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1.
为了探索中国大蒜种质个体的SSR位点的分布情况,为品种鉴定、保存及遗传改良提供分子生物学依据,利用6对SSR引物对40个大蒜(Allium sativumL.)品种进行聚类分析、主成分分析及遗传多样性评价。共检测到21个多态性位点,平均每对引物可扩增出约3.5条多态性片段,多态性百分率为56.76%;SSR引物组合平均有效等位基因数、Nei基因多样度和Shannon信息指数分别为1.5551、0.3414和0.5188。聚类分析显示,6对SSR引物可把40份大蒜种质资源从0.59相似系数水平上3个类群。第一类群包含28份种质,在相似系数为0.73的水平上进一步又被分成了3个亚类;第二亚类仅包含2份种质;第三亚类包含10份种质,在0.68的相似系数水平上分成了2个亚类。主成分分析和UPGMA的结果基本一致。不同地理来源的大蒜种质的Shannon-Weaver多样性指数的变幅为0.0576~0.4179,说明大蒜种质遗传多样性丰富。本研究利用SSR分子标记技术较准确地解析大蒜不同材料间的亲缘关系及遗传多样性,为中国大蒜SSR分子标记提供基础资料。 相似文献
2.
对鸡脂肪性状候选基因进行连锁不平衡(linkage disequilibrium,LD)分析可以为理解鸡体脂沉积的遗传学机制提供重要信息,并且可以提高脂肪性状相关分子遗传标记的检出效率.本研究以东北农业大学肉鸡高、低脂双向选择品系第8世代肉仔鸡为实验材料,通过直接测序、高效液相色谱(DHPLC)、单链构象多态性(PCR-sscv)、限制性片段长度多态性(PCR-RFLP)、长度多态性(PCR-LP)等方法对过氧化物酶增殖体激活受体(PPAR-γ)、载脂蛋白B(ApoB)、解耦联蛋白(UCD)以及其他10个鸡脂肪性状重要候选基因进行多态性检测,计算候选基因标记间LD参数∣D'∣与r2,分析这些候选基因标记间的LD模式并探讨其规律.初步发现了以下特征:1、在各候选基因内,标记间的∣D'∣与r2值并不匹配,取值区间差异很大,∣D'∣值普遍大于r2;另外,在有些基因内的相同标记间,∣D'∣、r2值相差很大,甚至在∣D'∣=1时,r2接近于0.2、在不同基因甚至在同一基因内不同区域LD水平差异很大且物理距离与LD关系并不成线性关系.3、在有些基因内,如ApoB基因,相间位点间的∣D'∣和r2值大于相邻位点间的值.4、有些基因,如PPAR4-γ,相同标记间LD水平在高、低脂系间存在很大差异.本研究结果提示,在群体遗传学研究中进行候选基因LD分析时,LD参数r2的应用价值比∣D'∣更大. 相似文献
3.
山东省46个花生品种SSR指纹图谱构建与遗传多样性分析 总被引：2，自引：0，他引：2
为从分子水平上快速鉴别花生品种和选配优良杂交组合,以山东省审定的46个花生品种为材料,利用微卫星(SSR)标记进行DNA指纹图谱的构建和遗传多样性分析。从788对SSR引物中筛选出50对多态性高、稳定性好、谱带清晰的引物,共检测到175个等位位点,其中122个为多态性位点,多态性比率达70.52%;每对SSR引物扩增出的等位位点数为2~7个,多态性信息量变化范围为0.6753~0.8412,平均为0.823。此外,利用14对引物可将46份材料完全区分开。聚类分析表明,在相似系数0.77处,所有供试材料聚为一类,在相似系数0.80处,仍有76%的材料聚在一起。利用SSR标记构建的指纹图谱可为花生种质资源管理及育种实践提供依据。 相似文献
4.
金针菇种质资源5个农艺性状与SSR标记的关联分析 总被引：2，自引：0，他引：2
金针菇(Flammulina velutipes)是一种重要的商业化栽培食用菌,为挖掘控制金针菇重要农艺性状的基因组区段,本研究以90份金针菇种质资源为实验材料,测定其原基期、菌柄直径、菌柄长度、菌盖直径和单瓶产量5个农艺性状,利用全基因组序列开发的95份多态简单重复序列(simple sequence repeat,SSR)标记对供试材料进行基因组扫描,在分析实验材料的群体结构和连锁不平衡基础上,采用一般线性模型方法对5个农艺性状进行关联分析。结果表明,各供试材料的5个农艺性状均有极显著差异。95份多态SSR标记共检测到582个等位变异,平均每个位点的等位变异数为6.13个。标记多态信息含量(polymorphism information content,PIC)变化范围为0.204~0.818,平均为0.542。群体遗传结构分析将供试材料分为8个亚群体,群体内SSR位点间存在较高的连锁不平衡(linkage disequilibrium,LD),不平衡程度D’值大于0.5的组合数占总组合数的33.01%。关联分析检测出28个标记与5个性状显著相关,其中原基期的关联标记有2个,菌柄直径的关联标记有12个,菌柄长度的关联标记有3个,菌盖直径的关联标记有9个,单瓶产量的关联标记有5个。关联SSR标记对表型变异解释率的变幅为5.11%~23.55%。研究表明,所选金针菇种质资源群体的遗传多样性以及连锁不平衡程度较高,适用于关联分析研究。本研究结果对金针菇核心种质资源库构建以及分子标记辅助育种具有参考价值。 相似文献
5.
