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1.
《渔业科学进展》2017,(1)
为了开发适宜牙鲆(Paralichthys olivaceus)苗种放流的标志技术,利用编码金属标签(CWT)对不同规格牙鲆苗种进行了标记实验,并从标记死亡率、脱标率、适宜标记鱼规格等方面评价了CWT的标记效果。结果显示,CWT标记3种规格的牙鲆苗种后,脱标均发生在标记后的4 d内,其后未再发生脱标现象。小规格苗种[全长为(5.92±0.41)cm]死亡率较高(13%),中规格苗种[全长(8.92±0.36)cm]死亡率为2%,大规格苗种[全长为(12.06±0.62)cm]死亡率为1%。小规格、中规格和大规格苗种脱标率分别为3.3%、2.4%和0.7%。建立了标记死亡率(M)与苗种全长(TL)、体厚度(BT)的关系模型:M=0.7254 TL~2–15.3220TL+79.4260(R~2=0.9601);M=1.3627 BT~2–15.5610 BT+44.4330(R~2=0.9645),为适宜苗种规格选择与标记效果评价提供了依据。今后利用CWT标签进行牙鲆苗种标志放流时,建议选择全长6 cm以上的苗种进行背部肌肉标记,标记对苗种游泳行为和生长无影响,表明CWT是一种适宜在牙鲆大规模标志放流中应用的理想标志方式。 相似文献
2.
摘要以牙鲆为实验对象,研究了4种不同规格的T型标志牌（T-A、T-B、T-C和T-D）对全长5、7、9和11cm苗种的标记效果,确立了适宜标志的牙鲆苗种规格,筛选了适宜于不同规格牙鲆苗种标记用的T型标志牌。全长5cm的苗种不宜使用这4种规格的标志牌标记;T-A和T-B标志牌适宜于全长7～11cm的牙鲆苗种,标记后脱牌率为2％～8％,存活率为90％～100％;T-C标志牌适宜于标记全长11cm的苗种,成活率为94％,脱牌率为5％,对全长7～9cm苗种的标志效果不佳,成活率为85％～87％,脱牌率为9％～11％;T-D标志牌不适宜于全长11cm以下牙鲆苗种的标记,脱牌率为11％～26％,成活率为51％～89％。2011～2012年,利用T-B标志牌在北戴河开展了牙鲆大规模标志放流试验,取得了良好的标记效果。 相似文献
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《福建水产》2021,(2)
以大泷六线鱼为研究对象,研究了3种不同规格的T型标志牌(T1、T2、T3)对平均全长5、8和11 cm鱼种的标志效果,记录了标志后鱼种的存活率、脱标率、行为特征及生长情况,筛选了适宜不同规格大泷六线鱼标志放流的T型标志牌。结果显示:平均全长5 cm及以下的鱼种不适宜标志放流;平均全长8 cm的鱼种适宜用T1标志牌,标志后脱标率为4.83%,存活率为97.17%;平均全长11 cm的鱼种适宜用T1和T2标志牌,标志后脱标率分别为2.00%和5.67%,存活率分别为100%和95.83%。2017年6月,在烟台近海开展了T1标志牌标志大泷六线鱼的放流试验,取得了良好的标志效果。 相似文献
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利用金属编码标签(coded wire tag, CWT)对不同规格许氏平鲉(Sebastes schlegelii)放流苗种的不同部位(鳃部鄂弓提肌和背部肌肉)进行了标记实验,从标记苗种成活率、标签保持率、标记后苗种抗氧化酶活性及生长应激相关基因表达等方面评价了CWT的标记效果。结果显示,CWT标记30 d后,不同规格苗种成活率均为100%,小规格苗种鄂弓提肌标记组(SLM组)的实验鱼标签保持率为87%,大规格苗种鄂弓提肌标记组(LLM组)的实验鱼标签保持率为97%,苗种背部肌肉标记的标签保持率均为100%。CWT标记实验鱼特定生长率低于对照组。标记鱼暂养后,SLM组和大规格背部肌肉标记组(LDM)实验鱼肝脏过氧化氢酶(CAT)活性显著高于对照组,所有组实验鱼肝脏超氧化物歧化酶(SOD)活性均显著高于对照组。除SDM组外,其他实验组肝脏中IGF-1 mRNA表达水平显著高于对照组,LDM组肝脏HSP70 mRNA表达水平最低。从存活率与标签保持率的角度考虑,CWT适宜于许氏平鲉苗种的标记放流,但CWT标记的苗种生长慢于对照组,且在免疫相关酶活性和生长及应激基因方面存在显著的表达差异,表明CWT标记操作对许氏平鲉苗种造成了一定的生理胁迫。因此,利用CWT标记的许氏平鲉苗种应有一个相对较长的室内暂养或野性驯化时间,有利于提高标记放流苗种成活率和放流后生长及回捕。 相似文献
5.
