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1.
将鸡传染性法代囊病病毒(IBDV)BX株抗原和IBDV高免血清按一定比例混合,初步制备了无菌的鸡传染性法氏囊病免疫复合物疫苗1~5(分别含有32、8、4、0.5及0.125单位IBDV中和抗体),用鸡传染性法氏囊病免疫复合物疫苗1~5及BX株活疫苗分别免疫1日龄低母源抗体水平雏鸡,免疫后9 d观察法氏囊病变,免疫后28 d采血测定IBDV中和抗体,并用强毒攻击,计算各组疫苗保护率。结果显示,免疫后9 d,复合物疫苗1~3免疫组鸡法氏囊正常,复合物疫苗4、5免疫组和BX株活疫苗免疫组分别有2/10、4/10和5/10的试验鸡法氏囊出现了病变;免疫后28 d,复合物疫苗1~5免疫组和BX株活疫苗免疫组IBDV中和抗体效价分别为8.34log2、9.60log2、9.21log2、7.88log2、9.50log2和9.12log2,攻毒保护率分别为90%、100%、100%、80%、90%和80%。试验结果表明鸡传染性法氏囊病免疫复合物疫苗2、3(分别含8、4单位IBDV中和抗体)免疫1日龄低母源抗体水平的雏鸡效果最好,攻毒保护率能达到100%。  相似文献   

2.
用鸡传染性法氏囊病免疫复合物疫苗和活疫苗分别免疫1日龄SPF雏鸡,免疫后9d观察法氏囊病变,免疫后14、21、28d对各组鸡采血测定IBDV中和抗体,21d及28d时用强毒攻击,计算各组疫苗保护率。结果表明,免疫后9d免疫复合物疫苗组鸡法囊正常,活疫苗组鸡法氏囊全部明显萎缩;免疫后14、21、28d,免疫复合物疫苗组鸡IBDV中和抗体分别为2.9±0.99、8.1±0.88和10.6±1.78,活疫苗组IBDV中和抗体分别为3.3±1.14、8.3±1.16和9.2±1.23,免疫后21d及28d疫苗免疫组均100%保护。试验证明免疫复合物疫苗比活疫苗更为安全,免疫复合物疫苗免疫SPF雏鸡产生比活苗更高的IBDV中和抗体,保护率达100%。  相似文献   

3.
为比较鸡传染性法氏囊病(IBD)免疫复合物疫苗与活疫苗免疫鸡法氏囊及外周血淋巴细胞(PBMC)中的病毒载量及免疫效果,本研究采用IBD免疫复合物疫苗和鸡传染性法氏囊病毒(IBDV)BX株活疫苗免疫1日龄SPF鸡,于免疫后7d、14d、21d、28d、35d、42d采用SYBRGreenI荧光定量PCR、ELISA方法及中和试验检测免疫鸡法氏囊和PBMC中IBDV载量和免疫鸡血清中IBDV抗体滴度,并攻毒,计算两种疫苗的保护率。结果显示,活疫苗免疫7d时IBDV在法氏囊和PBMC中的载量均比其它时间点的高,在14d时到达到最高,之后逐渐下降;两种疫苗刺激产生的IBDV抗体滴度随免疫时间的延长呈升高趋势;在免疫后7d攻毒两种疫苗的保护率均为0,其它时间的攻毒保护率均为100%。本研究结果表明,IBD免疫复合物疫苗与活疫苗免疫后病毒在鸡体内开始大量复制的时间并不相同,但免疫效果基本相同。本实验为IBD免疫复合物疫苗的研发提供实验依据。  相似文献   

