共查询到20条相似文献，搜索用时 234 毫秒
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对湖南地区某奶牛场隐性乳房炎发病牛病原菌感染和耐药情况进行分析.对该牛场连续5次送检的乳样进行分离纯化培养、16sRNA PCR测序、药敏试验以及耐药基因检测.结果显示:共分离出链球菌8株.基因测序鉴定显示乳房链球菌4株、停乳链球菌2株、A群链球菌2株;药敏试验显示8株链球菌对氨苄西林、头孢拉定、万古霉素高度敏感,对阿奇霉素、四环素中度敏感,对诺氟沙星、庆大霉素、链霉素不敏感;耐药基因检测显示大环内酯类耐药基因ermB、喹诺酮类耐药基因gyrA、ParC检出率最高,与细菌耐药谱相关性不强. 相似文献
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为鉴定驴精液中分离到的1株细菌的种类,采用形态学观察、绘制分离菌生长曲线和PCR扩增法对分离菌株进行了种类鉴定,并采用细菌药物敏感实验对该菌株进行了耐药性分析.结果表明,该菌在甘露醇高盐琼脂培养基上可形成边缘整齐、圆形、光滑湿润的不透明黄色菌落,革兰染色发现该菌为革兰阳性菌,呈葡萄串球菌;生长曲线分析结果显示,该菌在5... 相似文献
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《中国兽医杂志》2014,(6)
本试验旨在对沙门菌临床分离株进行鉴定及其耐药性进行研究。通过收集2012年2月-7月间具有典型沙门菌病病变特征的病例,无菌采集病死禽的肝脏、心、卵巢等脏器接种血清营养琼脂培养基,以麦康凯琼脂培养基纯化分离菌,并检测其生化特点,16S rDNA基因测序法快速鉴定分离菌株,然后进一步鉴定血清型;同时用15种抗菌药物进行药敏试验。结果显示,有15株细菌在麦康凯琼脂培养基上为无色、透明的菌落,能利用葡萄糖、甘露醇,不发酵乳糖、蔗糖,符合沙门菌的生化特性。同时,15株沙门菌的16S rDNA序列分析结果显示,分离株的同源性达到97%以上。15株沙门菌的血清学试验结果显示,有7株鼠伤寒沙门菌、5株伤寒沙门菌、2株肠炎沙门菌、1株鸡白痢沙门菌。药敏试验结果显示,分离细菌对链霉素、萘啶酸、强力霉素和美洛西林耐药率高。 相似文献
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为确定奶牛垫料中是否含有病原菌并深入了解垫料中优势生长菌的类型、耐药性及致病性等情况,本试验进行了细菌分离培养、革兰染色镜检、生化试验鉴定、16S rDNA序列分析及同源性比对、药敏试验、小鼠致病性试验。结果显示,分离菌在普通营养琼脂平板上形成圆形、表面光滑的白色菌落,血琼脂平板上形成黏稠、较大的白色菌落。革兰染色镜检结果显示,分离菌为革兰阳性,球型,呈葡萄串状排列,或单个散落。生化试验结果显示,分离菌对木糖、葡萄糖、麦芽糖、果糖、乳糖、硝酸盐还原等呈阳性反应,而蜜二糖、木糖醇、山梨醇、尿素、V-P反应等呈阴性反应。16S rDNA序列分析结果显示,扩增的16S rDNA序列长度为1 298 bp,与松鼠葡萄球菌的核苷酸同源性达99.85%~100%;系统进化树结果显示,分离菌与松鼠葡萄球菌处于同一分支。动物试验结果表明,分离菌株对试验小鼠有较强的致病性,以0.2 mL/只（7.9×108 CFU/mL）菌液的剂量接种小鼠,在48 h内死亡率为60%（3/5）。药敏特性分析结果显示,分离菌株对复方新诺明、氨苄西林等7种药物敏感,对克林霉素、头孢噻肟和头孢呋辛中度敏感,对青霉素、红霉素和林可霉素耐药。本研究为奶牛源松鼠葡萄球菌的分离鉴定及防控提供了参考依据。 相似文献
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本研究旨在从腹泻牦牛粪便中分离鉴定牛链球菌,并分析其溶血性、对小鼠的致病性及对抗菌药物的敏感性。将川西北阿坝州45份腹泻牦牛粪便于血平板上划线,37℃、5% CO2培养24 h分离细菌,经16S rRNA序列扩增测序和系统发育分析鉴定出14株牛链球菌,其中8株巴黎链球菌,6株解没食子酸链球菌巴氏亚种;9株呈α溶血,5株呈β溶血。分离鉴定的牦牛源巴黎链球菌和解没食子酸链球菌巴氏亚种能引起试验小鼠的轻度腹泻;药敏试验结果显示分离菌株对青霉素、头孢噻肟、万古霉素、乙酰螺旋霉素、环丙沙星、利福平、替考拉宁、氨苄西林和庆大霉素共9种抗生素高度敏感,对链霉素、卡那霉素、林可霉素、红霉素、四环素和克林霉素耐药率较高。本研究阐明了牦牛源链球菌的部分生物学特性,为该病的防控奠定了基础。 相似文献
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云南省某规模化肉牛养殖场2~6月龄犊牛持续出现轻度腹泻,粪便呈灰绿色水样。粪便样品经牛腺病毒(BAd V)3、7型、牛呼吸道合胞体病毒(BRSV)、牛疱疹病毒I型(BHV-I)、牛病毒性腹泻病毒(BVDV)、牛细小病毒(BPV)、牛冠状病毒(BCV)、牛轮状病毒(BRV)、隐孢子虫(Cryptosporidium parvum)real-time PCR及常规PCR检测均为阴性,将样品划线接种于血清琼脂、营养琼脂及麦康凯琼脂平板上37℃培养24 h,在血清琼脂及营养琼脂平板上生长出直径2~3 mm、稍突、圆形、湿润、灰白色、边缘整齐、不透明菌落,在麦康凯琼脂平板上菌落呈现粉红色。该分离株细菌经革兰氏染色镜检、细菌生化鉴定为大肠埃希氏菌,昆明小白鼠致病性实验显示致病力较强,实验组20只小白鼠接种后48 h内全部死亡,并从死亡小白鼠肝脏及心血成功分离出该株细菌。药敏试验结果显示该株细菌对环丙沙星、左氧氟沙星、氧氟沙星、头孢噻肟、头孢哌啶、头孢吡啶、头孢曲松钠高度敏感。 相似文献