为了探明适宜大渡河安谷水电站流域增殖放流鱼类标志的方法,在安谷水电站鱼类增殖放流站使用T-tag锚标法(T-tag)、金属线码标法(CWT)和荧光胶体标记法(VIE)分别对四种放流鱼苗(胭脂鱼Myxocyprinus asiaticus、长薄鳅Leptobotia elongate、唇■Hemibarbus labeo和黄颡鱼Pelteobagrus fulvidraco)进行标记。每种鱼苗选择大、小两种规格进行试验。在标记后40 d分别统计了鱼苗的存活率、标记保持率和生长等数据。结果表明:(1)40 d短期标记条件下,CWT和VIE对四种鱼苗的存活率负面影响较小,T-tag对存活率负面影响较大。其中,CWT和VIE对体长50～110 mm小规格放流鱼苗的存活无显著影响,而T-tag导致小规格放流鱼苗存活率显著下降;(2)四种放流鱼苗CWT和VIE标记保持率均显著高于T-tag,其中,除50～80 mm的小规格长薄鳅VIE组标记保持率略低于CWT外,其它各实验组均表现出VIE组大于CWT组;(3)CWT和T-tag标记保持率与标记鱼类生活习性相关性较大,胭脂鱼、长薄鳅标记保持率相对高于唇■、黄颡鱼;(4)三种标记对鱼苗都具有一定的损伤,但与对照组相比标记组存活的鱼苗生长状况均不受影响,其中,四种鱼苗相比唇■的生长速度最快。(5)考虑到T-tag标记具备操作简单、标记快、易辨认等特点,建议使用T-tag标记时鱼苗的全长应大于170 mm。 相似文献
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于2003年5月～2004年4月使用编码微型金属标(Coded wire tag,CWT)系统标志鳜[Siniperca chuatsi (Basilewsky)]稚鱼[全长(44.3±3.4)mm,(X)±SD],观测在不同位置标记的鳜的生长情况,检验了CWT标记不同部位对鳜稚鱼存活、生长的影响以及标记保存率.结果表明,微型金属标注入鳜稚鱼的背部、腹部及尾柄肌肉的标志方法是可行的.28 d的短期和305 d的长期实验后,CWT标志以上3个位置对鳜稚鱼的存活、生长无显著性影响,3个位置的标志保持率无显著差异.其中背部肌肉标志操作最为简便.CWT标志鳜稚鱼背部肌肉后,第一阶段1～28 d实验期间内,标志鳜的存活率为91.7%,标志保存率为97.9%;第二阶段29～333 d实验期间内标志鳜的存活率和标志保存率都为100%.这一结果表明,CWT标志鳜背部肌肉方法可用于鳜的标志放流回捕研究. 相似文献
9.
可见植入荧光标记和编码金属标对达乌尔鳇标志效果的初步研究 总被引：2，自引：0，他引：2
2011年9月底至2012年5月,采用可见植入荧光标记(VIE)和编码金属标(CWT)标志全长为(28.06±1.79)cm的达乌尔鳇(Huso dauricus),检验了VIE和CWT的标志保持率及其对达乌尔鳇生长和存活的影响。结果显示,经过210 d实验,VIE标志达乌尔鳇吻部腹面,CWT标志达乌尔鳇背部前(第1～2块背骨板间下方肌肉)、中(第6～7块后背骨板间下方肌肉)、后(第11～12块背骨板间下方肌肉)的标志保持率分别为:95.83%、92.00%、92.59%、92.59%。实验中VIE出现破碎、脱落或迁移致使标志可识别性下降;CWT背部中这一标志部位操作最为简便。标志30 d,VIE标志组的保持率较CWT背部不同部位标志组及对照组低,且差异显著(P<0.05),此后31～210 d并无显著性差异。VIE和CWT背部不同部位标志对达乌尔鳇的生长无显著性差异。结果表明,VIE和CWT均可用于标志达乌尔鳇。进行短期大规模达乌尔鳇的标志放流可使用成本相对较低的VIE标志。 相似文献
10.
