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2019年我国新疆首次发生牛结节性皮肤病,到目前为止该病已经在我国多个省份发生呈现流行趋势,该病主要临床表现为皮肤结节,主要特征是淋巴结肿大.牛结节性皮肤病的发生率在3％～85％,死亡率较低,在3％～6％,但有其他疫病混合感染时,死亡率升高会达到45％～75％,该病目前给我国养牛业带来了极大的危害.本文对牛结节性皮肤病的流行特点趋势,临床症状以及该病的预防控制等方面进行综合阐述,为预防牛结节性皮肤病的发生,减少该病对我国牛养殖产业带来的危害,提供理论基础. 相似文献
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牛结节性皮肤病(LSD)是由山羊痘病毒属结节性皮肤病病毒引起的一种高度传染性动物疫病.感染该病的牛会出现高热、皮肤结节等症状.该病的致死率较高,感染后可造成奶牛产奶量下降、母牛流产,对养牛业造成严重的经济损失.本文归纳总结了牛结节性皮肤病的病因、临床症状、流行特点、诊断治疗及防控措施,以期更好的预控牛结节性皮肤病,减少我国养殖业经济损失. 相似文献
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2013～2016年,全球共有60个国家报告发生牛结节性皮肤病(Lumpy skin disease,LSD)疫情。其中,非洲多数国家仍呈地方流行性,在中东地区进一步蔓延,传入伊拉克、伊朗等国家,并再次出现跨湖传播,传入部分亚欧交界国家和欧洲国家~([1])。2014～2018年,共12个欧洲国家和7个亚洲国家向OIE报告了6 106起牛结节性皮肤病疫情。2018年,疫情已经扩散至俄罗斯和哈萨克斯坦,进一步向东移动,接近我国新疆地区,传入我国的风险不断加大,有可能在未来一段时间内传入我国,从而对我国的养牛行业带来严重威胁~([2])。文章对牛结节性皮肤病进行了简要介绍,并阐述了目前针对该病的风险控制方法,为LSD的防控提供技术基础。 相似文献
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牛结节性皮肤病俗称牛疙瘩病,是由羊痘病毒属牛结节性皮肤病病毒感染引起的一种急性、亚急性或慢性传染病,在世界部分国家或地区呈地方流行.由于牛结节性皮肤病引起牛的发热、消瘦、淋巴结肿大、皮肤与黏膜的局部呈疙瘩性结节,造成奶牛和肉牛生产性能明显降低,且无特效的治疗药物,因此被我国列为二类动物疫病.本文对牛结节性皮肤病的诊断、... 相似文献
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牛结节性皮肤病是由牛结节性皮肤病病毒(LSDV)引起的一种牛全身性感染疫病,以皮肤出现结节为典型临床特征,严重影响着养牛业的发展,是世界动物卫生组织要求法定报告的动物传染病,我国将其列为二类动物传染病进行管理.
1 国内流行现状
2019年8月,新疆伊犁州发生了LSD疫情,是我国首次确诊的LSD疫情,经流行病学调查,认为经虫媒传入的风险最高.2020年以来,我国福建长汀、江西赣州、广东潮州、安徽黄山、浙江金华等地先后确诊了6起牛结节性皮肤病疫情,各地采取了积极有效的防控措施,防止了疫情的扩散和蔓延.该病的散发流行对我国的奶牛和肉牛养殖造成了严重的不良影响. 相似文献
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魏春梅 《河南畜牧兽医(综合版)》2019,(10):24-25
在我国新疆边境,哈萨克斯坦国流行着一种牛结节性皮肤病,是一种病毒性传染病,能通过蚊蝇等节肢昆虫传播,严重损坏牛的皮肤,影响皮张加工与销售.在与其相邻的我国新疆维吾尔地区养殖密度非常大,2019年8月12日,经中国动物卫生与流行病学中心国家外来动物疫病研究中心确诊,新疆维吾尔自治区伊犁州发生牛结节性皮肤病疫情,这是我国首次确诊发生该病.后经当地畜牧兽医部门排查,截至目前,又在伊犁州察布查尔县、霍城县、伊宁市发现病牛218头,死亡1头. 相似文献
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《畜牧兽医科技信息》2021,(7)
正牛结节性皮肤病是由痘病毒科山羊痘病毒属牛结节性皮肤病病毒引起的牛全身性感染疫病。世界动物卫生组织(OIE)将其列为法定报告的动物疫病。我国暂将其作为二类动物疫病管理,该病可对养牛业造成巨大经济损失。2019年8月首次传入我国新疆伊犁,根据农业农村部畜牧兽医局通报,截止2020年7月我国已在5省6市发生6起牛结节性皮肤病疫情。虽然,目前辽宁省并未发现牛结节性皮肤病,但根据该病传播特点,不排除该病零散发生的可能,仍需畜牧兽医部门加强认识,积极应对可能发生的疫情。 相似文献
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娄金艳 《畜牧兽医科技信息》2023,(7):151-153
牛结节性皮肤病是由牛结节性皮肤病病毒（LSDV）而引起的传染性皮肤病,2019年在我国新疆伊犁州首次确诊。近几年我国也偶有暴发,对养牛业危害较大。该病是OIE要求通报的疫病,我国将其列为二类动物疫病。本文对牛结节性皮肤病的流行特点、临床表现、病理变化和诊断措施进行了介绍和分析,并分享了有效的综合性防控措施,供参考。 相似文献
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Payman Sameea Yousefi Bahram Dalir-Naghadeh Karim Mardani Ghader Jalilzadeh-Amin 《Tropical animal health and production》2018,50(8):1851-1858
Lumpy skin disease (LSD) is a devastating viral disease of cattle which has recently spread from Africa into the countries of the Middle East. The aim of the present study was to investigate the relationships among lumpy skin disease viruses (LSDV) isolated from different regions of Iran and the origin and spread of these viruses. In this study, a total of 234 blood samples from clinically affected animals from four provinces in the northwest of Iran were screened for LSDV using polymerase chain reaction (PCR). From 80 positive samples for LSDV detected by PCR, the partial P32 gene (759 bp) of 12 isolates were sequenced and phylogenetically analyzed. LSD viruses were grouped in three subclusters with an overall 97.1–100% nucleotide identity. LSDVs isolated from