共查询到20条相似文献，搜索用时 343 毫秒
1.
建立高效的水产品硝基呋喃类代谢物检测分析方法,是保障我国水产品质量安全的一种有效风险监测技术手段。超高效液相色谱-串联质谱法(UPLC-MS/MS)具有分析时间短、检出限低、灵敏度高等优点,可满足水产品硝基呋喃类代谢物的定性和定量分析。介绍了硝基呋喃类药物种类、特点及其危害,对UPLC-MS/MS技术在水产品硝基呋喃类代谢物的检测应用做了简要综述。 相似文献
2.
3.
阿维菌素类药物是一种常用的杀虫剂,现有报道的食品和环境基质样品中阿维菌素残留分析最为常用的检测仪器为液相色谱-串联质谱。基于此,已建立了一系列的不同样基质中多种阿维菌素类药物残留量的检测方法,本研究除了关注常见的多种阿维菌素残留量的测定方法,也关注了食品和环境样品中单种阿维菌素化合物的专一性检测方法。本研究从阿维菌素在食品和环境领域的应用以及赋存状态、前处理方式（提取和净化方法）、检测方法（液相色谱法、酶联免疫法和液相色谱-串联质谱法）及定量方式和基质干扰等方面,综述了2010—2020年不同检测技术在食品和环境样品基质中阿维菌素类化合物残留分析中的研究与应用进展。最后,综述了多残留量检测方法的局限性,未来的关注点以及对阿维菌素残留分析方法的发展趋势。本研究可为开展烟草中阿维菌素类化合物残留分析技术研究提供参考。 相似文献
4.
建立应用超高效液相色谱串联质谱法同时检测马铃薯中烯酰吗啉、氟吡菌胺及其代谢物BAM的残留分析方法,并对其在马铃薯中的储藏稳定性进行了研究。样品采用QuEChERS方法处理,经乙腈提取,PSA和C18净化,质谱多反应监测模式扫描,外标法定量。试验结果表明:在0.01、0.10、0.50 mg/kg三个浓度的添加水平下,烯酰吗啉、氟吡菌胺及其代谢物BAM在马铃薯样品中平均添加回收率为90%~96%,相对标准偏差为0.6%~5.5%。该方法简便高效,可用于烯酰吗啉、氟吡菌胺及其代谢物BAM在马铃薯中的快速检测。在低于-18℃条件下储藏6个月的试验期间,烯酰吗啉、氟吡菌胺及其代谢物BAM的平均降解率为0~19%,表明它们在马铃薯基质中均比较稳定。 相似文献
5.
6.
7.
高效液相色谱串联质谱法检测菠菜中三乙磷酸铝残留量 总被引：1，自引：0，他引：1
建立了高效液相色谱串联质谱检测菠菜中三乙磷酸铝残留量的方法,回收率达到103%,相对标准偏差为6%,定量下限0.27 mg/kg,0.2~10 mg/L的相关系数大于0.99。该方法操作简单,重现性和准确度高,缩短了菠菜中三乙磷酸铝检测的时间。 相似文献
8.
《农产品加工．学刊》2014,(1)
食品农药残留问题成为人们普遍关注的社会热点问题,生姜中农药检测方法主要有气相色谱法、气相色谱-质谱法、液相色谱-电喷雾串联质谱法和超高效液相色谱-串联质谱法等。对这些检测方法进行系统总结,以期为生姜农药进一步检测提供技术选择参考。 相似文献
9.
建立了婴幼儿配方食品中胆碱和左旋肉碱含量的高效液相色谱-串联质谱检测方法。样品通过水提取后,用冰乙酸沉淀蛋白质,再用液相色谱-串联质谱法分离测定。胆碱和左旋肉碱在相应含量范围内线性良好,相关系数大于0.995。当胆碱加标200～800 mg/kg时,回收率为80.3%～107.9%,相对标准偏差3.6%～7.5%;左旋肉碱加标20～80 mg/kg时,回收率为75.4%～95.7%,相对标准偏差6.0%～8.2%,方法检测限为1.0 mg/kg。该方法简便、快速、准确,可用于婴幼儿配方食品中胆碱和左旋肉碱的定性、定量分析。 相似文献
10.
