共查询到18条相似文献，搜索用时 15 毫秒
1.
用识别5、6碱基序列的17种限制性内切酶,即BamHⅠ、BglⅠ、BglⅡ、DraⅠ、EcoRⅠ、EcoRⅤ、HindⅢ、KpnⅠ、MluⅠ、PstⅠ、PvuⅡ、SalⅠ、ScaⅠ、SmaⅠ、StyⅠ、XbaⅠ和XhoⅠ,对南海海域石斑鱼属(EpinephelusBloch)野生蜂巢石斑鱼(E.merra)、鲑点石斑鱼(E.fario)、青石斑鱼(E.awoara)、赤点石斑鱼(E.akaara)和七带石斑鱼(E.septem-fasciatus)的线粒体DNA(mtDNA)进行RFLP分析。测算出蜂巢石斑鱼、鲑点石斑鱼、青石斑鱼、赤点石斑鱼和七带石斑鱼的mtDNA分子大小分别为(18.52±0.21)kb、(18.44±0.21)kb(、18.56±0.18)kb、(18.57±0.19)kb和(18.36±0.11)kb。通过单、双酶切分析,分别构建了蜂巢石斑鱼10种酶共20个酶切位点、鲑点石斑鱼9种酶共17个酶切位点、青石斑鱼8种酶共16个酶切位点、赤点石斑鱼7种酶共12个酶切位点和七带石斑鱼7种酶共13个酶切位点的酶切物理图谱。BglⅡ和XhoⅠ两种内切酶的酶切谱带可作为鉴别这5种石斑鱼的RFLP标记。 相似文献
2.
四种石斑鱼氨基酸组成的研究 总被引：30，自引：1，他引：30
测定分析了采用于我国南海不同海域的鲑点石斑鱼、蜂巢石斑鱼、黑边石斑鱼和巨石斑鱼６４个标本的１８种氨基酸含量。结果表明，石斑鱼肌肉中氨基酸总含量、必需氨基酸含量和鲜味氨基酸含量均较高。氨基酸组成的种间和分布海域间的差异不大，而肌肉、卵巢、肝脏、骨骼、鳞片和皮肤等不同组织（器官）间的变化较大，石斑鱼氨基酸组成存在月际变化现象，且随体长的增长而变化。天然的与养殖的石斑鱼氨基酸组成也有一定差异。这４种石斑 相似文献
3.
石斑鱼类的人工繁育技术 总被引：2，自引：0，他引：2
石斑鱼（Epinephalus）属鲈形目能科,是广泛分布于印度洋和太平洋的暖水性鱼类,在中国近海有31种,其中,南海有30种,东海8～9种,黄渤海仅1种。目前我国大陆沿海养殖的种类主要有：赤点石斑鱼、青石斑鱼、点带石斑鱼、斜带石斑鱼、鲑点石斑鱼、云纹石斑鱼、鞍带石斑鱼等。 相似文献
4.
5.
5种石斑鱼遗传差异的RAPD分析 总被引：3，自引：0，他引：3
运用随机扩增多态性DNA（random amplified polymorphic DNA,RAPD）技术对鞍带石斑鱼（Epinephelus lanceolatus）、驼背鲈（Cromileptes altivelis）、棕点石斑鱼（E.fuscoguttatus）、斜带石斑鱼（E.coioides）和鲑点石斑鱼（E.fario）的遗传多样性及系统发生进行了研究。从120个RAPD随机引物中筛选出扩增效果好的引物19个,分别用于5种石斑鱼基因组DNA的扩增。结果显示,5种石斑鱼平均多态性位点比率（P）分别为64.23%,72.61%,60.34%,69.97%,73.94%;个体间平均遗传相似系数（S）分别为0.8238,0.8110,0.8345,0.8277,0.8064;平均遗传距离（D）分别为0.1762,0.1890,0.1655,0.1723,0.1936;平均Nei基因多样性指数（H）分别为0.1189,0.1364,0.1028,0.1439,0.1648;平均Shannon信息指数（Hi）分别为0.1801,0.1992,0.1530,0.2100,0.2434。5个种间的遗传距离（Dxy）在0.3964～0.6085之间。 相似文献
6.
7.
