Familial Congenital Methemoglobinemia in Pomeranian Dogs Caused by a Missense Variant in the NADH‐Cytochrome B5 Reductase Gene          下载免费PDF全文

H. Shino  Y. Otsuka‐Yamasaki  T. Sato  K. Ooi  O. Inanami  R. Sato  M. Yamasaki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):165-171

Background

In veterinary medicine, congenital methemoglobinemia associated with nicotinamide adenine dinucleotide (NADH)‐cytochrome b5 reductase (b5R) deficiency is rare. It has been reported in several breeds of dogs, but little information is available about its etiology.

Objectives

To analyze the NADH‐cytochrome b5 reductase gene, CYB5R3, in a Pomeranian dog family with methemoglobinemia suspected to be caused by congenital b5R deficiency.

Animals

Three Pomeranian dogs from a family with methemoglobinemia were analyzed. Five healthy beagles and 5 nonrelated Pomeranian dogs without methemoglobinemia were used as controls.

Methods

Methemoglobin concentration, b5R activity, and reduced glutathione (GSH) concentration were measured, and a turbidity index was used to evaluate Heinz body formation. The CYB5R3 genes of the affected dog and healthy dogs were analyzed by direct sequencing.

Results

Methemoglobin concentrations in erythrocytes of the affected dogs were remarkably higher than those of the control dogs. The b5R activity of the affected dogs was notably lower than that of the control dogs. DNA sequencing indicated that this Pomeranian family carried a CYB5R3 gene missense variant (ATC→CTC at codon 194) that resulted in the replacement of isoleucine (Ile) by leucine (Leu).

Conclusions and Clinical Importance

This dog family had familial congenital methemoglobinemia caused by b5R deficiency, which resulted from a nonsynonymous variant in the CYB5R3 gene. This variation (c.580A>C) led to an amino acid substitution (p.Ile194Leu), and Ile194 was located in the proximal region of the NADH‐binding motif. Our data suggested that this variant in the canine CYB5R3 gene would affect function of the b5R in erythrocytes.  相似文献   

Competence of Aedes albopictus and Culex quinquefasciatus as vector of Dirofilaria immitis after blood meal with different microfilarial density     

Lai CH  Tung KC  Ooi HK  Wang JS 《Veterinary parasitology》2000,90(3):231-237

Aedes albopictus and Culex quinquefasciatus were fed canine blood with different microfilarial density of Dirofilaria immitis ranging from 2500 to 25,000 mff/ml. Larval development in these two mosquito species did not differ significantly. Although C. quinquefasciatus ingested more microfilariae, the number of larvae which developed in A. albopictus was invariably greater than in C. quinquefasciatus. Mortality of the engorged A. albopictus was significantly greater than that of C. quinquefasciatus, and higher microfilarial density raised the mortality in both species. The vector efficiency index of A. albopictus was greater than C. quinquefasciatus at all microfilarial densities, but its survival time was much reduced. Thus, dogs with low microfilarial density are implicated as the main source for the transmission of D. immitis from dogs to mosquitoes.  相似文献   

Mechanochemical properties of ordinary and dark muscle myosins from seawater fish     

Tsuyoshi Okagaki  Lin Yang  Atsushi Ooi 《Fisheries Science》2007,73(1):184-188

Myosin was isolated from two types of muscle, ordinary and dark muscles, of three species of fish living in sea water. The compositions of light chains were visualized by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the mechanochemical activity was examined by in vitro motility and ATPase assays. Ordinary muscle myosin of either species had three species of light chain, whereas dark muscle myosin had another two species of light chain judged by SDS-PAGE. Sliding velocity of ordinary muscle myosin was in the range of 4.92–6.89 μm/S, whereas that of dark muscle myosin was in the range of 3.07–4.25 μm/s. Therefore, ordinary muscle myosin showed 1.26–1.95 times higher sliding velocity than dark muscle myosin in either species. The ratios of V_max of actin-activated Mg²⁺-ATPase activity of ordinary to dark muscle myosins were correlated quite well to the ratios of sliding velocity. Activity of ordinary muscle myosin was comparable to that of mammalian fast muscle myosin, but that of dark muscle myosin was twice of that of mammalian slow muscle myosin. These results may reflect the essential role of fish dark muscle myosin always used in slow cruising.  相似文献   

Thermal stability of carp G-actin monitored by loss of polymerization activity using an extrinsic fluorescent probe     

Atsushi Ooi  Fumiaki Yano  Tsuyoshi Okagaki 《Fisheries Science》2008,74(1):193-199

