共查询到19条相似文献，搜索用时 62 毫秒
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本文介绍2007年和2008年的12月下旬,选用捕自台湾海峡的野生牙鲆（Paralichthys olivaceus）（2～6kg／尾）作为亲鱼,约经45d左右人工驯养、促熟达到性成熟,在产卵池中自然产卵受精。经过一系列鱼苗培育操作过程,获得健康的苗种。 相似文献
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随着牙鲆养殖的产业化发展，牙鲆苗种生产逐渐由春季转向秋季，而秋季牙鲆苗种生产的关键技术是调控亲鱼秋季自然产卵。本文就调控牙鲆秋季产卵的基本原理与手法，简要阐述如下，仅供参考。一、调控牙鲆秋季性腺成熟、产卵的基本原理。鱼类性腺发育、成熟产卵必须在各自特定的环境条件下完成，尤以光照和温度起着最明显的调节作用，而营养和水质条件等也起着积极的作用。１．光照光照的长短影响许多鱼类的性腺发育、成熟产卵，性腺的周期性变化是受环境和季节变化的影响，这种季节变化中的主要刺激因素是光照。春夏产卵鱼类，卵母细胞的营养… 相似文献
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2011-2013年,利用野生和经人工驯养的野生斑鳜（ Siniperca scherzeri ）亲鱼进行人工繁殖试验。结果表明：采用地欧酮（ DOM）和促黄体素释放激素类似物（ LHRH -A3）组成的混合药物催产,在17．8-25．4℃水温条件下,斑鳜亲鱼群体的效应时间为24-46．5 h,存在产卵高峰期,产卵持续时间一般长达1-1．5 d。自然产卵高峰期的受精率较高,最高可达80%以上,早期和晚期的较低;而孵化率差异不大,最高可达86．7%。人工授精的受精率可达91．3%,孵化率可达89．3%。未经人工驯养的野生斑鳜亲鱼人工催产的受精率、孵化率都明显低于经人工驯养的。 相似文献
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本文用2种不同培育方式对台湾海峡野生牙鲆亲鱼培育与促熟的效果进行了比较。结果表明,土池培养的亲鱼移入土池网箱中培育促熟,当性腺发育成熟时再从土池网箱移到室内水泥池产卵是亲鱼促熟产卵的较佳方式。 相似文献
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漠斑牙鲆人工育苗技术要点 总被引：12，自引：0，他引：12
漠斑牙鲆(Paralichthys lethostigma)原产于美国沿海,是一种优质的水产养殖品种。在美国,漠斑牙鲆的亲鱼培育、产卵、育苗等基础研究已进展多年;近几年来,国内先后有4个-5个单位从美国引进了漠斑牙鲆亲鱼或鱼苗进行培育,有的单位已取得了一定的进展,有的单位还遇到了不少技术难题,亲鱼培育与人工繁殖技术还未过关。2003年春季,笔者在美国佛罗里达州用20尾亲鱼进行催产、自然产卵,共获得卵黄苗100多 相似文献
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<正> 目前我国牙鲆苗种生产几乎完全依靠捕获天然亲鱼采卵孵化。本试验是将天然牙鲆亲鱼驯化越冬后使之自然产卵,为今后牙鲆的全人工苗种大量生产打下基础。一、天然牙鲆亲鱼越冬前的驯化 1.诱导摄食本试验所用牙鲆亲鱼是1990年5月18日～5月25日在秦皇岛海域用流刺网和袖网捕获13尾亲鱼中存活下来的9尾(♀6尾,全长48～70cm,体重1650～2950g;♂3尾,全长54～60cm,体重1850 相似文献
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舟山野生牙鲆Paralichthys olivaceus(Temminck et Sehlegel)的人工驯养试验 总被引：1，自引：0，他引：1
对不同渔获方式捕捞的舟山野生牙鲆进行不同方式的驯养试验.结果表明:(1)拖网捕获的活体野生牙鲆数量较少,在室内水泥池中可以驯养成活,但成活率较低,仅为54.5%,驯食的最佳饵料为活体脊尾白虾;(2)推缉网捕获的牙鲆幼鱼数量较多,全长8～15cm的幼鱼在池塘中养殖1年的成活率为46.2%,选择部分作为亲鱼培育,2周龄的成活率为86.4%,3周龄亲鱼养殖成活率达89.4%,目前拥有亲鱼3周龄鱼320尾、2周龄鱼60尾. 相似文献
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日前，由莱州市大华水产有限公司进行的“漠斑牙鲆子二代苗种繁育与养殖技术研究”通过了省科技厅组织的专家鉴定。该公司在2002年以来进行漠斑牙鲆引进与苗种规模化生产研究和漠斑牙鲆养殖研究的基础上，于2004年以来开展漠斑牙鲆子二代苗种繁育与养殖技术研究，通过4年多的试验研究和生产实践，建立了漠斑牙鲆子二代苗种人工繁育、养殖工艺技术和养殖模式，实现了亲鱼自然产卵，验证了漠斑牙鲆适合于池塘养殖、工厂化养殖和网箱养殖。共培育出性状优良的漠斑牙鲆子一代亲鱼200尾，繁育子二代苗种94．3万尾，延续了子一代鱼的优良性状。试验养殖，池塘养殖成活率达到90％，每667m^2产量达到276．8kg；工厂化养殖成活率达到95％，单产达到每平方米11．7kg；网箱养殖成活率达到86．9％，单产达到每平方米10．4kg。养殖效益显著。 相似文献
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This study documents early out-of-season induced spawning of channel catfish Ictalurus punctatus. During the early spring (February–April) of 1999, 2000 and 2001, ponds containing (1) male and female channel catfish (mixed-sex ponds) or (2) male channel and blue catfish I. furcatus only, or female channel catfish only (single-sex ponds) were heated to 24–30°C to encourage gonadal maturation and spawning. Unheated ponds were stocked with males and females and were monitored during the duration of heating. When natural spawning occurred in the heated ponds, the fish were captured by seining and unspawned females were injected with 100 μg kg−1 of synthetic leutenizing hormone-releasing hormone. Injected females were either paired with males or held in communal all-female groups, and monitored for ovulation. Eggs were collected and fertilized with sperm of channel catfish or blue catfish. Females paired with males were induced to spawn 44 days (mixed-sex ponds) and 50 days (single-sex ponds) before natural spawning occurred in unheated ponds. Spawning latency (the time between injection and ovulation) and the