石斑鱼隶属于鮨科，绝大部分是石斑鱼属的鱼类，如赤点石斑鱼、巨石斑鱼、点带石斑鱼和鲑形石斑鱼等，但有一些其他属的鱼类习惯上也被称为石斑鱼，如被称为老鼠斑的驼背鲈。 相似文献
8.
两种杂交石斑鱼子一代杂种优势的微卫星标记分析 总被引：5，自引：3，他引：2
使用6对微卫星引物对两种杂交子一代(青龙斑和虎龙斑)及其亲本(斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼)共5个群体进行微卫星分析,计算等位基因频率、有效等位基因数、基因杂合度、多态信息含量、相似指数和Nei氏遗传距离。结果显示,在5种石斑鱼中,棕点石斑鱼的平均有效等位基因数最大(6.849 3),最小的是鞍带石斑鱼(2.608 6)。6对微卫星引物在这5个群体中具有丰富的多态性,平均多态信息含量(PIC)分别为0.666 7、0.751 3、0.441 9、0.664 0、0.542 6。在平均观测杂合度(Ho)中,虎龙斑和青龙斑的最高均为0.940 0,鞍带石斑鱼的最低为0.508 3。遗传距离和遗传相似率结果显示,青龙斑和虎龙斑均与父本鞍带石斑鱼的亲缘关系较近。杂交子一代群体的等位基因基本来自父母本群体双方,可推断杂交子一代的遗传物质来自父母双方,属两性融合生殖,是真正意义上的杂交种。杂交后代的遗传变异水平明显增强,这是杂种优势得以形成的重要遗传物质基础。 相似文献
9.
福建沿海赤点石斑鱼资源状况的分析 总被引：1，自引：0，他引：1
我国东南沿海有36种石斑鱼,而福建沿海常见的种类是赤点石斑鱼(Epinephe-lus akaara)、青石斑鱼(E.awoara)、云纹石斑鱼(E.moara)、鲑点石斑鱼(E.fario)和六带石斑鱼(E.sexfasciatus)等,其中以赤点石斑鱼(俗称红斑)的数量为最多,是一种重要的出口创汇的名贵鱼类,1984年福建省石斑鱼产量估计为400吨。 相似文献
10.
本研究利用20对石斑鱼微卫星引物对金虎斑[棕点石斑鱼(Epinephelus fuscoguttatus) (♀)´蓝身大斑石斑鱼(E. tukula) (♂)]及其父母本进行微卫星分析,从分子水平对金虎斑及其双亲的遗传多样性进行研究。结果显示,20个微卫星位点共检测到215个等位基因,平均多态信息含量(PIC)为0.638 6;棕点石斑鱼平均有效等位基因数(Ne)最多(4.367 9),金虎斑次之(3.370 6),蓝身大斑石斑鱼最少(2.412 8);蓝身大斑石斑鱼、棕点石斑鱼和金虎斑3个群体平均观测杂合度(Ho)分别为0.385 5、0.474 9和0.473 6,PIC分别为0.491 9、0.645 3和0.555 1;3种石斑鱼群体群内近交系数(Fis)、总群体近交系数(Fit)、遗传分化系数(Fst)和基因流(Nm)平均值分别为0.228 0、0.344 1、0.150 4和1.411 9,群体间分化较大;采用UPGMA法构建的聚类分析图显示,金虎斑与棕点石斑鱼先聚为一类,二者亲缘关系更近。研究表明,金虎斑存在较丰富的遗传多样性表现,在遗传学方面可进行杂交石斑鱼进一步选育及杂种优势研究。 相似文献
11.
南海主要珊瑚礁(鱼旨)科鱼类的组成与分布 总被引：2，自引：0，他引：2
1998年3~5月和1999年5~6月,采用手钓、延绳钓和刺网3种作业方式在西沙的浪花礁、华光礁、琛航岛、永兴岛、北礁和南沙的渚碧礁、南薰礁、东门礁、美济礁、赤瓜礁、永署礁、华阳礁等12座珊瑚礁水域进行了2个航次的鱼类资源调查,捕获科鱼类6属25种,渔获量计942尾256.4kg;其中在南沙调查区捕获6属22种,共641尾205.5kg,优势种依次为尾纹九棘鲈、蜂巢石斑鱼、六角石斑鱼、黑边石斑鱼;在西沙调查区捕获6属14种,共301尾50.9kg,优势种依次为尾纹九棘鲈、六角石斑鱼、黑边石斑鱼、蜂巢石斑鱼,同时分析了主要种类尾纹九棘鲈、六角石斑鱼和蜂巢石斑鱼的生物学特征。 相似文献
12.
