共查询到19条相似文献，搜索用时 250 毫秒
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[目的]为了快速、准确地检测出生鲜牛乳中是否含有三聚氰胺,以保证生鲜牛乳及其产品的卫生安全。[方法]采用酶联免疫法（SNAP法）检测生鲜牛乳中三聚氰胺的含量,对新疆巴音郭楞蒙古自治州辖区内的17个奶站的奶缸、运输车辆进行生鲜乳样品抽样检测,共抽样60份。f结果]对60份生鲜乳样品进行三聚氰胺含量检测,检测结果全部为阴性。[结论]对新疆巴音郭楞蒙古自治州辖区内的17个奶站的奶缸、运输车辆所抽取的60份生鲜牛乳进行了三聚氰胺含量检测,60份生鲜牛乳样品检测结果全部为阴性,检测样品60份,合格数60份,合格率为100％。 相似文献
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牛乳中三聚氰胺和β-内酰胺酶快速检测方法的评估——双流向酶联免疫法 总被引：3，自引：0，他引：3
本试验用加标的方法制备合不同浓度三聚氰胺和β-内酰胺酶的还原乳,分别应用气相色谱-质谱/质谱法和SNAP双向酶联免疫法、SNAP双向酶联免疫间接法检测加标试样,评估SNAP双流向酶联免疫法检测牛乳中非法添加的三聚氰胺和β-内酰胺酶的精确性和灵敏度.结果表明,SNAP双流向酶联免疫法对三聚氰胺加标试样的检测限可以达到0.3ppm.最低检出限为0.1ppm,且还原乳中三聚氰胺浓度与SNAP读数仪读数呈正相关.SNAP双向酶联免疫间接检测法对β-内酰胺酶加标试样的检出限可以达到0.0005IU/mL和0.001IU/mL.SNAP流向酶联免疫法可以作为检测牛乳中非法添加的三聚氰胺和β-内酰胺酶的快速筛选方法,有较高的精确度和灵敏度,适用于奶户、奶站、乳品厂和实验室的快速筛选检测. 相似文献
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本研究运用化学计量学方法编写计算机程序并以此建立模型,通过比较不同的光谱预处理方法和建模方法,确定了最佳的近红外判别模型,建立了基于近红外技术的掺假生鲜乳识别平台,并进行了试验验证。2010年1～7月及2011年7月,采集上海市及周边地区奶牛场的生鲜乳样品287份,配制掺水量为1%～20%,含三聚氰胺、尿素或硝酸铵,但含氮量达标的掺假生鲜乳样品526份,所有样品经近红外光谱扫描后,采用留四分之一法进行建模和预测,统计准确率。结果表明,掺假生鲜乳识别平台可以较好地识别掺假生鲜乳。 相似文献
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Detection of capsular polysaccharide in milk of cows with natural intramammary infection caused by Staphylococcus aureus 总被引：1，自引：0，他引：1
Detection of capsular polysaccharide (CP) in milk of cows with natural intramammary infection caused by Staphylococcus aureus was attempted. Five quarters of 5 cows harboring S aureus strains that produce type-8 CP were selected. Using an ELISA with a monoclonal antibody, type-8 CP was not detected in extracts prepared from fresh milk collected aseptically. By contrast, CP was easily detectable after incubation of infected milk at 38 C for 20 hours. Quantitation of CP in extracts from incubated milk samples by use of ELISA indicated a great variation of CP expression by strains. Although an incubation step was necessary to detect CP, results of the study indicate that CP may be expressed in vivo during intramammary infection caused by S aureus. 相似文献
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为了解西安市售消毒乳制品中抗菌药残留情况,以农业部2001年发布的无公害食品行业标准中牛乳不得检出抗菌药为判定标准,随机采购32份消毒牛奶,进行抗菌药残留检测。TTC法检测显示,抗菌药物残留阳性数为5份,阳性率为15.63%;ELISA法检测显示,磺胺二甲基嘧啶未检出,四环素类残留阳性数为3份,阳性率为9.38%,链霉素残留阳性数为7份,阳性率为21.88%。市售不同包装消毒牛奶中除新鲜屋包装外,其余均有抗菌药残留。进口消毒奶粉未检出抗菌药,检测国产消毒牛奶32份与国产奶粉20份,共检出阳性10份与11份,阳性率分别为31.25%和55%。通过检测可看出,奶粉中抗菌药残留阳性率显著比消毒牛奶高;包装精良的高档牛奶中抗菌药残留阳性率比普通包装牛奶低;进口消毒奶粉未检出抗菌药。检测结果显示抗菌药残留情况仍不容乐观。 相似文献
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van Weering H van Schaik G van der Meulen A Waal M Franken P van Maanen K 《Veterinary microbiology》2007,125(1-2):49-58
The objective of the study was to determine the diagnostic performance of the Pourquier ELISA for detection of antibodies against Mycobacterium avium subsp. paratuberculosis (Map) in individual milk samples and in bulk milk samples. For individual milk samples the specificity of the Pourquier ELISA was estimated by testing a panel of individual milk samples from certified Map-free herds. The relative sensitivity of the assay in individual milk samples and agreement of the results with those of serum samples was estimated by testing panels of paired serum-milk samples from seropositive cattle, whole-herd investigations, and moderate or heavy shedders. The specificity of the ELISA for individual milk samples was still 99.8% at a cut-off of 20% sample to positive (S/P) value, clearly lower than the cut-off defined by the manufacturer (30% S/P). The relative sensitivity for individual milk samples as compared with positive serum samples was 87% for a cut-off of 20% S/P, and 80% for a cut-off of 30% S/P. The sensitivity of this ELISA for detection of high shedders was >90% both for individual milk and serum samples, also agreement was very good (kappa=0.91 for all paired samples). The specificity of the Pourquier ELISA in bulk milk samples was investigated by testing bulk milk samples from certified Map-free herds. Feasibility of bulk milk testing was investigated by titrating ELISA positive individual milk samples in negative milk. In addition, 383 bulk milk samples from herds with a known within-herd seroprevalence were tested. The specificity of the ELISA for bulk milk samples was 100% at a cut-off