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1.
Abstract. The saline–sodic soils of the dryland Songnen Plain in northeast China are only slowly permeable to fresh water because of their large content of montmorillinite clay and sodium bicarbonate. Use of slightly saline groundwater containing adequate dissolved calcium and magnesium for leaching and reclamation can potentially prevent dispersion of the clay soil particles during treatment. Amelioration was evaluated using shallow, mildly saline groundwater to irrigate sorghum–corn rotations in a two-year field experiment. After two growing seasons during which a total of 400 mm of leaching water was applied, in addition to some supplemental irrigation water, the average electrical conductivity (ECe) of the top 1.2 m of the soil profile decreased from 14.5±3.5 to 2.7±0.2 dS m−1, and the sodium absorption ratio (SARe) decreased from 35.3±4.1 to 10.1±2.5 (meq L−1)0.5. The soil physical properties were improved: infiltration rate with mildly saline groundwater increased from 12.1 to 42 mm h−1. Salinity changes in the top 1.2 m of soil layers after 700 mm of leaching produced no further improvement. Crop yields produced on plots undergoing amelioration increased by 64–562% compared with the rainfed control. The improved soil conditions after leaching resulted in 59–548% greater crop yields.  相似文献   

2.
Peat land has been considered as an alternative type of land for agricultural development especially in the tropics. In the present study, the N-supplying capacity, one of the most important soil properties in terms of crop production, of peat soils was examined. Ten peat soil samples were collected from Indonesia, Malaysia, and Japan. Gross N mineralization in the soil samples was estimated using a zero-order model, and kinetic parameters of mineralization were determined using a simple type model. Soil organic matter composition was investigated using 13C CPMAS NMR. Mineralization potential ( N 0), apparent activation energy ( E a), and mineralization rate constant ( k ) ranged between 571–2,445 mg kg−1, 281–8,181 J mol−1, and 0.009–0.020 d−1, respectively. Although none of the parameters showed a significant correlation with the soil C/N ratio, a negative correlation was observed between the k value and the ratio of the proportion of alkyl C in total C to that of O -alkyl C estimated by 13C CPMAS NMR. The latter suggested that the k values were higher in the peat soils relatively rich in readily decomposable organic matter including carbohydrates.  相似文献   

3.
Emissions of nitrous oxide (N2O) and nitrogen gas (N2) from denitrification were measured using the acetylene inhibition method on drained and undrained clay soil during November 1980-June 1981. Drainage limited denitrification to about 65% of losses from undrained soil. Emissions from the undrained soil were in the range 1 to 12 g N ha–1 h–1 while those from the drained soil ranged from 0.5 to 6 g N ha–1 h–1 giving estimated total losses (N2O + N2) of 14 and 9 kgN ha–1.
Drainage also changed the fraction of nitrous oxide in the total denitrification product. During December, emissions from the drained soil (1.8±0.6 gN ha–1 h–1) were composed entirely of nitrous oxide, but losses from the undrained soil (2.7 ± 1.1 g N ha–1 h–1) were almost entirely in the form of nitrogen gas (the fraction of N2O in the total loss was 0.02). In February denitrification declined in colder conditions and the emission of nitrous oxide from drained soil declined relative to nitrogen gas so that the fraction of N2O was 0.03 on both drainage treatments. The delayed onset of N2O reduction in the drained soil was related to oxygen and nitrate concentrations. Fertilizer applications in the spring gave rise to maximum rates of emission (5–12g N ha–1 h–1) with the balance shifting towards nitrous oxide production, so that the fraction of N2O was 0.2–0.8 in April and May.  相似文献   

4.
Abstract. Nitrate leaching and soil mineral N status under grassland were measured on three contrasting soils, spanning winters 1995/96, 1996/97 and 1997/98, in Western England. The soils investigated were a freely draining silty clay loam (Rosemaund), a well drained loam (IGER 1) and a poorly drained clay loam (IGER 2). The effects of reseeding (ploughing and resowing grass) at IGER 1 and IGER 2 in autumn 1995 or 1996 were compared with undisturbed pasture. Reseeding at Rosemaund, in autumns 1995 or 1996, or spring 1996 was compared with undisturbed pasture of 3 sward ages (2, 5, >50 years).
Nitrate-N leaching losses during the winter immediately following autumn reseeding ranged between 60 and 350 kg N ha–1 in 1995/96, depending on soil type, sward management history and rainfall. Losses were much less in the following winter when treatments were repeated (10–107 kg N ha–1).
Reseeding in spring had little effect on soil mineral N content or leaching losses in the following autumn, compared with undisturbed pasture. Similarly, leaching losses from autumn reseeds in the second winter after cultivation were the same as undisturbed pasture (1-19 kg N ha–1). The effect of ploughing grassland for reseeding was relatively short-term, in contrast to the effect of repeated annual cultivation associated with arable rotations.  相似文献   

