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醛化鸡红细胞应用于鸡新城疫的诊断和抗体检测 总被引：2，自引：0，他引：2
将新鲜鸡红细胞经戊二醛处理后，制成醛化鸡红细胞。该醛化鸡红细胞具有特异性，不发生自凝，保存期可达半年以上，和阴性尿囊液不发生凝集，与新工细胞同时用于血球凝集和血球凝集抑制试验，对疑似鸡新城疫病料和待检测血清进行检测，结果在ＨＡ试验中，新鲜鸡红细胞比醛化鸡红细胞高１－３个滴度，而在ＨＩ试验中，两种红细胞所得结果基本一致，有的则醛化对外开放红细胞比新鲜鸡红细胞高１－２个滴度。由此说明，醛化鸡红细胞完全 相似文献
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植物凝集素 (ｌｅｃｔｉｎ)是一种广泛存在于各种植物性饲料及其原料中的抗营养因子 ,采食过量时动物表现为日增重减少 ,饲料利用率下降 ,并影响免疫系统功能。由于ｌｅｃｔｉｎ具有凝集兔或人等红细胞的能力(凝集作用是凝集素与细胞表面的糖分子结合在细胞表面形成许多交叉的“桥”的结果 ) ,且凝集效价与ｌｅｃｔｉｎ的量成线性关系 ,因此可以利用凝血反应测定其凝集效价 ,然后通过对标准品与待测样品的凝集效价的比较对ｌｅｃｔｉｎ进行定量检测。试验以大豆凝集素 (ＳＢＡ)为研究对象 ,建立了实验室快速检测ＳＢＡ的方法—血凝法。1 材… 相似文献
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山茱萸凝集素的测定及其提取方法的比较 总被引：2，自引：0，他引：2
对药用植物山茱萸进行了血凝活性测定及提取方法的比较.将未经炮制的山茱萸粉碎,用不同的缓冲液在不同的pH和含不同浓度NaCl的浸提液中浸泡并制备浸提液.对不同缓冲液的浸提液分别进行了血凝活性测定.结果表明,山茱萸在1 mol/L NaCl-甘氨酸缓冲液(pH 3)的浸提液中活性最强,并能被N-乙酰葡萄糖胺所抑制;在80%饱和浓度硫酸胺溶液中沉淀最多,活性最强,其活性蛋白含量为4.1%.本试验为开发利用山茱萸,提取其有效成分提供了具体方法. 相似文献
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本试验旨在探讨ＥＤＳ病毒血凝反应的最佳反应条件,观察病毒对各种动物红细胞的凝集谱,测定各种因素对病毒血凝素的影响,结果表明,血凝反应宜采用１％的鸡红血球,稀释液用ｐＨ７的生理盐水,室温作用３５ｍｉｎ（分钟）后观察表明,该病毒能凝集鸡,鸭,鹅红细胞,而不能凝集哺乳动物红细胞,耐热,５６℃１ｈ不影响其血凝性,对胰酶和浓度大于０．４％的甲醛敏感;反复冻融８次对血凝效价无影响。 相似文献
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为观察猪流行性腹泻病毒(PDEV)的血凝特性,进而为建立PEDVHA和HI试验,评估疫苗免疫抗体水平奠定基础,将CV777、DR13、YN13和YN144共4株PEDV毒株,在37℃下,分别用0、5、10、50、100μg/mL胰蛋白酶处理30min,然后用鸡、兔和猪红细胞进行血凝(HA)试验;用已经鉴定为PEDV抗体阳性的血清和乳清分别进行血凝抑制(HI)试验。结果显示:4株PEDV毒株只与兔红细胞发生凝集反应;CV777、DR13毒株的HA现象需要用5μg/mL的胰蛋白酶进行预处理,但是YN13和YN144毒株的HA现象不需要胰蛋白酶作用;该凝集现象能够被PEDV抗体特异性抑制。结果表明,PEDV能够凝集兔的红细胞,不能凝集鸡和猪的红细胞,因而可以用兔红细胞建立PEDV的HA和HI试验方法。 相似文献
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不同醛化剂对红细胞性能及其保存时间的影响 总被引：2，自引：0，他引：2
采用不同醛化剂对新鲜红细胞进行醛化，在同等条件下，测定红细胞的凝集性、溶血性、变形性与时间的关系。实验证明：1％-2．5％的戊二醛对红细胞进行醛化，效果优于甲醛、丙酮，而且醛化后的红细胞不溶血、性能不变、易保存，保存期为120d。 相似文献
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随着实验室检测血清样本数量的增加,延长红细胞的保存时间已成为当前血凝和血凝抑制试验中困扰已久的问题。该试验以新配制的缓冲液稀释保存长达6个月的鸡红细胞不影响血凝和血凝抑制试验结果,这极大方便了实验室及现场血凝、血凝抑制试验的操作。 相似文献
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大豆种子经过浸提、离心、硫酸铵逐级沉淀和D-半乳糖-Epoxy-Sepharose-4B亲和层析,获得单一的大豆凝集素(Soybean agglutinin SBA)样品。纯化的大豆凝集素经pH8.9的聚丙烯酰胺凝胶电泳(胶浓度7.5%),可以显示一条清晰的着色带。在血凝活性鉴定中,采用2%兔细胞悬液,测定凝集红细胞的能力和检测它们与糖结合后对红细胞的凝集作用的影响。结果表明,使兔红细胞50%凝集的蛋白质最低浓度为4μg/mL左右。浓度为0.1mg/ml凝集素溶液分别与D-半乳糖、D-氨基半乳糖、异丙基--βD-硫代半乳糖苷(IPTG)、а—乳糖、几丁质溶液结合后,使兔红细胞50%凝集的糖配体溶液浓度分别为1mg/ml、2.5mg/ml、10mg/ml3、0 mg/ml,而几丁质几乎对SBA没有影响。 相似文献
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鸡传染性支气管炎病毒血凝谱的研究 总被引：1，自引：0，他引：1
用家兔A型魏氏梭菌培养液处理的6株鸡传染性支气管炎病毒,以方阵试验分别与人O型红细胞及羊、猪、兔、鸡、鸭、鹌鹑、麻雀、小鼠等8种动物的红细胞作血凝试验,结果证明,鸡传染性支气管炎病毒H_(120)株和M_(41)株均能凝集人O型以及羊、猪、兔、鸡、鸭、鹌鹑、小鼠的红细胞,而不能凝集麻雀的红细胞;GIBV株和Connecticut株能凝集人O型以及免、鸡、鹌鹑、麻雀的红细胞,而不能凝集猪、羊、鸭、小鼠的红细胞;Gray株能凝集兔、鸡、鹤鹑的红细胞,不能凝集人O型以及猪、羊、鸭、麻雀、小鼠的红细胞;而经家兔A型魏氏梭菌培养液处理的T株和未经处理的6株病毒对人O型以及8种动物的红细胞都没有凝集性。试验还证明,M_(41)株和H_(120)株对人O型及8种动物的血凝活性水平有差异。 相似文献