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通过采样拭子采集患口腔炎的猫口腔黏膜、舌头、牙龈分泌物样本,分离到23株菌,经致病性试验证实有3种菌具有致病性。根据形态、生长特性和生化特性,11株被鉴定为葡萄球菌,9株被鉴定为链球菌,3株被鉴定为巴氏杆菌;从5只健康猫样本中分离到大肠杆菌5株。细菌分离结果表明,大肠杆菌为常在非致病菌,葡萄球菌、链球菌及巴氏杆菌为致病菌。药敏试验结果表明,分离的3种致病菌对头孢噻肟高度敏感。因此临床上治疗应首选头孢类抗生素。 相似文献
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ZU Fei DUAN Gang XIANG Xun LI Hao-xin YANG Zhi-yuan ZHU Qi DAI Fei-yan CHANG Hua 《中国畜牧兽医》2015,42(11):2902-2908
In this study,the dung samples of a sick cattle from an animal hospital were detected by bacteria cultivation.The bacteria were cultured by different differential media,and analyzed by biochemical test,16S rDNA gene was amplified by PCR,then sequenced and constructed phylogenetic tree,drug sensitivity test and growth curve were carried out.The results showed that the bacteria could grow smooth bulge,rounded edges,different size,different colors of the bacterial colonies in blood agar,chocolate agar,LB agar and so on,but could not grow in MRS agar and high salt mannitol agar.The results of lactose,maltose,mannitol and so on were negative,the results of glucose,simmon's citrate and urea were positive,this bacteria were identified as Streptococcus bovis by these preliminary results,and confirmed by phylogenetic tree construction.This isolate exhibited 99% nucleotide sequence identity with Streptococcus bovis RD09.The drug sensitivity test result of Streptococcus bovis showed that it was extremely sensitive to doxycycline and kanamycin,highly sensitive to erythromycin,sensitive to streptomycin,novobiocin,cotrimoxazole,ceftriaxone and gentamycin,resistant to chloramphenicol,cephalosporins cefradine and amoxicillin.The growth curve of Streptococcus bovis indicated that a rapid growth phase of the bacteria was from 2 to 23 h,the stationary phase was from 23 to 28 h,the decline phase was after 28 h.These experimental data provided the prerequisite for further study of Streptococcus bovis. 相似文献
