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1.
人绒毛膜促性腺激素对母驴促排卵效果的研究 《畜牧与饲料科学》2021,42(3):59-65
[目的]研究人绒毛膜促性腺激素（human chorionic gonadotropin,hCG）对母驴卵泡发育、排卵率、受胎率以及血清生殖激素水平的影响。[方法]选择优势卵泡直径在30~35 mm以及大于35 mm的母驴各30头,不同优势卵泡直径的母驴群体分别设置1个500 IU/头hCG处理组（n=10）、1个1 000 IU/头hCG处理组（n=10）、1个不接受hCG处理的对照组（n=10）。采用肌肉注射方法对各组母驴进行hCG处理。每隔24 h进行1次B超检查,观察各组母驴卵泡发育情况,测量卵泡直径;记录各组发生排卵的母驴数量,计算排卵率。对各组母驴进行人工输精,输精后第18天进行孕检,记录各组受胎母驴头数,计算各组受胎率。于hCG处理后0、24、48、72 h分别测定各组母驴血清中雌二醇（estradiol,E2）和孕酮（progesterone,PROG）水平。[结果]2个群体母驴的卵泡直径随hCG注射剂量的增加而增大;优势卵泡直径大于35 mm的母驴群体中,肌肉注射hCG的2个组在处理后24 h内均出现排卵,而对照组母驴没有排卵;优势卵泡直径不同的2个母驴群体,在hCG处理48 h后排卵母驴数和排卵率与对照组相比均有所提高,其中,hCG处理后72 h,优势卵泡直径大于35 mm的母驴群体中,1 000 IU/头 hCG处理组的排卵率达到100%。2个母驴群体中,接受hCG处理的母驴,受胎率均高于对照,并且随hCG剂量的增加,受胎率有所提高;优势卵泡直径大于35 mm的母驴群体中,1 000 IU/头 hCG处理组的受胎率达到50%。2个母驴群体中,1 000 IU/头处理组在hCG处理后24 h的血清E2浓度均较0 h时有较大幅度的提升,在0~72 h内血清PROG浓度的总体提升幅度较大。[结论]hCG处理可提高母驴的排卵率、受胎率以及血清中E2和PROG水平,1 000 IU/头剂量的效果更好。 相似文献
2.
为了研究新疆地区在寒冷条件下西门塔尔牛的同期发情效果与如何提高受胎率的措施方面展开试验。采用氯前列烯醇(PGF2a)一次法和孕马血清促性腺激素(PMSG)一次法进行试验,剂量分别是PG为0.8 mg/头,PMSG为500 IU/头;共分为2组,A组89头牛;B组为121头牛。结果表明,A组:PGF2a一次法的同期发情效果为82.2%,受胎率为67.6%;PMSG一次法的同期发情效果为72.7%,受胎率为53.1%。B组:PGF2a一次法的同期发情效果为80.0%,受胎率为66.7%;PMSG一次法的同期发情效果为73.8%,受胎率为51.1%。通过本试验为新疆地区在寒冷条件下开展类似的工作提供借鉴。 相似文献
3.
不同生殖激素对乏情母猪的应用效果试验 总被引：1，自引：0，他引：1
通过目前常用的3种不同生殖激素处理乏情母猪的效果比较，证实氯前列烯醇（PGF）＋孕马血清促性胰激素（PMSG）组效果最佳，2周内发情率为93．3％，受胎率为85．7％，分娩率为78．5％，平均窝产仔数9．7头，窝产活仔数9．45头。 相似文献
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为了筛选出合理的肉牛同期发情处理方案,试验选用健康能繁母牛90头,随机分为5组,采用黄体酮阴道栓(P)、孕马血清促性腺素(PMSG)、注射用促黄体素释放激素A_3(A_3)、氯前列醇钠注射液(PGF)4种药物,按5种不同方案(P/PGF/PMSG、P/PGF/A_3、PGF/A_3/PGF/A_3、PGF/PGF/PMSG、PGF/PMSG)进行同期发情处理,母牛发情后用细管冷冻精液输精,测定其同期发情率、情期受胎率和处理牛受胎率。结果表明:方案一(P/PGF/PMSG)情期受胎率最高,方案四(PGF/PGF/PMSG)处理牛受胎率最高,方案五(PGF/PMSG)情期受胎率和处理牛受胎率均最低。说明方案一到方案四均可用于肉牛的同期发情处理。 相似文献
6.
