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异齿裂腹鱼人工规模化繁殖技术研究 总被引：5，自引：1，他引：4
2010年4～6月,对野生异齿裂腹鱼(Schizothorax o' connori)人工规模化繁殖技术进行研究,并初步进行产后亲鱼恢复培养技术研究.对108尾雌鱼进行干法人工授精,共采卵104万多粒,孵出仔鱼62万多尾.其中45尾雌亲鱼自然成熟,共采卵46.8万多粒,平均受精率和孵化率低于人工催产雌鱼卵.人工催产83... 相似文献
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为了进一步了解乐清湾斑鰶的繁殖习性,实现斑鰶苗种规模化人工繁育,于2018年在浙南乐清湾斑鰶繁殖季节捕捞产卵亲鱼,采用湿法人工授精获得受精卵,在室内水泥池进行了育苗试验。人工繁育时海水水温16.3~20.1℃,比重1.017~1.019。育苗饵料系列为轮虫-丰年虫幼体-配合饲料。海上人工授精共获得发育出现胚体的斑鰶受精卵1 998 g,每千克受精卵约53.7万粒。人工授精单日的次日浮卵率最高为13.4%,单日的每千克鱼次日浮卵量最高为15.2 g。2018年共育出平均全长为4.97 cm的斑鰶苗28.16万尾,育苗成活率约为31%。 相似文献
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雄核发育异育银鲫及其初步应用 总被引：2，自引：1，他引：1
用鲫（２ｎ＝１００）卵与异育银鲫（３ｎ＝１５６）正常精子进行人工授精,产生了部分的雄核发育异育银鲫（３ｎ＝１５６）。池塘中饲养的雄核发育能银鲫性腺不仅能成熟,而且也能生殖。它与父本异育银鲫回交可以产生回交子一代（Ｂ１）。雄核发育异育银鲫、回交子一代异育银鲫与父本异育银鲫非常相似。它们均具有相同的染色体数目（３ｎ＝１５６）,红细胞核的体积是鲫红细胞核体积的１．６倍。另一方面,用随机拉增多态ＤＮＡ（Ｒ 相似文献
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我们场进行家鱼人工繁殖，由于设备条件限制，均以人工干法授精获得受精卵。几年来受精率偏低，且不稳定，同时同尾亲鱼，同批卵粒、同质精液授精的卵(分放于几个孵化缸)中，出现不少受精率高低悬殊的情况。为了查明原因，我们今年在排除卵、精子质量和其它因素的前提下，从授精方法的各个环节上着手找问题。通过分析研究，初步认为：过去由于人工授精工作量大，操作工序衔接不协调，盆放卵量太多， 相似文献
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舟山渔场银鲳人工授精及孵化 总被引：10，自引：0，他引：10
2005-2008年,在舟山海域银鲳(Pampus argenteus)繁殖季节(4-5月),随流刺网或张网捕鱼船出海,捕捞性腺成熟的银鲳,进行人工授精和孵化.人工授精试验采用干法、湿法和半干法,比较受精率和孵化率.通过4年连续测量银鲳成熟卵的卵径、油球径等形态指标,对卵子成熟度进行判别,在水温16.0～18.5 ℃、盐度28.0±0.5的条件下进行孵化.结果表明,以干法受精后间隔3～5 min水洗和半干法受精的方法受精率最高,受精率达18.50%～33.50%,最高达40%;孵化率达到43.83%～51.00%,最高达66%.银鲳成熟卵子的卵径为(1.368±0.082)mm,油球径为(0.550±0.031)mm,油球1个.对影响海捕银鲳亲体受精率和孵化率的环境条件也进行了讨论. 相似文献
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The variation of virulence of Renibacterium salmoninarum , the causative agent of bacterial kidney disease (BKD) in salmonid fish, was studied by infecting rainbow trout, Oncorhynchus mykiss (Walbaum), with two isolates (strains 325 and 932) from diseased Atlantic salmon, Salmo salar L., and one isolate (strain 4366) from an apparently healthy Atlantic salmon. Coho salmon, Oncorhynchus kisutch (Walbaum), were injected with the strain 932 to estimate difference in fish species resistance. Fish were removed by random sampling for other study purposes, a study design possible with analysis of lifetime distributions incorporating both sampling-, death- and survival-times. At the end of the experiment, the rainbow trout infected with strains 325, 932 and 4366 had a survival probability of 33%, 51% and 72%, respectively. The coho salmon infected with strain 932 had a 26% survival probability. The strain differences were significant according to the log-rank test, and the risk ratio between the strains ranged from 1·8 to 5·4. The strain from the apparently healthy fish was least virulent. The survival of the fish species was different over time. Rainbow trout were more likely to die early in the time course, but high numbers of coho died later, resulting in an overall risk of mortality of 1·4 in favour of rainbow trout. Differences in virulence may reflect changed selective pressure on R. salmoninarum when introduced from feral stocks into the environment of fish farms. 相似文献
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水硬度对七彩神仙鱼幼鱼发育的影响 总被引：4，自引：0，他引：4
