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云南毛脚鸡是云南地方品种资源之一,为了解毛脚鸡感染肠道寄生虫的情况,随机采集新鲜粪样276份,采用沉淀法和漂浮法检查,并进行虫卵计数。结果：45份检出线虫卵,检出率16．3％,克粪便虫卵数3000—12000个;43份检出吸虫卵。检出率为15．58％,克粪便虫卵数8～13个;23份检出球虫卵囊,检出率为8．33％,克粪便卵囊数16400～26000个。所有阳性粪样均存在混合感染情况。结果表明：毛脚鸡感染吸虫和线虫较为普遍,感染球虫强度较高,为保护该品种资源,应该加强对寄生虫病的防治。 相似文献
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摘要为了解某规模化种羊场寄生虫感染情况,用寄生虫学剖解法检查2只体形衰弱的羊只,收集和鉴定虫体;随机直肠采集34只羊的粪样,任意选择圈舍、活动场和粪堆分别取样12、8和8份,用饱和食盐水漂浮法检查虫卵或卵囊,根据形态学鉴定虫种。结果发现：寄生虫学剖解法分别检出6765条和246条线虫,形态学鉴定为捻转血矛线虫;34份粪样中检出虫卵1—363枚,有27份检出（79．4％）;圈舍、活动场和粪堆分别检出1-21枚、4--26枚和1～4枚,检出率58．3％、100％和50．0％。表明该规模种羊场羊只感染率高,部分羊只感染强度特别高;圈舍、活动场和粪堆检出虫卵,表明环境已被虫卵严重污染,容易造成重复感染和交叉感染。 相似文献
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《养殖与饲料．饲料世界》2021,(10)
为了解云南省武定县乡镇羊的消化道寄生虫感染情况,从武定县的插甸镇某大耳黄羊羊场和猫街镇、狮山镇和田心乡的散养户中采集新鲜羊粪样共222份,采用饱和盐水漂浮法检测寄生虫。结果显示:样品线虫卵和球虫卵囊的总检出率为93.69%,其中线虫卵阳性率为81.98%、球虫卵囊阳性率为33.78%、线虫和球虫的混合感染率为22.07%;从不同采样点来看,插甸镇某大耳黄羊场的线虫卵和球虫卵囊检出率为91.30%,猫街镇的线虫卵和球虫卵囊检出率为93.15%,狮山镇的线虫卵和球虫卵囊检出率为100%,田心乡的线虫卵和球虫卵囊检出率为93.15%。从不同养殖方式来看,规模化养殖场的线虫卵和球虫卵囊检出率为91.30%、散养户的线虫卵和球虫卵囊检出率为94.32%。不同乡镇以及不同养殖方式的羊肠道寄生虫感染差异均不显著。结果表明:云南武定县部分乡镇羊肠道寄生虫感染普遍,且有混合感染。建议进行定期驱虫,加强饲养环境监控,提高科学饲养水平。 相似文献
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2011年4月-2012年3月,通过对北京市房山区、大兴区、怀柔区、通州区和密云县的奶牛养殖进行现场调研,采集新鲜粪样3000份,用饱和盐水漂浮法和水洗沉淀法对粪样进行了定性检查,用麦克马斯特计数法对线虫卵和球虫卵囊进行了定量检查。在粪样中没有检出吸虫卵;线虫卵感染率为6.37%,感染强度(EPG)在100～750之间,平均感染强度为206,并在秋季出现一个高峰;球虫卵囊感染率为46.03%,感染强度(OPG)在100～7250之间,平均感染强度为748,并在夏季出现一个高峰。结果表明,北京地区奶牛消化道寄生虫中,球虫卵囊阳性率较高,线虫卵阳性率相对较低,没有吸虫卵。有一定的季节性动态变化。 相似文献
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北京地区奶牛寄生虫感染情况的调查 总被引：1，自引：0，他引：1
为了了解北京地区奶牛胃肠道寄生虫感染情况,采集北京地区成年牛和犊牛的粪样,用饱和盐水漂浮法、水洗沉淀法对粪样进行了定性检查,用麦克马斯特计数法对线虫卵和球虫卵囊进行了定量检查。结果发现,北京地区成年奶牛和犊牛粪样中检出的主要是线虫卵和球虫卵囊,未检出吸虫卵。成年奶牛粪样中线虫卵的阳性率为14.64%,平均EPG值范围为190~853,并在5~9月份出现一个比较明显的高峰期;球虫卵囊阳性率为49.15%,平均OPG值范围为125~346,高峰出现在5~11月份。犊牛粪样中线虫卵的阳性率为24.56%,平均EPG值范围为305~1 053,并在5~11月份出现一个比较明显的高峰期;粪样中球虫卵囊阳性率为65.02%,平均OPG值范围为2 540~7 648,并在7月份出现一个高峰。根据调查结果,对北京地区奶牛寄生虫病防治提出了建议。 相似文献
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为了解大理某奶水牛场消化道寄生虫感染情况,提供防制建议,随机采集引进牛只、杂交1代、杂交2代和杂交3代新鲜粪样44份,进行漂浮法和沉淀法检查寄生虫,结果表明,总寄生虫检出数22份,总检出率为50.0%;18份检出吸虫卵,检出率为40.9%;7份检出线虫卵,检出率为15.9%,其中2份检出特异性蛔虫卵,占4.5%;3份同时检出吸虫卵和线虫卵,占6.8%。杂交3代的线虫检出率最高,杂交1代和杂交2代的吸虫卵检出率较高。建议采取重视寄生虫病的监测和诊断等综合防制措施。 相似文献
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为了解四川某奶牛场奶牛寄生虫感染情况,进而制订合理有效的防治措施,本调查对该场奶牛粪样做了寄生虫检查。采集了犊牛、育成牛、泌乳牛的新鲜粪样共90份,分别采用饱和盐水漂浮法、离心沉淀法和斯陶尔氏计数法对粪样中虫卵进行定性检查和定量检查。结果显示,在粪样中检出了球虫卵囊、线虫卵、吸虫卵和绦虫卵,其中球虫卵囊的阳性率为14.44%(13/90),线虫卵的阳性率为57.78%(52/90),绦虫卵和吸虫卵只在泌乳牛的粪样中检出,泌乳牛阳性率分别为2%(1/50)、12%(6/50)。该奶牛场混合感染率为33.33%(18/54)。结果表明,该奶牛场寄生虫感染普遍,总感染率为60%(54/90),主要为线虫,其次是球虫和吸虫,混合感染情况普遍。 相似文献
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The aim of the study was to assess whether the hair of stray and domestic dogs in Egypt was contaminated with the eggs of the zoonotic parasite Toxocara canis, and also to identify risk factors for T. canis for contamination. Paired samples of hair and feces were collected from 53 stray and 47 domestic dogs, and hair samples were obtained from a further 11 stray and 9 domestic dogs. All samples were examined to identify T. canis eggs and, if eggs were found, their maturation stage. Eggs were identified in 26.6% of stray and 10.7% of domestic dog's hair samples. A significantly increased risk of embryonated