将舍饲条件下有发情表现的土杂母羊15只集中饲养并用海绵阴道栓(含黄体酮50 mg)处理15 d。将处理后的羊只分为3组,A组撤拴后不做任何处理,B组撤栓后注射PGF2α0.025 mg/只,C组撤拴后注射PGF2α0.05 mg/只,然后观察羊只的发情表现。结果A组在24-48 h内全部发情,同期发情率达100％,B组在48-96 h内有4只发情,同期发情率80％,C组有3只发情,同期发情率为60％。从取栓到发情的时间A、B和C组分别为38.4±7.7 h、87.2±20.1 h和81.2±28.9 h。 相似文献
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摘要：本文应用FSH (促卵泡生成素)等药物对后备母猪和乏情母猪进行疗效试验。结果发现,后备母猪的FSH组和PMSG (孕马血清)组发情率分别为65.39%和66.67%,两组相比无显著性差异(P>0.05);配种受胎率分别为82.35%和78.57%,两组相比差异显著(P<0.05);窝均产仔数分别为10.94头和9.14头,两组相比差异显著(P<0.05);对照组母猪的发情率、配种受胎率和窝均产仔数均不理想。对断奶30 d不发情的乏情母猪,FSH组和PMSG组发情率分别为85.71%和86.96%,两组相比无显著性差异(P>0.05);配种率受胎分别为86.67%和80%,两组相比差异显著(P<0.05);窝均产仔数分别为11.46头和10.06头,两组相比差异显著(P<0.05);对照组母猪的发情率、配种受胎率和窝均产仔数均不理想。表明FSH组和PMSG组对后备母猪、乏情母猪疗效显著,且FSH组优于PMSG组。 相似文献
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2005年8月初，对西藏藏北54头3～12岁受体母牦牛实施了同期发情试验，外源激素分别采用促排卵素LHRHA3结合孕马血清PMSG处理法和氯前列腺素（PGF2α）两次注射处理法。结果表明：LHRH—A3＋PMSG法处理6-9岁和10～12岁牦牛时发情率（可移植率）分别为93．3％（40％）和60％（20％），二次PGF2口法处理3～5岁、6～9岁、10～12岁牦牛时发情率（可移植率）分别为61．1％（5．5％）、70％（40％）和50％（16．7％）。结论：LHRH—A3＋PMS（；法和二次PGF3α法都是成功的同期处理方法，受体在6～9岁时同期效果最好。 相似文献
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为更好地开展肉牛杂交改良工作,研究不同同期发情处理方法对肉牛同期发情率和受胎率的影响,探索适宜推广的同期发情处理方法,本试验采用3种不同激素药物组合,组合Ⅰ（PMSG+FSH）、组合Ⅱ（PMSG+FSH+PG）、组合Ⅲ（PMSG+PG）,分别进行1次和2次注射,配合使用PMSG（促性腺激素）,通过FSH（促卵泡素）、肌肉注射前列腺素（PG）溶解黄体、FSH（促卵泡素）和前列腺素（PG）联合使用3种方法进行母牛同期发情处理对比试验。结果显示,采用3种不同激素药物组合,进行同期发情处理,同期发情率2次注射优于1次注射,受胎率组合Ⅱ>组合Ⅰ>组合Ⅲ,组合Ⅲ（PMSG+PG）2次注射效果最佳。研究表明,配合使用促性腺激素,可以增强发情同期化和提高发情,更好地促使卵泡成熟和排卵,提高其受胎率。 相似文献
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将５１头经产母猪随机分成两组，试验组３７头，于断奶后１２小时内注射猪宝灵６００ＩＵ，对照组不作处理，以观察同期发情率，情期受胎率和产仔质量。用猪宝灵分别处理４８头乏情母猪和１２头初情青年母猪，以观察其调节体内激素代谢水平，治疗某些繁殖疾病。研究结果表明：同期发情试验，受试组与对照组之间的同期发情率差异显著（Ｐ＜０．０１）。处理乏情母猪发情率８７．５％（４２／４８）情期受胎率８３．３％（３５／４２），初期青年母猪诱导发情率８３．３％（１０／１２），情期受胎率８０％（８／１０）。确立了猪宝灵处理不同类型母猪，对初情青年母猪有诱导性早熟作用，提高了经产母猪同期发情水平，同时又能有效地治疗乏情母猪，以达到最佳排卵率和较理想受胎率。因此猪宝灵的应用对提高母猪繁殖力，实现工厂化、集约化批次生产商品猪起了积极作用，产生了良好的经济效益，促进了养猪业的发展 相似文献
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Tsutomu TSUKADA Kunitada SATO Masaharu MORIYOSHI Masanori KOYAGO Yutaka SAWAMUKAI 《Journal of Equine Science》2008,19(2):35-38
A total of 88 thoroughbred mares were diagnosed with clinical ovarian quiescence and
subjected to four treatment regimens. Using PMSG, hCG or combinations of both. A high dose
combination of 5,000IU PMSG with 5,000IU hCG showed significantly higher rates of marked
estrus and ovulation induction (P<0.01) as well as conception rates (P<0.05). In the
present study, the administration of a high-dose combination of PMSG with hCG was shown to
be an effective treatment of ovarian quiescence in light mares. 相似文献
12.
