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1.
试验借助瘤胃、十二指肠造瘘技术,研究4种蛋白质水平[8.4%(A)、11.2%(B)、14.0%(C)、16.8%(D)]日粮对湖羊胃肠道pH值、氨态氮和菌体蛋白的影响,对科学的指导反刍动物生产具有现实意义。试验结果显示,随着日粮蛋白质水平的升高,湖羊胃肠道各参数也呈现了升高的趋势。瘤胃液氨氮浓度在食后4h达到峰值,蛋白质水平高时产氨量也较高,D组极显著高于其它组(P<0.01);C组极显著高于A组(P<0.01)。十二指肠食糜中D组氨氮浓度最高,极显著高于其它三组(P<0.01);B、C组极显著高于A组(P<0.01)。瘤胃液中的菌体蛋白以D组(5.24mg N/100ml)最高,A组最低(4.18mg N/100ml),D组显著高于A组(P<0.05)。表明蛋白质水平日粮对湖羊胃肠道内环境各参数有显著影响。 相似文献
2.
选取3只体况良好的湖羊,安装永久性瘤胃瘘管、十二指肠近端T型瘘管。采用4×3不完全拉丁方试验设计方案,研究在四种不同蛋白质水平(8.4%(A)、11.2%(B)、14.0%(C)、16.8%(D))日粮下,湖羊胃肠道氨基酸浓度的变化规律。结果显示:①瘤胃液肽氨基酸(PAA)浓度呈现随蛋白质水平升高而升高的趋势,D组极显著高于A、B组(P0.01),显著高于C组(P0.05);C组显著高于A、B组(P0.05)。②十二指肠食糜中,PAA浓度以D组最高,C组次之,C、D组显著高于A、B组(P0.05)。 相似文献
3.
试验选用3只健康装有门静脉血插管的湖羊,采用4×3不完全拉丁方试验设计,研究8.4%(A)、11.2%(B)、14.0%(C)、16.8%(D)四种日粮蛋白质水平下,湖羊门静脉血液生化指标的变化。试验结果表明:(1)血浆氨态氮(NH3-N)浓度以C组最高,极显著高于A、B组(P0.01);D组显著高于A组和B组(P0.05)。(2)血浆尿素氮(PUN)浓度随蛋白质水平的升高而升高,D组极显著高于A、B组(P0.01),C组显著高于A组(P0.05)。(3)血浆肽氨基酸(PAA)浓度以D组最高,C组次之,C、D组显著高于A组(P0.05)。本试验结果提示,日粮蛋白质水平对血液生理指标有显著影响,其中以C组日粮蛋白质水平较适宜。 相似文献
4.
日粮蛋白质水平对湖羊胃肠道pH、氨态氮及尿素氮的影响 总被引：3，自引：1，他引：2
本试验用装有永久性瘤胃瘘管、十二指肠近端T型瘘管的3只湖羊(35±5)kg作为试验动物,按3×3拉丁方试验设计方案,研究了日粮蛋白质水平分别为8.4%(A)、11.2%(B)、14.0%(C)时对湖羊瘤胃和十二指肠pH值、氨态氮、尿素氮浓度的影响。结果表明:①瘤胃pH为6.31~6.43,各组间差异不显著(P>0.05);十二指肠pH为2.64~2.70,各组间差异不显著(P>0.05)。②瘤胃氨态氮浓度(3.24~11.99)mg/100mL,C组极显著高于A、B组,B组极显著高于A组(P<0.01);十二指肠氨态氮浓度(3.01~7.88)mg/100mL,C组极显著高于A、B组,B组极显著高于A组(P<0.01)。③瘤胃尿素氮浓度(13.81~17.51)mg/100mL,C组极显著高于A、B组(P<0.01),B组显著高于A组(P<0.05);十二指肠尿素氮浓度(4.1~5.91)mg/100mL,B、C组极显著高于A组(P<0.01),B组与C组差异不显著(P>0.05)。 相似文献
5.
