共查询到18条相似文献，搜索用时 859 毫秒
1.
为了探讨复方参芩对犬细小病毒致犬心肌组织Bcl-2和Bax基因表达的影响,将丹参、黄芩、甘草等中药配伍并制备成为复方参芩针剂,人工感染犬细小病毒建立模型;将犬分为空白对照组、模型组、黄芪多糖组、复方参芩组;给药7d后,接种病毒,观察各组犬临床症状,取心肌组织,电镜观察心脏组织超微结构变化,采用荧光实时定量PCR法检测Bcl-2和Bax mRNA表达.结果表明,模型组犬死亡率高,心肌组织结构损伤严重,与空白对照组比较,心肌组织细胞Bcl-2 mRNA表达下调(P＜0.05),Bax mRNA表达增加(P＜0.01).复方参芩组犬存活率较高,心肌组织损伤轻,与模型组相比,心肌组织细胞Bax mRNA表达下调(P＜0.01),Bcl-2 mRNA表达上调(P＜0.01).通过本试验证明复方参芩可通过上调犬心肌组织细胞Bcl-2 mRNA表达,下调Bax mRNA的表达,抑制细小病毒引起的心肌细胞凋亡,保护犬心肌组织免受细小病毒损害. 相似文献
2.
3.
将犬分为空白对照组、模型组、复方苦芩预防组、复方苦芩治疗组,用犬细小病毒接种建立动物模型,复方苦芩防治犬细小病毒病,然后取样,光学显微镜和电子显微镜观察十二指肠黏膜细胞的病变和细胞凋亡,逆转录聚合酶链式反应（RT-PCR）法检测Bcl-2和Bax基因的mRNA表达。结果显示,与空白对照组比较,模型组犬十二指肠黏膜病变及炎细胞浸润,电镜下可见细胞收缩,核浓缩,深染,线粒体肿胀空化,细胞Bcl-2基因表达下调,Bax基因表达增加。与模型组相比,复方苦芩防治组肠黏膜病变轻,超微结构变化不明显,细胞Bax基因表达下调,Bcl-2基因表达上调。结果表明,复方苦芩可能是通过促进黏膜上皮细胞的增殖与恢复,调节Bcl-2,Bax基因表达,抑制犬细小病毒引起的十二指肠黏膜超微结构改变和细胞凋亡,而对细小病毒病犬的十二指肠组织结构起保护和促进恢复作用。 相似文献
4.
犬细小病毒所引起的犬细小病毒病是危害养犬业最为严重的传染病之一,临床以剧烈呕吐、出血性肠炎、心肌炎和白细胞显著减少为主要特征,感染发病率高,传染性强,病死率高,本文浅析了犬细小病毒的生物学特性,细小病毒病临床症状、诊断、治疗和愈后、预防等内容,以期对犬细小病毒病的防治提供一定的参考。 相似文献
5.
犬细小病毒病是由犬细小病毒引起的急性传染病,以剧烈呕吐、出血性肠炎、心肌炎为主要特征,是目前犬病毒性腹泻的主要原因之一。接种疫苗是预防犬细小病毒病的最有效途径。一旦犬发生免疫失败感染犬细小病毒病时,应采取相应的防治措施,以降低损失。笔者通过一典型病例的诊疗过程,对犬细小病毒病的发病、诊断及防治措施进行了介绍,以期为犬细小病毒病的诊治提供参考。 相似文献
6.
《中国兽医学报》2019,(3):486-492
为探讨复方苦芩3种有效活性成分对人工感染肠炎型犬细小病毒病(canine parvovirus disease,CPVD)病犬小肠组织的保护作用,将50只试验犬随机分为复方苦芩总多糖组、复方苦芩总生物碱组、复方苦芩总黄酮组、空白对照组和模型组。其中,试验药物组均按1.5 mL/kg肌肉注射复方苦芩3种有效成分,2次/d,连续用药7 d后灌服2 mL(5×10~5 PFU/mL)的病毒液进行人工复制病理模型,空白组肌注射生理盐水,模型组只攻毒不给药。接种病毒后经检测为阳性后3天在无菌条件下取各组犬只小肠组织,用TUNEL法检测各组小肠组织细胞的凋亡率,同时采用荧光定量PCR对各试验组小肠组织Bax和Bcl-2 mRNA转录进行相对定量检测,并运用Western blot方法检测Bcl-2和Bax蛋白表达量。结果显示,通过组织切片观察,复方苦芩3种有效活性成分可防止病毒对犬小肠细胞形态的病理作用,可降低细胞凋亡率,并且3种有效成分对小肠组织可增高小肠组织Bcl-2/Bax基因和蛋白表达量,从而减少因病毒所引起的细胞凋亡;结果表明,复方苦芩3种有效成分对CPVD病犬小肠组织均有一定的保护作用,其中复方苦芩总生物碱对CPVD病犬小肠组织细胞凋亡的预防效果最显著。 相似文献
7.