Evaluation of genetic diversity of eight grouper species <Emphasis Type="Italic">Epinephelus</Emphasis> spp. based on microsatellite variations 总被引：1，自引：0，他引：1
Worawut Koedprang Uthairat Na-Nakorn Masamichi Nakajima Nobuhiko Taniguchi 《Fisheries Science》2007,73(2):227-236
ABSTRACT: Genetic diversity among eight species of grouper, Epinephelus bleekeri , E. coioides , E. malabaricus , E. ongus , E. akaara , E. maculatus , E. merra and E. fuscoguttatus , was studied using six microsatellite loci, Em-01 *, Em-03 *, Em-07 *, Em-08 *, Em-10 *, and CA-07 *, with the aim of exploring the feasibility of using microsatellite data for species identification. The results showed high levels of genetic differentiation among species ( F ST = 0.4403 and R ST = 0.4954). Species identification based on fixed allelic differences was possible between E. coioides , E. ongus , and E. fuscoguttatus at Em-01 * and between E. fuscoguttatus and E. ongus at Em-08 *. Private alleles were found in all species, except for E. ongus . Pairwise F ST ranged 0.238–0.578 ( P < 0.008 Bonferroni correction), and Nei's genetic distance ranged 0.433–2.710. Size homoplasy was observed at Em-03 * 157 allele, which was characterized by a T–C transition at the 119th nucleotide site of PCR products. The genetic assignment test unambiguously assigned each individual to the correct species. Thus, this test can be used for species identification of unknown individuals when the multilocus genotypes of the six microsatellite loci are available. The phylogenetic (neighbor-joining) tree, which was constructed based on the genetic distance matrix, separated the eight grouper species into two main groups. 相似文献
13.
有三种石斑鱼俗名都叫“青斑”。即：“红点青斑”Epinephelus．coioides（另一学名为E．suillus）、“黑点青斑”E.malabaricus（另一学名为E.salmonoides）及E.tauvina,即巨石斑鱼。其中最容易混淆的是“红点青斑”E.coioides和“黑点青斑”E.malabaricus。两者共同点是身上都布满斑点;都有5条深褐色的斜纹;繁殖季节、产卵习性、生长速度等大致相同。但E.malabaricus的斑点小呈黑褐色,并分布到下颌腹面,身上有白点及白斑。E.coioides的斑点大呈红褐色的,前颌腹面没有斑点,身上无白点及白斑。以往一些文献的学名及其对应的中文名称存在不少错误和混乱。本文建议：中文名称“点带石斑鱼”,“斜带石斑鱼”应该合并作为E.coioides的同种异名使用。E.malabaricus的中文名称应为“马拉巴石斑鱼”比较合适。 相似文献
14.
Ryosuke Murata Hirofumi Karimata Mohammad Ashraful Alam & Masaru Nakamura 《Aquaculture Research》2010,41(2):303-308
The Malabar grouper Epinephelus malabaricus is an important candidate species for commercial aquaculture in tropical and subtropical areas. In nature, this species requires more than 10 years to change sex from female to male and have active spermatogenic tissues in the testis. Thus, it is essential to find a means of producing sperm for seed production. This is the first report of artificial sex change in underyearling E. malabaricus . Female E. malabaricus with immature ovaries at 144 days post-hatch (DPH) were fed a diet with 17α-methyltestosterone (MT) at 50 μg g−1 diet for 6 months. Sex change occurred in most of the treated fish, which had testis with all stages of spermatogenic germ cells including spermatozoa. In contrast, most of the control fish had immature ovaries. These results, which reveal that germ cells in the underyearling grouper have the ability to produce spermatozoa in response to exogenous androgen, demonstrate that sex change can be artificially induced during ovarian development. 相似文献
15.
