共查询到20条相似文献，搜索用时 31 毫秒
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以２,４－Ｄ处理玉米苗并接种根瘤菌,诱导根瘤菌与玉米苗结瘤共生．试验结果如下：５个玉米品种的始瘤期仅需２～３ｄ,结瘤率高达９０％～１００％,固氮酶活性（乙炔还原法）在４５～５５ｎｍｏｌ株－１ｄ－１之间．在闽单８８中,还出现琼脂液化、结红瘤等特殊现象．经显微镜观察表明,根瘤菌可从弯曲、膨大、变形的根毛侵入,可形成类侵入线;瘤组织细胞内含有数量众多的根瘤菌菌体．说明了２,４－Ｄ能诱导根瘤菌进入非豆科植物根瘤,并能在瘤内繁殖． 相似文献
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V. N. Zolotarev 《Russian Agricultural Sciences》2016,42(1):46-49
The responsiveness of common vetch varieties to inoculation with Rhizobium leguminosarum biovar-viciae of different genotypes and applications of molybdenum and boron micronutrients has been revealed. It is shown that the effect of interactions between molybdenum and boron on the plants at the joint application is nonadditive. The artificial colonization of seeds by the complementary strains of root nodule bacteria and the molybdenum treatment are expedient to increase the efficiency of the symbiotic interactions between rhizobia and common vetch varieties. Inoculation with strain no. 145 and supplementation with molybdenum improved the seed yields of the common vetch Valentina and Lugovskaya-98 varieties by 20% and 17%, respectively. 相似文献
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A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes 总被引：108，自引：0，他引：108
The symbiotic interaction of Rhizobium meliloti and alfalfa results in the formation of nitrogen-fixing root nodules. Rhizobium meliloti nodABC genes are required for the early host responses of cortical cell divisions and root hair curling. The induction of nodABC expression by alfalfa exudates demonstrates host-symbiont signaling at an early stage in nodule development. The inducer molecule for nodABC expression was isolated from plant exudate by constructing a nodABC-lacZ fusion to monitor the inducing activity. From ultraviolet-visible absorption spectra, proton nuclear magnetic resonance, and mass spectrometry, the inducer was determined to be 3',4', 5,7-tetrahydroxyflavone (luteolin). Luteolin is a normal secondary plant metabolite found throughout the plant kingdom that may serve to control nodABC expression during nodule development. This regulatory role for a flavone contrasts with the function of some flavonoids as defense compounds. 相似文献
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Murray JD Karas BJ Sato S Tabata S Amyot L Szczyglowski K 《Science (New York, N.Y.)》2007,315(5808):101-104
In legumes, Nod-factor signaling by rhizobia initiates the development of the nitrogen-fixing nodule symbiosis, but the direct cell division stimulus that brings about nodule primordia inception in the root cortex remains obscure. We showed that Lotus japonicus plants homozygous for a mutation in the HYPERINFECTED 1 (HIT1) locus exhibit abundant infection-thread formation but fail to initiate timely cortical cell divisions in response to rhizobial signaling. We demonstrated that the corresponding gene encodes a cytokinin receptor that is required for the activation of the nodule inception regulator Nin and nodule organogenesis. 相似文献
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豆科植物根瘤形成条件初探 总被引：5，自引：0，他引：5
