共查询到20条相似文献，搜索用时 62 毫秒
1.
Rapid endothelialization is important for biodegradable blood-contacting devices not only to prevent thrombosis but also to prevent degradation debris from entering the bloodstream and causing further complications. Here the authors report a three-step surface modification method, by which biomolecules, such as gelatin and chitosan, are covalently immobilized on the surface of plasma-treated poly(L-lactic acid) (PLLA) via -COOH groups introduced by acrylic acid grafting polymerization. Surface characterization techniques, including x-ray photoelectron spectroscopy, contact angle measurement, and colorimetric methods for surface density of functional groups, proved the feasibility and stability of this surface modification method. Surface wettability was increased by biomolecules immobilization. The -COOH surface density was measured to be 4.17±0.15?μmol/cm(2), the and amount of gelatin immobilized was 4.8?μg/cm(2). Human umbilical vein endothelial cell was used during in vitro study at seeding density of 10(4)?cells/cm(2). PLLA-gAA-gelatin surface was found to enhance cell adhesion, spreading, focal adhesion formation, and proliferation significantly. Chitosan-modified PLLA shows marginally improvement in cell adhesion and proliferation. Endothelialization was achieved within 7 days on both modified PLLA surfaces. In conclusion, this work demonstrates the feasibility of the surface modification method, and its ability to promote complete endothelialization for cardiovascular applications. 相似文献
2.
Christophis C Sekeroglu K Demirel G Thome I Grunze M Demirel MC Rosenhahn A 《Biointerphases》2011,6(4):158-163
Nanotextured polymeric surfaces with inclined rods reveal highly anisotropic properties concerning wetting and adhesion. In this work, we report on the interaction of fibroblast cells with these highly anisotropic materials. The authors quantified removal of adherent cells from such surfaces by a laminar flow. The critical shear force needed for cell removal from the surface depends on the inclination direction. Based on electron microscopy cross sections we deduce that interactions of cellular filopodia extending into the nanotextured surface are causing the direction depending removal. 相似文献
3.
To enhance the cytocompatibility of polycaprolactone (PCL), cell-adhesive gelatin is covalently immobilized onto the PCL film surface via two surface-modified approaches: a conventional chemical immobilization process and a surface-initiated atom transfer radical polymerization (ATRP) process. Kinetics studies reveal that the polymer chain growth from the PCL film using the ATRP process is formed in a controlled manner, and that the amount of immobilized gelatin increases with an increasing concentration of epoxide groups on the grafted P(GMA) brushes. In vitro cell adhesion and proliferation studies demonstrate that cell affinity and growth are significantly improved by the immobilization of gelatin on PCL film surfaces, and that this improvement is positively correlated to the amount of covalently immobilized gelatin. With the versatility of the ATRP process and tunable grafting efficacy of gelatin, this study offers a suitable methodology for the functionalization of biodegradable polyesters scaffolds to improve cell-material interactions. 相似文献
4.
Kutlay SeverMehmet Sarikanat Yolda? SekiGökhan Erkan Ümit Halis Erdo?anSeckin Erden 《Industrial Crops and Products》2012,35(1):22-30
In this study, jute fabrics were modified by alkali, micro-emulsion silicon (MS) and fluorocarbon based agents (FA) in order to enhance the interfacial adhesion between the polyester matrix and the jute fiber. X-ray photoelectron spectroscopy (XPS) and contact angle measurements were used to characterize fiber surfaces. The effects of various surface treatments on the mechanical and morphological of jute/polyester composites were also studied. All surface treatments were shown to improve the tensile, flexural strengths and interlaminar shear strengths of the composites. Moreover, the maximum improvement in the mechanical properties was obtained for the FA treated jute/polyester composites. SEM micrographs of the tensile fracture surface of jute/unsaturated polyester composites also exhibited improvement of interfacial and interlaminar shear strengths by the alkali, MS and FA treatments of jute fibers. 相似文献
5.