of 12.5% S/P. At the cut-off recommended by the manufacturer (30% S/P) performance of the bulk milk ELISA related to herd status (> or =2 seropositive cows) was rather poor, corresponding with a sensitivity of 24% and a specificity of 99% relative to serology. However, at the revised cut-off for bulk milk of 12.5% S/P and a within-herd seroprevalence of > or =3%, sensitivity and specificity relative to serology were 85% and 96%, respectively. Given the current herd-level seroprevalence in The Netherlands, these test characteristics corresponded with positive and negative predictive values for bulk milk of 67% and 94%, respectively. In conclusion, the diagnostic performance of the Pourquier ELISA for individual milk samples creates opportunities for a cheaper and more feasible testing scheme, while the diagnostic performance for bulk milk samples warrants further consideration. 相似文献
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宁夏地区奶牛不同饲养模式生鲜乳成分及收购状况调查 总被引：1，自引：0，他引：1
为了解宁夏不同饲养模式生鲜乳成分及收购状况,研究针对宁夏荷斯坦奶牛养殖的3种饲养模式(分别是规模场、小区、散户养殖)在2008—2009年间的饲养状况、生鲜乳成分、收购情况、日粮饲喂状况等进行了调查、统计、分析和研究。结果表明:规模场、小区、散户养殖生鲜乳的乳蛋白率分别为3.073 7%、3.037 5%、2.858 3%;乳脂率为3.641 2%、3.568 8%、3.435 0%;乳糖率分别为4.796 3%、4.686 3%、4.715 0%;非脂乳固体分别为8.505 0%、8.645 0%、8.161 7%;生鲜乳的价格分别为2.943 7,2.395 0,2.366 7元/kg。规模养殖场生鲜乳的乳蛋白率、非脂乳固体均极显著高于散户(P<0.01),乳脂率显著高于散户(P<0.05),生鲜乳价格显著高于小区、散户养殖(P<0.05)。 相似文献
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Böttcher J Meier N Stengel KH Grummer B Truyen U 《Berliner und Münchener tier?rztliche Wochenschrift》2003,116(5-6):244-251
A commercial ELISA detecting antibodies against bovine viral diarrhoea Virus (BVDV) was analysed for its applicability for bulk-milk screening. Detection limits were analysed using native and concentrated milk samples (milk treated with rennet and ammonium sulfate precipitated) from 10 cows whose sera showed different reactivity levels in the ELISA and from two cows which gave birth to persistently infected calves during the last year. Further this and a second commercial ELISA were used to screen 591 randomly selected bulk-milk samples. To clarify discrepancies thirty-nine herds were included in a follow-up study. A second bulk-milk sample and serum samples from 10 young cattle of 6 to 28 month of age per herd were analysed for antibodies against BVDV. The results of this second testing and the detection of viremic animals in 4 herds confirmed the results from initial bulk-milk testing with both tests. The analysed test is suitable for bulk-milk testing although its application is limited by vaccination. 相似文献
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Bo-Young Jeon Seung-Cheol Kim Sungmo Je Jeongyeon Kwak Jang-Eun Cho Jong-Tae Woo Sangkyo Seo Hang-Sub Shim Byoung-Ok Park Sung-Sik Lee Sang-Nae Cho 《Research in veterinary science》2010,88(3):390-393
An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of Mycobacterium bovis was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (R2 = 0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8% and 81.8%, respectively, and the specificities were 97.7% and 100%, respectively.In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5% (42/931) and the tuberculin-positive rate was 2.8% (26/931). Individual milk samples (n = 253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5% (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1% (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows. 相似文献
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Enzyme-linked immunosorbent assay for detecting antibodies to Brucella abortus in bovine milk and serum 总被引：1，自引：0，他引：1
F C Heck J D Williams J Pruett R Sanders D L Zink 《American journal of veterinary research》1980,41(12):2082-2084
An enzyme-linked immunosorbent assay (ELISA) method was evaluated for the detection of antibodies to Brucella abortus in cows milk. Milk samples from seropositive or -negative cows were sed to determine the distribution of absorbance values to classify milk as ELISA positive or ELISA negative. Brucella abortus was isolated from milk samples from 10 (45%) of the 22 cows whose milk and serum were ELISA positive. The ELISA was evaluated and determined to be an appropriate method for detecting antibodies to B abortus in bovine milk. 相似文献