采用不同硬度的水对七彩神仙鱼幼鱼进行饲养。6周龄幼鱼在硬度为7.94°dH±0.30°dH时饲养84d后,比在硬度为14.71°dH±0.23°dH水中的幼鱼个体大,生长速度快。表明较高硬度的水有利于七彩神仙鱼幼鱼的生长发育。 相似文献
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牙鲆刺激隐核虫病的防治 总被引：1，自引：0，他引：1
2005年七、八月份,乐亭、滦南一带多家工厂化牙鲆养殖场发生刺激隐核虫病,此种病虫害发病急、传染快、死亡率高。发生过该病的养殖场牙鲆死亡率一般都在50％～80％之间,这种病害给牙鲆养殖业带来很大损失。 相似文献
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《水生生物资源》2003,16(5):461-465
Anti-proteinase activity was demonstrated in the seminal plasma of cyprinid fish species (bream, chub, ide, dace, asp, goldfish, roach, common carp) using electrophoretic techniques combined with a detection method based on inhibition of bovine trypsin. We found species-specific protease inhibitors in the seminal plasma of cyprinids. At least three bands of protease inhibitors with different migration rates could be identified by native PAGE. Higher variability was characterized for bands with slower migration rates. Visualization of inhibitors after SDS-PAGE under non-reducing conditions allowed estimation of their molecular weights. Apparent molecular weights were within the range of 51–59 and 47–54 kDa for the bands with slower and moderate migration rates, respectively. The molecular weight of fast migration bands for roach and common carp were estimated to 23 and 30 kDa, respectively. Inhibitors of common carp seminal plasma differed in their affinity toward serine proteases. Three inhibitors in common carp seminal plasma could be visualized using cod and bovine trypsin, but only two inhibitors (of high molecular weight) were recognized with chymotrypsin. There were differences in anti-proteinase activity and seminal plasma protein concentration in relation to the origin of common carp seminal plasma (breeding lines) and time of milt collection (spawning vs. post-spawning season). 相似文献
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七种甲壳类水产品脂肪酸组成的分析研究 总被引：5，自引：0，他引：5
水产动物食品中富含ω—3型多稀酸,其中尤其是C_(20:5)(EPA)和C_(22:6)(DHA)对人体有特殊保健功能。本文对七种常见的甲壳类水产品进行脂肪酸组成分析,它们是中国对虾、日本沼虾、安氏白虾、克氏原螫虾、葛氏长臂虾、中华绒螯蟹和三寺疣梭子蟹。测定主要采用黄志斌、李淡秋(1990)的“快速制备脂肪酸甲酯用于气相色谱分析”一文所提供的方法。结果显示,被测样品中不饱和脂肪酸含量高于饱和脂肪酸,而不饱和脂肪酸中多烯酸含量高达60%,且以长碳链(C_(20)、C_(22))ω—3型为特征,表明甲壳类水产品体脂中EPA和DHA含量甚高,超过淡水鱼的一倍,但略逊于海水鱼。值得注意的是,甲壳类水产品中EPA的含量高于DHA的含量,这与鱼类中的情况正好相反。 相似文献
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该研究通过肉眼观察、镜检,进行干露、饥饿、盐度突降、福尔马林等抗性试验,并采用病毒检测等方法,以期建立评估斑节对虾（Penaeus monodon）虾苗活力和质量标准。结果表明,斑节对虾健康虾苗具有趋光性、集群性,体表光洁,肌肉透亮,肠胃食物充盈等特性。测试虾苗干露时间以15min为宜,健康虾苗干露后能立即恢复活力,而病弱虾苗多出现死亡、昏迷现象;虾苗的成活率随饥饿时间的延长而降低,随福尔马林浓度升高和时间延长而降低,随盐度突降幅度增加而降低。健康虾苗能忍受100～200μL·L^-1福尔马林溶液30min,成活率近100％;在盐度20～30下虾苗的成活情况较好,而其在淡水中仅能存活1h。对虾苗进行病毒检测,可以避免养殖中因虾苗携带病毒而可能导致的病毒性疾病的暴发。 相似文献
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鳟鱼发眼卵和旨种运输是养鳟生产的重要环节。发眼卵多在冬季和早春运输,苗种多在晚春和夏季运输。苗种运输大多以鱼篓充氧进行短距离汽车运输,长途运输冷水性鱼苗种的实例很少。本文总结了金鳟和虹鳟苗种长途运输方法,以期与养鳟业界共同交流提高。一、包装材料和方法内包装材料是苗种运输专用塑料袋,规格54×104cm,外包装是泡沫塑料箱,规格63.5×45.5×30．5cm,纸箱规格64×46×32．5cm。塑料袋采取双层式,内装苗种和水共12.5kg,其中苗种0.5-1．3kg,100－2000尾。起运前苗种停食1－2天。运鱼用水水温调至6℃,鱼、水入袋后袋内… 相似文献
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猪的胎盘属于弥散型胎盘,这种胎盘的结构特点和饲养管理的不当,常常导致母猪胎衣不下发生,给生猪的生产繁殖带来极大损失。本文针对母猪胎衣不下发生病因、综合防治进行详细阐述,旨在对预防和治疗胎衣不下能有所帮助。 相似文献