4.
为评估鸡新城疫(ND)-传染性支气管炎(IB)-传染性法氏囊病(IBD)三联灭活疫苗对不同日龄和不同水平母源抗体雏鸡的免疫效力和持续期,本试验用该疫苗免疫7、14、21日龄SPF雏鸡和有母源抗体的普通雏鸡,免疫后采血测定ND血凝抑制抗体(HI Ab)、IB血凝抑制抗体(HI Ab)及IBD中和抗体(NA),并用传染性法氏囊病病毒(IBDV)强毒攻击。结果显示,7日龄SPF雏鸡免疫后21 d ND HI抗体、IB HI抗体及IBD中和抗体效价分别为7.9log2、6.9log2和14.1log2,SPF鸡日龄越大,抗体水平越高;28日龄SPF鸡免疫后3个月,0.3 mL免疫剂量组试验鸡ND HI、IB HI及IBD中和抗体效价分别达6.5log2、6.1log2和13.6log2,IBDV攻毒保护率均为100%(10/10);不同日龄普通雏鸡免疫效果与SPF鸡试验一致,抗体水平随鸡日龄增大而升高,IBD攻毒保护率也都达到100%(10/10)。试验结果证实,鸡新城疫-传染性支气管炎-传染性法氏囊病三联灭活疫苗可使7、14及21日龄SPF雏鸡和普通雏鸡产生良好的免疫力,对雏鸡的免疫期至少为3个月。  相似文献   

5.
为评估鸡新城疫（ND）-传染性支气管炎（IB）-传染性法氏囊病（IBD）三联灭活疫苗对不同日龄和不同水平母源抗体雏鸡的免疫效力和持续期,本试验用该疫苗免疫7、14、21日龄SPF雏鸡和有母源抗体的普通雏鸡,免疫后采血测定ND血凝抑制抗体（HI Ab）、IB血凝抑制抗体（HI Ab）及IBD中和抗体（NA）,并用传染性法氏囊病病毒（IBDV）强毒攻击。结果显示,7日龄SPF雏鸡免疫后21 d ND HI抗体、IB HI抗体及IBD中和抗体效价分别为7.9log2、6.9log2和14.1log2,SPF鸡日龄越大,抗体水平越高;28日龄SPF鸡免疫后3个月,0.3 mL免疫剂量组试验鸡ND HI、IB HI及IBD中和抗体效价分别达6.5log2、6.1log2和13.6log2,IBDV攻毒保护率均为100%（10/10）;不同日龄普通雏鸡免疫效果与SPF鸡试验一致,抗体水平随鸡日龄增大而升高,IBD攻毒保护率也都达到100%（10/10）。试验结果证实,鸡新城疫-传染性支气管炎-传染性法氏囊病三联灭活疫苗可使7、14及21日龄SPF雏鸡和普通雏鸡产生良好的免疫力,对雏鸡的免疫期至少为3个月。  相似文献   

6.
用鸡法氏囊病免疫复合疫苗(IBDV-Icx)和常规IBD活疫苗分别免疫1日龄商品鸡和SPF鸡,进行疫苗的安全性及免疫效力比较.免疫后8d检测,IBDV-Icx免疫组鸡的法氏囊未见明显萎缩,而传统弱毒疫苗免疫组鸡的法氏囊萎缩明显;免疫后28d各组用IBDV标准强毒株进行攻击,IBDV-Icx免疫组鸡的攻毒保护率均为10/10,常规IBD活疫苗对照组分别为8/10及9/10.免疫后3个月,IBDV-Icx免疫组血清抗体可达AGP1∶32,攻击强毒仍为10/10保护,而常规IBD活疫苗免疫后2个月抗体AGP为0,攻毒保护率为1/10,免疫后3个月时攻毒10/10发病.  相似文献   

7.
为了评估鸡新城疫(ND)-传染性法氏囊病(IBD)-禽流感(AI)(H9亚型)三联灭活疫苗对雏鸡的免疫效力,采用该疫苗分别免疫1、7、10、14、21日龄SPF雏鸡和带有母源抗体的普通雏鸡,免疫后21d~28d采血测定ND血凝抑制抗体(HIAb)、IBD中和抗体(NA)及AI(H9)HIAb抗体,并用传染性法氏囊病病毒(IBDV)强毒攻击。结果显示,免疫后各不同日龄免疫组SPF雏鸡的ND HIAb、IBD NA及AI(H9)HIAb滴度分别为7.9log2~8.3log2、13.9log2~15.8log2及8.0log2~8.9log2,IBDV攻毒均100%保护;而普通雏鸡免疫后的ND HIAb、IBD NA及AI(H9)HIAb滴度,1日龄免疫组分别为5.9log2、12.1log2和5.6log2,7、10、14、21日龄免疫组分别为6.1log2~7.0log2、12.6log2~14.5log2和6.0log2~7.5log2;IBDV攻毒,1日龄免疫组普通雏鸡保护率为60%,7、14及21日龄免疫组保护率均为100%;结果证实,SPF雏鸡所产生的免疫效力要高于带有母源抗体的普通雏鸡,1日龄普通雏鸡免疫所产生的免疫效力明显低于其他日龄。根据试验结果,推荐该疫苗对雏鸡的使用日龄为7日龄~14日龄。  相似文献   