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【目的】确定引起新疆石河子地区集约化牛场常发性肺炎的主要病原同时进行病原的体外药物敏感性分析。【方法】采集有典型咳嗽、流涕症状的牛鼻拭子10份和病死牛肺脏组织1份,用牛支原体特异性引物进行PCR检测,将检测为阳性的样本进行病原培养纯化,对纯化后的分离株菌落进行形态学观察、Dienes染色、生化试验及16S rRNA测序和进化分析,通过测定颜色变化单位(CCU)测定分离株生长曲线,并对分离株进行药物敏感性试验。【结果】PCR结果显示,10份鼻拭子中检测出7份牛支原体阳性样本,1份病死牛肺脏组织也检测为阳性;在涂有肺脏组织研磨液培养液的PPLO固体培养基上长出针尖状的菌落,纯化后分离株菌落形态为典型的煎蛋状;Dienes染色可见明显的深蓝色中心脐;生化试验结果显示,分离株不水解明胶、精氨酸、七叶苷,不发酵乳糖、葡萄糖和甘露醇,不分解尿素,可还原氯化三苯基四氮唑;16S rRNA测序结果显示,分离株与牛支原体国际标准株PG45相似性为99.7%,与国内牛支原体地方流行株XBY01、Ningxia-1、NM2012、Tibet-10的相似性最高,均为99.9%;生长曲线测定结果显示,分离株在培... 相似文献
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【目的】 探究引发浙江省某养殖场大黄鱼发病的病原及其致病性和耐药情况。【方法】 无菌条件下剖检发病大黄鱼，取其肝脏和肾脏组织，划线分离培养细菌，通过形态学观察、生理生化试验、16S rRNA基因序列分析鉴定分离菌的种属特性，通过人工感染和组织切片制备评价分离菌对大黄鱼的致病性，最后通过药敏纸片法检测分离菌的耐药性。【结果】 分离菌在培养基上外观为表面凸起、光滑和边缘整齐的黄色圆形菌落；革兰氏染色后光学显微镜下呈现出单个分散或成对分布的红色短棒状杆菌；该分离菌可在麦康凯琼脂上生长，可利用葡萄糖产酸；16S rRNA基因序列相似性分析及系统进化树显示，该分离菌与金黄杆菌相似性高达97.0%以上并聚为一类。动物回归试验结果显示，分离菌可引起大黄鱼的肝脏、肾脏和脾脏发生明显病变，半数致死量为6.32×104 CFU/mL，表明其对大黄鱼具有较强致病性；药敏结果显示，分离菌对阿米卡星和左氧氟沙星敏感，对庆大霉素、新霉素和红霉素中度敏感，对头孢克肟、氨苄西林、卡那霉素、呋喃唑酮等耐药。【结论】 本研究分离了大黄鱼源致病株金黄杆菌，并通过其致病性和耐药性研究为金黄杆菌引发水产动物发生病害的防控和治疗提供参考。 相似文献
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为探究鱼源蜡样芽孢杆菌的致病性和耐药性及指导科学用药,本试验采用形态学观察、生理生化特性分析、16S rDNA基因扩增及系统进化树构建等方法鉴定分离菌,人工感染试验确定分离菌的致病性,耐药基因检测和药敏试验确定菌株耐药性。结果显示,分离菌株形成边缘不规则、不光滑的乳白色菌落,为需氧型革兰氏阳性菌。葡萄糖、硝酸盐、明胶液化等生化反应为阳性,木糖、阿拉伯糖、甘露醇等生化反应为阴性。16S rDNA基因片段长度为1 457 bp,在系统发育树中,分离菌与蜡样芽孢杆菌RTR菌株亲缘关系最近,与蜡样芽孢杆菌的同源性均达到99%以上,从而综合判定分离菌株为蜡样芽孢杆菌。人工感染试验发现,分离菌株对黄颡鱼有一定的致病性。耐药基因检测结果显示,分离菌株中检测出Sul1和Sul2两种耐药基因。药敏试验发现,该菌株对庆大霉素、哌拉西林、米诺环素等敏感,对头孢哌酮中度敏感,对青霉素、头孢曲松、氨苄西林等耐药。本试验结果为有效防控蜡样芽孢杆菌感染的疾病提供了科学参考资料。 相似文献
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本研究旨在建立一种快速鉴定分枝杆菌的三重PCR方法,并比较分析其在临床检测中的可靠性。根据已发表的结核分枝杆菌、牛分枝杆菌和非洲分枝杆菌rv 3036c基因,结核分枝杆菌rv 1970f基因(RD7)和牛分枝杆菌pncA基因的序列,改造并设计合成了3对特异性扩增引物,建立了一种能对分枝杆菌样品进行初步鉴定的三重PCR方法。结果显示该方法可针对rv 3036c、rv 1970f和pncA基因分别扩增出大小为500、125和249 bp的目的片段,能特异性检测出结核分枝杆菌(500和125 bp两条带)和牛分枝杆菌(500和249 bp两条带),并可将结核分枝杆菌、牛分枝杆菌与其他分枝杆菌加以区分。本方法的检测灵敏度为50 pg/μL模板基因组DNA。对86株抗酸染色阳性菌进行三重PCR鉴定,鉴定结果与细菌16S rDNA和ITS序列测定结果一致,检测准确度为100%,优于生长特征和生化试验鉴定。 相似文献
