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为了考察戊二醛苯扎溴铵溶液、复方戊二醛溶液2种消毒剂对猪舍自然菌的现场消毒效果,参考中华人民共和国农业部《兽用消毒剂技术规范》(1992年版),采用戊二醛苯扎溴铵溶液100倍稀释、复方戊二醛溶液150倍稀释,通过擦拭法进行消毒前后表面菌落计数,计算杀菌率,判定其现场消毒效果。结果显示:戊二醛苯扎溴铵溶液100倍稀释,复方戊二醛溶液150倍稀释时,与猪舍自然菌作用60 min,杀菌率达96.45%以上。结果表明:2种消毒剂对猪舍自然菌消毒效果相当,戊二醛苯扎溴铵溶液可以推广使用。 相似文献
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进行了奶牛乳头药浴碘甘油溶液的杀菌作用研究。试验表明,当给药浓度为0．25％（含碘）时,对大肠杆菌、金黄色葡萄球菌和溶血性链球菌的最短作用时间为15s,杀菌率均为100％,对白色念珠菌作用的最短时间为1min,杀菌率为100％。结果说明该消毒剂对上述细菌和真菌均具有良好的杀灭作用,可用于奶牛挤奶前后乳头的药浴消毒,推荐临床药浴使用浓度为0．25％f原液作1：8倍稀释）． 相似文献
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“菌毒净”主要成分是三氯异氰脲酸，其有效氯含量比市场上常见的二氯异氰脲酸制剂高25％，消毒效果好，本试验表明，“菌毒净”按1：750和1：1500倍稀释用于场地消毒，作用30min以后，其杀菌率均达到99．99％以上。 相似文献
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过硫酸氢钾复合粉消毒作用试验研究 总被引：2，自引：0，他引：2
采用纸片琼脂扩散、定量和定性的杀菌试验方法,对过硫酸氢钾复合粉的消毒效果进行研究。结果显示,0.125%-1%的过硫酸氢钾复合粉消毒剂对大肠杆菌8099、金黄色葡萄球菌ATCC6538、多杀性巴氏杆菌C48-1、链球菌C55100均有抑菌作用,对前三种菌的最低杀菌浓度为0.25%,对链球菌C55100的最低杀菌浓度为0.125%。对受试菌作用10 min,杀菌率均为100%;作用5 min,杀菌率也可达到99.9%以上,符合杀菌率要求。 相似文献
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《广东畜牧兽医科技》2021,46(4)
本试验采用定量杀菌和现场消毒评价5%聚维酮碘溶液定量杀灭细菌繁殖体和现场消毒的效果。对于细菌繁殖体,5%的聚维酮碘溶液分别进行1︰3500和1︰6000稀释度时,室温条件下(20℃左右)作用5 min,其杀灭率均达99.90%以上。现场消毒结果表明,无论是地面、墙面、圈槽、空气消毒,5%聚维酮碘溶液高浓度(1︰200)、中浓度(1︰300)消毒10 min,杀菌率均可达99.90%以上。 相似文献
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猪小肠抗菌肽的抗菌作用研究 总被引：26，自引：0，他引：26
采用琼脂糖弥散试验和活菌计数试验检测猪小肠抗菌肽对儿株细菌(鸡大肠杆菌(O1)C83845株、鸡白痢沙门氏菌C79-13株、大肠杆菌(O2)、大肠杆菌(O78)、猪致病性大肠杆菌自然分离株、鸡致病性大肠杆菌自然分离株、白色葡萄球菌、金黄色葡萄球菌ATCC25923株、猪致病性沙门氏菌自然分离株、绿脓杆菌ATCC27853株、鱼致病性嗜水气单胞菌)的作用。结果表明，猪小肠抗菌肽对以上各株细菌均有不同程度的抑制作用，杀菌率由59．5％～98．5％不等。扫描电镜下观察猪小肠抗菌肽分别与鸡大肠杆菌(O1)C83845株、鸡白痢沙门氏菌C79-13株和金黄色葡萄球菌ATCC25923株在37．0℃条件下共培养15min后细菌形态学的改变，结果发现，有的细菌表面出现囊泡样或不规则突起样结构；有的细菌严重变形、扭曲，一端呈火柴头状；有的细菌表面变得粗糙、皱缩、胞膜破裂、内容物外流而死亡。 相似文献
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目的观察微电流对水体中细菌的杀灭效果,为养殖业污水消毒寻找新方法提供理论支撑。方法通过开展悬液定量杀菌试验、影响因素测定试验和模拟现场试验,对微电流杀灭大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和枯草芽孢杆菌的效果进行研究,探讨温度和pH值对消毒效果的影响,并以人工湖水和养殖场污水为消毒对象进行模拟现场试验。结果微电流消毒作用4 min时,对4种菌悬液的杀灭对数值均>5.0。温度和pH值对微电流消毒效果无影响。利用微电流对人工湖水和养殖场污水进行消毒,作用4 min时杀菌率达到99.99%,水中细菌基本已被杀灭;作用5 min时,水中细菌菌落总数下降至0 CFU/mL,能达到完全杀灭细菌的效果。结论微电流对畜禽养殖污水中常见细菌具有杀灭作用,在畜禽养殖业消毒方面具有广阔的应用前景。 相似文献
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为评价泡沫型消毒剂的实际消毒效果，特进行现场消毒试验。选取一栋空置猪舍，使用泡沫枪以泡沫型戊二醛癸甲溴铵溶液消毒，分别对地面、墙壁、房顶各区域3处消毒前后的杀菌率进行计算。结果显示，9处检测点的杀菌率均大于98.00%，证实泡沫型消毒剂对水平面和垂直面均可以起到较好的消毒效果。 相似文献