不同酪蛋白添加水平对湖羊肠系膜排流组织、门静脉排流组织中游离氨基酸和肽结合氨基酸净流量的影响 总被引：1，自引：0，他引：1
本试验旨在研究饲粮不同酪蛋白添加水平对湖羊血浆中游离氨基酸(FAA)和肽结合氨基酸(PAA)释放量及肠系膜排流组织(MDV)、门静脉排流组织(PDV)净流量的影响.选用3只体况良好的湖羊,安装肠系膜静脉、门静脉和颈动脉插管.采用4×3不完全拉丁方试验设计,试验羊分别饲喂4种不同酪蛋白添加水平(A:0、B:3.0%、C:6.0%、D:9.0%)的饲粮.结果显示,湖羊血浆氨基酸浓度随着饲粮酪蛋白添加水平的升高而升高.MDV的FAA净流量以D组最高,且显著高于A组(P<0.05);PAA净流量则是C组最高,且显著高于A、B组(P<0.05).PDV的FAA和PAA净流量都是C组最高,且显著或极显著高于A、B组(P<0.05或P<0.01).结果提示,在一定范围内,饲粮蛋白质水平影响湖羊MDV和PDV中FAA和PAA的净流量. 相似文献
6.
不同SC/NSC日粮对徐淮白山羊瘤胃发酵的影响 总被引：1，自引：0，他引：1
选取4只体况良好、体重(30±2.5)kg的徐淮白山羊,并安装永久性瘤胃瘘管,按4×4拉丁方设计,饲喂4种结构性碳水化合物(SC)与非结构性碳水化合物(NSC)比例不同的日粮(SC/NSC3.52、2.06、1.28、0.84),分别为A、B、C、D组,研究徐淮白山羊瘤胃发酵规律.结果表明D组瘤胃液pH值极显著低于其他3组(P＜0.01);D组瘤胃液氨氮浓度(NH3-N)极显著高于A、B组(P＜0.01);A组微生物蛋白(MCP)浓度极显著低于其他3组(P＜0.01);瘤胃液总挥发性脂肪酸(VFA)、乙酸及丙酸浓度,C组最高,但差异不显著(P＞0.05);D组丁酸浓度极显著高于A、B、C三组(P＜0.01);乙酸与丙酸之比差异不显著(P＞0.05).结果表明,SC/Nsc为1.28时徐淮白山羊瘤胃发酵最佳. 相似文献
7.
日粮系酸力水平对羔羊生产性能和养分表观消化率的影响 总被引：1，自引：0，他引：1
为了研究日粮不同系酸力水平对羔羊生产性能和饲料养分消化率的影响,将24只7日龄萨能奶山羊公羔随机分成4组,A组饲喂基础H粮,B、C和D 3个试验组分别饲喂在基础日粮中添加3.0、9.0和15.0 kg/t酸化剂的试验日粮(补饲料系酸力水平分别为30.0、20.0和10.0 mmol HCI/100 g;日粮系酸力水平分别为39.3、35.2和31.1 mmol HCl/100 g),研究日粮系酸力水平对早期断奶羔羊生产性能和养分表观消化率的影响.结果表明:(1)B、C和D组羔羊日增重极显著高于A组(P<0.01),C、D组显著高于B组(P<0.05);(2)饲料酸化可有效改善羔羊健康状况,B、C和D组羔羊腹泻率分别较A组降低了36.36%、28.65%和81.82%;(3)B,C和D组CP、GE表观消化率极显著高于A组(P<0.01),C、D组极显著高于B组(P<0.01);(4)B、C和D组DM表观消化率极显著高于A组(P<0.01),C、D组极显著高于B组(P<0.01),D组显著高于C组(P<0.05).结果提示,降低羔羊日粮系酸力水平,可以显著改善羔羊日增重、料重比和羔羊对日粮主要养分表观消化率,有效改善羔羊健康状况,在本研究条件下以补饲料系酸力水平10.0 mmol HCl/100 g即日粮系酸力水平31.1 mmol HCl/100 g组效果最好. 相似文献
8.