T. canis eggs in hair samples was found in stray dogs (p=0.04), stray dogs had 3.18 (95% CI: 1.04-9.74) times the odds of having T. canis eggs present compared with domestic dogs. There was also a significant difference (p=0.02) between the mean quantity of eggs per gram in stray (77.6±6.54) and domestic (48.7±6.65) dog's hair. Fecal examination found a T. canis egg prevalence of 35.8% and 21.3% in stray and domestic dogs, respectively. As no domestic dogs which were positive from hair samples had negative fecal samples, this indicates that the presence of T. canis eggs in hair is probably due to self contamination. Two stray dogs had positive hair samples but negative fecal samples indicating that contamination may also be environmental. As both non-embryonated and embryonated T. canis eggs were found in the hair of domestic dogs, direct contact with dogs may be a potential risk factor for transmission of T. canis eggs to humans. 相似文献
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Gennari SM Yai LE D'Auria SN Cardoso SM Kwok OC Jenkins MC Dubey JP 《Veterinary parasitology》2002,106(2):177-179
Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete N. caninum oocysts. In order to understand the prevalence of N. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of S?o Paulo, Brazil were assayed for antibodies to N. caninum. Sera were examined by the Neospora agglutination test (NAT) using mouse-derived tachyzoites. Antibodies (> or =1:25) to N. caninum were found in nearly 10% (49/500) of owned dogs and in 25% (151/611) of stray dogs. NAT titers for owned dogs were 1:25 in 28 (5.6%) dogs, 1:50 in 20 (4%) dogs, and > or =1:500 in 1 (0.2%) dog. NAT titers for stray dogs were 1:25 in 79 (12.9%) dogs, 1:50 in 68 (11.1%) dogs, and > or =1:500 in 4 (0.6%) dogs. These data indicate that feral dogs may be important in the epidemiology of N. caninum infection. 相似文献
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Sarashina T Uzuka Y Tanabe S Oku Y Watanabe Y Kurosawa N Nishimura M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):873-875
Fecal survey of Eimeria oocysts and parasite eggs was conducted for 219 fecal samples of free-ranging Grus japonensis in Kushiro district in Hokkaido in April 2003. Positive rate and mean oocysts (or eggs) per gram in positive samples were 26% (57/219) and 8.8 (0.2-136) in oocysts of Eimeria reichenowi, 18.3% (40/219) and 320 (100-1,000) in trematode eggs, 0.1% (2/219) and 0.2 (0.2-0.3) in eggs of Nematoda A, and 4.1% (9/219) and 0.8 (0.2-3.6) in eggs of Nematoda B, respectively. 相似文献
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Prevalence of cryptosporidiosis in dogs and human beings in San Bernardino County, California 总被引：2，自引：0，他引：2
A el-Ahraf J V Tacal M Sobih M Amin W Lawrence B W Wilcke 《Journal of the American Veterinary Medical Association》1991,198(4):631-634
Fecal specimens from 200 stray dogs impounded at the San Bernardino City and County animal shelters were screened for Cryptosporidium sp oocysts, using the auramine-rhodamine fluorescent staining procedure. The University of California, Los Angeles acid-fast stain was used for confirmation. Only 4 (2%) dogs were passing cryptosporidial oocysts. Likewise, stool specimens from 664 people were submitted to the San Bernardino County Department of Public Health Laboratory for routine parasitologic examination and were screened for Cryptosporidium sp. Cryptosporidial oocysts were detected in 20 human fecal specimens (3.01%). On the basis of these findings, it appears that the human and canine populations of San Bernardino County are at low risk for development of cryptosporidiosis at this time. 相似文献