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采用Klein-Defors悬浮杀灭与感染试验方法，研究“卫可”、“卫康”两种消毒剂在不同浓度下，分别与流感H51N1和H9N2亚型病毒作用5min和10min，对流感病毒的灭活作用。结果表明：这两种消毒剂在相同或不同的浓度范围内。对病毒的杀灭率是有区别的。尽管每种消毒剂在本身稀释浓度范围内都有较好的杀毒效果，但在应用中也应考虑其他因素的影响。因此，我们推荐使用该消毒剂的工作浓度为100％杀灭病毒的稀释度。这将为其临床应用提供依据，对环境消毒和防止流感爆发具有参考价值。 相似文献
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优氯净对苏云金杆菌伴孢晶体消毒效果及机理的研究 总被引：1，自引：1，他引：0
苏云金杆菌(Bacillus thuringiensis,简称BT菌)的伴孢晶体被优氯净液处理后,在相差显微镜下观察,失去暗蓝色光泽,呈暗黑色,难溶于碱性缓冲液。虽能溶于昆虫的胃液,却失去致病性。用扫描电镜和SDS—聚丙烯酰胺凝胶电泳进一步研究,结果表明:优氯净能破坏伴孢晶体蛋白的一级和立体结构,使晶体蛋白失活,达到消毒的目的。消毒能力测定结果表明:1毫升0.03％有效氯优氯净液在3分钟内能消毒50微升BT菌液(3μg伴孢晶体/μl菌液)。消毒BT菌污染桑叶,用0.01％有效氯优氯净液浸泡3分钟即可。考虑到生产上的安全性,我们建议用0.05％有效氯优氯净消毒3—5分钟。 相似文献
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为了观察“二氧化氯泡腾片”对大肠杆菌、金黄色葡萄球菌、禽流感H9亚型病毒及鸡新城疫病毒的杀菌及杀病毒效果,采用悬液定量杀菌试验和通过固定病毒稀释消毒液法进行实验室观察。结果：二氧化氯5mg／L作用5min即可对大肠杆菌临床分离株产生t00％的杀菌效果。二氧化氯5mg／L作用10min或二氧化氯10mg／L作用5min均能对金黄色葡萄球菌临床分离株产生100％的杀菌效果。2mg／L二氧化氯就能对10砸ID50新城疫病毒有100％的杀灭作用,而1mg／L不能全部杀灭新城疫病毒。禽流感病毒H9亚型对二氧化氯比新城疫病毒敏感,1mg／L二氧化氯即可对10^3EID50禽流感病毒H9有较好的杀灭作用。结论：“二氧化氯泡腾片”是一种方便、高效的消毒剂,在较低的浓度条件下,就能对家禽主要病原微生物有杀灭效果。 相似文献
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蚕用消毒剂亚迪欣的产品质量检测分析 总被引：2，自引：0，他引：2
亚迪欣是一种新型蚕用消毒药剂,主要有效成分为三氯异氰尿酸、磷酸三钠。为加强该消毒药剂的生产质量管理,进行了产品的质量检测分析试验。药剂中三氯异氰尿酸的有效氯含量检测方法经对照试验、精密度及重复性试验,其相对标准差(RSD)分别为0.02%、0.10%及0.12%,且在0.418~2.338 mg/mL范围内线性关系良好,R2=1;加入磷酸三钠的平均回收率为100.1%、RSD=0.16%,说明加入磷酸三钠对三氯异氰尿酸的有效氯含量检测没有影响。磷酸三钠在密塞条件下放置24 h检测其含量基本不变,在干燥条件下的质量分数增加1.08%,在潮湿条件下的质量分数减少1.41%。试验结果显示对亚迪欣产品有效成分含量的检测方法及标准制订合理可靠,产品质量可控性强。 相似文献
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Five disinfectants, Venno FF super, Venno Vet 1 super, Venno Oxygen, M&Enno-Veterin?r B neu und Neopredisan 135-1, were tested to evaluate their efficacy against caliciviruses at 20 and 10 degrees C. As model test virus served feline calicivirus type F9 (FCV F9). All disinfectants were tested according to Guidelines of the German Veterinary Association (DVG). The investigations were performed in suspension tests and germ carrier tests. The suspension tests were carried out without and with protein load. As protein was used foetal calf serum at the concentration of 40%. Venno FF super showed less protein dependence, however a considerable temperature dependence. This matter can be corrected by increase of concentration on 2%. Venno Vet 1 super was without protein especially effective. The losses on the effectiveness through low temperature and protein load can be annulled also here by increase of concentration. Venno Oxygen