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为了检测确定2019年5月河南某规模化猪场一栋保育仔猪发病猪群的病原,本研究从送检的发病猪关节液中分离获得1株细菌。通过细菌纯化培养、革兰氏染色、形态学观察及猪链球菌gdh基因PCR扩增,确定该分离菌株为猪链球菌。用猪链球菌分型引物对该菌株进行PCR扩增分型鉴定及软件比对分析,结果表明该分离菌株为猪链球菌14型,与猪链球菌JS14株（GenBank登录号：CP002465.1）同源性为100%。毒力基因检测结果表明,该菌株的同时携带有epf、mrp、sly、fbps、orf2毒力基因,属于高致病性菌株。小鼠致病性试验结果也证明该菌株是一株高致病性猪链球菌。药物敏感性试验结果显示,该菌株对β内酰胺类和喹诺酮类药物敏感,对氨基糖苷类、四环素类、大环内酯类和磺胺类高度耐药,表现出多重耐药现象。对该菌株进行5大类24种耐药基因检测,该菌株同时携带有blaTEM、aadA1、strA、strB、aacC2、aphA1、tet（B）、gyrA、parC、sul2耐药基因。该研究为后续进一步开展猪链球菌14型流行特点和致病机制研究奠定了基础,为猪链球菌14型临床防控提供了理论依据,同时具有重要的公共卫生意义。 相似文献
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LOU Zhong-zi LIU Cong-nuan JIA Wan-zhong ZHOU Ji-zhang YAN Hong-bin CAO Xiao-an LI Li LI Zhao-cai FU Bao-quan 《中国畜牧兽医》2016,43(1):8-15
This study was aimed to establish a triple PCR method to rapidly identify Mycobacterium species, and evaluate its testing reliability.Three pairs of primer that were respectively specific to rv 3036c, rv 1970f and pncA genes of Mycobacterium were designed to establish a triple PCR for preliminary identification of Mycobacterium tuberculosis(M.tuberculosis), Mycobacterium bovis(M.bovis) and other Mycobacterium spp.PCR products were the expected sizes of 500(rv3036c), 125(rv1970f) and 249 bp(pncA), and contained two DNA bands(500 and 125 bp) with M.tuberculosis DNA template, two DNA bands(500 and 249 bp) with M.bovis DNA template.No band or non-specific band appeared with Mycobacterium spp.except M.tuberculosis and M.bovis DNA templates.The sensitivity of the triple PCR was calculated to 50 pg/μL template of genomic DNA.86 acid-fast bacteria were detected by the triple PCR, 16S rDNA and ITS gene sequencing, growth test and biochemical test, and the results were consistent between triple PCR and 16S rDNA and ITS gene sequencing.The detecting accuracy of triple PCR was 100%, and higher than growth test and biochemical test. 相似文献
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为了解番鸭源鸭疫里默氏杆菌的耐药性,本研究对疑似鸭疫里默氏杆菌感染的发病番鸭进行细菌分离培养、革兰氏染色镜检、病鸭病原检测、生化试验、16S rRNA序列分析、PCR鉴定、药敏试验和耐药基因检测。细菌分离结果显示,分离菌在鲜血琼脂培养基上长出表面光滑、边缘整齐、有光泽、半透明的奶油状针尖大小菌落;革兰氏染色镜检呈革兰氏阴性短小杆菌,命名为GZQN201907。GZQN201907生化试验中尿素反应阳性,葡萄糖、麦芽糖、乳糖等生化反应呈阴性。其16S rRNA系统进化树与鸭疫里默氏杆菌处于同一分支;并且分离菌鸭疫里默氏杆菌OmpA基因PCR鉴定结果为阳性。其对头孢呋辛、红霉素、头孢他啶等18种抗菌药耐药,对羧苄西林和环丙沙星中度敏感,对新霉素和复方新诺明敏感。而耐药基因能检测出β-内酰胺类耐药基因VIM、TEM,四环素类耐药基因tetB,大环内酯类耐药基因ermB和ermF。药敏试验与耐药基因检测结果说明,GZQN201907对β-内酰胺类、四环素类、大环内酯类3类药物的耐药表型和耐药基因检测结果一致。动物回归试验中接种GZQN201907的雏鸭在72 h内全部死亡,而对照组雏鸭未出现任何症状,说明GZQN201907对雏鸭有致病力。试验成功分离到1株番鸭源鸭疫里默氏杆菌,为鸭疫里默氏杆菌病的防治奠定基础。 相似文献
19.
