共查询到20条相似文献，搜索用时 31 毫秒
1.
Five strains of Theileria annulata from three geographically different areas and one strain of T parva were grown in bovine lymphoblastoid cell lines. Lysates of the parasitised cells were examined by thin layer starch gel electrophoresis for multiple forms of the enzyme glucose phosphate isomerase. A reported difference between the glucose phosphate isomerase isoenzyme patterns of T annulata and T parva was confirmed. Cultures of one strain of T annulata grown in cells derived from four different cattle showed similar host and parasite isoenzyme patterns. Two strains of T annulata and one strain of T parva grown in lymphoid cells derived from the same animal showed identical host cell isoenzyme patterns whereas the isoenzyme pattern associated with each parasite was different. Strains of T annulata from different geographical areas showed major differences in their isoenzyme patterns, but no differences were detected between strains from the same geographical area. Meldola blue was found to be superior to phenazine methosulphate as an intermediate electron acceptor in the visualisation of enzyme activity. 相似文献
2.
W G Jura 《Veterinary parasitology》1986,22(3-4):203-214
Interactions between Theileria annulata sporozoites and lymphoblastoid cell lines already transformed by the Hissar and Ankara strains of T. annulata [T. a. (H) and T.A. (A), respectively] and the Muguga strain of T. parva [T.P. (M)] were studied in vitro. Although sporozoites of the Hissar strain of T. annulata attached to and entered peripheral blood lymphocytes (PBL) and lymphoblastoid cell lines transformed by T. a. (H) and T. a. (A), they neither attached to nor entered the T. p. (M) cell line. Whether the superinfecting T. a. (H) sporozoites developed intracellularly was studied by monitoring daily changes of mean schizont nuclear numbers and by determining electrophoretic mobilities of schizont glucose phosphate isomerase in each cell line using thin-layer starch gel electrophoresis. While the mean schizont nuclear number in freshly-infected PBL underwent a steady increase to the level of those in long standing T. annulata cultures, analysis of variance of similar data in T. a. (H) and T. a. (A) cell lines in which superinfection was demonstrated revealed no significant differences between them and their respective control counterparts, i.e., T. a. (H) and T. a. (A) cultures with no superinfection. Enzyme polymorphism studies showed the formation of uncontaminated species- or strain-specific bands of glucose phosphate isomerase (GPI) isoenzyme activity in the T. p. (M) and in the superinfected T. annulata cell lines. 相似文献
3.
A colorimetric assay was developed for quantitating bovine neutrophil bactericidal activity against Staphylococcus aureus. The procedure used the tetrazolium compound, 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). The assay was conducted by incubating antibody-opsonized S. aureus with neutrophils in microtiter plates for 1 h at a ratio of 10 bacteria per neutrophil. Neutrophils were then lysed with saponin. The MTT was added and samples were incubated for 10 min. Live S. aureus reduced MTT to purple formazan. Dead bacteria and lysed neutrophils did not react with MTT. Bacterially-reduced formazan was solubilized by adding isopropanol and formazan production was quantitated by measuring absorption at 560 nm. Absorption of formazan was directly related to viable bacteria cell number and was used to determine the number of S. aureus not killed by neutrophils. The percentage of bacteria killed by neutrophils was determined by extrapolation from a standard formazan curve that was derived by incubating MTT with known numbers of S. aureus. The colorimetric MTT assay detected suppressed bactericidal activity after in vitro treatment of bovine neutrophils with colchicine, cytochalasin B, or phorbol 12-myristate 13-acetate. In vitro treatment of neutrophils with low levels of recombinant bovine interferon gamma (rBoIFN-gamma) enhanced bactericidal activity, whereas high levels decreased activity. These results suggest the colorimetric MTT bactericidal assay is efficacious in detecting modulation of bovine neutrophil bactericidal activity. Furthermore, the MTT assay has many advantages over traditional bactericidal assays in that it is sensitive, inexpensive, requires less than 3 h to complete, and can analyze many neutrophil samples in a single day. 相似文献
4.