大麦SSR标记遗传多样性及群体遗传结构分析 总被引：1，自引：0，他引：1
为了确定大麦亲本材料间的亲缘关系,为后期关联分析奠定基础,本研究利用71个SSR标记对不同来源的99份大麦材料进行了遗传多样性及群体遗传结构分析。结果表明,71个SSR标记共检测到184个等位变异,变幅为2-5个,平均每个标记有2.6个。Shannon指数变幅为0.0565~1.2241,平均值为0.6086。标记多态性信息含量(PIC)的变幅介于0.0200~0.6633之间,平均值为0.3729。聚类结果表明,试验品种的遗传相似系数(GS)的变异范围为0.5109~0.9511,平均值为0.7202。GS值在0.7100水平上分为5大类,分别包括5、4、3、6和79份材料;主坐标分析将材料分为5个亚群,分别包括5、10、12、24和26份材料。群体遗传结构分析将材料分为5个亚群,分别包含24、18、6、22、29份材料。 相似文献
6.
分析大豆亲本材料的遗传多样性,发掘农艺性状关联位点的优异等位变异,为大豆杂交组合的配置及分子标记辅助育种提供依据。本试验利用59个SSR标记对90份大豆种质资源进行多态性扫描和遗传多样性分析。筛选出46个标记,应用STRUCTURE2.3.2软件进行群体结构遗传分析,利用Tassel2.1软件MLM模型对18个农艺性状进行关联分析,发掘优异等位变异。结果表明:(1)59个标记中50个标记具有多态性,共检测出154个等位变异;多态性信息值PIC分布范围为0.042~0.765,平均PIC=0.421;SSR标记位点的Shannon指数(H’)的分布范围为0.1066~1.6804,平均值为0.8241;遗传距离的变异范围为0.0307~1.4529,平均值0.7015。(2)供试材料分为4个亚群。发现7个与分枝数、百粒重、叶形、主茎节数、生育期和蛋白含量相关联的标记,表型变异的解释率为0.003~0.339。研究在各关联位点找到了Satt263-A279、Satt156-A203、Satt567-A112等表型效应明显的优异等位变异。为大豆育种优异基因的克隆提供科学依据。 相似文献
7.
小麦RIL群体SSR标记偏分离的遗传分析 总被引：4，自引：0，他引：4
以普通小麦3338与斯卑尔脱小麦Altgold杂交得到的F6代重组近交系群体为材料，筛选出334个（271个SSR和63个EST-SSR标记）多态性标记，以此为基础构建了一个包含287个分子标记的遗传图谱。对这些多态性标记进行偏分离分析发现82个表现为偏分离（P＜0.05），其中70个可以定位到遗传连锁图谱中。这些偏分离标记中有33个标记位点偏向母本3338，占40.2%，49个标记位点偏向父本Altgold，占59.8%。这些偏分离标记在图谱上的分布有两种：成簇分布和孤立位点的偏分离。在5条不同染色体上发现6个偏分离热点区域，这些偏分离热点区域的形成可能与配子体选择有关。 相似文献
8.
基于SSR标记的梨资源遗传多样性分析 总被引：5，自引：1，他引：4
本研究利用SSR标记技术对56份梨资源进行了遗传多样性分析。利用筛选出的6对SSR引物共扩增40条谱带,其中多态性位点38个,多态性位点比例为95%,每对引物产生有效等位基因6.3个。各位点期望杂合度H值在0.0354～0.491,平均为0.1964;有效等位基因Ne值在1.0367～1.9648,平均值为1.2958;香农指数I值平均值为0.3256,说明了供试梨材料的遗传多样性较低。利用SSR标记可将44个栽培品种区分开,但无法区分芽变和原种。根据SSR标记揭示的多态性,采用NTSYS-pc软件,以UPGMA法进行聚类分析,结果显示所检测的56份梨材料在相似系数0.71处可分为4组,其中中国的白梨、秋子梨和砂梨相互交错在一起,没有独自各自成组。 相似文献
9.
