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1.
为了解三峡水库库尾地区面源污染现状,以重庆市江津区笋溪河流域为研究对象,利用综合水质识别指数、内梅罗指数和主成分分析方法对笋溪河水质进行评价,主要评价指标有水温、pH、电导率、溶解氧、化学需氧量、总氮和总磷。结果表明:(1)笋溪河流域溶解氧为5.98～8.70 mg/L、化学需氧量为2～14 mg/L,总磷为0.008～0.105 mg/L,均处于Ⅰ类或Ⅱ类水质,总氮为0.64～2.03 mg/L,处于Ⅲ～Ⅴ类水质,污染严重。(2)3种水质评价方法均显示下游水质劣于上游水质,内梅罗指数在考虑平均超标水平的同时兼顾最大超标污染物,研究认为该指数的评价结果最能表现该地区的水质现状。(3)笋溪河上下游之间,溶解氧为上游显著高于下游,水温、pH、电导率、化学需氧量和总氮均为下游显著高于上游(P0.05),总磷浓度未发现有明显的差异(P0.05)。在后续管理中应提升城镇污水和农村污水的处理率,提高水肥利用率,以消减污染负荷、控制水污染趋势,从而改善区域水环境质量。 相似文献
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汉丰湖入湖支流浮游植物群落结构及环境因子影响分析 总被引：2，自引：0，他引：2
为了准确反映汉丰湖的水环境状况,通过浮游植物监测和环境因子监测相结合,探讨汉丰湖入湖支流东河、桃溪河、南河、头道河浮游植物群落结构特征及其与水体理化因子的关系,对小江中上游开县汉丰湖4条主要支流的水体理化指标和浮游植物进行了采样分析,在东河、南河、桃溪河、头道河各设置3个监测点,于2013年3、6、9、12月各采样1次。结果表明,4条入湖支流共采集鉴定浮游植物8门、84属、218种（含变种）,以硅藻（107种）和绿藻（64种）种类居多,其次是蓝藻（31种）,这3类的种数占浮游植物总数的92.7%,其它5门的种类数较少。各支流浮游植物种类数由低到高依次为东河（83种）、桃溪河（100种）、南河（106种）、头道河（120种）,其密度均值在105数量级,南河下游浮游植物密度在夏季出现最大峰值,达到1.06×107 个/L,在大部分时段均以蓝藻占优势。水体理化指标监测数据显示,汉丰湖4条主要支流的总氮、总磷、pH、溶氧、水温、CODMn和电导率均值分别为1.00~1.61 mg/L、0.03~0.11 mg/L、7.82~8.53、6.65~7.03 mg/L、17.9~20.4℃、2.07~4.57 mg/L、269.25~522.50 μS/cm;其中,南河的总氮、CODMn较高,头道河的总磷较高,各支流的水温、溶氧、pH较为接近。浮游植物与环境因子的相关性分析表明,东河的裸藻、桃溪河的绿藻以及头道河的蓝藻密度均与水温显著相关（P<0.05）,南河的硅藻和绿藻密度与水温极显著正相关（P<0.01）,东河的甲藻密度与总磷浓度极显著相关（P<0.01）,桃溪河的硅藻和甲藻密度与总磷浓度显著相关（P<0.05）,绿藻密度与总氮浓度显著负相关（P<0.05）,硅藻密度与总氮浓度极显著负相关（P<0.01）;浮游植物分布及密度主要受水温、总氮和总磷的影响。 相似文献
3.