8.
本研究用传染性法氏囊病活疫苗(Gt株)免疫3周龄SPF雏鸡,免疫两周后,分别用IBDV超强毒株HLJ-4株与Gx株进行攻毒,攻毒剂量为10ELD50/0.1ml,攻毒结果表明:传染性法氏囊活疫苗(Gt株)能抵抗HLJ-4和Gx的攻击,SPF鸡保护率达100%。  相似文献   

9.
丛华  侯军 《中国家禽》2006,28(20):15-17
用实验室研制的鸡新城疫(La Sota株),鸡传染性法氏囊病(NF8株)单苗和二联活疫苗分别免疫7日龄SPF鸡以及有母源抗体的雏鸡研究其免疫效力。结果表明:用二联活疫苗免疫SPF雏鸡后,NF8株与La Sota株间不产生明显的相互干扰作用,攻毒保护率与各自的单苗相比无明显差异。用二联活疫苗免疫有母源抗体的雏鸡后,只进行一次免疫,新城疫免疫效果较好,但法氏囊免疫效果稍差,不能完全保护;一免后10天再进行第二次加强免疫,其抗体水平、攻毒后的保护率与各自的单苗相比无明显差异,具有完全保护效力。  相似文献   

10.
《畜牧与兽医》2015,(5):7-11
为探讨鸡新城疫病毒(NDV)、传染性法氏囊病毒(IBDV)二联灭活疫苗在蛋雏鸡养殖中的实际应用效果进行本试验。用鸡新城疫、传染性法氏囊二联灭活疫苗及传染性法氏囊活疫苗以不同免疫程序免疫7~14日龄蛋雏鸡,定期监测NDV、IBDV抗体水平,并在雏鸡60日龄时进行攻毒保护试验。结果表明,各二联苗免疫组NDV抗体水平在免疫后14 d即达到NDV血清学最低有效保护价标准,并维持至雏鸡60日龄。免疫后28 d,灭活苗一次性免疫各组IBDV中和抗体水平显著高于活苗两次免疫组,琼扩抗体水平与活苗两次免疫组无明显差异。雏鸡60日龄时攻毒,各免疫组均100%保护,对照组无保护。以上结果说明该二联灭活疫苗可替代IBDV活毒疫苗用于7~14日龄商品雏鸡免疫,免疫期至少为60d。  相似文献   

11.
One-day-old moderate maternal antibody Jinghong chickens and SPF chickens were vaccinated with infectious bursal disease (IBD) immune complex (IC) vaccine and BX strain live vaccine,respectively,pathological changes of bursa of fabricius in chickens were observed at the 9th day after immunization.On the 28th day after immunization,blood samples were taken and the IBDV neutralizing antibody were tested,meanwhile experimental chickens were challenged with high virulent IBDV,the protective rates of experimental groups were calculated.The results showed that at the 9th day after immunization,the bursa of fabricius in SPF and Jinghong chickens were normal in IC vaccine group,however atrophic changes of bursa of fabricius in BX strain live vaccine group were shown.At the 28th day after immunization,the IBDV neutralizing antibody of IC vaccine group and BX strain live vaccine group in SPF and Jinghong chickens were 9.2log2,8.5log2,7.2log2 and 4.4log2,respectively,while 100% protection rates were provided.The results provided that IC vaccine had better immunity effect on one-day-old moderate maternal antibody chicken,high levels of neutralizing antibodies against IBDV were produced and protection rates were 100%.  相似文献   

