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以四川省7个市县地区14个猪场为研究对象,采集疑似圆环病毒2型感染的死猪、病料、精液、血样等样品181份,进行PCR检测。结果显示:猪圆环病毒2型(PCV2)阳性有89份,阳性率49.2%;14个规模化猪场中均检测出了PCV2阳性。在死亡的43头猪中有21头PCV2阳性。病料中PCV2的阳性检出率54.0%。该病在春夏季节感染率最高,并存在病毒混合感染和继发细菌感染情况。根据临床症状和病理变化分析,大部分猪场因感染PCV2而致断奶仔猪多系统衰竭综合征(PMWS),其中遂宁市和绵阳市的6个规模化猪场中检测到PCV2感染所引起的猪皮炎肾病综合征(PDNS)。结果表明,四川省部分地区的规模化猪场中已经广泛存在猪圆环病毒2型感染。 相似文献
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《畜牧兽医科技信息》2018,(11)
猪圆环病毒2型(Porcine circovirus type2 infection,PCV2)是引起断奶仔猪多系统衰竭综合征(PMWS)、猪皮炎-肾病综合征(PDNS)、繁殖障碍性疾病、肺炎、肠炎、先天性震颤等一系列症候群的病原。可导致规模猪场遭受严重的病死经济损失。对近年规模猪场感染PCV2的变化及临床特点进行深入研究,总结出更为实用高效的综合治疗方案,有效降低规模猪场PCV2的感染发病风险及病死率,保障了规模养猪的经济效益。 相似文献
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猪Ⅱ型圆环病毒（PCV2）感染可引起几种不同的临床症状和疾病,包括断奶仔猪多系统衰竭综合征（PMWS）、猪皮炎肾病综合征（PDNS）、繁殖障碍、猪呼吸道综合征（PBDC）、增生性肠炎、坏死性淋巴腺炎和渗出性皮炎,关于出生仔猪先天性震颤是否由PCV2引起的还有很大的争议。除PMWS外,圆环病毒引起的其它疾病人们知之甚少,本文重点介绍PCV2引起的其它疾病。 相似文献
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正猪圆环病毒(PCV)在驻马店猪场感染十分普遍,据2016～2017年驻马店市动物疫病预防控制中心对辖区内猪场血清学调查发现圆环病毒2型(PCV-2)感染率高达77.34%。PCV能引起猪的多种发病症状,如断奶仔猪多系统衰竭综合征(PMWS)、猪皮炎肾病综合征(PMWS)、猪呼吸道综合征(PRDC)、仔猪先天性震颤(CT)等。现将一例保育猪发生圆环病毒感染的病例诊治分析如下。 相似文献
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张聪华 《畜牧兽医科技信息》2011,(6)
春夏之交,气候多变,应激增加,圆环病毒病在猪场整体预防上又变得异常重要,规模化猪场要做好攻坚预防必须对本病要有一个深入的了解.圆环病毒(PCV2)可引起猪的传染性、先天性颤抖和仔猪多系统衰弱综合症(PMWS)、猪皮炎和肾病综合症(PDNS)、呼吸道综合症(PRDC)繁殖障碍、肠炎与先天性颤抖等病,根据笔者的调查,在浙江猪感染圆环病后主要流行仔猪多系统衰弱综合症、猪皮炎和肾病综合症和呼吸道综合为主,规模猪场在同一个猪场内可以同时出现三种情况,并且往往出现繁殖障碍. 相似文献
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Jemersić L Cvetnić Z Toplak I Spicić S Grom J Barlic-Maganja D Terzić S Hostnik P Lojkić M Humski A Habrun B Krt B 《Research in veterinary science》2004,77(2):171-175
Porcine circovirus type 2 (PCV2) from the Circoviridae family has recently been associated with two serious diseases of swine, post-weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). During 2002, several outbreaks of clinical disease in pigs with weights ranging from 10 to 70 kg occurred on four farms in different locations in Croatia. The signs were consistent with PMWS and PDNS. Apart from progressive weight loss, pneumonia and/or diarrhoea, multifocal erythematous skin lesions and dermal necrosis were also observed. The PCR results obtained from PCV2 specific oligonucleotide primers confirmed a PCV2 infection. In addition, archive samples that were classical swine fever virus positive and derived from domestic pigs during an outbreak in 1997 were included in this study and one out of the three isolates was found to be positive for PCV2. For a better epizootiological understanding, genetic typing of representative isolates was carried out and compared with available isolates reported in the GenBank databases. 相似文献
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The detection of porcine circovirus in the Australian pig herd 总被引：4，自引：0，他引：4
Raye W Muhling J Warfe L Buddle JR Palmer C Wilcox GE 《Australian veterinary journal》2005,83(5):300-304
OBJECTIVE: To determine if porcine circovirus (PCV) type 1 (PCV1) or type 2 (PCV2) is present in the Australian pig herd, to conduct preliminary genetic characterisation of any viruses detected, and to determine if there is any obvious virological reason why post-weaning multisystemic wasting disease (PMWS), associated with PCV infection in other countries, has not been detected in Australia. DESIGN: Serum samples were collected from 14 randomly selected pig farms in Western Australia and used for detection of PCV antibody. Additional samples from one farm were obtained at 2-week intervals from pigs between 2 and 12 weeks of age to detect any age-associated variations in prevalence of infection. Veterinary practitioners from four Australian states submitted tissues of dead or unthrifty weaned pigs, and these were examined for evidence of