《水生态学杂志》2014,(6)
为了准确反映汉丰湖的水环境状况,通过浮游植物监测与环境因子监测相结合,探讨小江中上游汉丰湖入湖支流东河、桃溪河、南河、头道河浮游植物群落结构特征及其与水体理化因子的关系,对水体理化指标和浮游植物进行了采样分析,在东河、南河、桃溪河、头道河各设置3个监测点,于2013年3、6、9、12月各采样1次。结果表明,4条入湖支流共采集鉴定浮游植物8门、84属、218种(含变种),以硅藻(107种)和绿藻(64种)居多,其次是蓝藻(31种),这3类的种数占浮游植物总数的92.7%,其它5门的种类数较少。各支流浮游植物种类数由低到高依次为东河(83种)、桃溪河(100种)、南河(106种)、头道河(120种),其密度均值在105数量级,南河下游浮游植物密度在夏季出现最大峰值,达到1 160×104个/L,在绝大部分时段均以蓝藻占优势。水体理化指标监测数据显示,汉丰湖4条主要支流的总氮、总磷、p H、溶氧、水温、CODMn和电导率均值分别为1.00~1.61 mg/L、0.03~0.11 mg/L、7.82~8.53、6.65~7.03 mg/L、17.9~20.4℃、2.07~4.57 mg/L、269.25~522.50μS/cm;其中,南河的总氮、CODMn较高,头道河的总磷较高,各支流的水温、溶氧、p H较为接近。浮游植物与环境因子的相关性分析表明,东河的裸藻、桃溪河的绿藻以及头道河的蓝藻密度均与水温显著相关(P0.05),南河的硅藻和绿藻密度与水温极显著相关(P0.01),东河的甲藻密度与总磷浓度极显著相关(P0.01),桃溪河的硅藻和甲藻密度与总磷浓度显著相关(P0.05),绿藻密度与总氮浓度显著负相关(P0.05),硅藻密度与总氮浓度极显著负相关(P0.01);浮游植物分布及密度主要受水温、总氮和总磷的影响。 相似文献
4.
2018年春季(5月),在呼伦湖及其周围水域不同类型水体中设置28个采样点(呼伦湖湖域17个,周围河流流域11个)调查了浮游植物种类和数量,监测了水环境因子,探讨了呼伦湖及其周围水域春季浮游植物功能群及其与环境因子之间的关系。结果表明:春季在呼伦湖及其周围水域中共鉴定出7门80种浮游植物,其中呼伦湖中以绿藻门为主,而周围河流流域则以硅藻门为主。调查区域浮游植物共分为20个功能群,其中C、X2、J和D为重要功能群。Pearson线性相关分析和RDA分析均表明:化学需氧量和电导率是影响呼伦湖及其周围水域浮游植物功能群最主要的水环境因子,功能群C和D同化学需氧量和电导率显著负相关,功能群J同化学需氧量和电导率显著正相关,而功能群X2受水温和溶解氧影响较大,同水温和溶解氧显著正相关。用GB3838-2002评价标准评估各采样点的总氮、总磷及化学需氧量结果表明,呼伦湖内(M9、M16)水质极差,总氮、总磷和化学耗氧量均超过国家V类水标准(2mg/L、0.2mg/L、40mg/L);呼伦湖内西岸(M10～M13)、湖中心(M22～M24)以及周围水域的呼和诺尔(M3)、乌兰诺尔(M27)水中总磷和化学需氧量污染极为严重。结果发现,周围河流水质明显优于呼伦湖内水质。 相似文献
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为掌握南水北调东线南四湖的水质现状，于2021年对4个湖区7个站位进行了周年调查，并采用单因子分析和综合营养状态指数法对水质状况进行了评价。结果表明：南四湖水体氨氮和亚硝氮浓度分别为0.166±0.015mg/L和0.050±0.009mg/L,总氮、总磷和CODMn浓度分别为1.767±0.191mg/L、0.053±0.005mg/L和10.58±0.46mg/L并分别超地表水III类水质标准76.69%、6.76%和76.41%;综合营养状态指数介于46.78～66.16,南四湖总体为轻度富营养状态，但部分水域仍存在较重的富营养化问题。伴随南水北调东线的常态化调水及多种水质涵养措施的实施，南四湖水体的富营养状况有所转好，但在部分季节的部分区域仍存在富营养化风险。 相似文献
6.