12.
Five kinds of infectious bursal disease (IBD) immune complex (IC) vaccines were prepared with infectious bursal disease virus (IBDV) BX strain mixed with IBDV hyperimmune serum according to a certain proportion (containing 32, 8, 4, 0.5 and 0.125 units IBDV neutralizing antibody, respectively).One-day old low maternal antibody chickens were vaccinated with IBD IC vaccines 1 to 5 and BX strain live vaccine, respectively, pathological changes of the bursa of fabricius in chickens were observed at the 9th day after immunization.On the day of 28 after immunization, blood samples were taken and the IBDV neutralizing antibody were tested, meanwhile all experimental chickens were challenged with high virulent IBDV, the protective rates of vaccines were calculated.The results showed that at the 9th day after immunization, the bursa of fabricius were normal in IC vaccine 1, 2 and 3 groups, however 2/10, 4/10 and 5/10 of pathological changes of the bursa of fabricius in IC vaccine 4, 5 groups and BX strain vaccine group, respectively.At the 28th day after immunization, the IBDV neutralizing antibodies in IC vaccine 1, 2, 3, 4, 5 groups and BX strain vaccine group were 8.34log2, 9.60log2, 9.21log2, 7.88log2, 9.50log2 and 9.12log2, while 90%, 100%, 100%, 80%, 90% and 80% protection rates were provided, respectively.The results showed that IBD IC vaccines 2 and 3 (containing 8, 4 units IBDV neutralizing antibody, respectively) had the best immunity effect on one-day old low maternal antibody chickens, protection rates were both 100%.  相似文献   

13.
The appearance of very virulent strains of infectious bursal disease (IBD) virus at the end of the 1980s made it necessary to develop more effective immunization procedures. To facilitate this, the immunogenicity and the immunosuppressive effect of a mild (G-87), an intermediate (LIBD) and an intermediate-plus (IBDV 2512) IBDV strain were tested after the in ovo inoculation of 18-day-old SPF and broiler chicken embryos. It was established that no noteworthy difference existed between the immunized and the control embryos in hatching rate and hatching weight. The higher the virulence of the vaccine virus strain, the more severe damage it caused to the lymphocytes of the bursa of Fabricius. In SPF chickens, the haemagglutination inhibition (HI) titres induced by a Newcastle disease (ND) vaccine administered at day old decreased in inverse ratio to the virulence of the IBD vaccine strain, while in broiler chickens this was not observed. Despite the decrease of the HI titre, the level of protection did not decline, or did so only after the use of the 'hot' strain. SPF chickens immunized in ovo with a complex vaccine prepared from strain IBDV 2512 and IBD antibody showed the same protection against Newcastle disease as the broilers. In broiler chicken embryos immunized in ovo, only strain IBDV 2512 induced antibody production, and such chickens were protected against IBD at 3 weeks of age. The complex vaccine administered in ovo has been used successfully at farm hatcheries as well.  相似文献   

14.
1-day-old SPF chickens and commercial Jingfen chickens were vaccinated with IBD immune complex(IC) vaccine, NDV La Sota vaccine were inoculated simultaneously every one week and every two weeks.NDV La Sota immunization alone was as the control group.At the 2nd, 3rd, 4th and 5th week post inoculation, blood samples were taken and the ND HI antibody were tested.Experimental chickens were challenged with high virulent NDV at the 5th week post inoculation, the protective rate of each group was calculated.The results showed the ND HI antibody were not significant different in the combined immunization of IBD IC vaccine priming and NDV La Sota vaccine boost and NDV La Sota vaccine alone immunization (P>0.05).The results indicated that IBD IC vaccine has no immunosuppression on NDV La Sota vaccine in SPF chickens and commercial Jingfen chickens.  相似文献   