percentage of neurulated embryos from eggs fertilized using channel catfish sperm was not different between spawning before the natural season (P=0.68) and during the natural season in fish from mixed-sex ponds (P=0.57). Females held in all-female groups produced eggs 34 days before the onset of spawning in unheated ponds. Spawning latency was not different between spawns before and during the natural season (P=0.16), and the percentages of neurulated embryos from eggs fertilized with channel catfish sperm (P=0.76) or blue catfish sperm (P=0.77) before or during the natural season were not different. This study demonstrates the feasibility of conditioning of channel catfish females for early out-of-season induced spawning in the laboratory. 相似文献
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《Journal Of Applied Aquaculture》2013,25(4):23-32
ABSTRACT Mature female Indian catfish, Heteropneustes fossilis were induced to spawn in spawning season (July-August) by various ovulation-inducing agents, and the frequency of ovulation was checked after a latency of 12-14 hours of the treatment. Egg mass and fertilization rate were taken as end-points of spawning performance. [D-Ala6-Pro9] ethylamide GnRH analogue in dosages of 0.075, 0.15, 0.2, and 0.5 μg/g of body weight resulted in 28.5%, 71%, 86%, and 86% ovulation, respectively, but the lower doses of 0.01 and 0.05 μg/g body weight failed to induce ovulation in early spawning phase (July). The dopamine-2 (DA2) receptor antagonists pimozide, domperidone, and metoclopramide potentiated the anovulatory dose of 0.05 μ g GnRH analogue to induce ovulation in 86%, 86%, and 50% of the females, respectively, in the early spawning phase (July). The DA precursor, L-dihydroxyphenylalanine (L-DOPA; 10 μg/g body weight) and the DA agonist bromocriptine (10 μg/g body weight) decreased markedly the ovulatory response of 0.2 μ g GnRH analogue to 29%. The catechol-amine-synthesis inhibitor a-methylparatyrosine produced a mild depressing effect on the ovulatory response of 0.2 μ g GnRH analogue. Egg mass was high in groups that yielded a high ovulatory response (71% and above) except the a-MPT groups. The fertilization rate, however, was not affected, irrespective of the ovulatory response. The results show that the Indian catfish can be induced to spawn by superac-tive GnRH analogue alone in a dosage range of 0.15-0.2 μg/g body weight or in combination with DA2 antagonists, such as pimozide or domperidone, in a very low dosage of 0.05 μg/g. 相似文献
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Marcelo Pimenta de Amorim Bruno Vilaça Campos Gomes Yuri Simões Martins Yoshimi Sato Elizete Rizzo & Nilo Bazzoli 《Aquaculture Research》2009,40(2):172-180
The silver catfish, Rhamdia quelen , is endemic to North, Central and South America with high aquaculture potential and wide acceptance in the market. Breeder fish were subjected to induced reproduction through hypophysation using a crude common carp pituitary extract. Egg characteristics, oocyte surface ultrastructure and histology of larval ontogenesis until whole yolk resorption were described for the first time for this species. Oocytes and semen were obtained by manual extrusion, and fertilization was conducted using the dry method. After fertilization, eggs were kept in incubators at 24 °C. The embryonic development was monitored using a stereomicroscope every 10 min until hatching. To analyse the larval development, larvae samples were collected from incubators