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1.
急性拥挤胁迫对草鱼肌肉泛素-蛋白酶体系统活性的影响 总被引：1，自引：1，他引：0
泛素-蛋白酶体系统(theubiquitin-proteasomesystem,UPS)是细胞内调节蛋白质代谢的重要系统。在水温(17.0±0.5)℃下,将草鱼(Ctenopharyngodon idellus)暴露于100 kg/m2的养殖密度条件进行急性拥挤胁迫。在胁迫0 h、1 h、6 h、12 h、24 h和48 h以及取消胁迫后(养殖密度10 kg/m2)的6 h和168 h采集血清和背部肌肉样品,检测血清皮质醇水平、肌肉质构特性以及nrf2、hsp70和UPS相关基因表达,并定量测定了肌肉组织泛素化蛋白水平。结果表明,拥挤胁迫导致血清皮质醇含量显著上升,肌肉硬度和凝聚性显著性下降,以上各指标在取消胁迫后168 h恢复到对照水平,弹性、胶黏性、咀嚼性和回复性在恢复168h时反而高于对照组水平; hsp70和nrf2的表达量在胁迫48 h时显著高于对照组,后期恢复到对照水平;肌肉ub、psma2、psmc1、mafbx和chip的mRNA表达量在胁迫过程中显著性上升,胁迫后恢复168 h,还是表现出较高的表达量;处理组泛素化蛋白水平在胁迫6 h和12 h时显著升高,在恢复168 h时显著降低。研究表明,急性拥挤胁迫使草鱼产生明显的应激反应,对肌肉质构特性产生了显著性影响,同时提高了肌肉UPS的活性。经过168h的恢复,鱼体逐渐恢复到正常生理水平,但机体UPS活性仍呈现出被激活状态。急性拥挤胁迫对草鱼生理功能和肌肉质构特性的有害影响是可逆的,建议恢复时间大于168 h以帮助鱼体重新建立UPS的稳态。 相似文献
2.
为探讨香菇多糖(Lentinan,LNT)对黄颡鱼(Pelteobagrus fulvidraco)抗氧化及非特异性免疫指标的影响,用添加不同水平香菇多糖的饲料进行投喂试验.将540尾鱼随机分成6组,每组3个平行,每个平行30尾鱼,分别投喂添加0、300、600、900、1200、1500 mg· kg-1香菇多糖的饲料,饲养期为8周.试验结束后测定黄颡鱼体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LSZ)活力,检测丙二醛(MDA)、一氧化氮(N0)的含量、吞噬细胞的吞噬百分比、吞噬指数以及攻毒后的死亡率与免疫保护率等指标.结果表明:与对照组相比,各试验组黄颡鱼体内SOD、CAT的活力提高,MDA的含量降低(P＜0.05);各试验组鱼体各组织的NO含量均有提高,但与多糖浓度成负相关;添加香菇多糖能显著提高鱼体内溶菌酶的活性、鱼体吞噬细胞的吞噬百分比和吞噬指数(P＜0.01),并可降低攻毒后鱼体的死亡率和提高免疫保护率.由此可见,在饲料中添加香菇多糖对提高黄颡鱼体内抗氧化及特异性免疫能力具有良好的效果. 相似文献
3.
开展了饲料中小球藻添加量对杂交黄颡鱼“黄优1号”生长性能和抗氧化能力影响试验。共设置5组,分别为1个对照组(G0)和4个处理组(G1—G4)。其小球藻的添加量分别为0.00%(G0),2.50%(G1),5.00%(G2),7.50%(G3)和10.00%(G4)。于养殖的第28和56天,测定杂交黄颡鱼“黄优1号”鱼体生长指标和抗氧化能力指标。结果表明,随着饲料中小球藻含量的增加,杂交黄颡鱼“黄优1号”的体质量增加率和特定体质量增加率呈上升趋势,并显著高于对照组(P<0.05),饵料系数呈下降趋势,肝体指数较对照组无显著差异(P>0.05)。抗氧化能力方面,饲料中添加小球藻可以显著提高杂交黄颡鱼“黄优1号”肝脏和血清中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性,能降低鱼体内丙二醛含量。指出,饲料中添加小球藻不仅可以促进杂交黄颡鱼“黄优1号”的生长,并提高其抗氧化能力。 相似文献
4.
