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1.
将鸡传染性法氏囊病免疫复合物疫苗和BX株活疫苗分别免疫1日龄中等母源抗体水平京红雏鸡,同时免疫1日龄SPF雏鸡作对照,免疫后第9天观察法氏囊病变,免疫后第28天采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后第9天,免疫复合物疫苗组SPF雏鸡和京红雏鸡法氏囊均正常,BX株活疫苗组SPF雏鸡法氏囊出现了萎缩病变;免疫后第28天,对照SPF雏鸡和京红雏鸡的免疫复合物疫苗组、BX活疫苗组IBDV中和抗体效价分别为9.2log2、8.5log2和 7.2log2、4.4log2,攻毒保护率均为100%。试验结果表明免疫复合物疫苗免疫1日龄中等水平母源抗体雏鸡效果很好,能产生较高的中和抗体,攻毒保护率能达到100%。  相似文献   

2.
为比较鸡传染性法氏囊病(IBD)免疫复合物疫苗与活疫苗免疫鸡法氏囊及外周血淋巴细胞(PBMC)中的病毒载量及免疫效果,本研究采用IBD免疫复合物疫苗和鸡传染性法氏囊病毒(IBDV)BX株活疫苗免疫1日龄SPF鸡,于免疫后7d、14d、21d、28d、35d、42d采用SYBRGreenI荧光定量PCR、ELISA方法及中和试验检测免疫鸡法氏囊和PBMC中IBDV载量和免疫鸡血清中IBDV抗体滴度,并攻毒,计算两种疫苗的保护率。结果显示,活疫苗免疫7d时IBDV在法氏囊和PBMC中的载量均比其它时间点的高,在14d时到达到最高,之后逐渐下降;两种疫苗刺激产生的IBDV抗体滴度随免疫时间的延长呈升高趋势;在免疫后7d攻毒两种疫苗的保护率均为0,其它时间的攻毒保护率均为100%。本研究结果表明,IBD免疫复合物疫苗与活疫苗免疫后病毒在鸡体内开始大量复制的时间并不相同,但免疫效果基本相同。本实验为IBD免疫复合物疫苗的研发提供实验依据。  相似文献   

3.
用鸡传染性法氏囊病免疫复合物疫苗和活疫苗分别免疫1日龄SPF雏鸡,免疫后9d观察法氏囊病变,免疫后14、21、28d对各组鸡采血测定IBDV中和抗体,21d及28d时用强毒攻击,计算各组疫苗保护率。结果表明,免疫后9d免疫复合物疫苗组鸡法囊正常,活疫苗组鸡法氏囊全部明显萎缩;免疫后14、21、28d,免疫复合物疫苗组鸡IBDV中和抗体分别为2.9±0.99、8.1±0.88和10.6±1.78,活疫苗组IBDV中和抗体分别为3.3±1.14、8.3±1.16和9.2±1.23,免疫后21d及28d疫苗免疫组均100%保护。试验证明免疫复合物疫苗比活疫苗更为安全,免疫复合物疫苗免疫SPF雏鸡产生比活苗更高的IBDV中和抗体,保护率达100%。  相似文献   

4.
为评估鸡新城疫(ND)-传染性支气管炎(IB)-传染性法氏囊病(IBD)三联灭活疫苗对不同日龄和不同水平母源抗体雏鸡的免疫效力和持续期,本试验用该疫苗免疫7、14、21日龄SPF雏鸡和有母源抗体的普通雏鸡,免疫后采血测定ND血凝抑制抗体(HI Ab)、IB血凝抑制抗体(HI Ab)及IBD中和抗体(NA),并用传染性法氏囊病病毒(IBDV)强毒攻击。结果显示,7日龄SPF雏鸡免疫后21 d ND HI抗体、IB HI抗体及IBD中和抗体效价分别为7.9log2、6.9log2和14.1log2,SPF鸡日龄越大,抗体水平越高;28日龄SPF鸡免疫后3个月,0.3 mL免疫剂量组试验鸡ND HI、IB HI及IBD中和抗体效价分别达6.5log2、6.1log2和13.6log2,IBDV攻毒保护率均为100%(10/10);不同日龄普通雏鸡免疫效果与SPF鸡试验一致,抗体水平随鸡日龄增大而升高,IBD攻毒保护率也都达到100%(10/10)。试验结果证实,鸡新城疫-传染性支气管炎-传染性法氏囊病三联灭活疫苗可使7、14及21日龄SPF雏鸡和普通雏鸡产生良好的免疫力,对雏鸡的免疫期至少为3个月。  相似文献   