PCV1 and PCV2 infection. PROCEDURE: Sera were tested for antibody to PCV using an indirect immunofluorescence assay (IFA). Tissues were tested for PCV1 and PCV2 genomic material using a multiplex PCR. RESULTS: PCV antibody was detected in approximately 30% of Western Australian pigs tested. PCV1 DNA was detected in tissue samples from Western Australia, South Australia and New South Wales and PCV2 DNA was detected in tissue samples from Western Australia, New South Wales and Queensland. Sequence analysis of the PCR products indicated the PCV1 and PCV2 present in Australia were very similar to strains in other countries where PMWS is endemic. CONCLUSION: Both PCV1 and PCV2 are present in Australia and the viruses present appear similar to those in countries with PMWS. The absence of PCV2-associated PMWS in Australia may be due to absence of essential secondary factors required for PCV2 to produce PMWS. 相似文献
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The presence of co-infections in pigs with clinical signs of PMWS in The Netherlands: a case-control study 总被引：8，自引：0，他引：8
Wellenberg GJ Stockhofe-Zurwieden N Boersma WJ De Jong MF Elbers AR 《Research in veterinary science》2004,77(2):177-184
In this study, 60 pigs with clinical signs of post-weaning multisystemic wasting syndrome (PMWS) from 20 different pig herds and 180 control pigs (without clinical signs of PMWS) were examined to get more insights into the frequencies of porcine circovirus 2 infections and the presence of co-infections in pigs with and without clinical signs of PMWS in the Netherlands. Porcine circovirus type 2 was detected in 100% of the pigs with clinical signs of PMWS by virus isolation and/or PCR and in 50% of the pigs from PMWS-free herds. There was an association between the levels of infectious PCV2 and/or PCV2 DNA load and the severity of clinical signs as described for PMWS. A high variation in PCV2 antibody titres was found in the clinically affected pigs, and 27% of these pigs did not mount PCV2 antibody titres higher than 1:200. A concurrent infection of PCV2 and porcine reproductive and respiratory syndrome virus (PRRSV) was found in at least 83% of the pigs with clinical signs of PMWS and in 35% of the pigs from PMWS-free herds. Co-infections of European- and American-type PRRSV were detected only in PMWS herds and in one control herd with a history of PMWS clinical signs. 相似文献
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为了解吉林地区猪细环病毒(TTSuVs)与II型猪圆环病毒(PCV2)混合感染情况,分析TTSuVs感染与仔猪多系统衰竭征(PMWS)的相关性。试验通过PCR技术对2019年收集到的吉林部分地区的10家规模化猪场130份血清样本进行检测;并应用本实验已建立的PCV2、TTSuVs实时荧光定量PCR检测方法,对PCV2阳性样品进行病毒载量的分析,区分PMWS病猪与PCV2亚临床感染猪,比较PMWS病猪与PCV2亚临床感染猪中TTSuVs的载量。结果显示:TTSuV1感染率为38.46%;TTSuV2感染率为57.69%;PCV2感染率为46.15%;TTSuV1与TTSuV2的混合感染率为31.54%;TTSuV1与PCV2的混合感染率为32.30%;TTSuV2与PCV2的混合感染率为44.62%;三种病毒的混合感染率为22.30%。且PMWS病猪血清中TTSuV2载量明显高于PCV2亚临床感染猪(P<0.01)。结果表明,TTSuV2载量与PMWS病的发生存在一定程度的相关性。 相似文献
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Saoulidis K Kyriakis SC Kennedy S Lekkas S Miliotis ChC Allan G Balkamos GC Papoutsis PA 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(4):202-205
Post-weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS) are two recently described conditions of pigs at the late nursery and fattening stages. The aim of this short communication was to describe the first reported occurrence of these conditions and of porcine circovirus type 2 (PCV2) infection in Greece. The clinical signs, gross post-mortem changes and histopathological changes observed in affected pigs, were similar to those previously described for both PDNS and PMWS. As in previous reports, the lesions were associated with PCV2 infection, which was demonstrated by immunohistochemical and in situ hybridization methods. 相似文献