Gilan showed lowest nucleotide identity with the other LSDVs. Four isolates of LSDV including KO-1, EA-1, EA-3, and WA-3 showed 100% similarity with each other and also with the Neethling strain. Phylogenetic analysis indicated that the identified LSDVs were closely related to each other and had high-sequence homology with other LSDV isolates from Africa. It was concluded that LSD outbreak probably occurred in the northwest of Iran by LSDVs entering the country from Iraq and P32 nucleotide sequence information obtained in the present study is a valuable resource in understanding the genetic nature and molecular epidemiology of local LSDV isolates which can be used for future vaccine development based on the circulating strains in the region. 相似文献
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Lumpy skin disease (LSD) is an endemic highly infectious viral disease affecting cattle in Egypt. This study aimed to identify and characterize the LSD virus (LSDV) outbreaks in Egypt between 2016 and 2018 and to determine the role of Egyptian buffaloes in the epidemiology of LSD. A total of 44 skin biopsies (41 from cattle and 3 from buffaloes) and 31 blood samples from asymptomatic buffaloes in contact with clinically infected cattle were collected from 7 Egyptian governorates and tested by real-time (rt)-PCR. The positive samples were further isolated, and the isolates were analyzed by conventional PCR to amplify the LSDV001 and LSDV002 genes; three isolates were sequenced, and the phylogenetic tree was constructed. In addition, 198 serum samples (102 from cattle and 96 from contact buffaloes) were examined using ELISA. Out of 44 skin nodules analyzed by rt-PCR, 31 (70.45 %) were positive while, non of the buffalo samples were positive. Out of 31 positive rt-PCR samples, LSDV was isolated on CAM (n=19; 61.29%) and MDBK cell culture. The virus isolates were confirmed by conventional PCR where 1237 bp product size was successfully amplified. The phylogenetic analysis of LSDV002 gene revealed that three sequenced LSDV isolates were identical to each other and to LSDV isolates from different countries in Africa, Asia, and Europe with 99–100 % identity. ELISA analyses showed seroreactivity of LSDV in Egyptian cattle and buffaloes. In conclusion, the Egyptian water buffalo serves as an accidental non-adapted host for the disease and this point requires more deep investigation. In addition, the current vaccine strategy should be re-evaluated for more coverage and effectiveness. 相似文献
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Lumpy skin disease (LSD) is an infectious viral disease affecting cattle, cause severe economic losses. In the last few years, the disease is widely distributed in many countries in the Middle East, including Egypt. This study aimed to determine the genetic character of LSDV circulating in some governorates in Egypt and its relation with other LSDVs and vaccine strains in GenBank. A total of 50 skin nodules and 50 blood samples were collected from clinically affected cattle to detect LSDV using PCR targeting the P32 gene. The positive samples were characterized using PCR targeting the GPCR gene. The amplified products of four samples detected in the skin nodule of cattle from Alexandria and Kafr ElSheikh governorates