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1.
为摸清张家口坝上地区鸡、兔弓形虫病的感染情况,采用间接血凝试验(IHA)对张家口坝上地区散养鸡、笼养鸡、家兔、兔场兔的745份血清进行弓形虫血清学检测。结果表明,散养鸡阳性率6.6%(24/364);笼养鸡阳性率0%(0/120);家兔阳性率12.5%(22/175);兔场兔阳性率2.3%(2/86)。其中阳性散养鸡血清抗体滴度分布在1:256有25%(6/24),阳性家兔血清抗体滴度分布在1:256有9.1%(2/22)。 相似文献
2.
应用乳胶凝集试验(LAT)对湖北省荆州市城区或城郊农产品集贸市场中鸡的弓形虫抗体进行血清学调查,共检测鸡血清286羽份。结果表明,阳性血清72羽份,平均阳性率为25.17%,其中,散养鸡的阳性率为38.95%,极显著高于笼养鸡的18.32%(P0.01),产蛋鸡的阳性率为30.13%,极显著高于肉用鸡的19.23%(P0.01)。 相似文献
3.
本研究是在Dubey J P所建改良凝集试验基础上,采用小鼠肉瘤S180细胞培养弓形虫速殖子,甲醛固定抗原后悬浮于碱性缓冲液,用2-巯基乙醇处理待测血清,用该法对610份鸡血清进行弓形虫抗体检测,并用间接血凝试验(IHA)作对照。结果显示,MAT、IHA对鸡血清的弓形虫抗体检测阳性率分别为9.02%(55/610)、1.64%(10/610),二者差异极显著(P<0.01)。MAT对鸡的弓形虫抗体检测结果,鸡血清阳性率为9.02%(55/610),其中散养鸡阳性率为18.75%(30/160),笼养鸡阳性率为5.56%(25/450),二者差异极显著(P<0.01);蛋鸡和种鸡阳性率分别为7.89%(15/190)和8.00%(8/100)、肉鸡阳性率1.25%(2/160),与肉鸡相比差异显著(P<0.05)。 相似文献
4.
正为了较全面了解信阳地区鸡弓形虫病的流行情况,对信阳市的鸡弓形虫病提出合理的防控措施,为期一年的时间应用间接血凝试验(IHA方法),对不同规模鸡场以及散养户3774份血清样本进行了检测调查。结果显示:受检的3774份鸡血清样本中阳性样本536份,阳性率为14.2%;在所调查的9个县区中,鸡弓形虫感染率最高的是罗山县,阳性率可达21%;新县最低,阳性率8.1%。 相似文献
5.
为了解荆州市鸡弓形虫病的流行情况，应用乳胶凝集试验（LAT）对荆州市城区和城郊农产品集贸市场上鸡的弓形虫抗体进行血清学调查，共检测鸡血清286羽份。结果表明，阳性血清72羽份，平均阳性率为25．17％，其中，散养鸡的阳性率为38．95％，极显著高于笼养鸡的18．32％（P〈0．01），产蛋鸡的阳性率为30．13％，极显著高于肉用鸡的19．23％（P〈0．01）。 相似文献
6.
通过调查河南省散养鸡新孢子虫病和弓形虫病的血清与分子流行病学情况,评估该地区土壤受新孢子虫和弓形虫污染的程度。分别采用IFAT和MAT方法检测散养鸡血清中的新孢子虫和弓形虫的抗体,PCR方法检测部分血清阳性样品消化液的弓形虫抗原。结果发现,新孢子虫病的阳性率为23.26%(150/642;滴度1∶25;CI 95%=20.25~26.79),弓形虫的感染率为18.86%(132/700;滴度1∶25;CI 95%=16.13~21.93)。PCR检测弓形虫的结果为16%(4/25)。弓形虫感染与散养鸡的性别有关,公鸡感染率显著高于母鸡感染率(P<0.05)。结果表明,散养鸡的新孢子虫病和弓形虫病的感染率均较高,该地区人畜面临着较高的新孢子虫和弓形虫卵囊污染的风险,公共卫生安全形势严峻。散养鸡的新孢子虫病流行情况在国内首次报道。 相似文献
7.
为了了解散养鸡弓形虫的感染情况以及鸡和麻雀感染弓形虫的相关性,在某散养鸡场内随机对100只鸡和在鸡场内捕获的100只麻雀,采用间接血凝试验进行了弓形虫感染的血清学检测。结果表明:散养鸡弓形虫抗体阳性率为12%,麻雀弓形虫抗体阳性率为3%。同一场地的鸡和麻雀弓形虫感染可能具有一定的相关性。 相似文献
8.
本试验旨在研究散养与笼养对产蛋鸡内脏器官发育、繁殖性状、血清生化指标的影响。选取25周龄笼养蛋鸡180只,随机分为2组,每组6个重复,每个重复15只,将其中一组放至地面进行散养。试验第42天,每个重复中随机选取4只鸡,翅静脉采血,然后屠宰并测定内脏器官发育及繁殖性状的相关指标。结果表明:散养鸡体重极显著小于笼养鸡(P<0.01),肌胃指数、肝脏指数、十二指肠指数、空肠指数、回肠指数、盲肠指数极显著大于笼养鸡(P<0.01),腺胃指数、直肠指数显著大于笼养鸡(P<0.05);散养鸡产蛋率、输卵管重量、卵泡重极显著小于笼养鸡(P<0.01),输卵管长度显著小于笼养鸡(P<0.05);散养鸡血清甘油三酯含量显著低于笼养鸡(P<0.05),但白细胞介素-2含量与笼养鸡相比有提高的趋势(P>0.05)。结果提示,与笼养相比,散养降低了鸡体重,增大了内脏器官指数,降低了繁殖性能,对机体的健康状况有一定改善作用。 相似文献
9.
应用间接血凝试验(IHA)对松江区13个镇(街道)的禽类养殖户及3个规模养鸡场的580份禽类血清进行了弓形虫感染抗体检测,共检出抗体阳性血清91份,总抗体阳性率为15.69%(其中鸡抗体阳性率为10.03%、鸭抗体阳性率为26.54%、鸽抗体阳性率为5.0%)。结果表明,松江区禽类养殖场(户)均存在不同程度的弓形虫感染,且散养鸡的阳性率明显高于规模场鸡,散养鸭的阳性率明显高于散养鸡。 相似文献
10.
为摸清福建省家兔主要养殖地区弓形虫病的感染情况,采用间接血凝试验(IHA)对福建4个地区大型兔场、散养农户兔场的690份血清进行弓形虫病血清学检测.被检血清抗体滴度大于或等于1∶64判为阳性.结果表明:福建省4个主要养殖地区兔场弓形虫病阳性率9.71％(67/690)；其中,大型兔场阳性率1.73％(8/462),散养农户兔场阳性率25.88％(59/228). 相似文献
11.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
12.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
17.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
20.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
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