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K. SAOULIDIS S. C. KYRIAKIS S. KENNEDY S. LEKKAS CH. C. MILIOTIS G. ALLAN G. C. BALKAMOS P. A. PAPOUTSIS 《Zoonoses and public health》2002,49(4):202-205
Post‐weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS) are two recently described conditions of pigs at the late nursery and fattening stages. The aim of this short communication was to describe the first reported occurrence of these conditions and of porcine circovirus type 2 (PCV2) infection in Greece. The clinical signs, gross post‐mortem changes and histopathological changes observed in affected pigs, were similar to those previously described for both PDNS and PMWS. As in previous reports, the lesions were associated with PCV2 infection, which was demonstrated by immunohistochemical and in situ hybridization methods. 相似文献
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OBJECTIVE: To determine if postweaning multisystemic wasting syndrome (PMWS) is occurring in the New South Wales pig population and to determine the current and past seroprevalence of porcine circovirus 2 (PCV2). DESIGN: Pig veterinarians were contacted seeking submission of tissues from animals with clinical signs suggestive of PMWS. Samples were also accepted from suspected cases of porcine dermatitis and nephropathy syndrome (PDNS). Serological studies were also undertaken on archival sera and sera submitted during the study. PROCEDURE: Histopathological examination was undertaken on all tissues submitted. The presence of PCV2 was determined by immunohistochemistry. Sera were tested for PCV2 using a commercial enzyme linked immunosorbent assay kit modified for testing of serum samples. RESULTS: No cases of PMWS were identified during the study. Four cases of PDNS were identified. PCV2 antibody was detected in 80% of archival sera from 1995 and 75.8% from 2001. Seroprevalence in samples tested during 2002-2003 was 87.8%. PCV2 was isolated from tissues of a case of PDNS. CONCLUSION: PCV2 is widespread in the New South Wales pig population and has been since at least 1995. This study describes the first isolation of an Australian PCV2. No cases of PMWS were identified in New South Wales. 相似文献
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Porcine circovirus diseases 总被引：21，自引：0，他引：21
Segalés J Allan GM Domingo M 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2005,6(2):119-142
Porcine circovirus type 2 (PCV2) is a member of the family Circoviridae, a recently established virus family composed of small, non-enveloped viruses, with a circular, single-stranded DNA genome. PCV2, which is found all over the world in the domestic pig and probably the wild boar, has been recently associated with a number of disease syndromes, which have been collectively named porcine circovirus diseases (PCVD). Postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis and nephropathy syndrome (PDNS) and reproductive disorders are the most relevant ones. Among them, only PMWS is considered to have a severe impact on domestic swine production. PMWS mainly affects nursery and/or fattening pigs; wasting is considered the most representative clinical sign in this disease. Diagnosis of this disease is confirmed by histopathological examination of lymphoid tissues and detection of a moderate to high amount of PCV2 in damaged tissues. Since PMWS is considered a multifactorial disease in which other factors in addition to PCV2 are needed in most cases to trigger the clinical disease, effective control measures have focused on the understanding of the co-factors involved in individual farms and the control or elimination of these triggers. PDNS, an immuno-complex disease characterized by fibrino-necrotizing glomerulonephritis and systemic necrotizing vasculitis, has been linked to PCV2, but a definitive proof of this association is