Two experiments were conducted to examine the effect of prostaglandin F2 alpha (PGF2 alpha) and pregnant mares' serum gonadotropin (PMSG) on the reproductive performance (fertility, prolificacy and fecundity) of ewes previously treated with fluorogestone acetate (FGA) and PMSG. In the first experiment, 29 ewes were synchronized for estrus with FGA and PMSG but not bred at the postsynchronization estrus. On day 10 of the first post-synchronization estrous cycle, they were injected IM with 15 mg PGF2 alpha and 500 IU PMSG and exposed to experienced, fertile, raddled rams. Twenty-four of the 29 ewes (83%) had viable fetuses 9 weeks after the PGF2 alpha-PMSG treatment. In the second experiment, 64 ewes were treated with FGA-PMSG, and 33 were exposed to fertile, raddled rams at the synchronized estrus. A second group of 31 ewes was not bred at the synchronized estrus, but on day 12 of the postsynchronized estrous cycle, they were injected IM with 15 mg PGF2 alpha and 500 IU PMSG and exposed to fertile, raddled rams. Sixty-six percent of the 33 ewes lambed in the FGA-PMSG-treated group and 60% of the 31 ewes lambed in the PGF2 alpha-PMSG-treated group. Differences in reproductive performance between these two treatment groups were not statistically significant. The results suggest that the PGF2 alpha-PMSG treatment combination does not adversely affect reproductive performance of ewes. 相似文献
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同期发情处理方法对受体牛胚胎移植效果影响的研究 总被引：4，自引：0，他引：4
试验设计3个组．分别采用CIDR＋PGF2α法、PGF2α一次注射法和PGF2α二次注射法3种不同的处理方法对120头西门塔尔杂交受体牛进行同期发情处理。结果表明：CIDR＋PGF2α法同期发情处理的受体牛黄体不合格率达47．4％．显著高于其他两组（P〈0．05）;胚胎移植产犊率为45．0％,分别比PGF2α一次注射法和PGF2α二次注射法低10．2和13．1个百分点。PGF2α二次注射法同期发情处理后的受体牛黄体不合格率仅20．5％,低于PGF2α一次注射法（P〉0．05）,显著低于CIDR＋PGF2α法（P〈0．05）;胚胎移植产犊率达58．1％,均高于其他两组。本试验初步显示：采用PGF2α二次注射法进行同期发情处理受体牛效果好,成本较低,操作方便,产犊率也较高。 相似文献
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H Kamomae Y Kaneda I Domeki T Nakahara 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(3):467-473
Ovarian quiescent cattle bearing follicle with palpable size were treated with single intramuscular injection of 750-6,000 IU of human chorionic gonadotrophin (hCG) in 13 cases and 1,000-2,000 IU of pregnant mare serum gonadotrophin (PMSG) in 5 cases. Changes of blood luteinizing hormone (LH) level, estrus and ovulation after the treatments were examined. After the hCG treatment LH level became slightly high from 0.2-0.6 ng/ml of pre-treatment to 0.3-1.9 ng/ml of post-treatment and maintained the level up to ovulation without the ovulatory LH surge. Ovulation was induced about 36 hr after the treatment in 12 cases. The ovulations were all silent ovulations. After the PMSG treatment LH level became slightly high from 0.6 ng/ml of pre-treatment to 1.3 ng/ml of post-treatment and the