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1997年澳大利亚学者首次报道了一种新的人兽共患病毒病,该病以主要引起猪出现木乃伊化和先天畸形等繁殖障碍、并伴有明显骨骼异常和中枢神经系统恶化为特征,其病原被命名为Menangle病毒(Menanglevirus,MenV)。1发病史1997年4月,澳大利亚新南威尔士州梅那哥(Menangle)附近某猪场 相似文献
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Tioman病毒(TiV)于2001年首次在马来西亚果蝠尿液中分离得到。基因序列和系统发育树分析表明,TiV具有腮腺炎病毒属的特征,与新发现的引起人和猪发病的MenV亲源关系最近,为腮腺炎病毒属的新成员。文章着重对TiV的分子生物学、对人和动物的危害等方面进行了综述。 相似文献
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负股RNA病毒反向遗传学操作及其应用 总被引：2，自引：0，他引：2
负股RNA病毒是动物病毒中重要的一群,包括人和动物许多重要的病原,如流感病毒、麻疹病毒、腮腺炎病毒、呼吸道合胞体、副流感、狂犬病、埃博拉及汉坦病毒等。所有这些病毒均属于基因组分节段(正粘病毒、布尼病毒、嵌砂样病毒属)和不分节段(副粘病毒属、弹状病毒属、丝状病毒属和波纳病毒属)的几个属。同其他RNA病毒和DNA病毒相比, 相似文献
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关于新城疫病毒(NDV)的分类地位,见诸我国有关书刊的归属有风疹病毒属[1]、副黏病毒属[2]、腮腺炎病毒属[3,4]等。现据国际病毒分类委员会(Inter-national Comm ittee on T axonom y of V iruses,ICTV)2002年发布的报告,已归类于副黏病毒亚科下新设的A vu lav irus属[5]。1修改 相似文献
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Philbey AW Kirkland PD Ross AD Field HE Srivastava M Davis RJ Love RJ 《Australian veterinary journal》2008,86(11):449-454
Objective To examine flying foxes (Pteropus spp.) for evidence of infection with Menangle virus. Design Clustered non‐random sampling for serology, virus isolation and electron microscopy (EM). Procedure Serum samples were collected from 306 Pteropus spp. in northern and eastern Australia and tested for antibodies against Menangle virus (MenV) using a virus neutralisation test (VNT). Virus isolation was attempted from tissues and faeces collected from 215 Pteropus spp. in New South Wales. Faecal samples from 68 individual Pteropus spp. and four pools of faeces were examined by transmission EM following routine negative staining and immunogold labelling. Results Neutralising antibodies (VNT titres ≥ 8) against MenV were detected in 46% of black flying foxes (P. alecto), 41% of grey‐headed flying foxes (P. poliocephalus), 25% of spectacled flying foxes (P. conspicillatus) and 1% of little red flying foxes (P. scapulatus) in Australia. Positive sera included samples collected from P. poliocephalus in a colony adjacent to a piggery that had experienced reproductive disease caused by MenV. Virus‐like particles were observed by EM in faeces from Pteropus spp. and reactivity was detected in pooled faeces and urine by immunogold EM using sera from sows that had been exposed to MenV. Attempts to isolate the virus from the faeces and tissues from Pteropus spp. were unsuccessful. Conclusion Serological evidence of infection with MenV was detected in Pteropus spp. in Australia. Although virus‐like particles were detected in faeces, no viruses were isolated from faeces, urine or tissues of Pteropus spp. 相似文献
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H Granzow F Weiland E Mundt B K?llner O Werner 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1999,46(6):411-421
Cells infected by Newcastle Disease Virus were observed to contain both intracytoplasmic and intranuclear inclusion bodies. Ultrastructurally, they consisted of twisted strands of about 18-20 nm diameter resembling nucleocapsids. The presence of these inclusions was detected irrespective of host cell or pathogenicity of the virus. In immunofluorescence and immunogold labelling experiments, these structures were tagged by an anti-P protein monoclonal antibody. In summary, we show that intracytoplasmic and intranuclear inclusion bodies, hitherto used as a taxonomic characteristic for the genus Morbillivirus of the Paramyxoviridae, also occur in a member of the genus Rubulavirus. 相似文献
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Karin Müller Susanne Eder Ulrike Jakop Jürgen Schiller Peter Müller Mohammad Bashawat 《Reproduction in domestic animals》2020,55(Z2):55-60