犬细小病毒病临床上以急性出血性肠炎和非化脓性心肌炎为特征,常呈暴发性流行,其感染率可达100%,致死率为10%~50%,急性病例病程通常不超过24h,犬细小病毒对不同年龄、不同性别、不同品种的犬均可感染,尤以断乳至90日龄的犬多发,病情严重.京巴和德牧为高发品种,2~6月龄犬高发,疫苗免疫对预防犬细小病毒病的发生有显著效果,采用合理的治疗方案后,肠炎型初期的病例很高.对犬细小病毒病的流行情况采取合理的预防、治疗有一定的作用. 相似文献
8.
9.
10.
犬细小病毒（CPV）感染是近年来发现的犬的一种高度接触性传染病。犬细小病毒病发病急,死亡率高,常呈爆发性流行。临床表现以急性出血性肠炎和非化脓性心肌炎为特征。CPV感染后以下消化道和心脏病理变化为主。使用CPV测试板可确诊。本病目前没有特效治疗药物，虽然治愈率可达80％～90％，但由于犬主对病情的不了解而延误治疗，导致目前治愈率并不高。因此进行免疫接种是预防犬细小病毒病的有效途径。 相似文献
11.
Maxson TR Meurs KM Lehmkuhl LB Magnon AL Weisbrode SE Atkins CE 《American journal of veterinary research》2001,62(1):130-135
OBJECTIVE: To perform polymerase chain reaction (PCR) analysis on paraffin-embedded myocardium from dogs with dilated cardiomyopathy (DCM) and dogs with myocarditis to screen for canine parvovirus, adenovirus types 1 and 2, and herpesvirus. SAMPLE POPULATION: Myocardial specimens from 18 dogs with an antemortem diagnosis of DCM and 9 dogs with a histopathologic diagnosis of myocarditis were evaluated. PROCEDURE: Paraffin-embedded myocardial specimens were screened for viral genome by PCR analysis. Positive-control specimens were developed from cell cultures as well as paraffin-embedded tissue specimens from dogs with clinical and histopathologic diagnoses of viral infection with canine parvovirus, adenovirus types 1 and 2, and herpesvirus. The histologic characteristics of all myocardial specimens were classified regarding extent, location, and type of inflammation and fibrosis. RESULTS: Canine adenovirus type 1 was amplified from 1 specimen from a dog with DCM. Canine parvovirus, adenovirus type 2, and herpesvirus were not amplified from any myocardial specimens. Histologic analysis of specimens from dogs with DCM revealed variable amounts of fibrosis; myocardial inflammation was observed in 1 affected dog. Histopathologic analysis of specimens from dogs with myocarditis disclosed variable degrees of inflammation and fibrosis. CONCLUSIONS AND CLINICAL RELEVANCE: Viral agents canine parvovirus, adenovirus types 1 and 2, and herpesvirus are not commonly associated with DCM or active myocarditis in dogs. Additional studies evaluating for nucleic acid from viruses that less commonly affect dogs or different types of infectious agents may be warranted to gain insight into the cause of DCM and myocarditis in dogs. 相似文献
12.
犬细小病毒病流行病学调查 总被引：2，自引：1，他引：1
犬细小病毒感染是由犬细小病毒引起的一种急性、热性、传染性疾病,临床上以出血性肠炎或心肌炎为主要特征。本研究对泰州某宠物门诊2010年1月－2011年3月期间接诊的260例犬细小病例进行了分析研究,旨在了解泰州地区犬细小病毒病发病率、死亡率与犬年龄、品种、免疫接种、季节等因素之间的关系,并总结防控犬细小病毒病最有效的方法。结果表明,犬细小病毒病发病率及死亡率与犬的年龄、品种、免疫接种、季节有很大的相关性,依此提出了防制对策,以期为防制犬细小病毒病提供参考。 相似文献
13.