5.
为评估鸡新城疫（ND）-传染性支气管炎（IB）-传染性法氏囊病（IBD）三联灭活疫苗对不同日龄和不同水平母源抗体雏鸡的免疫效力和持续期,本试验用该疫苗免疫7、14、21日龄SPF雏鸡和有母源抗体的普通雏鸡,免疫后采血测定ND血凝抑制抗体（HI Ab）、IB血凝抑制抗体（HI Ab）及IBD中和抗体（NA）,并用传染性法氏囊病病毒（IBDV）强毒攻击。结果显示,7日龄SPF雏鸡免疫后21 d ND HI抗体、IB HI抗体及IBD中和抗体效价分别为7.9log2、6.9log2和14.1log2,SPF鸡日龄越大,抗体水平越高;28日龄SPF鸡免疫后3个月,0.3 mL免疫剂量组试验鸡ND HI、IB HI及IBD中和抗体效价分别达6.5log2、6.1log2和13.6log2,IBDV攻毒保护率均为100%（10/10）;不同日龄普通雏鸡免疫效果与SPF鸡试验一致,抗体水平随鸡日龄增大而升高,IBD攻毒保护率也都达到100%（10/10）。试验结果证实,鸡新城疫-传染性支气管炎-传染性法氏囊病三联灭活疫苗可使7、14及21日龄SPF雏鸡和普通雏鸡产生良好的免疫力,对雏鸡的免疫期至少为3个月。  相似文献   

6.
为了评估鸡新城疫(ND)-传染性法氏囊病(IBD)-禽流感(AI)(H9亚型)三联灭活疫苗对雏鸡的免疫效力,采用该疫苗分别免疫1、7、10、14、21日龄SPF雏鸡和带有母源抗体的普通雏鸡,免疫后21d~28d采血测定ND血凝抑制抗体(HIAb)、IBD中和抗体(NA)及AI(H9)HIAb抗体,并用传染性法氏囊病病毒(IBDV)强毒攻击。结果显示,免疫后各不同日龄免疫组SPF雏鸡的ND HIAb、IBD NA及AI(H9)HIAb滴度分别为7.9log2~8.3log2、13.9log2~15.8log2及8.0log2~8.9log2,IBDV攻毒均100%保护;而普通雏鸡免疫后的ND HIAb、IBD NA及AI(H9)HIAb滴度,1日龄免疫组分别为5.9log2、12.1log2和5.6log2,7、10、14、21日龄免疫组分别为6.1log2~7.0log2、12.6log2~14.5log2和6.0log2~7.5log2;IBDV攻毒,1日龄免疫组普通雏鸡保护率为60%,7、14及21日龄免疫组保护率均为100%;结果证实,SPF雏鸡所产生的免疫效力要高于带有母源抗体的普通雏鸡,1日龄普通雏鸡免疫所产生的免疫效力明显低于其他日龄。根据试验结果,推荐该疫苗对雏鸡的使用日龄为7日龄~14日龄。  相似文献   

7.
采用在DF1细胞上增殖的高滴度鸡传染性法氏囊病(IBD)病毒制备油乳剂灭活疫苗免疫黄羽肉鸡种鸡和雏鸡,以探讨IBD灭活疫苗(HQ株)对黄羽肉鸡的免疫效果。种鸡免疫试验结果显示:种母鸡体内产生高水平鸡传染性法氏囊病病毒(IBDV)血清中和抗体,免疫30 d后血清中和抗体均值可达18.5 log2,免疫150 d后血清中和抗体均值仍维持在16.0 log2;高水平的种鸡抗体能使子代获得高水平的母源抗体,使子代在出生后3周内能有效抵御IBDV强毒攻击。雏鸡免疫试验结果显示,无论是高母源抗体雏鸡或低母源抗体雏鸡均可在免后3周产生12.0 log2的中和抗体并持续上升,可在2个月内抵抗IBDV强毒的攻击。提示IBD灭活疫苗(HQ株)可用于出壳雏鸡的主动免疫。  相似文献   

