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为了研究1日龄雏鸡同时免疫新流法三联疫苗与马立克氏病活疫苗对马立克氏病活疫苗免疫效果是否有干扰,试验将1日龄雏鸡分为5组,第1组商品蛋鸡不免疫,第2组SPF鸡不免疫,第3组商品蛋鸡仅免疫马立克氏病活疫苗,第4组商品蛋鸡同时接种新流法三联疫苗与马立克氏病活疫苗,第5组空白对照鸡不免疫,于雏鸡8日龄时对第1～4组用马立克氏病超强毒Md5株进行攻毒,攻毒后90 d内连续观察临床症状、剖检变化及病理组织学变化。结果表明:第1,2组鸡出现精神沉郁、肢体麻痹等临床症状并有部分鸡死亡,对死亡鸡只剖检可见,肝脏、脾脏肿大,全身器官出现肿瘤;对剖检鸡只进行组织病理学观察,可见典型的马立克肿瘤病变。而第3～5组鸡则无任何临床病症出现。说明无论是单独免疫马立克氏病活疫苗或是与新流法三联疫苗同时免疫,均能对鸡只提供良好保护,同时免疫新流法三联疫苗与马立克氏病活疫苗不会影响马立克氏病活疫苗的免疫效果。 相似文献
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某大型鸡场1993年用马立克氏病火鸡疱疹病毒疫苗（HVT）免疫鸡群后．其鸡马立克氏病（MI））发病率为7．2％。1994年改用美国苏威公司的免疫促进素ACM—1＋HVT冻干疫苗免疫鸣41．6万只，结果鸡群发病率为0.06％，很快控制了马立克氏病的流行。 相似文献
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每一位养鸡者，特别是种鸡饲养者最关心的大概就是怎样控制和预防鸡马立克氏病(MD)了。一般认为，控制和预防鸡马立克氏病有三大战略，即以疫苗免疫为中心，加强生物安全性，和选择有遗传抵抗力的鸡品种。那么，这样就能控制住鸡马立克氏病了吗?回答是不确定的。因为，自从1907年第一次发现鸡马立克氏病到现在近百年的时间里，我们一直不断地面临着新的挑战。那就是，随着疫苗的广泛应用和鸡品系的不断选育，鸡马立克氏病病毒由于自然选择和免疫压力，其毒力也在不断地增强，一次又一次地突破了疫苗所能提供的保护力。 相似文献
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鸡马立克氏病(MD)是鸡的一种传染性肿瘤疾病，本病的发生不均衡，一定地区可反复急性暴发。集约化养鸡(肉用仔鸡)地区该病的暴发率特别高，给养禽业造成很大经济损失。用马立克疫苗免疫鸡是预防鸡马立克氏病的主要方法。我国目前使用的疫苗主要是鸡马立克氏病火鸡疱疹病毒活疫苗。 相似文献
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１免疫失败原因鸡马立克氏病（ＭＤ） ,是危害养鸡业最严重的传染病之一 ,世界各养鸡国家和我国各省都有本病流行 ,２０世纪７０年代开始应用鸡马立克氏病疫苗以来 ,马立克氏病对养鸡业造成的损失已大大降低 ,但近年来 ,免疫失败时有发生 ,免疫失败的原因很复杂 ,但主要有以下几个方面 :１．１早期野毒感染雏鸡１日龄接种鸡马立克氏病疫苗２～３周后才能产生足够的免疫力 ,如果孵化室、鸡马立克氏病疫苗接种室、育雏舍环境卫生和消毒工作不好 ,被鸡马立克氏病毒污染 ,雏鸡早期感染鸡马立克氏病毒 ,免疫效果就不理想 ,甚至发生免疫失败。１．… 相似文献
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鸡马立克氏病是危害养禽业最为严重的免疫抑制与肿瘤病，也是能用疫苗免疫的肿瘤病。本文针对最近五年我国鸡马立克氏病的流行变异、免疫防控与未来发展进行总结分析，以期为国内家禽养殖业、生物制品行业提供参考。 相似文献
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鸡马立克氏病（MD）是鸡的一种高度接触性传染性并且以淋巴细胞增生为特征的病毒病。目前马立克氏病疫苗保护指数在64．44％－82．86％之间，因此，经马立克氏病疫苗免疫的鸡仍会有一小部分发生马立克氏病，但是，1997年初，福州，连江，三明，泉州，南平，厦门等12个县市的近130个养鸡场（户）所饲养的使用劣质马立克氏病疫苗免疫的约58．4万羽蛋鸡发生了较大规模的鸡马立克氏病（MD），使养鸡场户均蒙受了巨大经济损失，本次MD病流行出现了一定数量的鸡以腺胃肿大为特征，其它内脏肿瘤明显的单纯性腺胃肿瘤，特别是鸡龄越小的鸡越易出现此种病变。 相似文献
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动物红细胞免疫功能的研究 总被引：15，自引：0，他引：15
本文应用免疫花环试验测定了黑白花奶牛、水牛、鸡、鸭、小灵猫以及鸡传染性法氏囊病、马立克氏病和霍乱病红细胞上C_3b受体与免疫复合物的变化。检测了它们在红细胞上形成的花环。结果表明,动物因种属不同,红细胞上C_3b受体的数量有着极明显的差异,以鸭为最多,黑白花奶牛最少。动物患病后,红细胞上的C_3b受体可明显地减少。鸡传染性法氏囊病、霍乱病和马立克氏病皆非常明显地低于健康鸡。而红细胞上的免疫复合物,各种动物虽有差异,但差异不显著。患病鸡略低于健康鸡。 相似文献
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通过PCR方法扩增MDV Md11株的pp24基因,将其克隆到真核表达载体pcDNA3.1/zeo( )中.阳性克隆鉴定后,在脂质体作用下转染CEF细胞,用间接免疫荧光试验检测,结果证明阳性克隆在CEF细胞中表达了pp24蛋白. 相似文献
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O P Settnes 《Nordisk veterinaermedicin》1985,37(3):170-175
In chickens experimentally infected with Marek's Disease virus (MDV) an increased amount of immunoglobulin G is produced. Using a technique of quantitative crossed immunoelectrophoresis it has been shown, that 70% of this immunoglobulin G is non-specific. Only 18% could be absorbed with MDV strain CPRL VII-infected chicken kidney cells, and only 5% with MDV-induced lymphoblastoid cells of the MDCC-MSB1 cell line. It is hypothesized that the production the unspecific immunoglobulin G is caused by a polyclonal stimulation of B-cells. 相似文献
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Efficacy of a bivalent vaccine against Marek's disease 总被引：1，自引：0，他引：1