daily until the fifth day, fixed in Bouin's fluid and subjected to routine histological techniques. The oocyte extrusion occurred 8 h after the second hormone dose at 26 °C. The oocytes were spherical, non-adhesive and yellow, with a diameter of 1471.75±47.63 μm. Scanning electron microscopy revealed a thin jelly coat covering the zona radiata in the animal pole around the micropyle. The blastopore closure occurred within 8 h after fertilization, and the fertilization rate was 79.9±5.2% at 24 °C. Embryonic development was completed within 25 h 30 min after fertilization. The complete resorption of the yolk and the formation of the digestive system organs and the mouth opening occurred on the fifth day, indicating a need for exogenous feeding. The results of this study provide information important for improvement in R. quelen culture and management. 相似文献
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中国蛤蜊的诱导产卵和胚胎发育 总被引：2，自引：0，他引：2
成熟期的亲贝经强化培育后,采用常规手段,进行人工诱导产卵,可获得大量的受精卵,并能正常分裂发育,显微观察并记录了胚胎发育过程和形态特征。 相似文献
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Aquaculture of Spinibarbus denticulatus (Oshima, 1926), a fish species indigenous to North Vietnam and Eastern China, is constrained by lack of fingerlings for stocking ponds and cages. As these fish do not naturally breed in captivity, carp pituitary extract (CPE), luteinizing hormone-releasing hormone analogue (LHRHa) with domperidone (DOM) and human chorionic gonadotropin (HCG) were administered at various doses to induce ovulation. A first set of experiments evaluated the response to LHRHa (30, 40 and 50 μg kg−1) with or without DOM (10 mg kg−1), CPE (20, 30 and 40 mg kg−1) and HCG (3000, 4000 and 5000 IU kg−1). A second set of experiments evaluated the dose response to LHRHa (30, 35, 40 and 50 μg kg−1) primed with 6 mg kg−1 of CPE, and HCG (3000, 3500, 4000, 5000 IU kg−1) primed with 6 mg kg−1 of CPE. The treatment groups were compared with each other and the control (injected with 0.9% saline solution). Only 25% and 50% ovulation resulted when treated with LHRHa at 40 and 50 μg kg−1, whereas 100% ovulation was achieved with an addition of DOM to LHRHa. Both 30 and 40 mg kg−1 CPE induced 100% ovulation. However, HCG (4000 and 5000 IU kg−1) induced ovulation in only 33% of females. When primed with CPE, the minimum dose of LHRHa required was 35 μg kg−1 to achieve 70% ovulation. Priming HCG with CPE also resulted in 100% ovulation, and the minimum effective dose of HCG to induce ovulation was 3500 IU kg−1 with 60% ovulation. Fertilization and hatch rates observed in this study with different hormonal stimulation were high (80–93%). The results indicate that while the use of combined hormone strategy has no apparent advantage over a single hormone strategy, LHRHa+DOM (40 μg kg−1+10.0 mg kg−1) and CPE (30 mg kg−1) are most effective in consistently inducing ovulation and thus can be used for commercial hatchery production of S. denticulatus larvae. 相似文献
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The aim of this study was to induce and synchronize spawning of pejerrey Odontesthes bonariensis (Valenciennes, 1835), using gonadotropin releasing hormone agonist (GnRHa) implants. In the first experiment, the ovarian condition was assessed by ovarian biopsies and the measurement of the genital pore width (GPW). Females having the leading clutch of oocytes with a diameter of around 800–900 μm and a GPW between 4.5 and 5.5 mm were treated with GnRHa implants. Eighty per cent of females