豆科植物的根瘤可以从空气中固定氮气而在贫瘠的土壤中生长。它的形成需要土壤中的根瘤菌或人工培养的根瘤菌与植物共同形成。但根瘤的形成还可由培养根瘤菌的无菌滤液诱导形成,这充分说明根瘤菌生长过程中分泌的某种可溶性物质具有刺激植物形成根瘤的作用,但这种根瘤由于不能形成豆血红蛋白而呈淡绿色或白色,且根瘤中有大量淀粉积累。实验中通过对根瘤的接种、观察、分析,总结了根瘤形成的原因,使我们对根瘤的研究更加深入。 相似文献
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不同紫云英品种与根瘤菌匹配对结瘤固氮的影响 总被引：1，自引：0，他引：1
研究不同紫云英品种与根瘤菌匹配后对紫云英的结瘤固氮影响。结果表明：M10根瘤菌种可分别显著提高8410441、闽紫6号、弋江籽的固氮酶活性、紫云英根瘤数、紫云英根瘤鲜重;ZLH2根瘤菌显著提高8410441紫云英地上部鲜重,ZK7根瘤菌提高闽紫1号紫云英全氮含量。从总固氮量来看,M10、XZ、ZLH2、ZK7、M3根瘤菌分别对提高闽紫6号、8324411、8410441、余江大叶、弋江籽品种的总固氮量效果最佳,其中以ZLH2对8410441的总固氮量最高。固氮酶活性与根瘤数呈极显著负相关,根瘤数与根瘤重呈极显著正相关,产量、全氮含量与总固氮量呈极显著正相关。通过多指标综合评价发现,ZLH2对闽紫6号、8410441的促生效果较好;M10对8324411和弋江籽的促生效果较好;ZK7对余江大叶的促生效果较好。 相似文献
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为建立水稻-根瘤菌共生固氮体系,将百脉根共生信号转导途径中的4个功能基因(LHK1、NSP1、NSP2和NIN)转入水稻,获得稳定转化的转基因水稻,并初步检测转基因水稻的表型和转录组水平上的差异。根瘤菌感染试验结果显示,根瘤菌侵染对照(转入空载体)和转基因水稻的根毛和表皮的比率没有显著变化,但接种根瘤菌14d后,对照与转基因水稻的侧根原基数有显著差异,转共生基因水稻的侧根原基明显增多。利用水稻cDNA芯片,检测转基因水稻组与对照组在接种根瘤菌7d和不接种根瘤菌条件下根的转录组变化,结果显示,转基因组与对照组水稻在植物防御反应、激素信号转导途径相关基因均有差异性表达,表明豆科共生基因影响了水稻对根瘤菌的反应。 相似文献
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In order to produce efficiently symbiotic system in acid soils which could be used for the recovery of insoluble phosphate soil and improvement of the fertility,etc.,host range of Rhizobium was isolated from the root nodules of wild and cultivated soybean varieties growing in south China,evaluated the effects of Rhizobium on enhance nitrogen fixing activities,phosphorus(P),nitrogen (N)uptakes,growth and yield of soybean under pot and field conditions.The results showed that combined Rhizobium inoculation was more effective than individual Rhizobium inoculation. The combined Rhizobium of CW54 strains had the highest effect, nodule number, nodule weight and nodule nitrogenase activity were markedly stimulated as compared to those of the control U110, respectively. The nitrogen contents in the plants treated with CW54 strains were significantly increased by 43.2% as compared to those of the control U110 in Al-P soil, but statistical significance was not observed in Fe-P soil. By contrast, inoculation with CW54 significantly enhanced phosphorus uptake of plant in Fe-P soil than that in Al-P soil. The effects of inoculation of CW54 strains in a field condition were similar to those of in a pot condition. The total biomass and yield in the plants treated with CW54 strains were significantly increased by 86.4% and 51.4% as compared to those of the control at the time of soybean podding in a field condition, respectively.These results suggested that inoculation Rhizobium was beneficial for soybean nutrient uptake,growth and yield under insoluble phosphate soil conditions. 相似文献
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Nodulin-35, a 35,000-molecular-weight protein, is present in soybean root nodules developed by different strains of Rhizobium japonicum, irrespective of their effectiveness in fixing atmospheric nitrogen. This protein is not detected in uninfected plants and bacteroids or in free-living Rhizobium and appears to be synthesized by the plant during the formation of root nodules. 相似文献