level lasted until the ovulatory LH surge. The ovulatory LH surge occurred about 39 hr after the PMSG treatment in 4 cases with a peak of about 32 ng/ml. Ovulation was induced about 74 hr after the treatment in all 5 cases. Four cases showed estrus but one in which the LH surge could not be confirmed did silent estrus preceding the induced ovulations. It was demonstrated that hCG induced ovulation without the LH surge but PMSG induced the ovulatory LH surge and the subsequent ovulation in ovarian quiescent cattle. 相似文献
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Effect of human chorionic gonadotropin on preovulatory luteinizing hormone surge and ovarian hormone secretion in gilts 总被引：1，自引：0，他引：1
The influence of varying doses of human chorionic gonadotropin (hCG) on the preovulatory luteinizing hormone (LH) surge, estradiol-17 beta (E2) and progesterone (P4) was studied in synchronized gilts. Altrenogest (AT) was fed (15 mg X head-1 X d-1) to 24 cyclic gilts for 14 d. Pregnant mares serum gonadotropin (PMSG; 750 IU) was given im on the last day of AT feeding. The gilts were then assigned to one of four groups (n = 6): saline (I), 500 IU hCG (II), 1,000 IU hCG (III) and 1,500 IU hCG (IV). Human chorionic gonadotropin or saline was injected im 72 h after PMSG. No differences in ovulation rate or time from last feeding of AT to occurrence of estrus were observed. All gilts in Groups I and II expressed a preovulatory LH surge compared with only four of six and three of six in Groups III and IV, respectively. All groups treated with hCG showed a rapid drop (P less than .01) in plasma levels of E2 11, 17, 23 h after hCG injection when compared with the control group (35 h). The hCG-treated gilts exhibited elevated P4 concentrations 12 h earlier than the control group (3.1 +/- .5, 3.4 +/- .72, 3.1 +/- .10 ng/ml in groups II, III and IV at 60 h post-hCG vs .9 +/- .08 ng/ml in group I; P less than .05). These studies demonstrate that injections of ovulatory doses of hCG (500 to 1,500 IU) had three distinct effects on events concomitant with occurrence of estrus in gilts: decreased secretion of E2 immediately after hCG administration, failure to observe a preovulatory LH surge in some treated animals and earlier production of P4 by newly developed corpora lutea. 相似文献
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不同处理方法对水牛同期发情效果的观察 总被引：1，自引：0，他引：1
[目的]为筛选一套高效且适宜于生产推广的水牛同期发情处理方案。[方法]借助便携式兽用B超观察,用PGc一次注射法、PMSG＋PGc法和GnRH＋PGc＋GnRH法对水牛进行同期发情处理。[结果]发情率和排卵率分别为58.33%、84.21%、83.90%和54.17%、50.00%、75.42%,平均发情率为77.94%,平均排卵率为65.69%,GnRH法处理水牛同期发情获得较高的排卵率。黄体影响同期发情处理效果,对PGc、PMSG影响较大,对GnRH处理影响较小。[结论]GnRH法处理结束48h内有72.88%（86/118）的受体排卵,排卵时间高度集中,具有较好的推广应用前景。 相似文献
17.
