共查询到19条相似文献，搜索用时 125 毫秒
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白僵蚕体内所含的草酸铵为抗惊厥的有效药用成分。采用响应面法优化了白僵蚕草酸铵的提取工艺条件。在单因素试验的基础上,选择提取温度、提取时间、原料质量浓度3个因素进行中心组合试验,建立了草酸铵得率的二次回归方程。此模型拟合性好,通过响应面分析以及岭嵴分析得到了优化组合条件。试验结果表明,提取温度及原料质量浓度对草酸铵得率都有显著影响,当提取工艺条件为温度85℃,提取时间65 min,原料质量浓度43 g/L时,草酸铵得率达到最大值。此条件下草酸铵得率验证值为3.25%,与预测值3.29%基本一致。 相似文献
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为探究不同炮制白僵蚕中草酸铵和白僵菌素含量与外观性状的相关性,采用HPLC-UV法分别测定不同批次白僵蚕与炒僵蚕中草酸铵和白僵菌素的含量,利用SPSS 26.0软件将白僵蚕和炒僵蚕的单体质量、长度、直径、丝腺环数与草酸铵和白僵菌素含量进行相关性分析。结果表明,白僵蚕中草酸铵和白僵菌素含量呈显著正相关,且这两个成分含量与白僵蚕外观性状也均呈显著正相关;炒僵蚕中白僵菌素含量与外观性状呈显著正相关,而草酸铵含量与白僵菌素含量及各外观性状指标均无相关关系。该结果可为白僵蚕、炒僵蚕饮片质量评价提供参考,也可为炒僵蚕炮制工艺的规范提供线索。 相似文献
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一株家蚕病原白僵菌的鉴定及人工生产白僵蚕初探 总被引：1，自引：0，他引：1
《蚕业科学》2020,(2)
白僵蚕作为传统中药材被用于治疗多种疾病,因此筛选应用于白僵蚕人工培养的优良白僵菌具有重要的实践意义。从僵蚕分离获得一株白僵菌,经形态学观察和菌株rDNA ITS的系统发育分析,鉴定为球孢白僵菌(Beauveria bassiana),命名为Beauveria bassiana GX-1。进一步研究菌株的营养生长量、产孢时间、产孢量等与致病力相关的部分生物学特性。结合不同浓度的白僵菌孢子悬浮液对家蚕5龄起蚕幼虫接种的致死率和2次中试试验的结果,确认人工生产白僵蚕孢子的接种量应为1×10~7个/mL,致死率达到95%以上。2次中试产品经HPLC法测定其草酸铵质量分数分别为6.26%和6.00%,物理和化学指标均达到《中华人民共和国药典》标准。研究结果为人工培养白僵蚕,提高白僵蚕的产量和品质奠定了实验基础。 相似文献
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中国野桑蚕滞育激素基因的克隆与序列分析 总被引：1，自引：0，他引：1
根据家蚕DH PBAN基因序列设计特异引物 ,以中国野桑蚕基因组DNA为模板进行PCR扩增 ,获得了1 5 85kb的目的片段 ,将其克隆进pMD18 T载体 ,测序和同源性比较结果表明 :该片段由 3个外显子、2个内含子组成 ,2个内含子均为典型的GT AG结构 :该片段编码中国野桑蚕滞育激素 (DH)及DH引导肽 ;中国野桑蚕DH及引导肽与家蚕及日本野桑蚕的DH及引导肽氨基酸序列完全相同 ,其基因cDNA序列的同源性分别为 99 3%、97 2 %。研究结果进一步显示了中国野桑蚕与家蚕之间的近缘关系。 相似文献
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基于mtDNA ND1基因的家蚕进化分析 总被引：1，自引：0，他引：1
对中国青州野桑蚕mtDNA中ND1基因进行了克隆和序列分析。结果显示,野桑蚕ND1基因全长为933bp,编码310个氨基酸残基,A+T含量较高,达78.7%;同源性分析显示,不同来源的家蚕和野蚕ND1基因在核苷酸水平和氨基酸水平具有很高的相似性;以ND1基因的核苷酸序列及氨基酸残基序列进行了家蚕及野桑蚕的分子进化分析,进一步证明家蚕起源于中国野桑蚕。用mtDNA的ND1基因的核苷酸序列Blast家蚕的基因组序列,发现家蚕基因组中存在与ND1基因部分区域同源的序列,表明家蚕基因组中存在起源于线粒体的假基因。 相似文献
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老挝家蚕线粒体cox3基因序列分析及分子进化研究初探 总被引：2，自引：0，他引：2
对老挝家蚕品种L11的线粒体DNA(mitochondrial DNA,mtDNA)cox3基因(cox3)进行PCR扩增、克隆和序列分析。结果表明,在测定的909bp的片段中,含有ATPase 6基因及tRNA-Gly基因的部分序列和cox3全序列。其中,cox3读码框包括789个核苷酸,编码262个氨基酸。L11 cox3和已经公布的家蚕(中国家蚕品种夏芳、日本家蚕Aojuku、韩国家蚕Backojam)mtDNAcox3基因完全一致,但与日本野桑蚕、中国野桑蚕的氨基酸同源性分别为99%和98%。 相似文献
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中国野桑蚕和日本野桑蚕的RAPD研究 总被引：12，自引：5，他引：7
用RAPD PCR技术初步研究了中国野桑蚕与日本福冈野桑蚕之间的DNA多态性。结果表明 ,不同地域中国野桑蚕个体之间及其与日本福冈野桑蚕个体之间均呈现出丰富的DNA多态性。中国野桑蚕同家蚕共有带率达 3 5 5 % ,同日本福冈野桑蚕为 4 5 1% ,而日本福冈野桑蚕同家蚕为 3 2 % ;中国野桑蚕与家蚕的平均遗传距离为0 5 2 ,日本野桑蚕与家蚕的平均遗传距离为 0 6 3,与中国野桑蚕的为 0 5 8,而且与中国沈阳地区野桑蚕之间的遗传距离最近 ,为 0 4 8,以此创建了它们的系统进化树。 相似文献
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Ralgph C. Weichselbaum DVM Daniel A. Feeney DVM MS CarlR. Jessen CarlA. Osborne DVM PhD ElizabethD. Dunphy DVM JosephW. Bartges DVM PhD 《Veterinary radiology & ultrasound》1998,39(5):396-411
Nine pure mineral types of canine uroliths (bladder or urethral origin only) identified in a chronologic sample from the Minnesota Urolith Center were compared to sequential dilutions of iodinated radiographic contrast medium in vitro. The uroliths studied were those composed of 100% magnesium ammonium phosphate, calcium oxalate monohydrate, calcium oxalate dihydrate, calcium phosphate appatite, calcium hydrogen phosphate dihydrate (brushite), ammonium acid urate, sodium acid urate, cystine, and silica. The radiopacity of the uroliths was classified as radiolucent, isopaque, or radiopaque, as compared to the radiopacity of the