Mueller RS Fieseler KV Bettenay SV Rosychuk RA 《American journal of veterinary research》2002,63(4):491-494
OBJECTIVE: To evaluate the effect of long-term treatment with tetracycline and niacinamide on antibody production in dogs by measuring postvaccinal serum concentrations of antibodies against canine parvovirus and canine distemper virus. ANIMALS: 10 dogs receiving long-term treatment with tetracycline and niacinamide (treatment group) and 10 healthy dogs (control group). PROCEDURE: The treatment group included 9 dogs with discoid lupus erythematosus and 1 dog with pemphigus foliaceus on long-term treatment (> 12 months) with tetracycline and niacinamide. The control group included 10 healthy dogs with no clinical signs of disease and no administered medications for the past 3 months. Blood samples were obtained from all dogs by jugular venipuncture. Serum antibody titers against canine parvovirus and canine distemper virus antigens were measured, using hemaglutination inhibition and serum neutralization, respectively, and compared between groups. RESULTS: A significant difference in antibody titers between treatment- and control-group dogs was not found. All dogs had protective antibody titers against canine distemper virus, and 8 of 10 dogs from each group had protective titers against canine parvovirus infection. CONCLUSION AND CLINICAL RELEVANCE: These results provide evidence that long-term treatment with tetracycline and niacinamide does not interfere with routine vaccinations and thus does not seem to influence antibody production in dogs. 相似文献
14.
犬细小病毒病是由犬细小病毒(CPV)感染幼犬引起的一种急性传染病,以剧烈的呕吐、出血性肠炎、白细胞显著减少以及心肌炎为主要特征,发病率高、传染性强、死亡率高,是危害我国养犬业最为严重的传染病之一。通过调查研究,揭示了山东寿光地区犬细小病毒病的流行特点,以期对今后犬细小病毒病的临床诊疗防治具有一定的指导意义。 相似文献
15.
16.
17.
Blanco PG Batista PR Re NE Mattioli GA Arias DO Gobello C 《Reproduction in domestic animals》2012,47(2):252-256
The aim of this study was to describe the canine electrocardiographic changes in the course of normal and abnormal pregnancy. Twenty-three Brucellosis-negative pregnant bitches were retrospectively classified as normal (n = 12) or abnormal (n = 11). A control group of non-pregnant dioestrous bitches (n = 10) was also included. Normal pregnant females delivered healthy puppies at term while abnormal animals interrupted their pregnancy between days 52-60 (from estimated luteinizing hormone peak) or presented perinatal litter death higher than 60%. All the bitches were electrocardiographically evaluated every 10 days from day 0 to day 65 of the oestrous cycle, to parturition or abortion. Percentage heart rate change increased 31.3% from day 40 to 60 in normal gestation while it decreased -1.8% in dioestrous bitches, although it did not change in the abnormal group (p < 0.01). In the abnormal pregnant group but not in the others, percentage QRSa change fell to -34% on day 60 (p < 0.01). At the same time point, percentage QRSd change was 6.2% vs -4.9% in normal gestations and dioestrous animals, respectively (p < 0.05). Corrected QT interval augmented from day 40 onwards up to 9.9% and 4.3% in the normal pregnant and dioestrous groups, respectively, while it remained unchanged in abnormal gestations (p < 0.05). It is concluded that during normal canine pregnancy, some electrocardiographic parameters begin changing from day 40 onwards, and that pathological gestations differ from normality from day 30. The use of electrocardiography in canine obstetrics might contribute to identify abnormal outcomes before they become clinically evident. 相似文献
18.
M A Hayes R G Russell L A Babiuk 《Journal of the American Veterinary Medical Association》1979,174(11):1197-1203
Sudden death of pups in the 4- to 6-week age range has recently been occurring in western Canada as a result of severe, primary, nonsuppurative myocarditis. At necropsy, the prominent macroscopic lesion was pulmonary edema, and microscopically, characteristic intranuclear inclusion bodies were found within cardiac myofibers in association with myocarditis. Ultrastructurally, numerous small particles resembling parvoviruses were found within the intranuclear inclusion bodies, which were positive by direct fluorescent antibody test for canine parvovirus. Of three pups inoculated with homogenate from affected myocardium, one developed lesions resembling canine parvoviral enteritis. 相似文献