菠菜转录组SSR位点分析及其分子标记的开发 总被引：1，自引：0，他引：1
简单重复序列(simple sequence repeat,SSR)分子标记具有多态性高、重复性好、共显性等特点,己广泛用于蔬菜作物遗传育种研究中.本研究利用MISA软件对菠菜(Spinacia oleracea)转录组SSR位点信息进行分析,开发适用于菠菜的SSR分子标记,并对不同菠菜材料的遗传多样性进行分析.结果显示,在获得的44 796条基因簇(Unigene)中检测出2 045个SSR位点,检测出2 045个SSR位点,分布在1 940条Unigene上,发生频率为4.33％,平均距离为19.81 kb.菠菜SSR中以单、三、四核苷酸重复为主,分别占总SSR的19％、65％和7％,重复长度主要为18～24 bp,占菠菜总SSR的93.55％,以重复次数6次和7次为主,分别占菠菜总SSR的31.42％和31.05％.菠菜转录组中单核苷酸重复类型为A/T,二核苷酸重复主要为GA/TC和AG/CT,三核苷酸重复主要以CAA/TTG、GTT/AAC、GAA/TTC、TGT/ACA和GAT/ATC为主.本研究共设计了2 199对SSR特异位点引物,随机挑选218对引物对9个不同类型的菠菜材料进行扩增检测,其中38对表现出多态性.利用UPGMA做图进行聚类分析,9份菠菜材料聚为3类.本研究通过对菠菜转录组SSR位点信息分析及其分子标记的开发,获得了多态性较为丰富的SSR位点,为菠菜遗传多样性的分析、遗传图谱的构建及分子标记辅助育种提供了基础资料. 相似文献
10.
SSR和SRAP标记研究油菜杂交种骨干亲本的遗传多样性 总被引：9，自引：2，他引：7
用SSR和SRAP两种分子标记方法研究51份甘蓝型油菜杂交种亲本系的遗传多样性,并对两种分子标记研究结果进行比较。结果发现,在51份材料中,45对SSR引物共扩增出194条多态性条带,平均每对引物为4.3条,25对SRAP引物共扩增出197条多态性条带,平均每对引物为7.9条。UPGMA聚类分析表明,SSR和SRAP标记都可将51份亲本材料划分为五大类群,本所选育的玻里马细胞质雄性不育系（Polima CMS）的主要保持系和恢复系都聚在同一类群的不同亚群中。根据系谱资料分析发现,SRAP标记划分的类群与系谱资料更为接近,SRAP标记更适用于遗传关系较近材料的遗传多样性分析。SRAP标记揭示的亲本间遗传距离要大于SSR标记揭示的遗传距离。两种不同标记方法揭示出油菜亲本遗传多样性的差异主要是由不同的标记方法揭示的标记位点等位基因变异数不同造成的。 相似文献
11.
低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响 总被引：2，自引：2，他引：0
【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。 相似文献
12.
Aleš Lebeda Ivana Doležalová Eva Křístková Barbora Mieslerová 《Genetic Resources and Crop Evolution》2001,48(2):153-164
Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions. 相似文献
13.
Kyle Devey Michael Mucalo Gordon Rajendram Joseph Lane 《Communications in Soil Science and Plant Analysis》2015,46(4):72-80
Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing. 相似文献
14.
Thomas Larsen Jesper Luxhøi Jakob Magid Lars Stoumann Jensen Paul Henning Krogh 《Biology and Fertility of Soils》2007,44(1):59-68
We have studied the effect of anaerobically digested (ADMSW) and composted municipal solid waste (CMSW) on mineralization
and foodweb dynamics to verify the hypothesis that ADMSW would immobilize N right after addition to soil in contrast to addition
with CMSW. Another hypothesis was that the mesofauna (enchytraeids and microarthropods) would stimulate N release from the
decomposer community. We measured excretion of -N and urea-N from the mesofauna and hypothesized that enchytraeids would release urea. ADMSW and CMSW were amended to pots
with sandy loam and barley. The pots were divided into treatments with or without mesofauna. Mesofauna, plant N and biomass,
soil N and ergosterol (fungal biomass) were measured over a 113-day period of four equidistant samplings. Soil respiration,
N mineralization and N release by the mesofauna were modelled from concurrent studies. ADMSW- and CMSW-treated soils initially
(<20 days) immobilized N. The amendments did not increase plant growth substantially, and this was probably due to N-limitation
in the early stages of plant growth. Enchytraeid abundance was about three times higher in ADMSW- than CMSW-treated soils,
indicating that ADMSW contained more labile compounds, bacteria, and microfauna. The mesofauna did not affect N-content, but
the cumulated -N excreted by the mesofauna was estimated to be substantial and about one-fifth of total plant N in ADMSW. An explanation
for this discrepancy might be that in the absence of the mesofauna, other members of the detrivore and microbivore community
performed the mesofauna’s function. Neither enchytraeids nor microarthropods excreted urea. 相似文献
15.
《中国生态农业学报》2014,(6)
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology, 相似文献
16.
《中国生态农业学报》2014,(7)
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology, 相似文献
17.
《中国生态农业学报》2014,(8)
正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology, 相似文献
18.
I.L. Boyd 《Biological conservation》2010,143(7):1664-1674
“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options. 相似文献
19.
Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage 总被引：1，自引：0，他引：1
Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate. 相似文献
20.
M.A. Callaham Jr. J.M. BlairT.C. Todd D.J. KitchenM.R. Whiles 《Soil biology & biochemistry》2003,35(8):1079-1093
The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils. 相似文献