spawned between 2 and 9 days after treatment, 12 days earlier than 20% of the fish in the control group that presented signs of spawning activity. In order to avoid any possible ovarian injury and/or stress by the catheterization procedure, in a second experiment, females were selected only by visual inspection of the abdomen and GPW measurement. As in experiment 1, 80% of females spawned between 2 and 8 days after treatment, 8 days earlier than 30% of the fish that spawned in the control group. In both experiments, fertilization and hatching success were similar between control and GnRHa‐treated groups. These results clearly demonstrated that GnRHa implantation can advance and synchronize ovulation and spawning in pejerrey without affecting egg quality. 相似文献
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Sexually mature female hatchery‐reared snapper, Pagrus auratus (Bloch & Schneider) were captured from sea cages by handline and injected at first capture (control) or 24 h after capture, transport and subsequent confinement (delayed injection) with either saline, luteinizing hormone releasing hormone analogue, human chorionic gonadotropin, or 17α‐hydroxyprogesterone. Blood was sampled before hormone treatment and again after 168 h, and fish were checked daily for ovulation. Plasma levels of 17β‐estradiol (E2), testosterone (T), 17α, 20β dihydroxy‐4‐pregnen‐3‐one (17, 20βP) and cortisol were determined by radioimmunoassay. The ovulatory response was assessed from the proportion of fish ovulating, ovulation volume, egg quality and fertility. A delay in injection resulted in significantly lower plasma E2 and T levels in response to hormone treatment, smaller ovulation volumes, and poorer egg quality than in control fish. The results are consistent with the generally inhibitory effects of stress on reproduction in fish, and confirm the requirement to treat fish with hormones designed to induce ovulation, as soon as possible after capture and disturbance. 相似文献
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Artemia-mediated delivery of a gonadotropin- releasing hormone analogue (GnRHa) to broodstock cardinal tetras showed the potential to induce ovulation, as assessed by manual stripping. Exposure of the Artemia to the GnRHa for between 30 and 60 min was optimal for inducing ovulation while shorter and longer periods showed a trend to a decreasing percentage of ovulating fish. Further research on this method is warranted as the method could be applied to other fish species. 相似文献
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Triploid induction in Australian greenlip abalone, Haliotis laevigata (Donovan), was conducted by blocking the formation of the second polar body using cytochalasin B (CB). Twenty minutes after fertilization, the zygotes of greenlip abalone were treated with four CB concentrations (0, 0.25, 0.5 and 0.75 mg L−1) for 10, 15 and 20 min. The ploidy of resultant larvae was determined using flow cytometry at 72-h post fertilization. Our study showed that fertilization, hatching, survival and induced triploidy of abalone larvae were significantly affected by the CB concentration and treatment duration. The effective range of CB concentration for triploid induction on greenlip abalone was 0.5–0.75 mg L−1 with an induction duration of 10–15 min. The results indicate that the most effective treatment combination for triploid induction in greenlip abalone is 0.5 mg CB L−1 for 15 min starting at 20-min post fertilization. 相似文献