8.
用鸡法氏囊病免疫复合疫苗(IBDV-Icx)和常规IBD活疫苗分别免疫1日龄商品鸡和SPF鸡,进行疫苗的安全性及免疫效力比较.免疫后8d检测,IBDV-Icx免疫组鸡的法氏囊未见明显萎缩,而传统弱毒疫苗免疫组鸡的法氏囊萎缩明显;免疫后28d各组用IBDV标准强毒株进行攻击,IBDV-Icx免疫组鸡的攻毒保护率均为10/10,常规IBD活疫苗对照组分别为8/10及9/10.免疫后3个月,IBDV-Icx免疫组血清抗体可达AGP1∶32,攻击强毒仍为10/10保护,而常规IBD活疫苗免疫后2个月抗体AGP为0,攻毒保护率为1/10,免疫后3个月时攻毒10/10发病.  相似文献   

9.
本研究用传染性法氏囊病活疫苗(Gt株)免疫3周龄SPF雏鸡,免疫两周后,分别用IBDV超强毒株HLJ-4株与Gx株进行攻毒,攻毒剂量为10ELD50/0.1ml,攻毒结果表明:传染性法氏囊活疫苗(Gt株)能抵抗HLJ-4和Gx的攻击,SPF鸡保护率达100%。  相似文献   

10.
《畜牧与兽医》2015,(5):7-11
为探讨鸡新城疫病毒(NDV)、传染性法氏囊病毒(IBDV)二联灭活疫苗在蛋雏鸡养殖中的实际应用效果进行本试验。用鸡新城疫、传染性法氏囊二联灭活疫苗及传染性法氏囊活疫苗以不同免疫程序免疫7~14日龄蛋雏鸡,定期监测NDV、IBDV抗体水平,并在雏鸡60日龄时进行攻毒保护试验。结果表明,各二联苗免疫组NDV抗体水平在免疫后14 d即达到NDV血清学最低有效保护价标准,并维持至雏鸡60日龄。免疫后28 d,灭活苗一次性免疫各组IBDV中和抗体水平显著高于活苗两次免疫组,琼扩抗体水平与活苗两次免疫组无明显差异。雏鸡60日龄时攻毒,各免疫组均100%保护,对照组无保护。以上结果说明该二联灭活疫苗可替代IBDV活毒疫苗用于7~14日龄商品雏鸡免疫,免疫期至少为60d。  相似文献   

11.
Five kinds of infectious bursal disease (IBD) immune complex (IC) vaccines were prepared with infectious bursal disease virus (IBDV) BX strain mixed with IBDV hyperimmune serum according to a certain proportion (containing 32, 8, 4, 0.5 and 0.125 units IBDV neutralizing antibody, respectively).One-day old low maternal antibody chickens were vaccinated with IBD IC vaccines 1 to 5 and BX strain live vaccine, respectively, pathological changes of the bursa of fabricius in chickens were observed at the 9th day after immunization.On the day of 28 after immunization, blood samples were taken and the IBDV neutralizing antibody were tested, meanwhile all experimental chickens were challenged with high virulent IBDV, the protective rates of vaccines were calculated.The results showed that at the 9th day after immunization, the bursa of fabricius were normal in IC vaccine 1, 2 and 3 groups, however 2/10, 4/10 and 5/10 of pathological changes of the bursa of fabricius in IC vaccine 4, 5 groups and BX strain vaccine group, respectively.At the 28th day after immunization, the IBDV neutralizing antibodies in IC vaccine 1, 2, 3, 4, 5 groups and BX strain vaccine group were 8.34log2, 9.60log2, 9.21log2, 7.88log2, 9.50log2 and 9.12log2, while 90%, 100%, 100%, 80%, 90% and 80% protection rates were provided, respectively.The results showed that IBD IC vaccines 2 and 3 (containing 8, 4 units IBDV neutralizing antibody, respectively) had the best immunity effect on one-day old low maternal antibody chickens, protection rates were both 100%.  相似文献   

12.
One-day-old moderate maternal antibody Jinghong chickens and SPF chickens were vaccinated with infectious bursal disease (IBD) immune complex (IC) vaccine and BX strain live vaccine,respectively,pathological changes of bursa of fabricius in chickens were observed at the 9th day after immunization.On the 28th day after immunization,blood samples were taken and the IBDV neutralizing antibody were tested,meanwhile experimental chickens were challenged with high virulent IBDV,the protective rates of experimental groups were calculated.The results showed that at the 9th day after immunization,the bursa of fabricius in SPF and Jinghong chickens were normal in IC vaccine group,however atrophic changes of bursa of fabricius in BX strain live vaccine group were shown.At the 28th day after immunization,the IBDV neutralizing antibody of IC vaccine group and BX strain live vaccine group in SPF and Jinghong chickens were 9.2log2,8.5log2,7.2log2 and 4.4log2,respectively,while 100% protection rates were provided.The results provided that IC vaccine had better immunity effect on one-day-old moderate maternal antibody chicken,high levels of neutralizing antibodies against IBDV were produced and protection rates were 100%.  相似文献   