contrast medium solutions in which they were placed, using 2.0 mm and 5.0 mm depths in petri dishes radiographed using a table-top technique. A statistically significant relationship was found between the effective atomic number of the uroliths and the effective atomic number of the contrast medium solutions to which they were compared for the endpoints of isopacity, first luceney (in increasing iodine concentration sequence), and optimal visualization of internal architecture. In general, uroliths isopaque or radiolucent in contrast medium solutions weaker than 23.5 mgI2/ml are most likely ammonium acid urate or sodium acid urate. Uroliths isopaque or radiolucent in contrast medium solutions between 23.5 mgI2/ml and 44.4 mgI2/ml are probably magnesium ammonium phosphate, cystine, or silica. Uroliths that remained radiopaque in solutions stronger than 44.4 mgI2/ml, and particularly those radiopaque in contrast medium solutions stronger than 80 mgI2/ml, almost always contained calcium. This relative opacity assessment is proposed for use in double contrast cystography as an aid in differentiating urolith mineral types clinically to facilitate appropriate use of medical protocols to dissolve uroliths or to prevent their growth or recurrence. 相似文献
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根据氟化钠抑制血液葡萄糖分解,但使红细胞体积缩小,而草酸铵使红细胞体积膨胀的原理,设计和研究出一种氟化钠-草酸铵抗凝剂新配方(1:5.2)。经乳牛、牦牛、绵羊、猪、马和兔血液的试验表明,这种抗凝剂新配方对 PCV 无影响,又能有效地抑制血液葡萄糖分解。 相似文献
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J P Lulich C A Osborne D J Polzin S D Johnston M L Parker 《American journal of veterinary research》1991,52(10):1573-1578
Twenty-four-hour excretion of urine metabolites was determined in 33 clinically normal Beagles during periods of consumption of a standard diet and when food was withheld. The goal was to determine normal canine values for urine analytes incriminated in the genesis of calcium oxalate uroliths. During periods when dogs consumed food, dairy urinary excretion of calcium, uric acid, sodium, potassium, magnesium, ammonium, and hydrogen ions were significantly (P = 0.0004, 0.0038, 0.001, 0.0001, 0.0004, 0.0001, and 0.024, respectively) higher than when food was withheld. Urinary excretion of phosphorus, oxalate, and citrate were not significantly different between samples obtained during periods of food consumption and when food was withheld. Male dogs excreted significantly higher quantities of urine oxalate than females during fed (P = 0.003) and nonfed (P = 0.003) conditions. When food was withheld, urinary uric acid excretion was significantly higher in males than females (P = 0.01). Females excreted significantly more urine calcium than males when food was withheld (P = 0.003). Our results indicated that dietary conditions influence the quantity of sodium, potassium, calcium, magnesium, and uric acid excreted in the urine of clinically normal dogs; therefore, dietary conditions should be considered when measuring the concentration of these analytes in urine. 相似文献
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HPLC法测定中兽药散剂中非法添加土霉素的方法研究 总被引：2，自引：1，他引：1
建立了高效液相色谱法检测清肺散、白头翁散、止痢散等中兽药散剂中非法添加土霉素的方法。用十八烷基硅烷键合硅胶色谱柱,以0.05 mol/L草酸铵溶液-二甲基甲酰胺-0.2 mol/L磷酸氢二铵（75∶20∶5）（用氨试液调pH值至8.0±0.2）为流动相,检测波长280 nm,流速1.0mL/min,进样量20μL,柱温35℃。结果显示,土霉素的线性范围为100～300μg/mL,线性方程为Y=4.90×10 X-2.39×103（,r=0.999 6,n=5）,本方法的回收率为80.68%～119.23%,RSD为0.66%～1.85%。结果表明,本方法准确、可靠,适用于中兽药散剂中非法添加土霉素的检测。 相似文献