Cherry JF Carlson AL Benarba FL Sommerfeld SD Verma D Loers G Kohn J Schachner M Moghe PV 《Biointerphases》2012,7(1-4):22
This article focuses on elucidating the key presentation features of neurotrophic ligands at polymer interfaces. Different biointerfacial configurations of the human neural cell adhesion molecule L1 were established on two-dimensional films and three-dimensional fibrous scaffolds of synthetic tyrosine-derived polycarbonate polymers and probed for surface concentrations, microscale organization, and effects on cultured primary neurons and neural stem cells. Underlying polymer substrates were modified with varying combinations of protein A and poly-D-lysine to modulate the immobilization and presentation of the Fc fusion fragment of the extracellular domain of L1 (L1-Fc). When presented as an oriented and multimeric configuration from protein A-pretreated polymers, L1-Fc significantly increased neurite outgrowth of rodent spinal cord neurons and cerebellar neurons as early as 24 h compared to the traditional presentation via adsorption onto surfaces treated with poly-D-lysine. Cultures of human neural progenitor cells screened on the L1-Fc/polymer biointerfaces showed significantly enhanced neuronal differentiation and neuritogenesis on all protein A oriented substrates. Notably, the highest degree of βIII-tubulin expression for cells in 3-D fibrous scaffolds were observed in protein A oriented substrates with PDL pretreatment, suggesting combined effects of cell attachment to polycationic charged substrates with subcellular topography along with L1-mediated adhesion mediating neuronal differentiation. Together, these findings highlight the promise of displays of multimeric neural adhesion ligands via biointerfacially engineered substrates to "cooperatively" enhance neuronal phenotypes on polymers of relevance to tissue engineering. 相似文献
6.
Tethered lipid membranes or immobilized lipid vesicles are frequently used as biomimetic systems. In this article, the authors presented a suitable method for efficient immobilization of lipid vesicles onto a broad range of surfaces, enabling analysis by quantitative methods even under rigid, mechanical conditions-bare surfaces such as hydrophilic glass surfaces as well as hydrophobic polymer slides or metal surfaces such as gold. The immobilization of vesicles was based on the electrostatic interaction of zwitterionic or negatively charged lipid vesicles with two types of cationic chemically modified bovine serum albumin (cBSA) blood plasma proteins (cBSA-113 and cBSA-147). Quantitative analysis of protein adsorption was performed as the cBSA coatings were characterized by atomic force microscopy, surface zeta potential measurement, fluorescence microscopy, and surface plasmon spectroscopy, revealing a maximal surface coverage 270-280?ng/cm(2) for 0.02 mg/ml cBSA on gold. Small unilamellar vesicles as well as giant unilamellar vesicles (GUVs) were readily immobilized (～15?min) on cBSA coated surfaces. GUVs with 5-10 mol% negatively charged 1,2,-dipalmitoyl-sn-glycero-3-phosphoglycerol remained stable in liquid for at least 5 weeks. 相似文献
7.
BN Lourenço G Marchioli W Song RL Reis CA van Blitterswijk M Karperien A van Apeldoorn JF Mano 《Biointerphases》2012,7(1-4):46
Surface wettability and topography are recognized as critical factors influencing cell behavior on biomaterials. So far only few works have reported cell responses on surfaces exhibiting extreme wettability in combination with surface topography. The goal of this work is to study whether cell behavior on superhydrophobic surfaces is influenced by surface topography and polymer type. Biomimetic superhydrophobic rough surfaces of polystyrene and poly(L-lactic acid) with different micro/nanotopographies were obtained from smooth surfaces using a simple phase-separation based method. Total protein was quantified and showed a less adsorption of bovine serum albumin onto rough surfaces as compared to smooth surfaces of the same material. The mouse osteoblastic MC3T3-E1 cell line and primary bovine articular chondrocytes were used to study cell attachment and proliferation. Cells attached and proliferate better in the smooth surfaces. The superhydrophobic surfaces allowed cells to adhere but inhibited their proliferation. This study indicates that surface wettability, rather than polymer type or the topography of the superhydrophobic surfaces, is a critical factor in determining cell behavior. 相似文献
8.