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Sager H Moret CS Müller N Staubli D Esposito M Schares G Hässig M Stärk K Gottstein B 《Veterinary parasitology》2006,139(1-3):84-92
The protozoan parasite Neospora caninum is one of the most important abortifacient organisms in cattle worldwide. The dog is known to act as definitive host although its potential role as infection source for bovines still remains unelucidated. The aim of the present study was to compile initial epidemiological data on the prevalence and incidence of N. caninum in Swiss dogs acting as definitive hosts. Thus, 249 Swiss dogs were investigated coproscopically in monthly intervals over a period of 1 year. A total of 3289 fecal samples was tested by the flotation technique. Among these, 202 were shown to contain Sarcocystis sp. (6.1%), 149 Cystoisospora sp. (=Isospora sp.; 4.5%) and 25 Hammondia/Neospora-like oocysts (HNlO) (0.7%). All but one sample containing HNlO were from different dogs; one dog shed HNlO at two subsequent time points. Calculation of the yearly incidence for HNlO resulted in the surprisingly high value of 9.2%. Farm dogs exhibited a higher incidence for HNlO than urban family dogs. Thirteen out of the 25 HNlO-samples showed sporulation after 5 days incubation at room temperature. HNlO were further differentiated by species-specific PCR. However, all HNlO-samples were negative for N. caninum, Hammondia heydorni and Toxoplasma gondii. One reason may be the low oocyst density found in most fecal samples, which did not permit us to carry out PCR under optimal conditions. Three out of the 25 HNlO-cases contained enough oocysts to allow further enrichment and purification by the flotation technique. Subsequently, twenty to fifty sporulated HNlO-oocysts were orally administered to Meriones unguiculatus. All gerbils were seronegative for N. caninum at 5 weeks p.i. A N. caninum-seroprevalence of 7.8% was determined by ELISA upon 1132 serum samples collected from dogs randomly selected by veterinarians among their clinical patients. 相似文献
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Helminth and protozoan parasites in dogs and cats in Belgium 总被引：1，自引：0，他引：1
This study investigates the level of helminthic and protozoal infestation over the last 10 years in strays, well-cared-for dogs and cats. Determination of the prevalence of infections was based either on faecal examination or on worm counts at necropsy. Of 2324 faecal flotations (NaCl sp.gr. 1.20) of stray dogs, 34.2% had eggs or proglottids of one or more worm species consisting of Toxocara canis (17.4%), Toxascaris leonina (10.1%), Uncinaria stenocephala (11.4%), Trichuris vulpis (7.0%) and cestodes (2.1%). Isospora oocysts were observed in 5.2% of the dogs. The data on the distribution of the various worm species in the positive dogs indicate that T. canis eggs were by far the most common (50.9%). Necropsy data from 212 infected dogs indicate that 