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为建立一种合适的猪附红细胞体病动物模型，利用摘除脾和／或注射地塞米松的昆明小白鼠，以腹腔注射方式人工感染猪附红细胞体，通过血液涂片镜检、PCR检测、临床症状观察及病理剖检对感染情况进行了鉴定。结果显示，猪附红细胞体能够经腹腔注射感染昆明小白鼠，且感染鼠表现出与猪附红细胞体病相似的临床症状。结果表明，猪附红细胞体实验动物模型已成功建立，并证实啮齿类动物在附红细胞体的传播过程中发挥着重耍作用。 相似文献
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为探究猪附红细胞体感染特性,进而优化猪附红细胞体小鼠感染模型建立的条件,本试验设计了不同小鼠处理方式感染试验（A试验）、不同病原形式感染试验（B试验）、冻存病原感染试验（C试验）和小鼠模型血液再感染小鼠试验（D试验）。通过对每个试验中各组小鼠血液感染情况、感染首现时间、临床症状和平均感染时间的综合评估,分析不同小鼠处理方式、不同病原形式、冻存病原及小鼠模型血液再感染是否对小鼠模型的建立有影响。通过PCR检测、电镜观察和特异基因片段序列测定,进一步鉴定小鼠模型感染的病原与猪附红细胞体是否一致。结果显示,A试验中,昆明小鼠切除脾脏组感染效果最优;B试验中,猪附红细胞体阳性血液样本组感染效果最优;C试验中,阳性血液未冻存组感染效果优于冻存组;D试验中,猪阳性血液样本组、小鼠模型阳性血液样本组无明显差异。PCR检测、电镜观察和特异基因片段序列测定结果显示,小鼠模型感染的病原与猪附红细胞体一致。结果表明,不同小鼠处理方式、不同病原形式和病原的冻存等条件对猪附红细胞体小鼠感染模型的感染效果均有影响,通过对比,以猪阳性血液感染切除脾脏的昆明小鼠的方式建立小鼠感染模型效果最优。 相似文献
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绵羊附红细胞体病是由绵羊附红细胞体寄生于红细胞表面、血浆、骨髓中引起的一种血液传染病。绵羊感染附红细胞体后,一般不出现明显的临床症状,仅表现食欲下降,持续性腹泻,可视粘膜及口唇粘膜苍白和黄染,羔羊机体消瘦,生长发育缓慢;急性病例临床症状典型,严重病例出现溶血性贫血,死亡,给羊场或牧民群众造成较大的经济损失。 相似文献
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人工感染猪附红细胞体病的病理组织学研究 总被引：1，自引：1，他引：0
为观察昆明小鼠人工感染猪附红细胞体后的病理组织学变化。用常规病理学方法,对6只腹腔注射感染猪附红细胞体的小鼠进行病理解剖学和病理组织学研究,光镜下观察并描述小鼠红细胞感染附红细胞体后的形态变化。剖检可见主要器官和皮下瘀血,胸腹部皮下脂肪黄染,病理组织学变化的主要特征是心、肝、脾、肺、肾等主要脏器实质细胞变性、坏死,毛细血管扩张充血、出血,脾窦内含铁血黄素沉着。结果表明,猪附红细胞体感染小鼠模型的成功建立,镜下红细胞的形态特征及主要器官剖检和病理组织学变化与文献报道的猪附红细胞致病特征基本吻合,存在的差异可能与不同地域猪附红细胞体病原株致病性有关。 相似文献
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猪附红细胞体对不同宿主红细胞的体外感染试验 总被引：1，自引：0，他引：1
为证实猪附红细胞体能否感染其它宿主红细胞,本试验在猪附红细胞体体外培养的基础上,进行了猪附红细胞体体外感染家兔、昆明小白鼠、犬、羊、牛及人的健康红细胞。结果表明,将感染猪附红细胞体的阳性血液体外感染家兔、昆明小白鼠、犬、羊、牛及人的健康红细胞,均可不同程度的感染,其中以兔和昆明小白鼠红细胞的感染率最高,分别达45.0%和40.3%;人红细胞的感染率为30.0%,呈现轻度感染;而对其它宿主红细胞,呈现一过性感染。 相似文献
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猪犬人附红细胞体对小白鼠的感染性试验 总被引：5，自引：0，他引：5
选健康小白鼠30只，随机分为9组。Al、A2、A3组分别荐臀部皮下注射患附红细胞体病的猪、犬、人的全血。并用感染附红细胞体小白鼠的全血分别接种B1、B2、B3组，其余3组分别混笼、饲料中添加患附红细胞体病猪的全血、在被感染附红细胞体小白鼠污染的垫料中饲养。结果表明，猪、犬、人附红细胞体均可以感染小白鼠，被感染的小白鼠也可以再感染同种动物；经母鼠垂直感染胎儿；荐臀部皮下注射和食入病原是直接的感染途径。直接接触也可以传播本病。 相似文献
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正羊附红细胞体病是附红细胞体感染羊所引起的一种传染性人畜共患病,附红细胞体寄生于羊的红细胞表面或血浆,主要表现为溶血性贫血、黄疸、生长速度降低和精神抑郁等症状。该病病原主要由吸血的节肢动物传播,可感染绵羊和山羊。但是,本次发病观察发现,绵羊对此病抵抗力较强,山羊容易感染。 相似文献
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本试验为建立一种高效的羊附红细胞体解离方法,采集红细胞感染率大于90%的阳性抗凝血,分别应用水浴法和药物体外驱虫法对羊附红细胞体进行解离,观察分离前后附红细胞体感染率、红细胞感染强度、附红细胞体数、杂质含量和附红细胞体的运动性5项指标;提取附红细胞体抗原,制备全蛋白悬液,测定蛋白质含量,比较解离效果。将两种方法制备的羊附红细胞体抗原全蛋白进行SDS-PAGE试验,观察条带是否一致。结果显示,200 mL血液中加入1 mL双向红莲灭,4℃作用36 h,即可达到较好分离效果。与水浴法相比,解离后,红细胞感染率和感染强度明显下降,蛋白含量增加,收率高,纯度好。SDS-PAGE结果显示,体外驱虫法所制的羊附红细胞体抗原全蛋白悬液,其抗原蛋白带与水浴法相同,因此可用于粗制羊附红细胞体抗原。 相似文献
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猪附红细胞体病是由猪附红细胞体感染机体引起的一种人畜共患的传染病。而附红细胞体则是寄生于红细胞表面、血浆、及骨髓中的一类微生物。近年来随着我国养猪业的蓬勃发展,该病的流行已有越来越烈之势,并逐渐成为危害养猪业的传染病之一。为保证群众养猪业的快速和健康发展,必须引起充分重视,予以科学诊治。 相似文献
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THE EFFECT OF EPERYTHROZOON OVIS INFECTION ON THE GLUCOSE LEVEL AND SOME ACID-BASE FACTORS IN THE VENOUS BLOOD OF SHEEP 总被引：1，自引：0，他引：1