采用4×4拉丁方试验设计,将8只装有瘤胃瘘管绵羊分为4组;饲粮DCAB采用、(Na K -CI-)meq/100gDM公式计算,按DCAB水平不同分为4个处理,即A(25)、B(35)、C(45)、D(55),结果表明:(1)各处理瘤胃液pH值均在6.21～6.94之间变动,(P>0.05),各组在喂后3h下降到最低点.(2)B、C、D处理瘤胃液氨氮浓度各时间段平均值显著低于A处理(P<0.05);B处理喂后1h、3h、5h、7h NH3-N浓度显著低于A处理(P<0.05);处理NH3-N浓度变化于4.47～49.67mg/100mL之间.(3)B处理瘤胃液尿素氮各时间段浓度平均值显著高于A、C、D处理(P<0.05).(4)各处理瘤胃液乙酸、丙酸、丁酸、TVFA浓度及乙酸/丙酸值各时间段平均值问差异不显著(P>0.05);B、D处理丙酸浓度高于A、C处理(P=0.08),A处理丁酸浓度高于B、C、D处理(P=0.06),B、D处理乙酸/丙酸值低于A、C处理(P=0.07).综合考虑,35 meq/100gDM DCAB水平有利于绵羊瘤胃发酵,该组合为最佳日粮. 相似文献
9.
选择9只18月龄左右安装永久性瘤胃瘘管的重庆黑山羊羯羊,随机分成A、B、C 3组,分别在基础日粮中添加4%百事美脂肪粉、4%菜籽油、0%脂肪饲喂,研究不同形式脂肪对山羊瘤胃消化代谢的影响。结果表明:B组DM降解率极显著高于A、C组(P0.01);但其CP降解率极显著低于A、C组(P0.01),A组极显著低于C组(P0.01)。B组NDF、ADF、ADL降解率极显著低于A、C组(P0.01),A组ADF降解率极显著低于C组(P0.01)。B组pH极显著高于A、C组(P0.01),A组极显著高于C组(P0.01);B组瘤胃液NH3-N浓度极显著低于A、C组(P0.01)。B组血糖浓度极显著高于A、C组(P0.01);B组血清胆固醇浓度极显著低于A、C组(P0.01)。从以上指标来看,在重庆黑山羊日粮中添加百事美脂肪粉效果最好。 相似文献
10.
为研究精氨酸水平对离乳期梅花鹿瘤胃发酵参数和微生物菌群结构的影响,试验选择3月龄,体重相近的12只健康雄性梅花鹿,随机分为3组,每组4只。仔鹿饲喂蛋白质水平为12.28%的低蛋白质饲粮,精氨酸水平分别为0.80%(A组)、1.08%(B组)、1.26%(C组),试验期50 d。在试验期的最后1 d抽取仔鹿瘤胃液20 mL,一部分用于测定瘤胃发酵参数,一部分用于提取DNA分析瘤胃细菌多样性。结果表明：1)B组氨态氮含量极显著高于C组（P<0.01）;B组乙酸、丁酸、异丁酸含量极显著低于A组（P<0.01）,总挥发性脂肪酸含量显著低于A组（P<0.05）;B组的乙丙比极显著低于A、C组（P<0.01）。2)B组ACE指数显著高于C组（P<0.05）,Chao1指数显著高于A、C组（P<0.05）,B组Simpson指数极显著高于A、C组（P<0.01）,Shannon指数则极显著低于A、C组（P<0.01）,A组细菌群落结构与B、C组差异显著（P<0.05）。3）在门水平上,B组拟杆菌门（Bacteroidetes）相对丰度极显著低于A... 相似文献
11.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献12.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献14.
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Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
18.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献19.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
20.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献