38.9% were infected with T. canis and 33.7% with T. leonina. The overall prevalence of worm infestation of 246 well-cared-for kennel dogs, based on worm egg counts by the McMaster technique, was 36.1%. Of 30 feline faecal samples examined by flotation, 83.3% were positive for parasites, including Toxocara cati (60%), Ancylostoma tubaeformae (36.6%), Taenia (Hydatigera) taeniaeformis (20%) and coccidia (30%). Toxocara cati was the most frequently found worm species at the necropsy of 25 cats (52%). Toxoplasma was not observed. 相似文献
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Ho SY Watanabe Y Lee YC Shih TH Tu WJ Ooi HK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(1):69-70
Intestinal helminth and protozoan infection in the quarantined dogs in Taiwan were examined using fecal examination between January to December, 2004. Of the 376 dogs imported from 11 countries, 63 (16.8%) were found to be infected with at least one species of intestinal parasite. The parasites detected were oocysts of Isospora canis and eggs of Toxocara canis, Trichuris vulpis and hookworms. Of the 63 infected dogs, 11 were found to have a mixed infection of two different species of parasites. This paper illustrates that parasites are transmitted from one country to another through the transport of animals. Moreover, there is also a possibility of parasitic infection among quarantined dogs as well as the zoonotic potential for quarantine officers during the quarantine period. 相似文献
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A cross-sectional study of Leishmania spp. infection in clinically healthy dogs with polymerase chain reaction and serology in Greece 总被引：3，自引：0，他引：3
Leontides LS Saridomichelakis MN Billinis C Kontos V Koutinas AF Galatos AD Mylonakis ME 《Veterinary parasitology》2002,103(1-2):19-27
The prevalence of gastrointestinal parasites in stray dogs, and dogs with owners was investigated by fecal examinations from 271 dogs employing sedimentation, simple flotation and centrifugation-flotation methods. The centrifugation-flotation method, when compared to simple flotation or sedimentation methods was generally more accurate in the diagnosis of all intestinal parasites, but statistical differences were detected only in relation to Giardia spp. and Cystoisospora spp. (synonym Isospora spp.). The following parasites, with their respective prevalence, were diagnosed in the fecal samples: Ancylostoma spp. (23.6%); Toxocara canis (5.5%); Trichuris vulpis (4.8%); Spirocerca lupi (1.9%); Dipylidium caninum (0.7%); Giardia spp. (12.2%); Hammondia heydorni (2.6%); Cystoisospora spp. (8.5%); and Sarcocystis spp. (2.2%). The prevalence of most parasites was similar for dogs of mixed-breed and for dogs of a defined-breed, except for Cystoisospora spp. and T. canis which showed a significantly higher prevalence in mixed-breed dogs. The prevalence of Ancylostoma spp. (17.1%) was significantly lower in stray dogs than in those with an owner (31.9%) and the prevalence of Giardia spp. and Cystoisospora spp. was higher in stray dogs (P < 0.05). No effect of season on the occurrence of the different parasite genera could be observed, except for Ancylostoma spp., for which an increase in the percentage of dogs shedding eggs was observed at the beginning of Summer with a peak occurrence during April and May (Autumn). The prevalence of Ancylostoma spp., T. canis, T. vulpis, Giardia spp. and Cystoisospora spp. was higher in adult males than in adult females, but significant differences between the two groups occurred only with Giardia spp. Young animals were found to more frequently shed Nematode eggs in feces than adult animals. 相似文献