13.
为了选出法氏囊疫苗免疫效果评估的合适方法,本研究使用酶联免疫吸附试验(ELISA)、琼脂糖扩散试验(AGP)和中和抗体检测3种方法对6种法氏囊灭活疫苗免疫后28 d的抗体水平进行评估,同时,对采血鸡进行攻毒,攻毒后4 d剖检所有鸡,观察法氏囊病变,评估3种抗体水平与攻毒保护之间的相关性;结果显示,ELISA和AGP方法检测免后血清结果相对较高,中和试验方法检测免后抗体值较低;ELISA和AGP方法检测疫苗免后抗体水平与攻毒保护结果基本一致,使用中和试验方法检测免后抗体与攻毒保护相关性较差;建议在实际生产中使用ELISA和AGP方法进行法氏囊疫苗免疫效果的评估。  相似文献   

14.
The evolution of very virulent (vv) infectious bursal disease virus (IBDV) has led to significant economic losses in many poultry-producing areas. Despite vigorous vaccination strategies, IBDV has been difficult to control. The protective efficacy of IBDV vaccines is traditionally evaluated in specific pathogen-free (SPF) chickens. But under field conditions, residual maternal antibody (mAb) levels may interfere with vaccine efficacy. In this study, commercial broilers with various levels of maternally derived antibodies were vaccinated with IBDV vaccines of different virulence (vaccines 1-3, intermediate; vaccine 4, intermediate plus). At an average maternal virus-neutralizing antibody (mAb) level of log2 10.8 (range 7.6-11.6) at day of vaccination, only the intermediate plus vaccine induced IBDV antibodies after 18 days, while the other intermediate vaccines did not. At average mAb levels of log2 6.7 (range 5.6-8.6) at day of vaccination, all vaccines induced circulating antibodies, although the onset of antibody production differed significantly between strains (P < 0.05). While the intermediate plus vaccine induced enzyme-linked immunosorbent assay antibody levels already at 14 days postvaccination (PV), the intermediate vaccines induced significant antibody levels 28 (vaccines 1, 2) and 35 (vaccine 3) days PV. The time of IBDV antibody induction correlated with the onset of bursa lesions. The severity of lesions was comparable between vaccines 1, 3, and 4 (lesion score 4), while vaccine 2 induce only mild lesions of score 1 in 23% of the tested birds. Despite the induction of antibodies, none of the tested vaccines fully protected against challenge with vvIBDV. All challenged birds had either significantly higher bursal lesion scores or a higher IBDV antigen load in the bursa or sometimes both in comparison with nonchallenged birds (P < 0.05). Our study demonstrates that the evaluation of IBDV-vaccine efficacy is difficult in commercial broilers. For the first time, it was shown that the onset of bursa lesions and recovery of IBDV-vaccinated broilers is delayed in the presence of mAb in comparison with SPF chickens but not suppressed as previously assumed. At the time of challenge, vaccinated birds may still have significant bursa lesions and may lack target cells for IBDV-challenge virus. To be able to evaluate vaccine efficacy in commercial broilers, parameters such as intrabursal IBDV-antigen load should also be considered in conjunction with bursa lesion scores.  相似文献   

15.
Infectious bursal disease (IBD) causes severe economic damage to the poultry industry worldwide. To prevent IBD virus (IBDV) infection, live virus vaccines have been widely used in chickens having wide-ranging levels of maternally derived antibodies. But, the risks of infection with other pathogens because of lesions related to atrophy of the bursa of Fabricius in vaccinated chickens are a concern. To resolve the problems, a recombinant turkey herpesvirus (HVT) vaccine expressing IBDV-VP2 protein (rHVT-IBD) has been developed. However, the induction of neutralizing antibodies by rHVT-IBD against a virulent IBDV might be delayed compared with that by the live IBD vaccine, leading to the high risks of IBDV infection for young chickens. To find the best selection of IBDV vaccine for the onset of immunity, we examine the protective efficacy of a novel in ovo-attenuated live IBDV (IBD-CA) vaccine and the rHVT-IBD vaccine in young chickens challenged with a very virulent IBDV (vvIBDV) strain. We show that the protective efficacy of IBD-CA vaccine was higher than that of the rHVT-IBD vaccine in 14-day-old chickens challenged with the vvIBDV strain, leading to the risk of IBDV infection for young chickens when vaccinated with rHVT-IBD. Our results suggest that farmers should select the best vaccines to maximize vaccine efficacy in consideration of the vaccine characteristics, prevalence levels of IBDV in the areas, and initial MDA levels of the chickens since the attenuated live and recombinant vaccines play a role in the different vaccine efficacies.  相似文献   

