Zfx基因CDS区序列长度为2 403 bp,编码800个氨基酸;驴与马、牛、家犬、白犀牛、马鹿、猫、人、绵羊、黑猩猩的Zfx基因CDS区核苷酸序列同源性分别为99.6%、95.0%、95.3%、97.9%、93.5%、94.9%、95.0%、95.7%和94.9%。对驴及其他9种哺乳动物Zfx基因CDS区的核苷酸序列构建系统进化树,结果显示,驴与马亲缘关系很近,与白犀牛的亲缘关系次之;同时又与聚在一类的家犬、猫亲缘关系较近,而与绵羊、牛的亲缘关系稍远。Zfx蛋白理论等电点（pI）为5.19,不稳定系数为42.94,消光系数为35 810,属于不稳定的亲水性蛋白,含有60个磷酸化位点,无信号肽及跨膜结构,属于非分泌蛋白。Zfx蛋白α-螺旋、延伸链和无规则卷曲分别为23.12%、20.25%和56.63%,三级结构主要为α-螺旋和无规则卷曲。本试验成功克隆获得驴Zfx基因CDS序列,为后期深入研究该基因及其编码蛋白的结构功能提供依据。 相似文献