The aim of the present study was to examine whether the 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyl tetrazolium bromide (MTT) colorimetric assay can be applied to measurement of mitogen‐induced chicken splenocyte activation. Activation was also measured by a 3H‐thymidine uptake assay and a viable cell count assay. Optimal concentrations of mitogens and incubation periods required for maximal responses to mitogens differed between the MTT assay and the viable cell count and thymidine uptake assays. This probably reflects differences in the activities measured by the MTT assay which detects mitochondrial enzyme activity, and the thymidine uptake and viable cell count assays which detect cellular proliferation activity. The validity of the MTT assay was supported by the observation that the mitogen‐induced increase in succinate dehydrogenase activity paralleled the level of mitogen‐induced MTT formazan production. Mitogen concentrations inducing maximal formazan formation in chicken splenocytes were higher than those for chicken peripheral blood lymphocytes reported previously. Results of the present study indicate that mitogen‐induced chicken splenocyte activation could be measured by the MTT colorimetric assay, although mitogen concentrations and incubation periods required for maximal splenocyte activation differed between the MTT assay and the other two assays used in this study. 相似文献
5.
The Israeli vaccine strain of fowl poxvirus grows efficiently in chicken embryo fibroblasts but not in cell lines derived from monkey kidney or human fibroblasts. We developed two assays for the titration of the infectivity of this virus in secondary cultures of chicken embryo fibroblasts. The first is a focus assay, in which minimum essential medium and SeaKem ME agarose were used for the overlay media. Under these conditions, clear virus foci appeared after 5 days of incubation at 37 C. The second assay is a semiautomatic colorimetric test based on the ability of live cells in culture to reduce the yellow tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT; thiazolyl blue) to its formazan derivative. The reagent was added to infected chicken embryo fibroblasts in 96-well plates 10 days after infection. The formazan formed during 2 hr was extracted with dimethyl sulfoxide, and its absorbance was read by an automatic microplate spectrophotometer. A good correlation of the infectivity titers of the virus was obtained by the two methods. 相似文献
6.
The (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) colorimetric assay for the quantitation of Actinobacillus pleuropneumoniae cytotoxin. 
下载免费PDF全文
下载免费PDF全文 W B Chung L R Bckstrm J McDonald M T Collins 《Canadian journal of veterinary research》1993,57(3):159-165
Using swine neutrophils as target cells, two MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) colorimetric assay systems, one with and one without phorbol 12-myristate 13-acetate (PMA) stimulation were established for the quantitation of Actinobacillus pleuropneumoniae cytotoxin. The MTT assays were optimized for the number of neutrophils, incubation time, and PMA concentration by a series of experiments. The optimal conditions were 25 x 10(4) cells/well incubated for four hours for the assay system without PMA stimulation, and 12.5 x 10(4) cells/well incubated for two hours for the assay system with PMA stimulation. One culture supernatant of a toxigenic Pasteurella multocida strain and five A. pleuropneumoniae cytotoxin preparations produced from three A. pleuropneumoniae strains were used to test assay reproducibility. Results showed both assays were reproducible with a coefficient of variation ranging from 7.8 to 18% for the assay system without PMA stimulation and from 10.7 to 18.2% for the assay system with PMA stimulation. The PMA-stimulated assay had 40 to 60-fold higher sensitivity than the nonstimulated MTT assay. The MTT assay also was applied to the measurement of neutralizing antibody titers against A. pleuropneumoniae cytotoxin. 相似文献
7.
Shkap V Pipano E Rasulov I Azimov D Savitsky I Fish L Krigel Y Leibovitch B 《Veterinary parasitology》2003,115(3):247-255
A field isolate of Theileria annulata (Uzbek strain) was obtained from calves infected by Hyalomma anatolicum ticks collected from an endemic region in Uzbekistan. Schizont-infected bovine cells that had been established and propagated in cell culture were examined for attenuation both in vivo, by inoculating cells from various passages into calves, and in vitro for metalloproteinase activity. During serial subcultivation a gradual reduction in virulence and in enzyme activity in cells infected with the Uzbek strain were observed. Complete attenuation of the Uzbek isolate was obtained at about passage 80, and only traces of proteolysis were detected in gelatin substrate gels. In contrast, there was no direct correlation between virulence and enzyme levels in an Israeli strain. While schizonts of the Israeli strain were completely attenuated at passage 80, proteolysis in the substrate gels was detected up to passage 197. Solid immunity was observed in calves immunized with attenuated T. annulata schizonts of the Uzbek strain upon challenge with the homologous H. excavatum sporozoites. For a strain to be used for vaccine production, it appears that animal inoculation still remains the most reliable method to assess the degree of attenuation and protection. 相似文献
8.