《中国渔业质量与标准》2016,(1)
以肌肉腐烂的黄颡鱼(Pelteobagrusfulvidraco)为研究对象,从发病鱼体的肝、脾中分离出了一株致病菌(命名为JY-03),经革兰氏染色、显微观察、API生理生化实验24~48 h,最终鉴定该病原菌为温和气单胞菌(Aeromonassobria)。通过感染实验再次证实黄颡鱼肌肉腐烂的致病菌为温和气单胞菌。对发病池塘水质分析表明,养殖水体中存在大量微囊藻,加速了水体中致病菌繁殖速度,致使发病池塘中黄颡鱼暴发肌肉腐烂病。药物的治疗实验表明,10%诺氟沙星与10%恩诺沙星分别与饲料按照1∶1 000(m/m)比例拌料,每日按照鱼体重1%投喂饲料1次,两种药物对该病均有明显治疗效果,鱼体皮肤溃烂征状逐步消失,死亡数量显著下降,疫病得到有效控制,用药2周后基本治愈;同等的用药剂量下,恩诺沙星治疗效果相对更好。本研究提供了黄颡养殖生产过程中肌肉腐烂病发病原因的鉴定方法和治疗方案,对黄颡鱼的健康养殖及相关研究均具有一定的参考意义。 相似文献
5.
黄颡鱼饲料脂肪的最适含量 总被引：5，自引：0，他引：5
以鱼油/豆油(1∶1,W/W)为脂肪源,配制脂肪含量为5.21%、7.23%、9.27%、11.31%、13.33%、15.37%的半纯化饲料,投喂体重约8g的黄颡鱼40d,用于探讨饲料脂肪水平对黄颡鱼生长及饲料利用率的影响。试验结果表明当脂肪含量<11.31%时,鱼体的相对增重率、饲料转化率、蛋白质效率与脂肪含量呈正相关,当脂肪含量>11.31%时,鱼体的相对增重率、饲料转化率、蛋白质效率与脂肪含量呈负相关。肝体比和肌肉渗出损失随着脂肪添加量的增加而持续上升。脂肪水平对以上各项指标有显著性影响(P<0.05)。综合各项指标,可以确定黄颡鱼饲料脂肪的最适含量为11.31%。 相似文献
6.
为研究宰杀后的黄颡鱼(Pelteobagrus fulvidraco)在不同环境温度下冰藏过程的品质变化和保鲜时间,测定了不同环境温度冰藏黄颡鱼肌肉的质构指标、pH、硫代巴比妥酸(TBA)、高铁肌红蛋白(MetMb)、挥发性盐基氮(TVB-N)、营养成分(水分、粗蛋白、粗脂肪、粗灰分)的含量,并通过16S rRNA高通量测序分析了黄颡鱼肌肉微生物菌群的变化。结果显示：15、27、33℃温度下冰藏黄颡鱼肌肉各质构指标均随时间延长而下降。各温度组TBA、TVB-N和MetMb含量随时间延长呈上升趋势,三组的TVB-N和MetMb含量在12 h冰藏期间均没有超过国家标准限值,15℃组TVB-N含量始终处于一级鲜度标准。各温度组肌肉营养成分中粗脂肪、粗灰分和水分含量随冰藏时间延长而有所下降。微生物组分析表明在冰藏期间,三个温度组的黄颡鱼肌肉均未产生有害病原菌微生物。研究表明,宰杀后的黄颡鱼33℃时冰藏保鲜时长为4 h, 27℃时冰藏保鲜时长为6 h, 15℃时冰藏保鲜时长可达12 h。 相似文献
7.