基于线粒体COI基因序列的亚东鲑DNA条形码研究 总被引：7，自引：0，他引：7
以亚东鲑为研究对象,测定分析了线粒体COI基因638bp碱基序列,探讨了该基因作为DNA条形码在鲑科三种鱼类(亚东鲑、大西洋鲑和红点鲑)鉴定方面的可行性和有效性。亚东鲑33个个体共享1种单倍型。利用Kimura-2模型分析得到亚东鲑与大西洋鲑遗传距离最小,为0.079;大西洋鲑与红点鲑遗传距离最大,为0.141;亚东鲑与红点鲑遗传距离为0.120。基于COI基因片段序列构建的NJ显示,亚东鲑和大西洋鲑首先聚到一起,然后再与红点鲑相聚。结果表明COI基因片段作为三种鱼类DNA条形码进行分类鉴定具有一定可行性。 相似文献
16.
Seasonal reproductive and lunar-related spawning cycles of the female honeycomb grouper Epinephelus merra inhabiting Okinawan waters were examined by histological observation of ovaries. Gonadosomatic index (GSI) increased beginning in May and peaked in June. Histological observations revealed that many oocytes laden with yolk were present in the ovaries from May to August. From September to October, ovaries were occupied by immature oocytes. These results suggest that the reproductive season of E. merra lasts for 4 months from May through August in Okinawan waters. When the fish were collected according to the lunar cycle, GSI increased with the approach of the full moon. Oocytes at various development stages were observed from the first quarter to the full moon. Fresh ovulatory follicles were present in the ovaries around the last quarter moon. These results suggest that E. merra has a lunar spawning cycle and spawns between the full moon and the last quarter moon. Ovarian features of the fish collected around the last quarter moon were different among individuals; some fish had many oocytes at the tertiary yolk stage in the ovaries, while the ovaries of the others were occupied by the oocytes at the peri-nucleolus and the oil droplet stages. This observation suggests that a minor release of eggs occurs in this species before or after a major spawning lunar day. Oocytes at the migratory nucleus and the maturation stages were not observed in any ovarian samples. This may mean that maturation of oocytes is related to the spawning behavior of this species and makes rapid progress in the process of aggregation at the spawning sites. 相似文献
17.
为研究云纹石斑鱼(♀)×七带石斑鱼(♂)杂交F1的形态特征以及与其亲本子代的关系,运用常规测量方法和聚类分析法对云纹石斑鱼、七带石斑鱼及云纹石斑鱼(♀)×七带石斑鱼(♂)杂交F1 3个群体6月龄幼鱼的形态特征进行测量和分析,对3个群体的12项可量、可数性状进行指数分析。结果显示,杂交F1体型呈椭圆形,侧扁而粗壮,体侧具7条灰褐色横向条带,杂交F1在体色条纹上均具有云纹石斑鱼与七带石斑鱼的特征;可量、可数的性状中有6项性状偏向于云纹石斑鱼,5项性状偏向于七带石斑鱼,1项与二者相同;聚类分析结果表明,杂交F1与云纹石斑鱼聚为一类。研究表明,杂交F1在体色条纹上表现为二者的中间态,在形态上较接近于母本云纹石斑鱼。 相似文献
18.
W. D. SUMPTON I. W. BROWN D. G. MAYER M. F. McLENNAN A. MAPLESTON A. R. BUTCHER D. J. WELCH & J. M. KIRKWOOD B. SAWYNOK & G. A. BEGG 《Fisheries Management and Ecology》2010,17(1):77-88
Abstract Common coral trout, Plectropomus leopardus Lacepède, crimson snapper, Lutjanus erythropterus Bloch, saddletail snapper, Lutjanus malabaricus (Bloch & Schneider), red emperor, Lutjanus sebae (Cuvier), redthroat emperor, Lethrinus miniatus (Schneider) and grass emperor, Lethrinus laticaudis Alleyne & Macleay, were tagged to determine the effects of barotrauma relief procedures (weighted shot-line release and venting using a hollow needle) and other factors on survival. Release condition was the most significant factor affecting the subsequent recapture rate of all species. Capture depth was significant in all species apart from L. malabaricus and L. miniatus , the general trend being reduced recapture probability with increasing capture depth. Recapture rates of fish hooked in either the lip or mouth were generally significantly higher than for those hooked in the throat or gut. Statistically significant benefit from treating fish for barotrauma was found in only L. malabaricus , but the lack of any negative effects of treating fish indicated that the practices of venting and shot-lining should not be discouraged by fisheries managers for these species. 相似文献