以分离自传统发酵乳制品中的4株菌——Lactobacillus paracasei supsp.paracasei M5AL、L.paracasei supsp.paracasei J23ANL、L.paracasei supsp.paracasei G15AL、L.coryniformis supsp.torquens T3AL为实验菌株,采用噻唑兰法研究其热灭活菌体、细胞壁及DNA对结肠癌细胞HT-29增殖的影响。结果表明：菌株J23ANL、M5AL、G15AL、T3AL热灭活菌体细胞、菌体细胞壁和菌体DNA均可不同程度地抑制人结肠癌细胞HT-29的增生,其中菌株T3AL对HT-29细胞增生的抑制率显著高于其他菌株的抑制率（P〈0.05）;单细胞凝胶电泳结果显示菌株T3AL可诱导HT-29细胞凋亡。 相似文献
9.
After the successful use of 3-[4,5-(dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide (MTT) in cell proliferation assays, its use has been established by different workers in cytotoxicity assays and research on leukaemia. In the present study, a colorimetric assay using MTT was adopted to evaluate the cytotoxic activity of chicken intestinal intraepithelial lymphocytes (iIELs), which constitute an important cellular component of the gut-associated lymphoid tissue (GALT). These iIELs are found to exhibit natural killer (NK) cell-like cytotoxic activity, which is spontaneous, non-MHC-restricted, and does not need to be primed. Hitherto, conventional chromium-release assays have been used to evaluate the cytotoxic activity of iIELs, but these assays have disadvantages such as radiation hazards and loss of the cells in washing steps. The mean percentage cytotoxic activity of chicken iIELs evaluated by the colorimetric assay was 90.37±2.53 in a group of 5-week-old chickens and 80.2±3.45 in a group of 8-week-old chickens. These findings established the successful use of a colorimetric assay using MTT for evaluating the cytotoxic activity of chickens iIELs.Abbreviations DMEM
Dulbecco's modified Eagle's medium
- DMSO
dimethyl sulphoxide
- E
effector cells
- GALT
gut-associated lymphoid tissue
- GM
growth medium
- iIELs
intestinal intraepithelial lymphocytes
- MTT
3-[4,5-(dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide
- NK cell
natural killer cell
- OD
optical density
- RPMI
Rosewell Park Memorial Institute medium
- T
target cells 相似文献
10.
[目的] 纯化猪塞尼卡谷病毒（Seneca Valley virus，SVV）SVV-CH-HB2016毒株，并制备其结构蛋白VP1、VP2和VP3的单克隆抗体。[方法] 以蔗糖密度梯度离心法纯化的SVV-CH-HB2016病毒颗粒作为抗原，免疫BALB/c小鼠，取脾细胞与骨髓瘤细胞（SP2/0）进行细胞融合。通过间接免疫荧光试验（IFA）结合间接ELISA筛选阳性细胞株，制备能特异性分泌针对结构蛋白的杂交瘤细胞株。采用Western blotting和IFA方法分别检测单克隆抗体与重组表达蛋白及天然结构蛋白的反应性，并对单克隆抗体的病毒中和保护效果进行测定。利用空斑试验和实时荧光定量PCR方法探究中和性单克隆抗体对SVV-CH-HB2016毒株吸附过程的影响，最后用抗体相加试验来分析14株单克隆抗体的抗原表位。[结果] 在蔗糖密度梯度为5%~45%（W/V）时获得了纯度较好、浓度较高的SVV-CH-HB2016毒株结构蛋白，免疫小鼠血清抗体效价均达到了1：12 800，成功制备了17株能稳定分泌特异性单克隆抗体的杂交瘤细胞株。经验证14株单克隆抗体能与重组结构蛋白发生Western blotting反应，17株单克隆抗体能与病毒发生IFA作用；2G6、4A3和4C11 3株单克隆抗体对SVV-CH-HB2016毒株感染的BHK-21细胞具有明显中和保护作用，也能有效抑制SVV-CH-HB2016毒株对293T细胞的吸附。经分析发现，除1F5与2E1、4B8与4F11外，其余10株单克隆抗体分别针对不同抗原表位。[结论] 本研究初步建立了SVV的纯化方法，制备了17株特异性针对SVV-CH-HB2016毒株的单克隆抗体，为后期进一步开展SVV全病毒灭活疫苗的研发、ELISA检测方法的建立及保护性抗原表位的鉴定奠定了基础。 相似文献
11.