15.
The evolution of very virulent (vv) infectious bursal disease virus (IBDV) has led to significant economic losses in many poultry-producing areas. Despite vigorous vaccination strategies, IBDV has been difficult to control. The protective efficacy of IBDV vaccines is traditionally evaluated in specific pathogen-free (SPF) chickens. But under field conditions, residual maternal antibody (mAb) levels may interfere with vaccine efficacy. In this study, commercial broilers with various levels of maternally derived antibodies were vaccinated with IBDV vaccines of different virulence (vaccines 1-3, intermediate; vaccine 4, intermediate plus). At an average maternal virus-neutralizing antibody (mAb) level of log2 10.8 (range 7.6-11.6) at day of vaccination, only the intermediate plus vaccine induced IBDV antibodies after 18 days, while the other intermediate vaccines did not. At average mAb levels of log2 6.7 (range 5.6-8.6) at day of vaccination, all vaccines induced circulating antibodies, although the onset of antibody production differed significantly between strains (P < 0.05). While the intermediate plus vaccine induced enzyme-linked immunosorbent assay antibody levels already at 14 days postvaccination (PV), the intermediate vaccines induced significant antibody levels 28 (vaccines 1, 2) and 35 (vaccine 3) days PV. The time of IBDV antibody induction correlated with the onset of bursa lesions. The severity of lesions was comparable between vaccines 1, 3, and 4 (lesion score 4), while vaccine 2 induce only mild lesions of score 1 in 23% of the tested birds. Despite the induction of antibodies, none of the tested vaccines fully protected against challenge with vvIBDV. All challenged birds had either significantly higher bursal lesion scores or a higher IBDV antigen load in the bursa or sometimes both in comparison with nonchallenged birds (P < 0.05). Our study demonstrates that the evaluation of IBDV-vaccine efficacy is difficult in commercial broilers. For the first time, it was shown that the onset of bursa lesions and recovery of IBDV-vaccinated broilers is delayed in the presence of mAb in comparison with SPF chickens but not suppressed as previously assumed. At the time of challenge, vaccinated birds may still have significant bursa lesions and may lack target cells for IBDV-challenge virus. To be able to evaluate vaccine efficacy in commercial broilers, parameters such as intrabursal IBDV-antigen load should also be considered in conjunction with bursa lesion scores.  相似文献   

16.
Different infectious bursal disease virus (IBDV) live vaccines (intermediate, intermediate plus) were compared for their immunosuppressive abilities in specific-pathogen-free (SPF) layer-type chickens or commercial broilers. The Newcastle disease virus (NDV) vaccination model was applied to determine not only IBDV-induced immunosuppression but also bilateral effects between IBDV and NDV. None of the IBDV vaccines abrogated NDV vaccine-induced protection. All NDV-vaccinated SPF layers and broilers were protected against NDV challenge independent of circulating NDV antibody levels. Sustained suppression of NDV antibody development was observed in SPF layers, which had received the intermediate plus IBDV vaccine. We observed a temporary suppression of NDV antibody development in broilers vaccinated with one of the intermediate, as well as the intermediate plus, IBDV vaccines. Different genetic backgrounds, ages, and residual maternal antibodies might have influenced the pathogenesis of IBDV in the different types of chickens. Temporary suppression of NDV antibody response in broilers was only seen if the NDV vaccine was administered before and not, as it was speculated previously, at the time the peak of IBDV-induced bursa lesions was detected. For the first time, we have demonstrated that the NDV vaccine had an interfering effect with the pathogenesis of the intermediate as well as the intermediate plus IBDV vaccine. NDV vaccination enhanced the incidence of IBDV bursa lesions and IBDV antibody development. This observation indicates that this bilateral effect of an IBDV and NDV vaccination should be considered in the field and could have consequences for the performance of broiler flocks.  相似文献   

17.
为了选出法氏囊疫苗免疫效果评估的合适方法,本研究使用酶联免疫吸附试验(ELISA)、琼脂糖扩散试验(AGP)和中和抗体检测3种方法对6种法氏囊灭活疫苗免疫后28 d的抗体水平进行评估,同时,对采血鸡进行攻毒,攻毒后4 d剖检所有鸡,观察法氏囊病变,评估3种抗体水平与攻毒保护之间的相关性;结果显示,ELISA和AGP方法检测免后血清结果相对较高,中和试验方法检测免后抗体值较低;ELISA和AGP方法检测疫苗免后抗体水平与攻毒保护结果基本一致,使用中和试验方法检测免后抗体与攻毒保护相关性较差;建议在实际生产中使用ELISA和AGP方法进行法氏囊疫苗免疫效果的评估。  相似文献   

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