以豫82×沈137衍生的一套F_(10)家系为材料,采用2个环境、2种密度、3个重复的田间试验设计,对玉米8个株型相关性状(叶夹角、叶长、叶宽、叶向值、叶基至最高点距离、穗上部各节间距离、株高、穗位高)进行表型鉴定。联合方差分析结果表明,叶夹角、穗上节间距、株高、穗位高4个性状基因型×密度间互作差异达显著水平,说明基因型与密度间互作是影响变异的主要因素,可将4个性状作为判断玉米株型耐密性指标。两种密度处理比较结果表明,随着密度的增加,4个性状平均值也随着增加。将两种密度条件下性状平均值比较,可将190个家系划分为密度敏感型、较敏感型、中间型和钝感型4种类型。 相似文献
9.
不同密度下玉米雌雄穗开花间隔与产量关系研究 总被引：2，自引：1，他引：1
以70年代以来我国大面积推广的杂交种为材料,在15 000(低)、45 000(中)、75 000(高)株/hm2密度下,研究不同密度对不同年代杂交种雌雄穗开花期间隔(ASI)、产量及相关性状的影响。结果表明,随着密度增加,ASI与产量关系更趋密切。高密度下,ASI与产量显著正相关;中、高密度下不同年代品种间ASI差异显著,且高密度下ASI、产量与年代呈显著直线回归关系。随密度增加,新品种ASI增加变化率减小,但产量增加变化率较大。ASI值可作为品种耐密性鉴定的重要参考指标,不同密度下新品种ASI更趋稳定,耐密性增强。 相似文献
10.
The standard method for culturing human embryonic stem cells (hESC) uses supporting feeder layers of cells or an undefined substrate, Matrigel(?), which is a basement membrane extracted from murine sarcoma. For stem cell therapeutic applications, a superior alternative would be a defined, artificial surface that is based on immobilized human plasma vitronectin (VN), which is an adhesion-mediating protein. Therefore, VN adsorbed to diverse polymer surfaces was explored for the continuous propagation of hESC. Cells propagated on VN-coated tissue culture polystyrene (TCPS) are karyotypically normal after >10 passages of continuous culture, and are able to differentiate into embryoid bodies containing all three germ layers. Expansion rates and pluripotent marker expression verified that a minimal VN surface density threshold is required on TCPS. Further exploration of adsorbed VN was conducted on polymer substrates with different properties, ranging from hydrophilic to hydrophobic and including cationic and anionic polyelectrolyte coatings. Despite differing surface properties, these substrates adsorbed VN above the required surface density threshold and were capable of supporting hESC expansion for >10 passages. Correlating wettability of the VN-coated surfaces with the response of cultured hESC, higher cell expansion rates and OCT-4 expression levels were found for VN-coated TCPS, which exhibits a water contact angle close to 65°. Importantly, this simple, defined surface matches the performance of the benchmark Matrigel, which is a hydrogel with highly complex composition. 相似文献
11.
12.
种植密度对青贮玉米品种产量及相关性状的影响 总被引：3，自引：0，他引：3
以6个青贮玉米品种为试验材料,研究了3种种植密度对青贮玉米生物产量、干物质产量及相关性状的影响。结果表明,不同品种之间生物产量和干物质产量差异极显著;不同密度下品种的生物产量和干物质产量差异极显著;品种与密度互作之间生物产量和干物质产量差异分别为显著和极显著。龙育1号、高油169和高油115作为优质青贮高油玉米在黑龙江省适宜种植密度分别为7.0～8.0万株/hm2、7.0万株/hm2和6.0万株/hm2;黑饲1号在6万株/hm2时产量较高;龙辐208在7万株/hm2种植条件下,其生物产量和干物质产量均较高,尤其干物质产量最高;青贮玉米品种的青贮生育日数、株高、穗位、茎粗、收获期绿叶片数差异不大,品种之间差异主要由品种自身特性决定。中原单32可作为粒用玉米种植推广。 相似文献
13.