MS-222对黄颡鱼麻醉效果的研究 总被引：2，自引：0，他引：2
为研究MS-222质量浓度和药浴时间对黄颡鱼麻醉效果的影响,采用静水式生物测试法进行了黄颡鱼麻醉效果试验。在水温(10±0. 5)℃下,研究了黄颡鱼在短时间(30 min)麻醉药浴和长时间(24 h)镇静药浴下各麻醉阶段的行为特征、诱导时间和呼吸频率,以及在复苏阶段的行为特征、复苏时间和复苏后存活率等指标。结果显示,在短时间麻醉药浴试验中,MS-222质量浓度为60 mg/L的处理组,黄颡鱼5 min 30 s即可进入麻醉期,在空气中暴露10 min依然保持麻醉状态,放入清水中4 min 5 s即可复苏。这一处理方法适用于黄颡鱼的短时间转运等操作。MS-222质量浓度为20 mg/L的处理组,黄颡鱼可长时间(24 h)处于镇静状态,停止游动,鱼体保持平衡,且触觉丧失,存活率达到100%。这种处理方法适用于黄颡鱼的长时间运输。 相似文献
8.
慢性氨氮暴露诱发黄颡鱼幼鱼谷氨酰胺积累、氧化损伤及免疫抑制的研究 总被引：2，自引：2，他引：0
为了研究慢性氨氮胁迫对黄颡鱼幼鱼生长性能、大脑谷氨酰胺积累、肝脏抗氧化酶活性、非特异性免疫应答及抗嗜水气单胞菌感染能力的影响,实验挑选初始体质量为(1.94±0.05)g的健康黄颡鱼幼鱼180尾,开展为期56 d的慢性氨氮胁迫实验.结果表明,实验鱼的终末体质量、增重及饲料效率,氨氮组与对照组无显著性差异,但肝体比氨氮组显著高于对照组;氨氮组实验鱼大脑中氨氮和谷氨酰胺含量显著高于对照组,但谷氨酸含量各组间无显著性差异;氨氮组实验鱼肝脏中超氧化物歧化酶、过氧化物酶及谷胱甘肽过氧化物酶活性显著低于对照组,但硫代巴比妥酸反应物含量氨氮组显著高于对照组;氨氮组实验鱼肝脏中溶菌酶活性、头肾巨噬细胞吞噬指数和呼吸爆发显著低于对照组;感染嗜水气单胞菌14d后,氨氮组和对照组实验鱼累计死亡率无显著性差异.研究表明,黄颡鱼幼鱼遭受亚致死浓度的慢性氨氮胁迫,能够导致大脑中谷氨酰胺含量升高;氨氮组黄颡鱼肝脏中硫代巴比妥酸反应物的过度积累表明,应激产生的大量自由基并不能被机体自身的抗氧化酶体系完全清除;亚致死浓度的慢性氨氮胁迫会对黄颡鱼幼鱼的免疫应答体系造成抑制. 相似文献
9.
为探究感染累枝虫(Epistylis sp.)的黄颡鱼(Pelteobagrus fulvidraco)病愈过程中血液生化指标的变化规律,测定了患病鱼和经药物治疗痊愈及健康的黄颡鱼的血清皮质醇、葡萄糖、乳酸(LD)含量以及碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、谷草转氨酶(AST)、谷丙转氨酶(ALT)的活性。结果显示,患病组黄颡鱼血清皮质醇、血糖含量和AST活性均显著高于痊愈组(P0.05),血清ALT活性与痊愈组无显著差异(P0.05)。与患病组相比,痊愈组黄颡鱼血清LD含量,LDH和ALP活性均显著升高(P0.05)。与健康组相比,痊愈黄颡鱼血清皮质醇含量和ALT活性无显著差异(P0.05),而葡萄糖、LD含量,LDH、AKP和AST活性则差异显著(P0.05)。结果表明,累枝虫的寄生引起了黄颡鱼显著的应激反应,使鱼体非特异性免疫能力下降;经治疗痊愈后的黄颡鱼的非特异性免疫力可恢复正常,鱼体应激反应消失并表现出正常的游泳行为。 相似文献
10.