was more effective in the comparison to that here named both preparations. The effects of temperature can be corrected by extension of reaction time. The most effective preparation was M&Enno Veterin?r B neu. The disinfection occurred at 20 degrees C with 0.5% solution within 120 min and at 10 degrees C with 1.0% solution within 60 min. The fifth disinfectant Neopredisan was in suspension tests without protein load and carrier tests with gauze at 20 and 10 degrees C relative convincing but in germ carrier tests with poplar wood, no complete disinfection could be achieved within tested concentrations and reaction times. 相似文献
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试验旨在对本实验室研发的用于奶牛乳头消毒的二氧化氯消毒剂进行实验室杀菌效果评价,并对二氧化氯的杀菌机理进行初步研究。采用定量法杀菌试验对二氧化氯奶牛乳头消毒剂进行实验室杀菌效果评价;利用透射电子显微镜观察二氧化氯作用后金黄色葡萄球菌超微结构的改变;利用流式细胞仪测定二氧化氯作用后金黄色葡萄球菌细胞膜通透性的变化。定量法杀菌试验结果显示,当该消毒剂中二氧化氯的含量高于1.2 mg/L时,对无乳链球菌、铜绿假单胞菌的杀灭对数值高于3.00,即杀菌率高于99.9%;二氧化氯含量高于0.6 mg/L时,对金黄色葡萄球菌、大肠杆菌和白色念珠菌杀灭对数值高于3.00,即杀菌率高于99.9%;二氧化氯的含量为0.3 mg/L时,仍能有效杀灭表皮葡萄球菌、停乳链球菌;而二氧化氯含量高于28 mg/L,对于枯草芽孢杆菌的杀菌率才能达到100%。透射电子显微镜超微结构观察结果显示,100 mg/L二氧化氯作用于金黄色葡萄球菌5 min时,细胞膜出现了轻微的皱缩,细胞壁与胞膜之间出现了轻微的空隙,绝大多数细胞仍保持原有形态;作用15 min后,金黄色葡萄球菌形态发生了较明显的变化,细胞膜发生了明显的皱缩,细胞壁与细胞膜完全脱离,细胞质也出现了凝集。流式细胞仪检测金黄色葡萄球菌细胞膜通透性变化发现,二氧化氯处理后金黄色葡萄球菌细胞膜通透性发生了改变,其中含量为50 mg/L二氧化氯作用15 min后细胞膜通透性改变最明显。结合实验室杀菌效果评价结果发现,二氧化氯对细菌细胞膜通透性的改变仅仅是致死细菌的原因之一,二氧化氯对细菌的杀灭机理较复杂,仍需进一步的探索。 相似文献
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Gutiérrez CB Alvarez D Rodríguez-Barbosa JI Tascón RI de la Puente VA Rodríguez-Ferri EF 《American journal of veterinary research》1999,60(4):481-484
OBJECTIVE: To study the efficacy of N-duopropenide against various gram-positive and gram-negative organisms. SAMPLE POPULATION: One field strain each of Pasteurella multocida subsp multocida, Staphylococcus aureus, Listeria monocytogenes, and L ivanovii, and 2 field strains of Escherichia coli. PROCEDURE: Strains were tested with and without serum as organic matter, using quantitative suspension and carrier tests. Six concentrations of active ingredient (0.005, 0.01, 0.05, 0.11, 0.27, and 0.55%) and 6 contact times (15 and 30 seconds and 1, 3, 5, and 10 minutes) were studied for each. RESULTS: Globally, N-duopropenide was more effective in suspension tests than in carrier tests, and when organisms were suspended in saline solution rather than serum. Under the most disadvantageous conditions (carrier test with serum), a concentration of 0.55% N-duopropenide acting for only 15 