A. Elgersma G. Ellen† H. van der Horst‡ B. G. Muuse† H. Boer‡ S. Tamminga‡ 《Grass and Forage Science》2003,58(3):323-331
Two experiments were conducted to evaluate the effects of cultivar and season on the fatty acid (FA) composition of the lipids of perennial ryegrass (Lolium perenne L.). Eight diploid cultivars were cut at the same target yield of approximately 2000 kg dry matter ha?1 between mid‐June and mid‐September. Two cultivars (Barlet and Magella) were harvested during four 2‐week periods and six cultivars (AberGold, Respect, Agri, Herbie, Barezane and Barnhem; cultivars 1–6) during three periods. The concentrations of individual FA were determined by gas chromatography. Barlet had higher concentrations of linolenic acid (C18:3) than Magella, but lower concentrations of linoleic acid (C18:2). Cultivars 1–6 were more variable in their leaf blade and stem proportions than Barlet and Magella. Despite this, there was no difference between cultivars 1–6 in the FA composition of the herbage. On average 0·74 of the FA consisted of C18:3. Higher concentrations of total FA were found in mid‐summer than in early summer. This was related to a high leaf blade proportion in the herbage, indicating that the proportion of leaf and stem of the herbage probably had more effect on lipid concentrations than the season per se in this period. However, in late August and mid‐September, the total FA concentration declined whereas the leaf blade proportion increased. Therefore, in this period environmental factors appeared to have a modifying effect. As consistent differences in the concentration of C18:3 were found among cultivars Barlet and Magella throughout the season, these studies demonstrate opportunities to change the composition of ruminant products through the choice of cultivars of perennial ryegrass. 相似文献
14.
Based on the situ preparation of silica nanoparticles (SiO2) on the surface of Graphene nanoplatelets (GNPs) in the previous work, these unique three dimensional (3D) materials were introduced into epoxy resin to study the reinforcing and toughening synergy effect on the composites. Firstly, the tensile tests showed that Graphene/SiO2 hybrid materials attached with different size of SiO2 particles exhibited different reinforcing and toughening effect on the composites. With the increasing of the diameter of SiO2 particles, the toughness and strength properties of the composites firstly improved and then decreased, and when the average diameter was 0.14 μm, the elongation reached the max.. Meanwhile, the fractured surfaces presented on SEM images were consistent with the results of the tensile tests, which further explained the hybrid materials increased the interfacial adhesion between the fillers and matrix, leading to significant improvement in mechanical properties. Moreover, the DSC curves demonstrated that Graphene/SiO2 hybrid materials accelerated the curing process of epoxy resin due to the cross-link structure between fillers and matrix. Lastly, the crack propagation modes were built to clarify the synergy effect mechanism of reinforcing and toughening on nanoparticles/epoxy resin composites. 相似文献
15.
Adhesion of red blood cells (RBCs) to endothelial cells (ECs) is usually insignificant but an enhanced adhesion has been observed in various diseases associated with vascular complications. This abnormal adhesion under pathological conditions such as sickle cell disease has been correlated with increased levels of various plasma proteins but the detailed underlying mechanism(s) remains unclear. Usually it is assumed that the proadhesive effects of plasma proteins originate from ligand interactions cross-linking receptors on adjacent cells, but explicit results detailing binding sites or receptors for some proteins (e.g., fibrinogen) on either RBC or EC surfaces that would support this model are missing. In this study, the authors tested whether there is an alternative mechanism. Their results demonstrate that dextran 2 MDa promotes the adhesion of normal RBCs to thrombin-activated ECs and that this effect becomes more pronounced with increasing thrombin concentration or with prolonged thrombin incubation time. It is concluded that depletion interaction originating from nonadsorbing macromolecules (i.e., dextran) can modulate the adhesion of red blood cells to thrombin-activated EC. This study thereby suggests macromolecular depletion as an alternative mechanism for the adhesion-promoting effects of nonadsorbing plasma proteins. These findings should not only aid in getting a better understanding of diseases associated with vascular complications but should also have many potential applications in biomedical or biotechnological areas that require the control of cell-cell or cell surface interactions. 相似文献
16.