《淡水渔业》2019,(6)
为了解乌杨调节坝正式运行初期汉丰湖水体情况,2016年10月—2017年10月对汉丰湖浮游植物群落及水质状况进行了调查。结果显示,调查期间共检出浮游植物7门58属114种,其中绿藻门和硅藻门最多,分别占39.47%和38.60%。尖针杆藻、梅尼小环藻和啮蚀隐藻等为优势种。浮游植物平均密度为8.64×10~5 ind./L;平均生物量为2.03 mg/L;其中,平均密度和生物量在2016年12月最小,2017年2—8月不断增大,10月份有所下降;四个采样点的平均密度和生物量差异均不显著。各采样点浮游植物Margalef物种丰富度指数(d_(Ma))为1.69～5.23;Shannon-Wiener指数(H’e)为1.12～2.75;Pielou均匀性指数(J_e)为0.45～0.96。水温、高锰酸盐指数、总磷、总氮、氨氮的变化范围分别为12.7～31.5℃、1.54～4.92 mg/L、0.01～0.18 mg/L、0.20～1.11mg/L、0.10～0.81 mg/L。冗余分析(RDA)结果表明,汉丰湖浮游植物与高锰酸盐指数、总磷、亚硝态氮和氨氮呈正相关,与透明度呈负相关。 相似文献
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根据2010年3月－12月对三峡水库小江沿岸水体的水质监测数据,分析总氮、总磷、化学需氧量和叶绿素a等水质因子的时空变化规律,以及叶绿素a与水质因子之间的相互关系,评价水体富营养化水平。结果表明：总氮、总磷、化学需氧量和叶绿素 a含量分别为1.980±0.119mg/L、0.114±0.018mg/L、9.520±1.748 mg/L和23.342±8.810 mg/L,小江沿岸水体呈现中度富营养化水平。叶绿素a与水质因子间的相关关系分析发现,叶绿素a含量与温度、pH、溶解氧、亚硝酸氮和化学需氧量显著相关,与总氮、总磷不具有显著相关性。这说明小江沿岸水体叶绿素a含量与总营养盐无关,而主要与水体所含的有机质含量有关。 相似文献
8.
研究东圳水库水质变化趋势,计算工程实施后的污染物削减量,为水质保护提供依据。利用SMS模型基于二维不可压缩流体运动方程,建立东圳水库水域二维水流水质数学模型,采用有限单元法,模拟预测东圳水库水环境状况,主要水质分析指标为高锰酸盐指数、氨氮、总氮和总磷。预测结果表明,按目前污染物排放状况,2018年东圳水库水体中污染物含量为氨氮0.14~0.21 mg/L(II类水质)、COD 3.3~3.9 mg/L(III类水质)、总磷0.052~0.062 mg/L(IV类水质),总氮1.1~1.6 mg/L(IV类水质)。实施社会经济调控、污染源防治和生态保育工程后,2018年,预计东圳水库高锰酸盐指数、氨氮、总氮和总磷共排放1 616.6、163.7、1 182.4和155.5 t/a,分别削减了1 501.8、439.9、756.4和105.2 t/a;总氮0.4~0.8 mg/L、总磷0.022~0.032 mg/L,降幅均达到50%以上,达III类水质标准;COD 2.0~3.0 mg/L、氨氮0.11~0.18 mg/L,达到II类水质标准。提出的实施方案对保护东圳水库的水质具有建设性意义,水体水质目标可达。 相似文献
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11.
Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
14.
Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
15.
Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 
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下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
16.
The endemic, anadromous cyprinidChalcalburnus tarichi is the only fish species known to occur in alkaline Lake Van (Eastern Anatolia, Turkey). EightC. tarichi were maintained individually in Lake Van water (17 – 19°C; pH 9.8; 153 mEq·I–1 total alkalinity; 22 total salinity) and tank water samples analyzed for 24 h in 2 to 4 h intervals. At zero time, < 1µM ammonia was present and urea was undetectable in the tank water; at 24 h, total ammonia and urea made up 114±32 and 35±25µM, respectively. Over the experimental period, ammonia-N and urea-N excretion averaged 1041±494 and 607±169moles·kg–1 fish·h–1, respectively. The extent of urea excretion was highly variable between specimens. Uric acid excretion was not detectable.Urea was present at high concentrations in all tissues and plasma (25 – 35moles·g–1·ml–1) of freshly caughtC. tarichi; total ammonia content of the tissues was by a factor of 1.9 (liver) to 3.0 (brain) lower. High arginase activity (2.4±0.2 U·min–1·g–1) was detected in the liver ofC. tarichi but ornithine carbamoylphosphate transferase, a key enzyme of the ornithine-urea-cycle, was absent. Ureagenesis is likely through degradation of arginine and/or uricolysis. High glutamine synthetase activity (11±0.6 U·min–1·g–1) and low ammonia content in brain suggest that, like other teleosts,C. tarichi has an efficient ammonia detoxification in the brain, but in no other tissue.Nitrogenous waste excretion at alkaline pH is discussed. The ability ofC. tarichi to excrete high levels of ammonia at extremely alkaline pH is unique among teleosts studied so far. The mechanism of ammonia excretion under Lake Van conditions remains to be elucidated. 相似文献
