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探讨并优化了测定苯酚-硫酸法灵芝(Ganoderma lucidum)孢子粉多糖含量的检测条件,通过单因素和正交试验方法确定灵芝孢子粉多糖的最佳检测条件,并分析不同检测方法测定灵芝孢子粉多糖含量的差异。结果表明,灵芝孢子粉多糖苯酚-硫酸法的最佳测定条件为最大吸收波长489 nm,5%苯酚用量0.5 m L,浓硫酸用量2.5 m L,提取温度90℃,提取时间3.0 h,料液比(g·m L~(-1))为1:40,在此条件下平均回收率为99.54%,相对标准偏差(RSD)为0.90%。本试验优化后的检测方法具有良好的稳定性及重复性,回收率高,并能有效减少检测试剂苯酚和浓硫酸的使用量,具有实际应用意义。 相似文献
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以16批（S1～S16）不同来源的灵芝（Ganoderma lucidum）孢子粉为材料，构建灵芝孢子粉脂质成分高效液相色谱-蒸发光散射检测（HPLC-ELSD）指纹图谱，将S1～S16的HPLC-ELSD指纹图谱数据导入中药色谱指纹图谱相似度评价系统（2012版），指认共有峰和评价相似度，采用HPLC-ELSD测定S1～S16中亚油酸、棕榈酸、油酸和麦角甾醇含量。结果表明：共指认4个共有峰，分别为亚油酸、棕榈酸、油酸和麦角甾醇；除S14外，其他15批灵芝孢子粉中脂质成分HPLC-ELSD指纹图谱相似度高，较为稳定；不同来源的灵芝孢子粉中亚油酸、棕榈酸和油酸的含量差异较大，麦角甾醇含量较为稳定。 相似文献
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破壁灵芝孢子粉的制备及体外抗氧化活性研究 总被引：1，自引：0，他引：1
超音速气流破壁制备破壁灵芝孢子粉;对破壁与未破壁灵芝孢子粉的醇提物和水提物,分别利用DPPH、ABTS、FRAP、羟自由基的清除能力和超氧阴离子清除能力,测定抗氧化活性。灵芝孢子粉破壁率达到98%以上;灵芝孢子醇提物的抗氧化活性:E1(破壁灵芝孢子粉95%乙醇提取)活性都较高;DPPH、ABTS和羟自由基清除率的EC50较E0(未破壁灵芝孢子粉95%乙醇提取)分别低46.53%、77.11%和69.51%,FRAP值和ORAC值分别高出287.87%和30.18%。灵芝孢子水提物的抗氧化活性:W0(未破壁灵芝孢子水提物)活性都为最低,其他4种水提物抗氧化能力相似。破壁之后的灵芝孢子粉抗氧化活性比未破壁灵芝孢子粉高;但破壁灵芝孢子由于直接接触空气,因此,放置时其活性物质极易变质。 相似文献
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《中国果菜》2020,(6)
本研究考察了破壁灵芝孢子粉储藏过程中不同储藏温度、光照环境以及真空条件对其色泽、酸价、过氧化值的影响,以期确定适宜的储藏条件,为破壁灵芝孢子粉的产业化储藏提供指导。结果表明,在相同储藏条件下,随着储藏温度的升高,孢子粉变亮变红趋势逐渐加剧,40℃高温会加速该色泽的变化,而不同光照和真空条件对该色泽变化影响相对较小;随着储藏温度的升高,孢子粉酸败过程越快,在35 d时,40℃较-18℃酸价值增大20.14%,抽真空包装可延缓其酸败过程,光照时间长短对酸价影响相对较小;随着储藏温度的升高和光照时间的延长,孢子粉氧化过程加快,在35 d时,40℃条件下过氧化值较-18℃增加29.52%,而全光条件较避光增加45.62%。在35 d时,抽真空较不抽真空过氧化值降低21.27%。综上所述,避光、抽真空及-18℃低温储藏条件,更利于破壁灵芝孢子粉色泽的保持、酸败及氧化进程的减缓。 相似文献
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WANG Wei-qun WANG Shu-qiu LIU Yue-xia MA Xiao-ru YU Hai-bo WANG Bai-xin MENG De-xin ZHANG Ming-liang 《园艺学报》2007,23(6):1149-1152
AIM: To observe the influence of Ganoderma lucidum spores on the cytokine IL-1β and c-Fos in the brain tissue of epilepsy rats. METHODS: The IL-1β level was examined by radioimmunoassay and the c-Fos expression was measured by mmunohistochemical assay. RESULTS: Comparing the Ganoderma lucidum spores group with the epilepsy model group: c-Fos positive cell count in brain cortex and hippocampus in treatment group was obviously reduced. IL-1β level in brain tissue was also reduced obviously. CONCLUSION: Ganoderma lucidum spores effectively reduces cytokine IL-1β in brain tissue of epilepsy rats, improves the immunity dysfunction and plays a role in anti-epilepsy through suppressing c-Fos expression in epilepsy rats brain tissue and blocking of LRG. 相似文献