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大豆可与根瘤菌互作进行共生固氮,有效缓解其对土壤氮素的依赖。细胞色素P450(cytochrome P450,CYP450)是一种血红素单加氧酶,参与植物信号分子、植物激素和黄酮类等多种次生代谢物的合成,为探究其在大豆结瘤固氮中的生物学功能,分析了该家族GmCYP78A71基因的结构,并对其表达模式及生物学功能进行了深入解析。qRT-PCR和转启动子大豆毛根的根瘤GUS染色表明,GmCYP78A71在根瘤中特异表达,且随着根瘤的发育表达量逐渐增高,在成熟根瘤中达到峰值;与对照相比,超表达复合植株的根瘤个数、鲜重、固氮酶活性等显著提高,而RNAi毛状根的根瘤个数、鲜重、固氮酶活性等显著降低。上述结果表明GmCYP78A71对根瘤的生长发育和固氮发挥重要作用。 相似文献
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硝态氮与铵态氮对紫花苜蓿根系生长及结瘤固氮的影响 总被引：2，自引：4，他引：2
以‘甘农3号’紫花苜蓿为材料,采用砂培方法,研究了硝态氮和铵态氮对紫花苜蓿根系生长、根系活力、地上部和地下部全氮含量、根瘤数量和固氮酶活性的影响.结果表明:铵态氮培养下,‘甘农3号’紫花苜蓿的根系及根瘤生长、根系活力、地上部和全株全氮含量、固氮酶活性均好于硝态氮培养;在不同形态氮素培养下接种根瘤菌,紫花苜蓿的根系活力、... 相似文献
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为研究ROP(Rho-related GTPase of plants,ROP)在豆科植物共生固氮过程中的功能和作用机制,从百脉根逆转录转座子LORE1插入突变体库(http://www.kazusa.or.jp/lotus)中搜索到6个ROP相关基因突变体,通过分离和鉴定,得到不同突变体的M1代和M2代纯合体种子,对M2代进行表型鉴定及统计分析。结果表明:在早期共生表型的鉴定中,突变体与野生型植株相比,rop-like1植株的根瘤原基数明显下降,rop-like4植株的侵入线数和侵入线密度也明显下降;但仅rop-like1植株在接种14d后的结瘤数明显减少。 相似文献
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毛萼田菁茎瘤的形成和固氮活性的研究 总被引：3，自引：0，他引：3
毛萼田菁(S.rostrata)喜高温、高湿生态环境,在温度20℃以上、相对湿度70—80％的条件下生长迅速,植株可达3.5米以上。 毛萼田菁的茎瘤形成于茎上蛰伏的不定根发生点。当合适的根瘤菌从不定根发生点裂口入侵后,刺激不定根原基周围的皮层细胞分裂形成含菌组织而发育成茎瘤。茎瘤的固氮活性随茎瘤和其中类菌体的发育和衰败而升降。植株的共生固氮周期呈抛物线。茎瘤的固氮活性为根瘤的2.3倍,单株茎瘤的固氮量为根瘤的46倍。植物的光合作用对茎瘤的形成和固氮强度有决定性影响。在正常光照下发育的茎瘤的固氮活性约为包扎遮光下发育的茎瘤固氮活性的1.5倍。 相似文献
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Soybean lectin labeled with fluorescein isothiocyanate combined specifically with all but 3 of 25 strains of the soybean-nodulating bacterium Rhizobium japonicum. The lectin did not bind to any of 23 other strains representative of rhizobia that do not nodulate soybeans. The evidence suggests that an interaction between legume lectins and Rhizobium cells may account for the specificity expressed between rhizobia and host plant in the initiation of the nitrogen-fixing symbiosis. 相似文献
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河北地区花生根瘤菌的系统发育多样性研究 总被引：2，自引：0，他引：2
【目的】了解中国北方地区花生根瘤菌的多样性状况。【方法】采用16S rDNA PCR-RFLP、16S～23S IGS PCR-RFLP和16S rRNA基因的序列分析方法对分离自河北地区的58株花生根瘤菌以及Rhizobium、Agrobacterium、Sinorhizobium、Mesorhizobium和Bradyrhizobium的参比菌株进行系统发育多样性比较分析。【结果】16S rDNA PCR-RFLP方法在细菌属水平上聚群良好；而16S～23S IGS PCR-RFLP则适于属和种水平上的聚群。3种方法在系统发育上得到基本一致的结果。其中有38株花生根瘤菌是与Bradyrhizobium关系密切的慢生菌株，并且与B. japonicum和B. liaoningense的关系最为接近，与有关中国西南地区和华中地区花生根瘤菌的报道结果相似。然而供试菌株中还存在6株快生和10株中慢生花生根瘤菌，其中快生菌在系统发育关系上接近R. yanglingense、R. mongolense和R. gallicum。【结论】河北地区的花生根瘤菌具有更大的多样性。 相似文献
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A protein that specifically agglutinates Rhizobium meliloti, the alfalfa root nodule endosymbiont, has been purified from alfalfa seed. Material cross-reactive to antiserum prepared against the purified agglutinin is present in all alfalfa varieties that were tested but is absent in corn and other legumes not nodulated by Rhizobium meliloti. Studies with nonnodulating mutants of this microorganism incapable of binding to alfalfa roots suggest that the agglutinin is responsible for specific recognition between Rhizobium meliloti and alfalfa and that this recognition is an essential step in nodule formation. 相似文献