The objective of this study was to determine whether administration of hCG approximately 5 d after AI would increase plasma progesterone concentrations and conception rates in beef heifers. Heifers from two locations (Location 1: n = 347, BW = 367 +/- 1.72 kg; Location 2: n = 246, BW = 408 +/- 2.35 kg) received melengestrol acetate (0.5 mg.heifer(-1).d(-1)) for 14 d and an injection of PGF2alpha (25 mg i.m.) 19 d later. Heifers were observed for estrus continuously during daylight from d 0 to 4.5 after PGF2alpha and artificially inseminated approximately 12 h after the onset of estrus. Half of the heifers inseminated at Location 1 were assigned randomly to receive an injection of hCG (3,333 IU i.m.) 8 d after PGF2alpha, and a blood sample was collected from all heifers 14 d after PGF2alpha for progesterone analysis. Half of the heifers inseminated at Location 2 were administered hCG on d 9 after PGF2alpha, and a blood sample was collected from all heifers 17 d after PGF2alpha. Heifers at Location 1 had a 94% synchronization rate, exhibited estrus 2.45 +/- 0.03 d after PGF2alpha, and received hCG 5.55 +/- 0.03 d after AI. Heifers at Location 2 had an 85% synchronization rate, exhibited estrus 2.69 +/- 0.03 d after PGF2alpha, and received hCG 6.31 +/- 0.03 d after AI. Progesterone concentrations were greater (P < 0.01) for hCG-treated heifers than for controls at both locations (8.6 vs. 4.6 ng/mL for treatment vs. control at Location 1, and 11.2 vs. 5.6 ng/mL for treatment vs. control at Location 2). Pregnancy status was determined by ultrasound approximately 50 d after AI. Conception rates (65 vs. 70% for treatment vs. control, respectively) did not differ at Location 1. Conception rates tended (P = 0.10) to be increased with hCG treatment at Location 2 (61 vs. 50% for treatment vs. control, respectively). A second experiment was conducted with 180 heifers at a third location to determine the effects of hCG administration 6 d after timed insemination at approximately 60 h after PGF2alpha in heifers synchronized as in Exp. 1. Pregnancy rate to timed AI did not differ between hCG-treated (62%) and control heifers (59%). Final pregnancy rate after timed AI and bull exposure (92%) was not affected by treatment. In summary, administration of hCG 5 to 6 d after AI did not improve conception or pregnancy rates at two out of three locations evaluated, suggesting insufficient progesterone is not a major factor contributing to early pregnancy failure in beef heifers. 相似文献
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Progesterone plus PMSG Priming in seasonally anovulatory lactating Sarda ewes exposed to the ram effect 总被引：1，自引：0，他引：1
Todini L Malfatti A Barbato O Costarelli S Debenedetti A 《The Journal of reproduction and development》2007,53(2):437-441
The aim of this trial was to evaluate the effectiveness (fertility and lambing) of priming with a single injection of progesterone plus PMSG in anovulatory lactating Sarda ewes subjected to the ram effect (RE) in spring. Thirty ewes (P4 group) were i.m. injected with 30 mg progesterone and 500 IU PMSG 36 h before ram introduction (d 0). This treatment was compared to a 12-day treatment with fluorogestone acetate intravaginal sponges that was followed by injections of 350 IU PMSG upon sponge withdrawal (FGA group, n=30). All ewes responded to RE, showing plasma progestrone concentrations >1 ng/mL between d 6 and 12 (FGA) or 6 and 9 (P4). Eighty-nine percent of the P4 ewes conceived at first ovulation, and 11% conceived following a short estrus cycle. Lambings occurred on d 150.4 +/- 3.9, and the lambing rate was 100%. The fertility of the FGA ewes was 83% for the induced ovulation and was 7% for the second ovulation after a normal cycle. The FGA ewes lambed on d 149.8 +/- 4.4, and the lambing rate was 83%. Two abortions were recorded for the FGA ewes, which had higher prolificacy than the P4 group (2.2 +/- 0.8 vs. 1.8 +/- 0.4, respectively; P<0.05). Both fertility and the lambing rate were high in both groups, with a high degree of estrus synchronization, and there were no significant differences between the groups. We concluded that priming of lactating Sarda ewes in spring with P4+PMSG before RE is an effective and competitive method (cheaper and more practical than FGA+PMSG) of inducing fertile ovulations in these ewes. 相似文献