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There has been growing concern about the overuse of antibiotics in the ornamental fish industry and its possible effect on the increasing drug resistance in both commensal and pathogenic organisms in these fish. The aim of this study was to carry out an assessment of the diversity of bacteria, including pathogens, in ornamental fish species imported into North America and to assess their antibiotic resistance. Kidney samples were collected from 32 freshwater ornamental fish of various species, which arrived to an importing facility in Portland, Oregon from Colombia, Singapore and Florida. Sixty‐four unique bacterial colonies were isolated and identified by PCR using bacterial 16S primers and DNA sequencing. Multiple isolates were identified as bacteria with potential to cause disease in both fish and humans. The antibiotic resistance profile of each isolate was performed for nine different antibiotics. Among them, cefotaxime (16% resistance among isolates) was the antibiotic associated with more activity, while the least active was tetracycline (77% resistant). Knowing information about the diversity of bacteria in imported ornamental fish, as well as the resistance profiles for the bacteria will be useful in more effectively treating clinical infected fish, and also potential zoonoses in the future. 相似文献
18.
Effect of iodophor disinfection of non‐hardened Salmo trutta eggs on their bacterial and fungus load 下载免费PDF全文
下载免费PDF全文 Franz Lahnsteiner 《Aquaculture Research》2017,48(7):3901-3909
This study investigated the efficiency of iodophor disinfection (135 ppm active iodine for 15–30 min) of non‐hardened Salmo trutta eggs against different groups of bacteria and against fungus. Egg samples were taken from non‐disinfected and from disinfected eggs, microorganisms were cultured on specific nutrient media and their mass was measured by turbidimetric methods. Bacteria and fungus mass of non‐hardened eggs could be reduced but not eliminated by iodophor disinfection with 135 ppm active iodine for 15 min. The extent of reduction was 47–65% (Experiment 1). The efficiency of disinfection increased with disinfection time as the reduction in bacteria and fungus mass was 40–55% after 15 min and 58–74% after 30 min (Experiment 2). Disinfection efficiency of iodophor solution diluted in water (reduction 49–57%) and of iodophor solution diluted in sodium chloride solution iso‐osmolar to the oocytes (reduction 52–61%) was similar (Experiment 3). The reduction in bacteria and fungus mass was persistent as it was 39–72% lower in embryos deriving from disinfected eggs than in embryos deriving from non‐disinfected ones (Experiment 4). In conclusion, the tested disinfection method is inadequate to eliminate pathogens completely but it could positively influence immune defence of eggs and embryos. 相似文献
19.
T. Citarasu K. Venkatramalingam M. Micheal Babu R. Raja Jeya Sekar M. Petermarian 《Aquaculture International》2003,11(6):581-595
The indiscriminate use of antibiotics and chemicals in shrimp hatcheries has led to biomagnification and that in turn could
lead to rejection of a whole consignment. The application of the bioencapsulation technique as a tool for curative treatment
in shrimp larvae was investigated. Herbs having antibacterial properties such as Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia (methanolic extracts) were bioencapsulated in Artemia and fed to Penaeus monodon post larvae PL 1–25. The post larvae were reared in a medium inoculated with pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Vibrio sp. Post larvae reared in the non-inoculated water and fed with non-enriched Artemia exhibited 90% survival, highest specific growth rate (12.43%) and reduced bacterial load. P. monodon reared in the bacterial inoculated water and fed with the non-enriched Artemia exhibited the lowest survival (10–30%), specific growth rate (8.42–9.1%) and increased bacterial load (2.86 × 103 to 3.76 × 105 cfu/g). The methanolic extracts of the herbs helped to increase survival and specific growth rate and reduced bacterial load
in the P. monodon culture system. Among the three herbal extracts, P. corylifolia enriched Artemia fed post larvae showed the tendency to higher survival (>50%), growth rate (11.5 averaged) and low bacterial load (1.12 × 105 cfu/g).
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献