金属线码标记技术在渔业生物增殖放流中的应用 总被引：1，自引：0，他引：1
近年来,由于海洋渔业资源的过度利用及环境污染等问题不断出现,致使中国近海海洋生物资源不断衰退,开展增殖放流成为修复渔业资源较有效的方式之一,其中放流种类的生物标志技术是评价放流效果的重要手段。金属线码标记(CWT)作为渔业生物体内标志的一种重要方法,已在放流种类种群密度、存活率、资源补充量估算及洄游分布等研究中广泛应用。本文系统整理了国内外开展CWT研究的相关资料,介绍其原理和发展历史,简述在渔业生物增殖放流中的应用,并分析其优势和缺点。综合看来,CWT具有标记速度快、对鱼体损伤小、标记保持率高、批量标记价格低廉等优点,同时也有回捕率较低的缺点。随着渔业生物标志技术的不断发展,CWT在渔业资源增殖放流领域的应用将更广泛,并成为标志放流研究的发展方向之一。 相似文献
11.
Mohamed Soula Ana Navarro Silvia Hildebrandt María J. Zamorano Javier Roo Carmen M. Hernández-Cruz Juan M. Afonso 《Aquaculture International》2012,20(3):571-583
The effect of two physical tagging systems, Visible Implant Elastomer (VIE) and Passive Integrated Transponder (PIT), was evaluated in red porgy (Pagrus pagrus) fingerlings, at different sizes. For VIE tagging, the weight classes were <1 and 1?C5?g and no tag effect was detected on fish growth. There was a significant difference in mortality between tagged and untagged <1?g fish, but not for the 1?C5?g fish. The tag loss rate was null for all sizes, however, tags showed fragmentation. This partial tag loss was evaluated and quantified. Seven VIE colours were compared and arranged from best to worst visibility: green, red, pink, orange, yellow, white and blue. For PIT tagging, 2?C6?g, 5?C10?g and 10?C20?g weight classes were analysed. There was no significant tag effect on fish growth, for any size, nor on mortality from 10?g. Tag loss rate ranged from 2.9 to 5.9%. These results demonstrate that, in red porgy, VIE is a successful tagging system from 2?g onwards whereas PIT system is successful from 10?g onwards. The combination of both systems allows traceability of fish from a very small size on, which is necessary for the implementation of genetic breeding programmes. 相似文献
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Toshinori Isojima Naoshi Makino Masato Takakusagi Masatomo Tagawa 《Fisheries Science》2013,79(5):787-797
Abnormal flounder coloration frequently occurs in flounder hatcheries and diminishes the commercial value of the fish. To understand hypermelanosis, the progression of staining-type hypermelanosis in normally metamorphosed juveniles and ocular-side pigmentation in pseudoalbino juveniles were examined in the Japanese flounder Paralichthys olivaceus. Sixty-five days post hatching, juveniles (total length, 6 cm) were individually identified by color-marker implantation, and the darkened area of the body surface was examined for 10 weeks by image analysis of digital photographs of the fish taken from above or below the transparent tank. Staining was observed to mainly begin at the upper and lower bases of the tail fin, expanded anteriorly along the peripheral part of the trunk, and ceased after 2 months. The individuals in which staining occurred earlier expressed severe staining and small body size by the end of the experiment. Further, pigmentation of the ocular side in pseudoalbino juveniles ceased after 2 months, but the order of pigmentation was different from that on the blind side. In this case, darkening began from the posterior, but expanded from the center to the periphery of the trunk. Even at the end of the experiment, ctenoid scales were exclusively found within the darkened area, together with cycloid scales. 相似文献
13.
《Journal Of Applied Aquaculture》2013,25(3-4):133-142
Abstract Attempts to rear summer flounder, Paralichthys dentatus, in net pens have suffered from high mortality during the month after fish were transferred from a nursery facility to pens. We investigated whether exposing summer flounder to increased (and constant) current velocity in the nursery stage would condition them for better performance in cages. Three experiments were conducted with different water velocities in tanks, using fish of 124±4 g (exposed to 0, 15, or 30 cm/sec for 60 d), 257±12 g (exposed to 0, 20, or 40 cm/sec for 60 d) and 387±13 g (exposed to 0, 15, or 30 cm/sec for 30 d), in a raceway system with adjustable paddlewheels. For all of the size groups of fish, survival was significantly reduced at the highest current velocity. For both 124-g and 257-g fish, growth in the medium-velocity treatment was significantly better than that in the control (0 cm/sec) treatment, which in turn was better than that in the high-velocity treatment. For 387-g fish, growth at medium velocity was significantly greater than that at high velocity; control fish were lost due to a system malfunction. Food consumption data from the 257-g fish showed that the fish in medium velocity grew most because they consumed significantly more food during the experiment than did fish in the control, which in turn consumed significantly more than fish in high velocity. Similarly, 387-g fish in medium velocity consumed the most food, but the difference was not significant in this case. At the end of the laboratory experiment with 124-g fish, fish from the control and medium-velocity treatments were moved to cages in Narragansett Bay, Rhode Island, where currents of about 1 knot (approx. 