11.
为了了解池塘和水库网箱两种养殖模式下斑点叉尾鮰（Ictalurus punctatus）肌肉营养成分和品质特性,利用生化分析、物性分析方法和电感耦合等离子体原子发射光谱法分别对斑点叉尾鮰肌肉的常规营养成分、系水力、质构特性及肌肉矿物元素含量进行了测定。结果显示,池塘和水库网箱养殖的斑点叉尾鮰含肉率都在65%以上;池塘组的贮存损失和失水率显著低于水库网箱养殖组,而冷冻渗出率显著高于水库网箱养殖组;水库网箱养殖组的粗蛋白、粗灰分含量均显著高于池塘组,而粗脂肪含量显著低于池塘组;两组中矿物元素含量都比较丰富,水库网箱养殖组肌肉中钾、磷、钙、镁、锌、铁六种元素的含量均显著高于池塘组;池塘组肌肉的凝聚性和回复性显著高于水库网箱养殖组,而肌肉硬度、胶黏性和咀嚼性显著低于水库网箱养殖组。研究表明,池塘养殖的斑点叉尾鮰肌肉具有较好的系水力,而水库网箱养殖的斑点叉尾鮰肌肉具有高蛋白低脂肪、矿物元素更丰富、肉质硬度大的特点。 相似文献
12.
池养黄颡鱼运输应激后在实验循环系统中的恢复和适应过程 总被引：3，自引：2，他引：3
本文将土池养殖的黄颡鱼成鱼运往室内实验循环系统驯养，并对鱼的摄食及鱼对日常管理活动的反应、血液皮质醇和血糖、外周血红细胞数量、外周血白细胞百分比和吞噬功能进行了35 d的观察或监测。运输后的黄颡鱼在最初4～7 d内，明显的行为变化是停止摄食。所有的鱼从第8天开始恢复了摄食，从第15天开始适应了日常管理活动。血液皮质醇和血糖浓度在刚刚运抵实验循环系统（0 d）及驯养1、2、3 d的鱼体内显著升高，至第7天已显著下降，并在第21、28、35天稳定在低水平上。外周血红细胞数量没有显著变化。与第21、28、35天外周血白细胞的各参数值相比，淋巴细胞百分比在第3、7天显著减少，嗜中粒细胞百分比在0、1、2、3天显著升高，吞噬细胞吞噬百分比和吞噬指数在第7、14天显著降低。以上结果显示（1）运输使黄颡鱼成鱼产生典型的应激反应，（2）运输应激导致黄颡鱼成鱼外周血淋巴细胞和嗜中粒细胞百分比的显著改变和外周血白细胞吞噬功能的抑制，（3）运输应激后的池养黄颡鱼成鱼在本文使用的实验循环系统中充分恢复和驯化所需的最少时间是3周。 相似文献
13.
Remy Manuel Jeroen Boerrigter Jonathan Roques Jan van der Heul Ruud van den Bos Gert Flik Hans van de Vis 《Fish physiology and biochemistry》2014,40(1):33-44
Of the many stressors in aquaculture, transportation of fish has remained poorly studied. The objective of this study was therefore to assess the effects of a (simulated) commercial transportation on stress physiology of market-size African catfish (Clarias gariepinus). Catfish weighing approximately 1.25 kg were returned to the farm after 3 h of truck-transportation, and stress-related parameters were measured for up to 72 h following return. Recovery from transportation was assessed through blood samples measuring plasma cortisol, glucose and non-esterified fatty acids (NEFA) and gill histology. Also, the number of skin lesions was compared before and after transport. Pre-transport handling and sorting elevated plasma cortisol levels compared to unhandled animals (before fasting). Plasma cortisol levels were further increased due to transportation. In control fish, plasma cortisol levels returned to baseline values within 6 h, whereas it took 48 h to reach baseline values in transported catfish. Plasma glucose and NEFA levels remained stable and were similar across all groups. Transported catfish did not, on average, have more skin lesions than the handling group, but the number of skin lesions had increased compared to unhandled animals. The macroscopic condition of the gills was similar in control, transported and unhandled catfish; however, light microscopy and immunohistochemistry revealed atypical morphology and chloride cell migration normally associated with adverse water conditions. From our data, we conclude that transportation may be considered a strong stressor to catfish that may add to other stressors and thus inflict upon the welfare of the fish. 相似文献
14.