中国鲜活水产品市场增长迅猛,但产销地域差别明显,长距离运输需求显著。镇静剂能够抑制机体的新陈代谢,降低鲜活水产品应激反应,其被应用于水产品运输、繁育和手术等环节,以提高水产品的成活率。但是镇静剂在水产品中广泛使用而可能导致的残留问题已经引起了国内外研究人员的关注。本文对水产品中镇静剂残留检测技术研究进展进行了概述,以期为后续研究提供参考。对水产品中镇静剂的类型及使用规定进行了介绍,分析并总结了近10年来水产品中镇静剂类药物的检测研究进展。论述了水产品中镇静剂残留检测样品前处理方法、高效液相色谱检测方法、高效液相色谱串联质谱法和气相色谱串联质谱法进展,归纳了相关方法的优缺点,并对其发展做出展望。在目前研究的基础上,未来应在新型简单、高效样品前处理方法方面开展更深入的研究,为水产品质量安全监管提供技术支撑。 相似文献
11.
How to remove the background , to eliminate the misdetection caused by vehicle changes road or it covers the detection window of the road detected partly, is a big problem to be handled in vehicle detection system.This paper presents an improved frame-difference method based on Video,and describes how to detect and count vehicles in detecting zone dynamically.With this method, we can count the vehicles passing by accurately, collect the traffic flow of each roadway reliably.We provide real-time traffic parameters for the ITS. 相似文献
12.
马铃薯A病毒液相芯片快速检测方法的建立 总被引：2，自引：2，他引：0
旨在建立可检测马铃薯A病毒(Potato virus A,PVA)的液相芯片快速检测技术,用Primer Premier 5.0软件对GenBank中PVA核苷酸序列进行分析,设计其特异性探针并用生物素标记。探针偶联荧光编码微球后与PVA的PCR产物杂交反应,用液相芯片检测仪检测荧光信号。结果显示该法具有较好的特异性,不与侵染马铃薯的番茄黑环病毒(Tomato black ring virus,TBRV)、马铃薯纺锤块茎类病毒(Potato spindle tuber viroid,PSTVd)、马铃薯Y病毒(Potato virus Y,PVY)及番茄斑萎病毒(Tomato spotted wilt virus,TSWV)反应;检测灵敏度约为10 pg/μL总RNA。该方法可用于PVA的快速检测。 相似文献
13.
An image edge detection algorithm in fuzzy domain is proposed, which combines adaptive fuzzy enhancement and multi direction fuzzy morphology to detect the edges of fuzzy image. The adaptive fuzzy enhancement method enhances the fuzzy image within blocks with sliding windows to avoid losses of the real edges resulting from enhancing with single threshold for the whole image and lead strong adaptive ability to image region variance. The multi direction fuzzy mathematical morphology operates on the enhanced fuzzy image with structure elements of multiple different directions to extract the real edges with directionality and restrain non directional noise. Experiments show the algorithm can detect fuzzy image edge effectively with strong antinoise ability. 相似文献
14.
Intrusion detection system is an important component of the computer and information security framework. Its main goal is to differentiate between normal activities of the system and behaviors that can be classified as suspicious or intrusive, and its main challenge is to efficiently detect intrusion detection behaviors for reducing false positive rate and false negative rate. In view of the disadvantages of the existing intrusion detection methods, fuzzy c-means(FCM) clustering method is used to analyze intrusion detection data in order to detect anomaly network behavior patterns. Experimental results on the CUP99 data set data show that this method can not only feasible but also improve the accuracy and efficiency. 相似文献
15.