Cryobanking of gametes in combination with artificial insemination is an essential option to support conservation programmes for endangered and threatened species. About two-thirds of the felid species are classified as ‘near threatened’, ‘vulnerable’ or ‘endangered’ ( www.cites.org ), and mostly, epididymal sperm are collected from euthanized or castrated male felids and cryopreserved. However, epididymal compared with ejaculated and cryopreserved compared with fresh sperm have a limited potential to fertilize if vaginal non-surgical insemination is applied in feline species. Missing or highly diluted seminal fluid in epididymal and cryopreserved sperm, as well as a potential interference of extender ingredients with the natural interactive properties of sperm in the female genital tract is discussed as potential drawback which hampers a proper sperm transit and fertilization besides the limited longevity of cryopreserved feline sperm. Individual components in seminal fluid as well as cryoextenders may adversely alter sperm properties and have a different impact on fertility and preservation success. The identification and investigation of beneficial as well as detrimental components is a precondition to deduce options for improving the process of cryopreservation in felids, particularly, if only epididymal sperm are available. 相似文献
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Prenni JE Avery AC Olver CS 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(1):13-24
Proteomics is a rapidly expanding field of scientific study that combines techniques in protein solubilization and separation, mass spectrometry, and genome and protein database searching. The proteome is most commonly defined as the entire complement of proteins expressed in a given cell type or tissue under a given condition. A proteomics experiment may be as simple as identifying a single protein or as complex as identifying thousands of proteins in a cell lysate. In this review, we describe the general principles of proteomics and its analytic methods and present an example of an experiment to characterize the murine reticulocyte membrane proteome. A brief summary of proteomics applications and their clinical potential and relevance to clinical pathology is also presented. 相似文献
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芦丁的生物活性及其在动物生产中的应用研究进展 《畜牧与饲料科学》2022,43(5):68-73
芦丁别称芸香苷、VP,是一种广泛存在于多种植物中的天然黄酮类化合物,不仅具有抗炎、抗氧化、抗病毒和促生长等诸多生物学功能,而且具有安全、低毒、无生物性残留等特性,具有开发为饲用替抗产品的潜力。对芦丁的理化性质、提取方法、体内代谢吸收及在动物生产中的应用进行了综述,以期为畜牧生产中替抗产品的开发提供新思路。 相似文献
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寻找合适的替代抗生素的物质已成为当前畜牧业研究的热点,三丁酸甘油酯是丁酸的前体物质,具有过胃后在后肠定点缓释的特点,并发挥相应的生理功能。文章综述了三丁酸甘油酯的生理功能如保护肠黏膜、促进肠道发育、增强肠道免疫力、缓解炎症、调节胰岛素和脂质代谢、维持肠道菌群稳态、抗癌等,以及在猪生产上的应用,以期为三丁酸甘油酯在养猪生产中作为替抗物质提供参考。 相似文献
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Karen M. Bayne Veronica R. Clifford Brenda R. Baillie H. Grant Pearce 《Strength and Conditioning Journal》2019,72(3):523-532
Fire has long been used as a tool to remove pests and disease, regenerate land, and remove unwanted vegetation buildup. Although traditionally used as a widespread land management tool in the past, its continued use could be under threat due to public perceptions pertaining to risks from burns getting out of control, as well as the impacts of smoke pollution and ecological damage. To determine the attitudes of rural practitioners and residents toward use of fire as a land management tool, this paper reports the results, analysis, and interpretation to a survey question: “Do you feel fire is a good option for managing rural land?”, along with an analysis of key attitudes toward the use of fire as a land management tool. The survey was undertaken with 696 respondents consisting of (rural land managers, rural populace, and rural fire personnel) within New Zealand. The survey identified differing perceptions about the suitability of prescribed fire use by land-based sectors. Five constructs explained the attitudes toward use of fire as a land management tool: benefits of fire use, the tradition of fire as a tool, regulations and liabilities, smoke impacts from fire, and the risks from knowledge loss and changing land use. Results showed that a person’s high agreement with the construct concerning negative smoke impacts resulted in less agreement that fire was a good option for use in rural land management in New Zealand. Main demographics influencing a positive attitude toward the use of fire included gender (male), their current use of fire, and larger land area managed. For prescribed fire to continue to be a useful tool, an improved understanding of current management practices and clear guidelines around best practice in using fire in New Zealand to manage land are required, with particular emphasis on limiting smoke impacts. 相似文献
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