为探究柱形病对黄颡鱼(Pelteobagrus fulvidraco)生理机能的影响,随机选取患病和健康黄颡鱼各10尾,采集其血液,静置后离心分离血清以测定血液生化指标如皮质醇、乳酸(LD)含量,乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)活性,葡萄糖、白蛋白(ALB)、总蛋白(TP)、尿素氮(BUN)、总胆固醇(TCHO)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)含量,谷草转氨酶(AST)、谷丙转氨酶(ALT)活性。结果显示:患病黄颡鱼血清皮质醇浓度和LDH、AST活性均极显著高于健康鱼,LD含量和ALT活性显著高于健康鱼。与健康鱼相比,患病黄颡鱼血清葡萄糖含量极显著降低,而HDLC、LDLC、TCHO、ALB、TP、BUN浓度和ALP活性无显著差异。健康鱼肝细胞呈近圆形,形状较规则,细胞界线清晰,细胞核多位于细胞中央,呈圆形或椭圆形,肝细胞以中央静脉为中心形成放射状排列。患病鱼肝细胞出现局部坏死、空泡化,形成坏死灶,伴有大量中性粒细胞、淋巴细胞浸润。结果表明:柱状黄杆菌感染可引起黄颡鱼鱼体显著的应激反应,并引起鱼体肝脏损伤。 相似文献
15.
The present study evaluated the physiological responses of matrinxã, Brycon cephalus (Günther) submitted to transport stress under the influence of sodium chloride. Different salt concentrations (0.0%, 0.1%, 0.3% and 0.6%) were added to four 200-L plastic tanks. Each tank was stocked with 30 fish (mean weight 1.0 ± 0.2 kg) and transported for 4 h. Blood was sampled prior to transport and immediately after and 24 and 96 h after transport. Plasma cortisol and glucose and serum sodium and potassium, plasma chloride and ammonia were analysed. Changes in plasma cortisol were observed immediately after transportation, except in fish transported in 0.3% and 0.6% salt. Twenty-four hours later, this hormone had returned to its initial level in all fish. Blood glucose was not changed in fish treated with 0.6% salt immediately after transport, and returned to the initial level within 96 h after the other treatments. All treatments resulted in lower levels of plasma chloride after transport, except for fish treated with 0.6% salt, with fish treated with 0.0% and 0.3% salt recovering 24 h later. Serum sodium decreased immediately after transport only in the control fish, returning to the initial level 24 h later. The results indicate that treatment with 0.6% NaCl reduces most of the physiological responses of matrinxã to the stress of transport. 相似文献
16.
为探究大菱鲆分别在有水及无水条件下运输过程中生理生化指标及肌肉品质的变化,为大菱鲆的保活运输提供参考。将大菱鲆进行暂养、降温、充氧包装后进行模拟运输,并在运输不同时间段取样,检测大菱鲆生理生化指标及肌肉指标。结果显示,模拟运输18 h后,有水运输组和无水运输组中的大菱鲆存活率仍为100%。大菱鲆肌肉中水分含量、粗脂肪、pH、糖原、三磷酸腺苷(ATP)和腺苷酸(AMP)含量显著降低,乳酸和肌苷酸(IMP)含量升高。其中,有水运输组中的大菱鲆AMP含量降低了38.65%,IMP含量增加了29.1%,无水运输组中的大菱鲆AMP含量降低了12.83%,IMP含量增加了28.47%。大菱鲆血液生化指标中,葡萄糖、尿素氮和皮质醇含量显著升高。运输过程后,无水运输组中大菱鲆生理应激及能量代谢指标的变化幅度均小于有水运输处理组中的大菱鲆。此外,低温无水运输组大菱鲆肌肉中呈味核苷酸IMP和AMP含量显著高于有水运输组,表明低温无水运输增加了大菱鲆呈鲜味物质的含量,提高了鱼肉风味。研究表明,低温无水保活技术在活鱼运输中具有广泛的应用前景。 相似文献
17.