为了评价硫酸化人参皂苷Rh2化学修饰前后免疫活性变化，采用MTT法及流式细胞术研究体外对ConA促鸡外周血淋巴细胞增殖的影响，采用乳酸脱氢酶释放法研究体外对鸡外周血NK细胞杀伤活性的影响。结果表明：Rh2、Rh2溶剂及硫酸化Rh2低浓度时都能促进淋巴细胞增殖，高浓度时能抑制淋巴细胞增殖，Rh2溶剂及硫酸化Rh2都显著（P〈0．05）或极显著（P〈0．01）高于同浓度的Rh2。Rh2及Rh2溶剂都能显著促进鸡外周血NK细胞杀伤活性，但Rh2极显著（P〈0．01）低于同浓度Rh2溶剂，而硫酸化Rh2表现一定促进作用。结果提示：Rb2体外能抑制免疫活性，化学修饰后的硫酸化Rh，体外能促进免疫活性。 相似文献
12.
The opsonization and lysis of different protozoa by antibodies and/or complement was followed using luminol-dependent chemiluminescence and bioluminescence. The addition of immune serum to variable antigen type populations of Trypanosoma evansi led to the specific opsonization of trypanosomes resulting in an intense metabolic activation and chemiluminescence response of phagocytic cells. In comparison to those of uninfected control mice, the phagocytosis of coccidia merozoites by spleen cells from mice infected with Eimeria falciformis was enhanced during the acute stage of a primary infection. Opsonizing activity was demonstrated in phosphate-buffered saline extracts of gut contents of mice infected for 10 days. The incubation of E. falciformis merozoites together with guinea-pig complement resulted in slow lysis of the cells. The addition of mouse serum collected greater than 6 days after an infection led to an accelerated lysis of the merozoites, indicating the appearance of complement-fixing antibodies in the serum. Heat-inactivated immune serum alone had no lysing activity on merozoites. In the presence of complement, bovine lymphoblastoid cells infected with Theileria annulata were lysed by anti-lymphoblastoid cell serum raised in mice but not by serum from cattle which had developed immunity to Theileria annulata. 相似文献
13.
Richardson J.O. Forsyth L.M.G. Brown C.G.D. Preston P.M. 《Veterinary research communications》1998,22(1):31-45
The proliferation of Theileria annulata macroschizont-infected cell lines in vitro was significantly inhibited by nitric oxide (NO) generated by S-nitroso-N-acetyl-DL-penicillamine (SNAP). Incubation with SNAP caused the macroschizonts to disappear and host cells to become apoptotic. SNAP-derived NO also significantly inhibited the incorporation of tritiated thymidine by cultures of cells in which the schizonts had been induced to differentiate into merozoites by maintenance at 41°C instead of 37°C, the temperature used for culturing macroschizont-infected cells. These results point to NO as the mediator of macrophage anti-T. annulata activity and provide new evidence that the protective immune mechanisms which allow cattle to recover from primary infection and resist challenge may be attributed principally to the products of activated macrophages. These findings indicate that effective inactivated vaccines against T. annulata should include antigens able to stimulate the type of CD4+ T cell response which elicits macrophage activation and NO synthesis. 相似文献
14.
E A Innes P Millar E J Glass C G Brown R L Spooner 《Research in veterinary science》1989,46(3):367-374
Bovine alloreactive cytotoxic lymphocyte (CTL) lines of known target specificity were infected in vitro with sporozoites of Theileria annulata and T parva and cultured in limiting dilution. The phenotypes of the CTL lines both pre- and post infection were assessed using a panel of monoclonal antibodies specific for defined bovine lymphocyte subpopulations. The effector function of the resultant infected cell lines was determined using a Cr51 release assay and compared to the uninfected control CTL line. The results indicated that T parva sporozoites consistently infected and transformed the CTL lines very efficiently even at the lowest cell doses. In contrast the T annulata sporozoites were largely unable to infect and transform the alloreactive CTL except at the very highest cell and sporozoite doses. A factor which appeared to influence susceptibility to T annulata infection was an increased level of class II expression on the CTL line. None of the cell lines showed cytotoxic effector function after infection with either T annulata or T parva sporozoites. 相似文献
15.