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对不同生长期（接种前、出芝前、出芝后、发酵后）菌草灵芝培养基的常规营养成分及Ca、P含量进行了测定与分析,并且对出芝菌糟的粗多糖、氨基酸和重金属含量进行了测定。结果表明,灵芝菌糟营养成分含量丰富,富舍粗多糖、粗蛋白、粗脂肪、矿质元素、氨基酸等营养成分。重金属含量在饲料卫生标准规定的范围内,且远低于有机肥料的标准。饲用价值大小排序为发酵菌糟〉出芝菌糟〉未出芝培养料〉培养基质。菌草灵芝菌糟在用作饲料及饲料添加剂、肥料、无土栽培基质等方面具有很大的开发潜力。 相似文献
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ZHAO Shuang KANG Yu-ming ZHANG Sheng-chang WANG Shu-qiu WANG Shao-qing MA Xiao-ru ZHANG Ting 《园艺学报》2007,23(6):1153-1156
AIM: To investigate the effect of Ganoderma lucidum spores powder on the expression of insulin-like growth factor-1 (IGF-1), nuclear factor-κB (NF-κB) and apoptosis of nerve cells in rats with epilepsy established by pentetrazole. METHODS: The sub-eclampsia dosage of pentetrazole (PTZ) was used to make epilepsy model. Ganoderma lucidum spores powder group was given from stomach. The enduring time and latent period were recorded. The immune reactivity of IGF-1, NF-κB/P65 and apoptosis of nerve cells were measured with immunohistochemical staining and TUNEL method. RESULTS: In high power sight (×400), there were much more apoptosis cells in hippocampus and brain cortex of model group (18.80±2.13, 16.87±2.00) than those in control group (0.97±0.52, 0.58±0.25). The expressions of IGF-1, NF-κB in model group were higher than those in control group. Compared with model group, the latent period of Ganoderma lucidum spores powder group at the 17th, 21th, 25th days were longer (P<0.05, P<0.05, P<0.01, respectively).There were less apoptosis cells in hippocampus and brain cortex of Ganoderma lucidum spores powder group (12.30±2.46, 10.48±1.33) than those in model group. The expression of NF-κB/P65 in Ganoderma lucidum spores powder group was lower than that in model group, but the immune reactivity of IGF-1 increased more distinctly in Ganoderma lucidum spores powder group than that in model group. CONCLUSION: IGF-1, NF-κB and apoptosis of nerve cells may have play a role in occurrence and development of PTZ-induced epilepsy. Ganoderma lucidum spores powder can suppress expression of NF-κB strongly, and facilitate the immune reactivity of IGF-1, which may be one of the mechanisms by which Ganoderma lucidum spores powder restrains the apoptosis of nerve cells caused by epilepsy to prevent the damages of nerve cells. 相似文献
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对灵芝(Ganoderma lucidum)孢子进行热水提取和超临界CO2萃取,将得到提取物和萃取物按一定比例调制成试验样品进行延缓皮肤衰老功效的研究.感官评估结果显示,受试者连续使用试验样品12周后,在皮肤干燥度、弹性、光泽度、细小皱纹和光滑度达到中等改善的比例分别为25.8％、6.5％、25.8％、6.4％和29％;专业仪器测定结果显示,第8周开始,皮肤弹性明显改善(P＜0.05);第12周,皮肤粗糙度明显的改善(P＜0.05).表明灵芝孢子活性成分具有一定的延缓皮肤衰老作用. 相似文献