still lacking. PCV2-associated reproductive disease seems to occur very sporadically under field conditions, but it has been characterized by late-term abortions and stillbirths, extensive fibrosing and/or necrotizing myocarditis in fetuses and the presence of moderate to high amounts of PCV2 in these lesions. Taking into account that scientific information on PCV2 and its associated diseases has been markedly expanded in the last 8 years, the objective of this review is to summarize the current state of knowledge of the most relevant aspects of PCV2 biology and PCVD. 相似文献
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Sibila M Calsamiglia M Segalés J Blanchard P Badiella L Le Dimna M Jestin A Domingo M 《American journal of veterinary research》2004,65(1):88-92
OBJECTIVE: To determine whether correlations exist between viremia with porcine circovirus type 2 (PCV2) and serum antibody profiles and between detection of PCV2 in nasal cavities and viremia of pigs from farms with and without postweaning multisystemic wasting syndrome (PMWS). ANIMALS: 495 pigs, ranging from the late nursery stage to the early grower-finisher stage of production. PROCEDURE: Serum antibodies to PCV2 were studied with an ELISA that detects the ORF2 viral protein. Nasal swab specimens and serum samples were tested with a PCV2-specific PCR assay. RESULTS: PCV2 DNA and serum antibodies to PCV2 were detected in pigs from all farms, although in different proportions. Overall, PCV2 DNA was detected in greater percentages in serum samples and nasal swab specimens of pigs from farms with PMWS. Although viral DNA was detected in both serum samples and nasal swab specimens, PCV2 detection in nasal swab specimens was higher than in serum samples of pigs from all farms. Serum antibodies to PCV2 were detected in a greater percentage of pigs from farms with PMWS, compared with farms without PMWS. CONCLUSIONS AND CLINICAL RELEVANCE: A high prevalence of PCV2 infection was found in pigs from farms with and without PMWS. Besides the presence of PCV2, unknown additional factors may be necessary to induce the full expression of PMWS. 相似文献
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Sipos W Duvigneau JC Pietschmann P Schilcher F Hofbauer G Hartl RT Schmoll F 《Veterinary immunology and immunopathology》2005,107(3-4):303-313
Porcine dermatitis and nephropathy syndrome (PDNS) is broadly discussed as a porcine circovirus type 2 (PCV2)-associated disease, although PCV2, in contrast to postweaning multisystemic wasting syndrome (PMWS), has to date not been proven to be the aetiologic agent. In order to better understand the complex immunopathology of PDNS, the systemic cytokine expression profiles of (i) five pigs suffering from PDNS, (ii) five animals suffering from naturally acquired PMWS and (iii) five controls were investigated at mRNA and protein levels by means of multiplex real-time RT-PCR and flow cytometric intracellular cytokine detection, respectively. IL-1alpha, IL-6 and IFN-gamma mRNA expressions were found to be elevated in PDNS pigs. At the protein level, an increased capacity of peripheral blood mononuclear cells to produce IL-2, IL-4, IL-6, IL-12, TNF-alpha and IFN-gamma was evident. Hematological investigations revealed a hypochromic anemia while basophils and monocytes were relatively and neutrophils absolutely increased in PDNS pigs. PCV2 antibody levels did not differ significantly between PDNS and PMWS affected animals. Taken results together, the cytokine profile of the PDNS affected animals together with hematological data pointed towards a proinflammatory condition supporting a Th1 bias. Cytokine data of PMWS affected animals exhibited only minor non-significant differences when compared to controls, only IL-10 was significantly decreased at the mRNA level. 相似文献