Building management control systems (BMCS) are widely employed in modern buildings. The huge amount of data available on central stations and outstations provide rich information for fault diagnosis of HVAC systems. An online fault diagnosis method for variable air volume air handling units was presented using self-tuning HVAC component models. The model parameters are tuned online by using a genetic algorithm (GA) which minimizes the error between measured and estimated performance data, so high modeling accuracy is assured. If the error between measured and estimated performance data exceeds preset thresholds, it means the occurrence of faults or abnormalities in the air handling unit system. The statistical method of selecting thresholds also is presented. The fault detection method was tested and validated using data collected from real HVAC systems. The results of validation show that the fault detection method can be integrated in BMCS systems to detect faults in air handling unit systems efficiently. 相似文献
16.
The conventional mechanical transmissions can be retro fitted to carry out the gearshift automatically.Their shifts must be detected by the sensors and controlled by the actuators.The method in detecting the shift with non contacted photoelectricity elements is proposed.For 5 speed gearboxes,two photoelectricity elements are used to detect three gear selecting positions,the other are used to two detect three gear shifting positions.The appropriate arrangement of switch inputs can make the nine shifts of a 5 speed gearbox be detected by four photoelectricity elements.Therefore the necessary switch numbers decrease,the necessary hardware is more simple and the control software is easy.The principle of shift detection and the program flow chart of shift control are given. 相似文献
17.
Collecting real-time traffic flow information is a foundational function in intelligent transportation systems. Video based traffic monitoring system has advantage of installation and maintenance, so it iswidely used for traffic monitoring on main road and crossroad. Shadows result from roadside building or trees are one of the factors that arise errors in video based vehicle detection. An improved method based on edge information is proposed for vehicle detection and shadow rejection. Finally vehicle counting is achieved based on the edge detection method. 相似文献
18.
应用纳米磁珠荧光PCR检测棉花曲叶病毒 总被引：1，自引：1，他引：0
为了提供快速灵敏检测棉花曲叶病毒(Cotton leaf curl virus, CLCuV)的技术以防止其传播扩散,本研究根据CLCuV外壳蛋白基因（Coat protein, CP）保守序列设计了引物和TaqMan探针,并结合纳米磁珠(Magnetic nanoparticles, MNPs)建立了该病毒的MNP实时荧光PCR (Real-time PCR)检测方法。该方法检测阈值约为525 fg·μL-1 DNA。通过对CLCuV、非洲木薯花叶病毒、烟草线条病毒、黄瓜花叶病毒及番茄斑萎病毒的检测表明,该方法具有良好的特异性;同时该方法无需任何PCR后处理,交叉污染风险小。本方法可用于CLCuV的快速检测。 相似文献
19.
Some thoroughly research on the basic method for load state detection in MIN is introduced. Load sources of SCP and SSP in MIN are analyzed in detail after investigating the relationship between inter resource and load state in the system. And load detection parameters and model are presented. Moreover, a novel strategy to detect system load state based on fusion of multi detection data is presented. By some test, the theory and method are proved most feasible. Our research show that load state of MIN can be recognized by detecting utility of internal resource utilizing of single node in MIN, and multi redundant detecting data fusion is effective approach to improve the reliability and trust degree of the result. 相似文献
20.
为了快速进行甘薯病毒田间检测和脱毒苗诊断,该研究建立了一个能够同时检测SPCSV、SPLV和SPFMV这3种病毒的多重RT-PCR检测方法。根据GenBank公示的甘薯病毒基因序列来设计3种甘薯病毒的引物,分别对模板浓度、退火温度、Mg2+浓度和循环次数进行优化,同时对模板浓度进行10倍梯度稀释,检测多重RT-PCR灵敏度。结果显示,模板浓度为19 ng/μL、退火温度为52℃、Mg2+浓度为0 mmol/L、循环次数为40次时,可以同时扩增出大小为276 bp、570 bp、425 bp三种病毒的基因片段,多重RT-PCR检测灵敏度为10-4,单一RT-PCR检测灵敏度为10-3,多重RT-PCR检测灵敏度是单一RT-PCR的10倍。该研究使用优化的多重RT-PCR检测技术可获得稳定、灵敏、准确、高效地和同时地检测田间复合侵染的3种甘薯病毒。 相似文献