16.
The appearance of very virulent strains of infectious bursal disease (IBD) virus at the end of the 1980s made it necessary to develop more effective immunization procedures. To facilitate this, the immunogenicity and the immunosuppressive effect of a mild (G-87), an intermediate (LIBD) and an intermediate-plus (IBDV 2512) IBDV strain were tested after the in ovo inoculation of 18-day-old SPF and broiler chicken embryos. It was established that no noteworthy difference existed between the immunized and the control embryos in hatching rate and hatching weight. The higher the virulence of the vaccine virus strain, the more severe damage it caused to the lymphocytes of the bursa of Fabricius. In SPF chickens, the haemagglutination inhibition (HI) titres induced by a Newcastle disease (ND) vaccine administered at day old decreased in inverse ratio to the virulence of the IBD vaccine strain, while in broiler chickens this was not observed. Despite the decrease of the HI titre, the level of protection did not decline, or did so only after the use of the 'hot' strain. SPF chickens immunized in ovo with a complex vaccine prepared from strain IBDV 2512 and IBD antibody showed the same protection against Newcastle disease as the broilers. In broiler chicken embryos immunized in ovo, only strain IBDV 2512 induced antibody production, and such chickens were protected against IBD at 3 weeks of age. The complex vaccine administered in ovo has been used successfully at farm hatcheries as well.  相似文献   

17.
J M Sharma 《Avian diseases》1985,29(4):1155-1169
Studies with specific-pathogen-free chickens revealed that chicks hatching from eggs inoculated at the 18th day of embryonation with infectious bursal disease (IBD) vaccine viruses of low virulence (isolates TC-IBDV and BVM-IBDV) developed antibody against IBD virus (IBDV) and resisted challenge with virulent IBDV at 3 weeks of age or older. Embryo vaccination did not adversely affect hatchability of chicks or survival of hatched chicks. Chicks embryonally vaccinated with TC-IBDV had transient histologic lesions in the bursa of Fabricius at hatch. Similar but milder lesions were also noted in chickens that received TC-IBDV at hatch. The level of protection following embryo vaccination with TC-IBDV and BVM-IBDV was similar to that following vaccination with the same vaccines at hatch. Vaccine viruses of moderate virulence (isolates BV-IBDV and 2512-IBDV) were not suitable as vaccines in embryos lacking maternal antibody to IBDV, because the vaccinated chicks developed acute IBD after hatch. Isolate 2512-IBDV was not pathogenic for embryos bearing maternal antibody to IBDV. Maternal antibody against IBDV interfered with efficacy of embryo vaccination with BVM-IBDV but not with 2512-IBDV. Embryo vaccination with a mixture of vaccines against IBD and Marek's disease resulted in protection of hatched chicks against challenge with virulent IBDV and Marek's disease virus.  相似文献   

18.
对利用IBDV地方野毒株所制备的囊毒和胚毒二价灭活油乳苗免疫效果进行了比较研究。结果表明,所制备的囊毒灭活苗免疫效果显著优于胚毒灭活苗和活疫苗,在接种后各个时期所产生的抗体效价均显著高于其它两种疫苗(P<0.01),最高AGP效价可达4.8log2;胚苗的免疫效果虽然差于囊苗,但明显优于活疫苗,当活苗免疫组鸡血清中已无可测性抗体时,胚苗组仍具有1.0log2的AGP效价。在攻毒试验中,囊苗组鸡对IBDV标准毒、两株野毒株的攻击均100%地获得了保护;胚苗组也均可获得80%的保护,这两种由地方毒株所制备的疫苗均未呈现出对不同毒株抵抗性的不同,而活苗组对不同毒株的攻击则分别只有53.3%、40%和46.7%的保护率,呈现出一定的差异性。  相似文献   

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