R. H. Sutton 《Australian veterinary journal》1977,53(10):478-481
Eperythrozoon ovis infected sheep have low venous blood glucose levels and correspondingly increased blood lactic acid levels as compared with control sheep. Acid-base studies showed that these changes were accompanied by significant falls in venous pH, and standard bicarbonate as well as a negative base excess. All these changes were considered to result from the increased alvcolytic activity of infected erythrocytes. The acidosis and hypoglycaemia associated with E. ovis infection, while not having any apparent effect on young, well-fed sheep, could be potentially serious in pregnant ewes and in sheep on a low plane of nutrition. 相似文献
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A modified indirect immunofluorescent assay for the detection of antibody to Eperythrozoon ovis in sheep 总被引：2，自引：0，他引：2
Experimental ovine eperythrozoonosis was studied using Giemsa staining of blood films and a modified indirect immunofluorescent antibody assay (IFAA). The serums of 21 Border Leicester Merino cross lambs between 12 weeks and 7 months-of-age were analysed before and after infection with Eperythrozoon ovis (E. ovis) using the IFAA test. No rise in the IFAA titre was seen until day 7 and this coincided with the first detection of E. ovis organisms in blood smears stained with Giemsa. The percentage of E. ovis infected red blood cells peaked on day 14, but the IFAA titre did not peak until day 35. Titres to E. ovis, on average, had begun to drop by day 63. There was considerable individual variation in response to E. ovis infection as measured by the IFAA. Titres as high as 6,400 were observed in individual sheep at the peak of E. ovis parasitaemia of red cells. One sheep had a titre of 51,200 nineteen days after infection, and titres of 3,000 were maintained for several months in a few sheep. The assay proved reliable, and up to 100 samples per day could be tested. The antigenicity of the slide preparations was found to be satisfactory after storage for 6 months at -20 degrees C and 4 degrees C and for 28 months at -70 degrees C. Temperature fluctuations during storage rendered slides unsuitable for the IFAA after these times. A method of storing E. ovis infected blood in liquid nitrogen is described. 相似文献
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为快速准确诊断和检测羊附红细胞体病,及时采取防治措施,建立了检测羊附红细胞体抗原的双抗夹心ELISA诊断方法。选取规模化养殖场羊,镜检附红细胞体红细胞感染率> 90%,无菌采取血液,分离羊附红细胞体抗原,制备纯化兔抗羊附红细胞体抗体,应用辣根过氧化物酶标记抗体,进行双抗体夹心ELISA试验。试验结果表明,双抗体夹心ELISA方法的最佳工作条件为:抗体最佳包被量为82.91 μg/mL,酶标抗体最适工作浓度为1∶400,抗原最低检出量为7.81 μg/mL;而且与支原体、大肠杆菌、葡萄球菌以及牛、猪、兔附红细胞体均不出现交叉反应,表明该方法具有良好的特异性,可用于羊附红细胞体病的诊断和群体检测。 相似文献
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Changing morphology of Eperythrozoon ovis 总被引：5，自引：0，他引：5
Light microscopy studies of Eperythrozoon ovis in sheep revealed that Giemsa stain was only less reliable than acridine orange as a means of parasite identification when low parasitaemias were present. The morphology of E ovis altered as the degree of parasitaemia increased. 相似文献