设置两年灌溉量和种植密度互作试验，研究不同密度下，随灌溉量增加，玉米茎秆抗折断力、植株高度、基部节间形态及机械强度等的变化趋势，明确覆膜滴灌条件下灌溉量对密植玉米茎秆抗倒伏能力的影响。结果表明，随密度增加，茎秆抗倒能力呈下降趋势。不同密度下，滴灌量变化对茎秆抗倒能力的影响不完全一致。密植条件下，适当增加灌量（480～540 mm）可提高基部节间的物质积累和茎粗，增强茎秆的机械强度；继续增加滴灌量对茎秆的抗倒能力提升效果不显著。 相似文献
17.
J. Vos 《Potato Research》1995,38(3):271-279
Summary The interaction between nitrogen supply and stem density on leaf attributes and branching was studied in two field experiments.
Stem densities included 8, 16, 24, 32 and 40 stems m−2. The low-N treatments received 5 or 10 g m−2 nitrogen, and the high-N treatments 20 or 25 g m−2.
The sizes of mature individual leaves on the main stem declined with increase in stem density and were larger for greater
N supply, with additive effects of nitrogen and stem density. The specific leaf area was not affected by nitrogen supply and
increased with stem density (range of values: 210 to 310 cm2 g−1). The leaf weight ratio was not affected by the treatments. At 8 and 16 stems m−2 basal branches contributed substantially to the total leaf area per stem: at 24 stems m−2 and higher densities the development of basal branches was negligible. Leaf area on apical branches was always smaller for
higher plant densities and lower rates of N supply. 相似文献
18.
Antimicrobial surfaces for food and medical applications have historically involved antimicrobial coatings that elute biocides for effective kill in solution or at surfaces. However, recent efforts have focused on immobilized antimicrobial agents in order to avoid toxicity and the compatibility and reservoir limitations common to elutable agents. This review critically examines the assorted antimicrobial agents reported to have been immobilized, with an emphasis on the interpretation of antimicrobial testing as it pertains to discriminating between eluting and immobilized agents. Immobilization techniques and modes of antimicrobial action are also discussed. 相似文献
19.
The response of Russet Burbank grown at five plant densities (4.0 to 11.1 × 104 plants ha?1), to level of fertilization was studied in field experiments at Guelph, Canada in the 1972 and 1973 growing seasons. Growth analysis techniques were used to establish growth patterns of Russet Burbank at three of the above plant densities (4.0,6.3 and 11.1 × 104plants ha?1), while the effect on root development of rate of fertilization was observed in controlled environments. In both seasons total tuber yield was not influenced by plant density, while marketable yield showed a negative response to increased density. The interaction between plant density and level of fertilization did not approach significance in either season. Due to greater axillary branching at the lower plant densities, leaf area index and photosynthetically active radiation measured at two levels in the canopy, were similar at all densities. Fertilizer levels used in the field showed no negative effect on root growth in experiments conducted in controlled environments. Marked variation in the number of mainstems and thus the number of tubers produced per plant between seasons indicated the importance of using the mainstem as the basic population unit. 相似文献
20.
杉木林下南方红豆杉初期生长分析 总被引：2，自引：0，他引：2
调查研究了福建省大田县杉木林下南方红豆杉的初期生长（树高、地径和冠幅）差异。结果表明：3个指标在不同郁闭度、地类及郁闭度与地类交互作用条件下均存在极显著差异。不同郁闭度的杉木林下树高、地径和冠幅平均值大小次序均为郁闭度0.6〉郁闭度0.7〉郁闭度0.5〉郁闭度0.8,表明适当的光照有利于南方红豆杉树高、地径和冠幅的生长,郁闭度过大或过小均对树木生长不利,以郁闭度0.6-0.7为佳。不同地类的杉木林下树高、地径和冠幅平均值大小次序均为Ⅰ类立地〉Ⅱ类立地〉Ⅲ类立地,Ⅰ类、Ⅱ类立地有利于南方红豆杉生长,以Ⅰ类立地为佳。不同郁闭度与地类组合的杉木林下树高、地径和冠幅平均值较大的前3种组合均为郁闭度0.6×Ⅰ类立地、郁闭度0.7×Ⅰ类立地和郁闭度0.6×Ⅱ类立地,表明在考虑郁闭度与地类交互作用的条件下,郁闭度0.6-0.7、Ⅰ类立地最有利于南方红豆杉生长。 相似文献