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The complement fixation (CF) test and the capillary-tube agglutination (CA) test were used to study the antigenic relationship between Babesia bigemina and the large Babesia species frequently infecting cattle in Japan. The CF antigen was prepared from parasitized erythrocytes by extraction with distilled water. The CA antigen was prepared from parasitized erythrocytes by mild sonification of mixtures of Babesia and erythrocyte stroma, following lysis of the erythrocytes with hypotonic saline solution. All the sera used were collected from experimentally-infected cattle. Cross reaction was demonstrated between the Japanese Babesia species and B. bigemina. There was, however, a difference of two dilutions in titer between homologous and heterologous antibody in the CF test, and a difference of more than three tubes in titer between both antibodies in the CA test. It was possible, therefore, to distinguish the Japanese Babesia species from B. bigemina by the CF and CA tests. 相似文献
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Human erythrocytes sensitized with an agglutinating dose of either sheep anti-human erythrocyte antiserum, IgM, slow or fast γ-globulin antibody are haemolysed by foetal lamb or sheep serum complement (C) in the presence of ethyleneglycoltetraacetic acid (EGTA) or Mg2+-EGTA but not ethylenediaminetetraacetic acid (EDTA) or triethylenetetraminehexaacetic acid (TTHA). Dialysis of the C source against phosphate buffered saline rendered it inactive, but addition of Mg2+ or Mg2+-EGTA restored haemolytic activity. Haemolysis of sensitized erythrocytes took place more rapidly in buffer containing Ca2+ and Mg2+ than in EGTA or Mg2+-EGTA. Heating the C source at 50 °C for 30 min or 56 °C for 3 min abolished haemolytic activity. In the presence of EGTA or Mg2+-EGTA, haemolytic activity was restored to 50 °C-heated serum by a CM-cellulose fraction that contained sheep factor B.These results show that sheep C, in the presence of EGTA or Mg2+-EGTA will haemolyse human erythrocytes heavily sensitized with sheep amboceptor. The alternative pathway of sheep C is thought to be involved. 相似文献
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Effects of seven different blood diluents (distilled water, Triton X-100, saponin, isotonic saline solution, pH 7.5 and 8 phosphate buffers and bovine serum albumin) and two chromophores: 5, 5'-dithiobis 2-nitrobenzoic acid (DTNB) and 2,2'-dithiodipyridine (2- PDS) on blood cholinesterase determination in four domestic species (cow, sheep, goat and horse) are described and compared. Haemolytic diluents (distilled water, Triton X-100 and saponin) gave the best precision results when fresh blood was assayed. However, Triton X-100 induced lower ChE activity values in horses, and saponin yielded very high backgrounds in all species tested; so distilled water was recommended as diluent for fresh blood cholinesterase determination. In frozen samples all diluents (except Triton X-100) gave homogeneous final ChE results and showed good between-run precision.Use of 2- PDS as chromophore allowed to do kinetic measurements with approximately 1/3 less haemoglobin interference than when DTNB was employed. This fact allows the use of more concentrated whole blood samples, improving measurements accuracy and decreasing the possible reactivation of inhibited ChE. On the basis of these results, distilled water as diluent and 2- PDS as chromophore are recommended for ChE determination in whole blood. 相似文献
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Erythrocyte osmotic fragility and general health status of adolescent Sprague Dawley rats supplemented with Hibiscus sabdariffa aqueous calyx extracts as neonates followed by a high‐fructose diet post‐weaning 
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下载免费PDF全文 K. G. Ibrahim B. W. Lembede E. Chivandi K. Erlwanger 《Journal of animal physiology and animal nutrition》2018,102(1):114-121
High‐fructose diets (HFD) can cause oxidative damage to tissues including erythrocyte cell membranes. Hibiscus sabdariffa (HS) has protective antioxidant properties. Rats were used to investigate whether the consumption of HS by neonates would result in long‐term effects on their erythrocyte osmotic fragility (EOF) and general health when later fed a high‐fructose diet post‐weaning through adolescence. Eighty of four‐day‐old Sprague Dawley rat pups were divided randomly into three treatment groups. The controls (n = 27) received distilled water at 10 ml/kg b. w, while the other groups received either 50 mg/kg (n = 28) or 500 mg/kg (n = 25) of an HS aqueous calyx extract orally till post‐natal day 14. The rats in each group were weaned and divided into two subgroups; one continued on normal rat chow, and the other received fructose (20% w/v) in their drinking water for 30 days. Blood was collected in heparinised tubes and added to serially diluted (0.0–0.85%) phosphate‐buffered saline to determine the EOF. Clinical markers of health status were determined with an automated chemical analyser. HS extracts did not programme metabolism in the growing rats to alter their general health and EOF in response to the HFD. 相似文献
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D R Pascoe G H Stabenfeldt J P Hughes H Kindahl 《American journal of veterinary research》1989,50(7):1080-1083
Thirty mares with normal estrous cycles were allotted equally to 5 groups and infused with 250 ml of saline (NaCl) solution in utero on the seventh day after ovulation to test the effects of temperature, osmolarity, or pH of the saline solution on prostaglandin F2 alpha (PGF2 alpha) release and luteolysis. Intrauterine infusion of phosphate-buffered saline solution failed to alter the duration of the luteal phase, compared with the control group. Similarly, increasing the temperature of phosphate-buffered saline solution