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Effect of Eperythrozoon ovis on the lysis of sheep erythrocytes in the complement fixation test 总被引：2，自引：0，他引：2
M J Norris R S Rahaley R G Whittaker 《Veterinary immunology and immunopathology》1987,16(3-4):283-288
When erythrocytes from sheep experimentally infected with Eperythrozoon ovis were used in the titration of reagents for a standardised complement fixation test, increased amounts of both haemolysin and complement were required for erythrocyte lysis compared with preinfection titrations. The haemolysin requirement increased by up to 125% at 55 days post-infection and complement requirement increased by up to 40% at 40 days post-infection. These changes appeared to correlate with the development of a macrocytic anaemia in affected sheep rather than E. ovis parasitaemia. The results emphasise the need to carefully monitor the haematological parameters of sheep used as sources of erythrocytes for the complement fixation test. 相似文献
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A total of 126 lamb carcases, of which 80 were jaundiced and 46 were grossly normal at routine meat inspection, were examined. Two specific diseases were demonstrated to be associated with jaundiced carcases. Eperythrozoon infection was demonstrated in 65% of jaundiced, and 12% of non-jaundiced carcases from jaundice-affected lots, but not in 5 normal carcases from unaffected killing lots. Copper poisoning was demonstrated in 2 of the jaundiced carcases. Infection with Eperythrozoon ovis was therefore the condition most commonly associated with, and presumably the major cause of jaundice in these lamb carcases. Copper poisoning was a less common cause of jaundice. 相似文献
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Circulating antibody was detected by an indirect fluorescent antibody test (IFAT) in the serum of sheep infected experimentally with Eperythrozoon ovis. Antibodies were first detected 15 to 32 days after infection with E ovis and titres peaked at 41 days. This antibody may be associated, at least in part, with protection against infection with E ovis since the initial increase in antibody titre coincided with a fall in the primary parasitaemia. A role for antibody is suggested further by the fact that the prepatent period of infection was prolonged by one day and the parasitaemia initially remained at low levels in infected sheep protected by passively transferred hyperimmune serum. Moreover, following primary infection, acquired immunity was manifest by a lack of parasitaemia following challenge infections while increased IFA titres were observed. No evidence of opsonic activity was observed in an in vitro erythrophagocytosis test in that neither mouse macrophages nor sheep monocytes phagocytosed E ovis infected or uninfected erythrocytes sensitised with hyperimmune serum. 相似文献