An evaluation of the mitogenic reactivity of intestinal intraepithelial lymphocytes of chickens. 总被引：1，自引：0，他引：1
A colorimetric assay employing MTT (3-[4,5-dimethylthiazole-2-yl], 2-5-diphenyltetrazolium bromide) was used to determine the mitogenic response of intestinal intraepithelial lymphocytes (i-IELs) of chickens to T- and B-cell mitogens. Comparisons between mitogenic responses of i-IELs and peripheral blood lymphocytes (PBLs) were made to examine potential relationships. The results from this study indicated that T-cell mitogens, concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) induced mitogenic stimulation in i-IELs. Although stimulation indexes of both i-IELs and PBLs were similar, the optical densities (ODs) of i-IEL cultures containing Con A or PHA-P were 20- to 50-fold lower than the ODs of PBL cultures containing the same mitogen. The lower conversion of MTT to formazan resulting in lower ODs in i-IEL cultures indicated a lower level of cellular activity in the i-IELs than in the PBLs. The mitogenic responses of both i-IELs and PBLs to Con A and PHA-P were dose dependent. The responsive concentration of Con A for i-IELs was within the range of 25-50 micrograms/ml, whereas the responsive concentration of PHA-P for i-IELs was 50 micrograms/ml. Three days of incubation was found to be adequate to induce a significant (P < 0.05) mitogenic response for both T-cell mitogens. Lipopolysaccharide was unable to induce a mitogenic response in i-IELs, which was attributed to the lack of B cells in the i-IEL population. This technique may prove useful in evaluating and studying the role of i-IELs in local cell-mediated immune responses of the gastrointestinal tract. 相似文献
16.
为建立能表达人白介素-28A(hIL-28A)基因的稳定转化家蚕卵巢细胞(BmN)系,构建了重组表达载体pIZT/V5-His-hIL-28A并转染BmN细胞,采用终浓度为300～400μg/mL的博莱霉素(zeocin)筛选2个月后,获得了稳定转化BmN细胞系。SDS-PAGE和Western blotting检测显示在稳定转化BmN细胞中表达的重组hIL-28A蛋白分子质量约为26kD;用ELISA试剂盒测定hIL-28A的表达水平约为2.035×10-5ng/个细胞。用噻唑蓝法(MTT法)检测hIL-28A的体外抗肿瘤活性,结果显示其对肺癌细胞A549、急性早幼粒细胞白血病细胞HL60、肝癌细胞BEL-7402和乳腺癌细胞M231的半数抑制浓度(IC50)分别为3.21、2.84、6.29和9.32ng/mL。研究结果表明hIL-28A可在稳定转化BmN细胞中表达,表达产物具有体外抗肿瘤活性。 相似文献
17.
Gerardo Iglesias Carlos Pijoan Thomas Molitor 《Comparative immunology, microbiology and infectious diseases》1992,15(4):249-259
Alveolar macrophages (AM) infected with Pseudorabies virus (PRV) were compared to noninfected AM for cytotoxicity against foreign or transformed cells and production of interferon (IFN). Five PRV strains were used to infect AM including strains that are known to be highly virulent for pigs, i.e. strain 4892 and strain S-62 as well as strains that are regarded as mild or nonvirulent, i.e. BUK and Bartha. The multiplicity of infection ranged from 0.005 to 0.05 TCID50/cell. The target cells in the cytotoxicity assays were either chicken red blood cells, PRV-infected vero cells, or human myeloblastoma cells (K562 cell line). For the producton of IFN, AM cultures were treated with polyinosinic: polycytidylic acid (Poly I:C) diluted in tissue culture media at a concentration of 5 μg/106 cells. Culture supernatants were collected at various times poststimulation and tested for antiviral activity using the Vesicular Stomatitis Virus replication inhibition test. Swine AM were able to lyse chicken red blood cells in an antibody-independent way but not in an antibody-dependent way, whereas lysis of PRV-infected vero cells was accomplished both ways. The cytotoxicity against chicken red blood cells was reduced in the PRV-infected AM as compared to noninfected cells, particularly in AM infected with virulent PRV strains. Specific 51Cr release values for AM infected with S-62 and 4892 strains were 14 and 19, while the noninfected AM had values of 36. Similarly, in the antibody-dependent cytotoxicity assay against PRV-infected vero cells there was no activity of AM against K562 cells. The production of IFN was readily stimulated with Poly I:C. The optimal time for supernatant collection was between 12 and 16h poststimulation. The antiviral activity was abrogated by treatment of the supernatant with antiserum against human leukocyte IFN; it was therefore considered to be due to interferon-alpha (IFN) released from the macrophages. The antiviral activity present in supernatants of PRV-infected AM was reduced compared to noninfected AM. The difference between AM cultures infected with virulent strains of PRV and noninfected AM cultures was statistically significant at P 0.025. The results provide support to the premise that the role of AM in lung defense can be compromised by PRV infection. 相似文献
18.
