| 雄核发育异育银鲫及其初步应用 |
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| 引用本文： | 俞豪祥.雄核发育异育银鲫及其初步应用[J].水产学报,2000,24(1):17-21. |
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| 作者姓名： | 俞豪祥 |
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| 作者单位： | 上海市水产研究所鱼类品种室,200433 |
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| 基金项目： | 上海市科学技术委员会资助!(鲫的应用研究 )，90 3 2 13 0 5 6号 |
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| 摘 要： | 用鲫（２ｎ＝１００）卵与异育银鲫（３ｎ＝１５６）正常精子进行人工授精，产生了部分的雄核发育异育银鲫（３ｎ＝１５６）。池塘中饲养的雄核发育能银鲫性腺不仅能成熟，而且也能生殖。它与父本异育银鲫回交可以产生回交子一代（Ｂ１）。雄核发育异育银鲫、回交子一代异育银鲫与父本异育银鲫非常相似。它们均具有相同的染色体数目（３ｎ＝１５６），红细胞核的体积是鲫红细胞核体积的１．６倍。另一方面，用随机拉增多态ＤＮＡ（Ｒ
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| 关 键 词： | 雄核发育 异育银鲫 遗传育种 RAPD |
| 收稿时间： | 2014/4/17 0:00:00 |
| 修稿时间： | 2014/4/17 0:00:00 |
The androgenetic allogynogenetic silver crucian carp and its priliminary application |
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| Yu Haoxiang.The androgenetic allogynogenetic silver crucian carp and its priliminary application[J].Journal of Fisheries of China,2000,24(1):17-21. |
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| Authors: | Yu Haoxiang |
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| Institution: | Fish Species Laboratory,Shanghai Fisheries Research Institute, 200433 |
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| Abstract: | In this study part andtrogentic 3n= 156 allogynogentic silver crucian carp( 3n AASCC) using
Carassins auratus eggs artif icially fertilizing with normal sperm from 3n= 156 allogynogenetic silver crucian carp
( 3n= 156 ASCC ) were produced . The 3n ASCC fed in pond can not only rearch sexual maturity , but be
reproduced. Meanwhile, backcrossing females 3n AASCC ( F1 - similar to ASCC) to faternal parent 3n= 156
ASCC could produce f irst backcross generat ion ( B1 ASCC being very similar to faternal parent ASCC) . These
have the same chromosomal numbers, e. g. 3n= 156, and volume of the erythr ocyte nuclei is about 1. 6 time
whichis as big as that of C. auratus . On the other hand, genetic variat ions in females C. auratus , males 3n=
156 ASCC, 3n AASCC, backcross generation B1 and red common carp ( Cypinus carpio red variety) were detected
using random amplifies polymorphic DNA(RAPD) markers. The result of molecular genetic markers indicated by
the RAPD- PCR is consistent with that of the studies on cyto logy and cytogenetics ( chromosomes) . Therefore,
androgenetic 3n= 156 ASCC exists in the part of F1 of C . auratus .. .. 3n= 156 ASCC .. . |
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| Keywords: | androgenesis allogynogenetic silver crucian carp (ASCC) Carassius auratus RAPD |
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