In order to understand the drug resistance of Riemerella anatipestifer from Muscovy duck,this study carried out bacterial isolation and culture,Gram staining microscopy,pathogen detection,biochemical test,16S rRNA sequence analysis,PCR identification,drug sensitivity test and drug resistance gene detection for Muscovy duck suspected of Riemerella anatipestifer infection.The results of bacterial isolation showed that on the blood agar medium,the isolated bacteria grew creamy needle tip size colonies with smooth surface,neat edge,luster and translucency.The Gram-negative bacillus brevis was detected by Gram-negative staining microscopy,and it was named GZQN201907.In the biochemical test of GZQN201907,urea reaction was positive,but glucose,maltose,lactose and other biochemical reactions were negative.The 16S rRNA phylogenetic tree was in the same branch as Riemerella anatipestifer.And the OmpA gene of PCR identification results of the isolated strain were positive.The drug sensitivity test was sensitive to 18 antibiotics including cefuroxime,erythromycin and ceftazidime,moderately sensitive to carboxypicillin and ciprofloxacin,and sensitive to neomycin and cotrimoxazole.And the resistance genes could detect the β-lactam resistance genes VIM and TEM,tetracycline resistance genes tetB,macrolide resistance genes ermB and ermF.The results of drug sensitivity test and drug resistance gene detection indicated that GZQN201907 showed the same resistance phenotype and gene detection results for β-lactam,tetracycline and macrolide.In the animal regression test,all the ducklings inoculated with GZQN201907 died within 72 h,while the control group showed no symptoms,indicating that GZQN201907 was virulent to the ducklings.One strain of Riemerella anatipestifer from Muscovy duck was successfully isolated,which laid a foundation for the prevention and treatment of Riemerella anatipestifer from muscovich. 相似文献
20.
QIAO Hongxing DING Yong ZHANG Liheng SONG Yuzhen DONG Qing BIAN Chuanzhou ZHAO Junqiang 《中国畜牧兽医》2022,49(2):435-442
【Objective】 The experiment was aimed to study the pathogenic bacteria of acute death of ducklings in a duck farm in Henan province and its phylogenetic status.【Method】 The liver and spleen of dead ducks in the diseased duck farm were collected.The bacterial morphology, Gram-staining, biochemical characteristics, sequence analysis of 16S rRNA gene, drug sensitivity test and pathogenicity test were carried out.【Results】 The isolated bacteria grew on blood agar medium with smooth, convex, milky white and α-hemolytic Gram-positive cocci.Biochemical test results showed that the isolated bacteria were positive for maltose, sucrose, raffinose, nitrate reduction reaction, MP-VP test, urea, lysine decarboxylase, ornithine decarboxylase and aescin, and negative for xylose, lactose, glucose, sorbitol, hydrogen sulfide, citrate, peptone, mannitol, phenylalanine and methyl red.The results of 16S rRNA gene sequencing and BLAST comparison results showed that the 16S rRNA gene sequence of the isolated strain was 94.8%-99.9% similar to that of Aerococcus viridans published on NCBI, and the similarity with strains Mnlv1, Mnlv2 and W66 was the highest, up to 99.9%.The similarity with strain GXBl-1 was the lowest, which was 94.8%.Phylogenetic tree analysis showed that the isolated bacteria belonged to the same genus and group on the same branch as strains of Aerococcus viridans FL09, 15MS and Mnlv2 etc., and had the closest genetic relationship with FL09.The results of drug sensitivity showed that the isolated strain was sensitive to fosfomycin, ampicillin and amoxicillin, moderately sensitive to penicillin, neomycin and amikacin, it was resistant to rifampicin, neomycin, bacitracin, erythromycin and enrofloxacin.The results of pathogenicity test showed that the strain was lethal and increased with the increase of inoculation concentration.【Conclusion】 This study determined that the pathogen causing the acute death of ducklings in a duck farm in Henan province was Aerococcus viridans, which provided a reference basis for clinical diagnosis and treatment of the diseases caused by this bacteria. 相似文献