OBJECTIVE: To develop an in vitro fluorometric assay to assess Pasteurella haemolytica adherence to bovine respiratory and epithelial cells and compare adherence of single strains of P. haemolytica serovars A1 and A2 (PhA1 and PhA2, respectively). SAMPLE POPULATION: Monolayers of bovine turbinate and Madin Darby bovine kidney (MDBK) cells. PROCEDURE: To determine optimal inoculum concentration and incubation time, various concentrations of P. haemolytica were labeled with fluorescein isothiocyanate and incubated with monolayers of bovine cells for various times. Bovine cells were washed to remove nonadherent bacteria, and percentage of bacteria adhered (percentage of adherence) was estimated fluorometrically. Percentage of adherence of PhA1 was compared with that of PhA2. RESULTS: The optimal inoculum concentration that resulted in measurable fluorescence of adherent bacteria was 1 x 10(8) colony-forming units/ml, and the optimal incubation time was 45 minutes. Percentage of adherence of PhA1 to MDBK and turbinate cells was significantly greater than that determined for PhA2. CONCLUSIONS: The in vitro fluorometric assay is a time-efficient, inexpensive, and labor-saving method for evaluation of P. haemolytica adherence to bovine cells. The concentration of bacteria used to inoculate bovine cells in this assay is similar to that typically used in other types of in vitro adherence assays. The predominance of PhA1 over PhA2 during the early stages of bovine respiratory disease may be attributable to the ability of PhA1 to adhere more avidly to nasopharyngeal tissue. 相似文献
19.
The interactions of 2 capsular serotype A and 4 serotype D strains of Pasteurella multocida with rabbit polymorphonuclear neutrophils (PMN) were compared in vitro, using a PMN phagocytic and bactericidal assay. Bacteria and rabbit PMN were incubated for 15 minutes. The suspensions were subjected to differential centrifugation and the percentage of phagocytosis (cell association) was determined from the number of viable noncell-associated bacteria. The cell pellets and the associated bacteria were resuspended and PMN bactericidal activity was calculated from the number of remaining viable cell-associated bacteria at 45 and 75 minutes after the start of the assay. Test bacteria were not opsonized or were opsonized with immune serum containing active complement. One type A strain was ingested and killed by PMN in the presence and absence of opsonins. The 5 remaining strains were resistant to PMN killing, but only the type A strain resisted phagocytosis. Resistance of the type A strain was attributed to the hyaluronic acid capsule, since pretreatment of the bacteria with hyaluronidase rendered opsonized bacteria susceptible to ingestion and killing. The pattern of resistance of the 4 type D strains was different from that of the resistant type A strain. Both opsonized and nonopsonized type D bacteria became cell associated, but none were killed by PMN. The mechanism of resistance of these 4 strains to PMN bactericidal activity is currently unknown. 相似文献
20.
T. R. Melrose C. G. D. Brown S. P. Morzaria J. G. R. Ocama A. D. Irvin 《Tropical animal health and production》1984,16(4):239-245
Six stocks of Theileria annulata isolated from the Sudan and nine stocks of T. parva, isolated in Kenya and Malawi were grown in bovine lymphoblastoid cell lines. Lysates prepared from the infected cultures were examined electrophoretically on thin layer starch gels for evidence of glucose phosphate isomerase polymorphism. The six stocks of T. annulata showed major variations in their parasite enzyme patterns but no variation was detected in nine stocks of T. parva. 相似文献