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Training and racing constitute serious challenges for working sled dogs. Attainment of the highest levels of stamina and speed are possible only by completely healthy dogs. Infections with nematodes as whipworm Trichuris sp. or hookworms Uncinaria/Ancylostoma can significantly reduce the fitness of working dogs leading to anemia or even to death. In the middle of the racing season, between December 2009 and April 2010, 108 individual fecal samples were collected from 25 sled dog kennels situated in different regions of Poland. Saturated salt flotation was performed for helminth egg detection. The immunofluorescent assay MeriFluor Cryptosporidium/Giardia and nested PCRs on 18S rRNA (Cryptosporidium spp.) and TPI gene (Giardia spp.) were carried out for detection of intestinal protozoa. Overall prevalence of 6 species of intestinal parasites was 68% in sled dogs (73/108). In 51 samples the eggs of a single species of helminth were detected (47%), two nematode species were detected in 13%, three species of nematodes were found in two dogs. The most prevalent helminths were the hookworms Uncinaria/Ancylostoma-identified in 36% of kennels, and in 34% of sled dogs. Toxocara eggs were detected in 36% of kennels, in 17% of dogs. Trichuris sp. eggs were found in 20% of kennels (5/25), in 13% of dogs. Cysts/oocysts of intestinal protozoa were detected in 31% of sled dogs. The most prevalent was Giardia spp. infection-in 54% of kennels [13/24], in 28% of dogs. Cryptosporidium spp. infections were identified in 37.5% of kennels [9/24], in 13% of dogs. Two sequenced Giardia isolates presented 100% homology with G. intestinalis Assemblage C isolate (AY228641.1), specific for dogs. A range of factors was shown to affect the prevalence of intestinal parasites in sled dogs. The highest prevalence of parasites was found among dogs from large kennels (housing >3 dogs), in dogs less than 2 years old, and in kennels, where prophylactic treatment was carried out 1-4 times a year. The present study has demonstrated a high prevalence of intestinal parasites in working sled dogs in Poland, including the zoonotic human pathogens Toxocara or Cryptosporidium. 相似文献
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【目的】调查广东省内部分地区马匹寄生虫感染情况,探索区域内马匹寄生虫感染优势虫种、感染情况和耐药情况,为区域内马肠道寄生虫病的防治提供参考。【方法】采用饱和食盐溶液漂浮法和饱和蔗糖溶液漂浮法对广东省内7个城市24个马术俱乐部375份新鲜马粪样品进行处理,光学显微镜下观察虫卵/卵囊的形态结构特征并进行分类,并根据麦氏计数法计算每克粪便中虫卵/卵囊数（EPG）,统计感染率和感染强度。对EPG＞500的阳性样本进行粪便虫卵减少量计数试验（FECRT）,以评估各马术俱乐部流行寄生虫耐药情况。提取5份＜1岁幼驹的粪便样本DNA,利用巢式PCR检测样品是否存在隐孢子虫感染。【结果】375份样品中有120份样品呈寄生虫虫卵/卵囊阳性,总感染率为32.00%。共检出4种肠道寄生虫,分别为圆线虫、马副蛔虫、类圆线虫和马蛲虫,总感染率分别为30.40%、3.70%、0.80%和0.30%。1~4岁马匹感染率显著高于其他年龄段（P＜0.05）,但不同性别马匹寄生虫感染情况无显著差异（P＞0.05）。受调查马匹感染强度以低排卵（EPG＜200）为主,占马匹总数83.47%。FECRT结果显示,样本所在马术俱乐部常用驱虫药（主要成分为伊维菌素、阿苯达唑）有效,未见耐药现象。5份＜1岁幼驹的粪便样本隐孢子虫巢式PCR检测均为阴性。【结论】广东省部分地区马术俱乐部道寄生虫防控情况总体良好,区域内各马术俱乐部寄生虫防控效果存在差异,建议区域内各马术俱乐部加强马肠道寄生虫监测工作,依据本场监测情况合理用药,避免场内流行寄生虫对所用驱虫药物产生耐药性。 相似文献