seconds was effective in inactivating P multocida subsp multocida and was even more effective against the 2 Listeria species tested. For E coli strains, the same concentration also was effective, but after 10 minutes of contact. On the other hand, N-duopropenide was unable to inactivate the S aureus strain in the carrier test with serum, a concentration of 0.55% for 10 minutes was necessary to inactivate it without organic matter; however, N-duopropenide was highly effective against this organism in the suspension test, even with serum. CONCLUSION: N-duopropenide was highly effective in vitro against 5 of the most commonly encountered organisms in clinical veterinary medicine and, consequently, might be a good choice in control measures against common pathogenic organisms in modern production systems. 相似文献
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The objective of this study was to evaluate the relative efficacy of otoscope cone cleaning and disinfection methods commonly used in veterinary practices. Using sterile technique, 60 new gas-sterilized 4-mm otoscope cones were inoculated with a broth culture of 1.5 billion Pseudomonas aeruginosa bacteria per mL then allowed to dry for 10 min. Six study groups of 10 cones each were created. Group 1 served as positive control and received no cleaning or disinfection. Group 2 cones were wiped with sterile cotton-tipped applicators and gauze then rinsed with water. Group 3 cones were wiped with 70% isopropyl alcohol. Group 4 cones were scrubbed in a speculum cleaner with Cetylcide II solution (Cetylite Industries, Inc., Pennsauken, NJ). Groups 5 and 6 cones were soaked for 20 min in Cetylcide II and chlorhexidine gluconate 2% solutions, respectively. Using sterile technique and after 10-15 min drying time, the cones were swabbed in a consistent pattern, and samples were submitted for quantitative culture. Culture results showed no growth from cones soaked in Cetylcide II or chlorhexidine solutions. Two of the 10 cones wiped with alcohol, 3/10 cones wiped then rinsed with water, and 3/10 cones scrubbed with the speculum cleaner showed growth of P. aeruginosa. All (10/10) cones in the control group showed heavy growth of P. aeruginosa. These results show that P. aeruginosa can survive on otoscope cones cleaned and disinfected by several commonly used methods. Further study is needed to determine practical and optimal cleaning and disinfection methods for otoscope cones. 相似文献