氧化鱼油对黄颡鱼幼鱼肠道健康的影响及精氨酸的干预作用 总被引：1，自引：1，他引：0
为研究饲料中添加氧化鱼油对黄颡鱼幼鱼肠道免疫、抗氧化和其他功能指标、组织结构的影响及添加精氨酸对其的干预作用,采用2×3设计方式,在饲料中分别添加新鲜鱼油(FF)∶氧化鱼油(OF)按照(m/m)2.5∶0、1.5∶1.0和0.5∶2.0的比例配制3种基础饲料(FF,OF1,OF2),在基础饲料中分别添加0.48%精氨酸(Arg)盐酸盐配制3种精氨酸饲料(FFA,OFA1,OFA2),选取初始体质量为(4.41±0.05)g的黄颡鱼600尾,随机分为6组,每组4个重复,分别投喂6种实验饲料,饲养56 d。结果显示,在OF2组中,黄颡鱼肠道酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性和白细胞介素-6(IL-6)含量显著升高;与OF2组相比,OFA2组AKP活性和IL-6含量均显著下降。在FF、OF1、OF2三组中,肠道超氧化物歧化酶(SOD)活性呈下降趋势,谷胱甘肽过氧化物酶(GSH-PX)和过氧化氢酶(CAT)活性呈现上升趋势,总抗氧化能力(T-AOC)呈现先下降后上升的趋势,但差异均不显著;在OF2组中,丙二醛(MDA)含量显著升高;添加精氨酸后,除SOD活性有显著升高外,其他抗氧化指标的组间均无显著性差异。双因素方差分析显示,精氨酸对黄颡鱼肠道CAT活性的影响达到显著水平,饲料中氧化鱼油和精氨酸对黄颡鱼肠道GSH-PX活性的影响存在交互作用。在FF、OF1、OF2三组中,肠道二胺氧化酶(DAO)活性呈现下降趋势,一氧化氮合酶(i NOS)活性呈现上升趋势,但差异均不显著;与OF2组相比,OFA2组的DAO和i NOS活性分别显著升高和下降;双因素方差分析显示,精氨酸(Arg)对黄颡鱼肠道DAO、i NOS活性的影响分别达到显著水平。在OF1组中,肠道皱壁高度、肌层厚度和杯状细胞数量均明显升高;与OF2组相比,OFA2组皱壁高度和杯状细胞数量明显升高;与FF组相比,FFA组肌层厚度明显增加。双因素方差分析显示,氧化鱼油对黄颡鱼肠道皱壁高度的影响达到显著水平。研究表明,在饲料中添加一定水平的氧化鱼油会抑制黄颡鱼幼鱼肠道免疫及抗氧化指标,损伤肠道组织结构,但添加一定量的精氨酸可以缓解氧化鱼油对黄颡鱼幼鱼肠道免疫、抗氧化和组织结构的抑制作用。 相似文献
18.