A bivalent vaccine was prepared by combining inactivated Marek's disease virus and turkey herpesvirus. The efficacy of this vaccine, compared to turkey herpesvirus and inactivated Marek's disease virus separately, was studied in unsexed White Leghorn chicks which were vaccinated at one day old and then challenged at 21 days old with fowl blood infected with virulent Marek's disease virus. The bivalent vaccine appreciably delayed mortality resulting from Marek's disease and elicited the highest protective efficacy as judged on the basis of Marek's disease-specific mortality and percentage occurrence of lesions. The occurrence, extent and severity of gross lymphomas and microscopic lymphoproliferative lesions in various organs of the bivalent vaccinated birds were less than in the other challenged groups. In addition, the level of viraemia remained consistently and significantly lower in the bivalent vaccinated birds. 相似文献
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An experiment was performed to study the immunogenicity of the dermal antigen of Marek's disease virus, extracted from the skin of 30-day-old chickens, infected with Marek's disease virus on the first day of life. Three kinds of samples were tested: (1) dermal antigen centrifuged at 10 000 g per 0.5 h, (2) dermal antigen centrifugated at 10 000 g per 0.5 h and 100 000 g per 1 h, (3) dermal antigen treated like sample (2) and partly purified by DEAE-cellulose chromatography. Samples (1) and (2) were inoculated to two-day-old chickens and the vaccination was repeated, using complete Freund's adjuvant, 21 days later. Sample (3) was inoculated to two-day-old chickens with DEAE-dextran. All the three groups were challenged together with the controls (non-vaccinated chickens) on the seventh day after the first vaccination. A reduction of mortality was observed in the chickens vaccinated with and re-vaccinated with sample (1) (23.07%) and in the chickens vaccinated with sample (3) (30.76%). The chickens of the latter group were the last to start dying from Marek's disease--only after the 10th week of life. In the chickens which had been vaccinated and revaccinated with sample (2) the mortality was not reduced. The study is continued, with particular emphasis on the relationship of DEAE-dextran to protection against Marek's disease. 相似文献
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In a field survey of viraemias due to vaccination of chickens with herpes virus of turkey, variation was encountered in titres and percentages of birds viraemic. The incidence of viraemias was much lower in sick than in healthy birds in flocks undergoing mortality from Marek's disease. In a concurrent experiment the same strain of chicken and the same commercial vaccine were used as in the field flocks affected with Marek's disease. A high incidence of viraemia and 84.6 per cent protection against Marek's disease were obtained with single vaccination at one day of age. Revaccination at 21 days of age produced no measurable benefits in the same experiment. 相似文献
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根据马立克氏病病毒（MDV）国际参考强毒CA株pp24基因序列，设计和合成了一对引物，其两端分别含SalI和EcoRI的酶切位点，以CV1988株基因组DNA为模板，通过PCR技术，扩增其pp24基因。PCR产物经纯化后，按正确的阅读框架定向克隆到表达性载体pGEX-6P-1中谷胱甘肽转移酶（GST）基因的下游，并用序列测定验证。将重组质粒转化的大肠杆菌BL21，在1.0mMIPTG和37℃条件下诱导，GST-pp24基因获得了表达。诱导菌体的裂解物经12%的SDS聚丙烯凝胶电泳（SDS-PAGE）和Westemblot试验验证。得到大小为43kD的融合蛋白，与预期大小一致。将该融合蛋白的凝胶带切下研碎后免疫小鼠三次后，其血清与MDV感染的鸡胚成纤维细胞（CEF）在间接免疫荧光试验中呈阳性反应。 相似文献
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鸡肿瘤病料中马立克氏病病毒和禽网状内皮组织增生症病毒共感染的研究 总被引：24，自引：1，他引：23
采用细胞培养、间接荧光抗体试验(IFA)、聚合酶链式反应(PCR)和斑点杂交(Dotblot)的方法从我国不同地区发生肿瘤的病料中同时进行MDV和REV的分离和鉴定。在分离到的13株MDV野毒株中，有4株培养物既能在IFA中与REV的单抗反应，又可以用PCR扩增出REV的LTR；另有4株培养物能扩增出REV的LTR，但在IFA中却不与REV的单抗反应。结果表明我国MD肿瘤中存在着REV的共感染，且我国MDV某些野毒株的基因组中有可能已经整合进了REV的LTR序列。 相似文献