to 42 C or increasing (600 mosm) or decreasing osmolarity (less than 10 mosm) did not change the duration of the luteal phase. Decreasing the pH of saline solution to 3 caused significant (P less than 0.0001) releases of PGF2 alpha from the uterus within the first hour after infusion, and the luteal phase was shortened to 8.8 +/- 1.0 days (mean +/- SEM; control, 15 +/- 1.2 days). The results of this study showed that pH is the main factor in eliciting PGF2 alpha release by intrauterine infusion of a saline solution, whereas increased temperature and osmolarity have no effect on the release of PGF2 alpha. The intrauterine infusion of sterile water or physiologic saline (NaCl) solution has been used to induce estrus in mares for the past 50 years. Many investigators have reported that intrauterine infusion of physiologic saline solution or water at body temperature (37 C) or warmer up to 45 C) causes most "anestrous" mares to return to estrus in 1 to 8 days.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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试验旨在筛选合适的犬红细胞低温保存MAP保存液添加比例。采用前肢内侧皮下头静脉法采集1只健康雄性青背犬的全血,离心收集红细胞,按红细胞与MAP保存液比例为2∶1、2.5∶1、3∶1对犬的红细胞进行4 ℃保存,分别检测第0、10、20、30天时3个比例组的红细胞数目及K+、Na+ 、Cl-浓度。结果表明,2.5∶1保存组的红细胞在低温保存30 d后,红细胞数减少量、K+ 浓度增加量及Na+ 和Cl- 浓度的减少量都最低,与2∶1和3∶1保存组相比均有显著差异（P＜0.05）。因此,红细胞与MAP保存液的比例为2.5∶1时保存效果较好,是犬红细胞低温保存较合适的比例。 相似文献
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本研究旨在探讨香菇多糖对大肠杆菌攻毒大鼠空肠形态结构、上皮细胞数量和紧密连接蛋白表达的影响。将24只SD大鼠随机分成4个组(A、B、C、D组),每组6个重复,每个重复1只。A、B组饮用蒸馏水,C、D组饮用蒸馏水中添加20μg/m L的香菇多糖;试验第15天B、D组灌服2 m L浓度为1×1010CFU/m L大肠杆菌K88,A、C组灌服等量生理盐水。试验第18天心脏采血处死,取空肠固定和冻存,制作石蜡切片,苏木精-伊红(HE)染色和高碘酸雪夫氏(PAS)染色,测定绒毛高度、隐窝深度、绒毛宽度、上皮内淋巴细胞(IEL)数量和上皮杯状细胞(GC)数量,计算绒毛高度与隐窝深度的比值(V/C);并采用蛋白质印迹(Western-blot)法测定空肠紧密连接蛋白Occludin的表达水平。结果表明:各组绒毛高度和绒毛宽度无显著差异(P0.05)。C组隐窝深度极显著低于A、B组(P0.01),显著低于D组(P0.05)。C组V/C极显著高于A、B、D组(P0.01)。C组IEL数量极显著低于B、D组(P0.01),显著低于A组(P0.05)。C组上皮GC数量极显著高于A、D组(P0.01),与B组差异不显著(P0.05)。D组Occludin表达水平明显高于A、B和C组,B组Occludin表达水平高于A组。综上,大鼠饮用水中添加香菇多糖可改善大鼠空肠形态结构,并提高其抵抗大肠杆菌感染能力,促进Occludin的表达。 相似文献
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The hemolytic effect on bovine red blood cells of 5%, 10%, and 15% guaifenesin solutions in 5% dextrose, 0.9% saline (NaCl), or distilled water was determined in vitro at 2 plasma concentrations (250 micrograms/ml, 500 micrograms/ml). A solution of 5% guaifenesin in a 5% dextrose solution or 5% guaifenesin in 0.9% saline produced minimal hemolysis in vitro. The amount of hemolysis of bovine red blood cells in vitro was related to the concentration of guaifenesin, diluent (5% dextrose, 0.9% NaCl, distilled water) and the plasma concentration of guaifenesin. In addition, plasma hemoglobin was determined in 4 adult dairy cows following the IV administration of 5% and 10% guaifenesin. These studies suggest that a solution of 5% guaifenesin in 5% dextrose is the most suitable solution for clinical use in cattle. 相似文献