Effects of feeding frequency on the enzymes and genes involved in oxidative stress in juvenile yellow catfish Pelteobagrus fulvidraco (Richardson) exposed to ammonia 
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下载免费PDF全文 Xiwei Fan Ming Li Lixia Yuan Hang Lai Meize Song Rixin Wang Rongquan Zheng 《Aquaculture Research》2017,48(12):5874-5882
Optimal feeding strategies improve fish growth and health but may be affected by ammonia stress in closed rearing systems such as tanks or ponds. This study aimed to evaluate the effects of feeding frequency and ammonia levels in rearing water on the enzymes and genes involved in oxidative stress of yellow catfish. Experiment (ammonia exposure) and control groups were randomly assigned to one of three feeding frequencies (1, 2 and 4 times daily) for 8 weeks. Weight gain increased as feeding frequency increased from 1 to 4 times daily, but feed conversion ratio values decreased. The highest survival in ammonia group was found when fish was fed 2 times daily. Glutathione peroxidase activity and total antioxidant capacity in liver and brain of fish exposed to ammonia increased as feeding frequency increased from 1 to 4 times daily. Liver malondialdehyde content in control group decreased as feeding frequency increased from 1 to 4 times daily. The lowest liver malondialdehyde content in ammonia group was observed when fish was fed 2 times daily. Liver mRNA expression of superoxide dismutase and catalase in control group increased as feeding frequency increased from 1 to 4 times daily, but the highest superoxide dismutase and catalase expression in ammonia group were observed when fish were fed 2 times daily. This study indicates that higher feeding frequency of yellow catfish exposed to ammonia could result in oxidative stress and poor survival. The optimal feeding frequency of yellow catfish exposed to ammonia is 2 times daily. 相似文献
19.
Carine de Freitas Souza Matheus Dellaméa Baldissera Adriane Erbice Bianchini Elisia Gomes da Silva Rosa Helena Veraz Mourão Lenise Vargas Flores da Silva Denise Schmidt Berta Maria Heinzmann Bernardo Baldisserotto 《Fish physiology and biochemistry》2018,44(1):21-34
The viability using Lippia alba essential oil as an anesthetic for fish was studied, particularly with respect to physiological effects during recovery. Anesthesia of silver catfish (Rhamdia quelen) using 100 and 300 μL L?1 of two different chemotypes of L. alba essential oil (citral EO-C and linalool EO-L) prevented the increase of plasma cortisol levels caused by handling, but did not avoid alterations in energetic metabolism. Silver catfish did not have increased the levels of thiobarbituric acid reactive species in the kidney and liver during recovery after anesthesia with either EO, avoiding lipid damage. On the other hand, fish anesthetized with EO-C showed higher protein carbonylation levels, superoxide dismutase, catalase, and glutathione S-transferase activities and non-protein thiol group levels in both tissues compared to controls. Our results suggest that both oils show antioxidant capacity, but anesthesia with EO-L does not cause damage to lipids or proteins, only temporary changes, typical of physiological adjustments during recovery from anesthesia. Therefore, EO-L is an effective anesthetic for silver catfish with fewer side effects than EO-C. 相似文献
20.
Kenneth B. Davis Bill A. Simco Meng Li Edwin Robinson 《Journal of the World Aquaculture Society》1998,29(3):319-324
Abstract— The influence of reduced amounts of supplemental vitamins on the stress response of channel catfish lctalurus punctatus was determined. Fish were fed diets with reduced levels of supplemental vitamin C, niacin, riboflavin, pantothenic acid, pyridoxine or thiamine, or with reduced amounts of the total vitamin supplement typically added to catfish feed. Fish were confined for 6 h and blood samples taken at 0, 1 and 6 h of confinement and 12 h after release. Plasma chloride and osmotic pressure were consistently stable throughout the experiment and were not influenced by the diet or the stress imposed. Cortisol concentrations were elevated at 1 and 6 h of confinement but recovered to preconfinement concentrations 12 h after release. The cortisol response varied in some of the groups; however, no consistent effects due to the vitamin concentrations were observed. The lack of supplemental vitamins in the food did not impair the ability of the fish to mount or maintain cortisol secretion. The feed ingredients may contain enough vitamins to support cortisol synthesis or the fish may be acquiring sufficient additional vitamins from the pond environment or from intestinal bacteria. 相似文献