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原木灵芝孢子研究：（Ⅲ）与木屑袋栽灵芝孢子的鉴别 总被引：1，自引：1，他引：0
原木灵芝孢子与木屑袋栽灵芝孢子可以鉴别如下:1.在扫描电镜下,前者形态饱满,后者大多数孢壁内陷;2.X-射线能谱面扫描分析结果表明,两者Mg、Al、Si、P、S、Cl、K、Ca、Fe、Ni等无机元素相对含量差异显著,前者Si、Ca含量较高;3.高效液相色谱分析结果表明,前者灵芝酸(Ganoderic acid)A、B等灵芝三萜酸含量较高,而后者未检出Ganoderic acid C;4.孢子粉经乙醚脱脂后用乙醇回流提取、浓缩成浸膏,前者提取率(固形物含量)达4.2％～4.8％,而后者仅为1.9％～2.1％。 相似文献
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灵芝孢子的综合破壁技术专利是公认的无形资产,,对灵芝加工技术科技创新和灵芝产业发展具有重要的作用,应加以重点保护。通过对相关的专利知识产权保护法律法规进行总结分析,详细讨论了灵芝孢子破壁技术的专利维权问题,提出了具体的专利保护措施和建议。加强专利保护,可以为灵芝孢子生产和整个灵芝产业的健康发展保驾护航,保持其市场竞争优势。 相似文献
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WANG Shu-qiu LI Xiao-jie JIANG Zhi-mei QIN Xiao-yu HAN Yu-ze LIU Lei LIU Jun-xing WANG Fang-fang LIANG Yan-feng WANG Shu-xiang 《园艺学报》2011,27(6):1053-1058
AIM: To investigate the effects of Ganoderma lucidum spores on superoxide dismutase(SOD),malondialdehyde(MDA),total antioxidative capacity(T-AOC), cytochrome C, heat-shock protein 70 (HSP70), mitochondrial Ca2+ and brain-derived neurotrophic factor(BDNF) in the brain tissues of epilepsy rats.METHODS: The rat chronic epilepsy model was by intraperitoneal injection of pentetrazole(PTZ) at a subconvulsant dose (32 mg/kg).Flame atomic absorption method was used to detect the content of mitochondrial Ca2+,and spectrophotometer colorimetry was used to measure SOD activity,MDA content,T-AOC and cytochrome C levels in rat brain tissues. HSP70 and BDNF were determined by immunohistochemical method.RESULTS: The contents of mitochondrial Ca2+ and cytochrome C were higher, and the content of intracytoplasmic cytochrome C in the rat brain tissues was obviously lower in Ganoderma lucidum spores group than that in epileptic model group. Compared to epileptic model group, the activity of SOD and T-AOC in cytoplasm of the rat brain tissues decreased while MDA increased, and the numbers of BDNF-positive cells in cerebral cortex and hippocampus were significantly increased in Ganoderma lucidum spores group. The positive neuron population of HSP70 in hippocampus, basal nucleus and cortex was significantly higher in Ganoderma lucidum spores group than that in epilepsy model group.CONCLUSION: Ganoderma lucidum spores attenuate the impairment of neuronal mitochondria induced by seizure, and accelerate the expression of BDNF, resulting in restoring the energy metabolism in mitochondrion, thus alleviating the impairment and apoptosis of the brain tissues. 相似文献