were sequenced, and the phylogenetic tree was constructed. Out of 100 analyzed samples, 70 samples were positive for LSDV by PCR assay. In addition, the sequence alignment of the obtained LSDV strains detected in the Alexandria governorate showed high similarity to the LSDV genome (MN995838). In contrast, LSDV strains from Kafr ElSheikh governorate revealed high similarity and the previous Egyptian strain (MG970343), which was isolated from cattle in Sharkia governorate in 2016. Also, the phylogenetic analysis confirmed that one of the LSDV strain (LC601598) from Alexandria is closely related to the LSDV of Menofia/Egypt/2019 (MN271722), while another one (LC601597) is closely related to vaccine strains of LSDV. Moreover, the LSDV strains from Kafr Elsheikh showed closely related to previous LSDV strains isolated from Menofia (MG970343) and Dakahlia (KP071936) governorates and clustered with other LSDV strains in a distinct cluster. This information is for understanding the epidemiology of LSDV and supporting the establishment of an efficient control program for the disease. 相似文献
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2020年11月，山东滨州市、东营市引进牛皮肤出现自限性皮肤结节、损伤以及结痂等症状，疑似发生牛结节性皮肤病（lumpy skin disease，LSD）。为确诊两地疫情及了解病原的遗传演化关系，利用荧光定量PCR方法进行诊断，以PCR方法扩增GCPR基因并进行核苷酸比对分析和遗传演化分析。检测结果显示，采集的样品中检测到牛结节性皮肤病病毒（lumpy skin disease virus，LSDV），两地疫情确诊为LSD疫情。GCPR基因核苷酸比对结果显示，China/SDBinzhou/2020、China/SDDongying/2020 GCPR基因存在12个核苷酸的插入，与疫苗毒株Neethling vaccine LW 1959株、Neethling-LSD vaccine-OBP株以及俄罗斯发现的疫苗样毒株Saratov株在GCPR基因的核苷酸插入序列相同，具备疫苗样毒株的特征。系统发育分析结果表明，China/SDBinzhou/2020、China/SDDongying/2020 GCPR基因与我国新疆发现的LSDV毒株GCPR基因处同一小分支中，亲缘关系较近。同时，我国LSDV毒株与Neethling vaccine LW 1959株、Neethling-LSD vaccine-OBP株以及Saratov株等共处于一大分支中。综上，确诊滨州市、东营市两地疫情为LSD疫情，这是山东省首次确诊输入性LSD疫情。 相似文献
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The isolation of lumpy skin disease virus and bovine herpesvirus-4 from cattle in Egypt 总被引：1，自引：0，他引：1
J A House T M Wilson S el Nakashly I A Karim I Ismail N el Danaf A M Moussa N N Ayoub 《Journal of veterinary diagnostic investigation》1990,2(2):111-115
Lumpy skin disease (LSD) virus (LSDV) was isolated for the first time from cattle in Egypt in 2 disease outbreaks. Bovine herpesvirus-4 (BHV-4) and LSDV were detected in a pooled sample from the first outbreak (Suez). Only LSDV was isolated from the second outbreak (Ismalia). The capripoxviruses were identified as LSDV by neutralization with specific antiserum and by their ability to produce generalized LSD in experimentally inoculated cattle. 相似文献
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为原核表达牛疙瘩皮肤病病毒(LDSV) ORF132并对该基因进行生物信息学分析,本研究采用PCR扩增LSDV的ORF132基因,将其亚克隆至pGEX-6p-1载体中,构建重组质粒pGEX-LSDV-ORF132.生物信息学分析表明,LSDV的ORF132与Neethling vaccine LW1959株LW132、NI-2490株LSDV132和NW-LW株LD132的相应推导氨基酸同源性分别为100％、100％和94.4％,与山羊痘病毒、绵羊痘病毒相应推导氨基酸的同源性为20.7 ％～36％.SDS-PAGE和western blot分析表明,pGEX-LSDV-ORF 132在大肠杆菌BL21中经IPTG诱导主要以包涵体形式存在,其重组蛋白大小约为48.95ku,并且与特异性抗体具有抗原反应活性. 相似文献
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CH Annandale PC Irons VP Bagla UI Osuagwuh EH Venter 《Reproduction in domestic animals》2010,45(2):250-255
The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell‐rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell‐rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate. 相似文献