55 cm/second) are routine. After three weeks in the cages, no significant differences in survival were observed (control = 83±12%; medium velocity = 81±2%). Subsequent damage to some of the cages and escapement of the fish precluded further statistical analysis of survival, as well as any growth measurements. At the end of the laboratory experiment with 387-g fish, fish from medium- and high-velocity treatments were moved to the cages; however, survival in this case was very low (<10% in all cages), perhaps due to heavy waves from an offshore hurricane. We conclude that current velocities of 15-20 cm/second in the nursery improve growth of juvenile summer flounder, that current velocities of 30-40 cm/second are excessive, but that increased current velocity in the nursery does not improve fish survival upon transfer to cages. 相似文献
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5种鱼类标志对草鱼临界游泳速度的影响 总被引：1，自引：0，他引：1
采用被动整合雷达标志法(PIT)、切鳍标记、荧光标记、超声波标志和T型标志这5种标志方法对草鱼(Ctenopharyngodon idellus)进行标记后,测定其临界游泳速度,研究5种不同标记对草鱼游泳能力的影响。将试验草鱼按不同体长分为3组:15~18 cm,18~21 cm,21~25 cm,每个体长组均设置对照组,并分别进行以上5种鱼类标志,测量标志后草鱼的绝对临界游泳速度和相对临界游泳速度。采用SPSS17.0统计软件进行数据的分析比较。研究结果表明,随着体长增加,草鱼的绝对临界游泳速度增大,相对临界游泳速度减小;超声波标志对3个体长组草鱼的临界游泳速度均有极显著影响(P0.01),标志后草鱼绝对临界游泳速度分别下降18.72%、16.40%、23.15%,相对临界游泳速度分别下降18.95%、17.78%、21.86%;T型标志对15~18 cm体长组草鱼的临界游泳速度有极显著性影响(P0.01),标志后草鱼绝对临界游泳速度下降8.35%,相对临界游泳速度下降9.30%,对体长大于18 cm的草鱼无显著性影响;PIT标志、切鳍标记和荧光标记对这3个体长组草鱼的临界游泳速度均无显著性影响(P0.05)。 相似文献
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A. Sánchez-Lamadrid 《Fisheries Management and Ecology》2001,8(3):271-278
This study tested different tags for each size of farm-reared gilthead sea bream, Sparus aurata L. juveniles, used in enhancement programmes. The following tags were tested: dye mark, fingerling tag, opercular tag, anchor tag A (filament diameter of 1.20 mm) and anchor tag B (0.85 mm). Tag/mark retention, wounds produced by the tag, ease of application, ease of detection and cost were examined. Epidermal dye marks had poor retention and detection rates. Of the tags tested, retention was greater for the anchor tag A (80–100%) after 7 months; anchor tag B showed a retention rate of only 20% after 3 months. The fingerling tag had a retention rate of 27.5% after 12 months. The opercular tag had a retention rate of 44% after 20 days but fractured the opercular bone in many cases. Tag incisions and wounds persisted in some fish for the duration of the experiment. 相似文献
16.
Effects of tagging on migration behaviour,survival and growth of hatchery‐reared Atlantic salmon smolts 
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下载免费PDF全文 R. Huusko A. Huusko A. Mäki‐Petäys P. Orell J. Erkinaro 《Fisheries Management and Ecology》2016,23(5):367-375
The effects of four different tagging methods (PIT, anchor T‐tag, Carlin tag and dummy radio transmitter) on survival, behaviour and growth of Atlantic salmon smolts during their downstream migration were examined in semi‐natural circular channels during a natural migration period in spring. Survival of smolts was high and tagging wounds healed well in all tagging groups. Tag loss rates were generally low, being the highest (2.5%) in the dummy radio transmitter group. Total length and body mass of the tagged and untagged smolts did not differ at the end of the experiment. Migration activity of smolts generally showed similar patterns among the treatments. However, Carlin‐tagged smolts started their migration slightly later than the PIT‐tagged fish, and smolts tagged with Carlin tag or dummy radio transmitter showed less overall migration activity than fish with PIT tag. 相似文献
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In tropical fisheries where ageing fish is often difficult, tagging programmes offer an important means to estimate growth parameters and mortality rates. The majority of tags are large and attached externally via a puncture in the muscle. These characteristics may cause significant biological effects which bias parameter estimates. Visible implant (VI) tags offer a more benign means to individually mark fish. The utility of VI tags for marking five species of tropical river fish was examined. The results indicate that the tag and tagging operation had no influence on either growth or mortality, although tag retention was poor compared with other studies. For three of the five species, the only suitable tagging location was highly inconspicuous and therefore inappropriate for tagging programmes that rely upon fishermen to return tagged fish. Alternative tagging locations may improve retention rates and the visibility of the tag. 相似文献