ABSTRACT:   The thermal stability of carp G-actin was investigated by monitoring loss of actin polymerization ability. To determine the amount of native actin remaining after heat treatment, actin was labeled with a fluorescence reagent, N-(1-pyrene)iodoacetamide. The loss of polymerization ability of carp actin during heat treatment, at between 45 and 55°C, occurred faster than that of chicken actin. The inactivation rate was influenced by concentrations of ATP and Ca²⁺ in solution. With the increase of Ca²⁺ concentration, the inactivation of carp actin was markedly suppressed. Furthermore, the activation energy of the inactivation of carp actin obtained from an Arrhenius plot was similar to that of chicken actin. These results indicated that the thermal instability of carp G-actin was due to the low affinites of ATP and Ca²⁺ for carp actin described in a previous report.  相似文献   

Some evidence for genetically controlled variation in photosynthetic rate of oil palm seedlings     

R. H. V. Corley  J. J. Hardon  S. C. Ooi 《Euphytica》1973,22(1):48-55

Summary The rate of uptake of ¹⁴CO₂ by oil palm seedlings in relation to light intensity was studied in a complete diallel cross. Analysis showed heritable differences in both light saturated and light limited photosynthesis, and in leaf chlorophyll content. The potential importance of this in a breeding programme is discussed.  相似文献   

Chemistry. Heat and light switch a chiral catalyst and its products     

Ooi T 《Science (New York, N.Y.)》2011,331(6023):1395-1396

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Critical roles for Rac1 and Rac2 GTPases in B cell development and signaling     

Walmsley MJ  Ooi SK  Reynolds LF  Smith SH  Ruf S  Mathiot A  Vanes L  Williams DA  Cancro MP  Tybulewicz VL 《Science (New York, N.Y.)》2003,302(5644):459-462

The Rac1 guanosine triphosphatase (GTPase) has been implicated in multiple cellular functions, including actin dynamics, proliferation, apoptosis, adhesion, and migration resulting from signaling by multiple receptors, including the B cell antigen receptor (BCR). We used conditional gene targeting to generate mice with specific Rac1 deficiency in the B cell lineage. In the absence of both Rac1 and the highly related Rac2, B cell development was almost completely blocked. Both GTPases were required to transduce BCR signals leading to proliferation, survival and up-regulation of BAFF-R, a receptor for BAFF, a key survival molecule required for B cell development and maintenance.  相似文献   

A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A     

Cruz-Migoni A  Hautbergue GM  Artymiuk PJ  Baker PJ  Bokori-Brown M  Chang CT  Dickman MJ  Essex-Lopresti A  Harding SV  Mahadi NM  Marshall LE  Mobbs GW  Mohamed R  Nathan S  Ngugi SA  Ong C  Ooi WF  Partridge LJ  Phillips HL  Raih MF  Ruzheinikov S  Sarkar-Tyson M  Sedelnikova SE  Smither SJ  Tan P  Titball RW  Wilson SA  Rice DW 《Science (New York, N.Y.)》2011,334(6057):821-824

The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.  相似文献   

Infectivity in rodents and cold resistance of Trichinella spiralis isolated from pig and polar bear, and T. pseudospiralis     

H K Ooi  M Kamiya  M Ohbayashi  M Nakazawa 《The Japanese journal of veterinary research》1986,34(2):105-110

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The effect of radiation on the viability and migratory ability of second-stage larvae of Toxocara canis in mice     

M Kamiya  H K Ooi  T Nomura 《Veterinary parasitology》1987,24(1-2):87-92

Larval counts were made on mice 2 days after oral inoculation with X-ray (0-320 Krad) or of gamma ray (0-6 Mrad) irradiated eggs containing second-stage Toxocara canis larvae. The majority of the larvae irradiated with 0-40 Krad were recovered from the liver and lungs, while most of the larvae irradiated with 80 or 160 Krad remained in the digestive tract, mainly in the stomach and the proximal half of the small intestine. Only a small number of the 320 Krad irradiated larvae was recovered from the mice. No significant difference was observed in the viability of irradiated larvae incubated in vitro up to 13 days after irradiation. However, a substantial percentage of the 160 and 320 Krad-irradiated larvae hatched during that period. Very few larvae were recovered from the digestive tract of mice inoculated with eggs irradiated with 0.5 Mrad, and only one and four larvae were recovered from the liver and lungs of a mouse. No visceral larval migration was observed in mice inoculated with 1 Mrad-irradiated eggs. The minimum lethal radiation dose for second-stage T. canis larvae in eggs is proposed to be 1 Mrad.  相似文献