5.
Real-time images of nitrogen fixation in an intact nodule of hydroponically cultured soybean ( Glycine max [L] Merr.) were obtained. In the present study, we developed a rapid method to produce and purify 13N-labeled radioactive nitrogen gas (half life: 9.97 min). 13N was produced from a 16O (p, α) 13N nuclear reaction. The target chamber was filled with CO2 and irradiated for 10 min with protons at an energy of 18.3 MeV and an electric current of 5 μA, which was delivered from a cyclotron. All CO2 in the collected gas was absorbed and removed with powdered soda-lime in a syringe and replaced with helium gas. The resulting gas was injected into gas chromatography and separated and a 35 mL fraction, including the peak of [13N]-nitrogen gas, was collected by monitoring the chromatogram. The obtained gas was mixed with 10 mL of O2 and 5 mL of N2 and used in the tracer experiment. The tracer gas was fed into the underground part of intact nodulated soybean plants and serial images of the distribution of 13N were obtained non-invasively using a positron-emitting tracer imaging system (PETIS). The rates of nitrogen fixation of the six test plants were estimated to be 0.17 ± 0.10 μmol N2 h−1 from the PETIS image data. The decreasing rates of assimilated nitrogen were also estimated to be 0.012 ± 0.011 μmol N2 h−1. In conclusion, we successfully observed nitrogen fixation in soybean plants with nodules non-invasively and quantitatively using [13N]N2 and PETIS.  相似文献   

6.
At Onne in South-east Nigeria, drainage water was collected from four monolith lysimeters and analysed for nitrate. The lysimeters contained an acid sandy loam. At the start of the first rainy season two lysimeters received urea labelled with 15NO3 and two received no nitrogen fertilizer; all four were uncropped in the first year.
The peak concentrations of 15NO3 and of unlabelled (soil) NO3 were found after 2.5 pore volumes of water had passed through the lysimeters. Using the same soil in the laboratory after fine sieving, the peak concentration of tritiated water was found at 1 pore volume whereas nitrate leaching was retarded. The pattern of nitrate leaching was well described by miscible and immiscible models which included an adsorption coefficient for nitrate. Over the 2 years 81.4% of the 15N added at the start of the first rainy season was recovered in the drainage water.  相似文献   

7.
The β-ketoadipate pathway is the major route for degradation of aromatic compounds by various soil microorganisms. Protocatechuate 3,4-dioxygenase, a key enzyme of this pathway and which is encoded by pcaGH genes, catalyses the ring cleavage of protocatechuate. Microorganisms harbouring pcaGH genes are widely distributed in the environment but little is known about their relative abundance within the total microflora. Hence, this paper reports the development of a real-time PCR assay to quantify the bacterial pcaH sequence that encodes the β sub-unit of the protocatechuate 3,4-dioxygenase. This real-time PCR assay was linear over seven orders of magnitude with a calculated efficiency of 99% and sensitive up to 102 copies of the pcaH sequence per assay. Real-time PCR analyses performed on six soils with different physico-chemical properties, revealed pcaH densities ranging from 103 to 104 copies of pcaH  ng−1 of soil DNA, which corresponded to approximately 0.2–10.9% of the total bacterial community. The sequencing of real-time PCR amplicons yielded 48 deduced amino acid sequences that exhibited 44–100% identity to known bacterial PcaH sequences, thereby confirming the accuracy of this real-time PCR assay.  相似文献   

8.
We used a laboratory incubation approach to measure rates of net N mineralization and nitrification in forest soils from Fu-shan Experimental Forest WS1 in northern Taiwan. Net mineralization rates in the O horizon ranged from 4.0 to 13.8 mg N kg−1 day−1, and net nitrification rates ranged from 2.2 to 11.6 mg N kg−1 day−1. For mineral (10–20 cm depth) soil, net mineralization ranged from 0.06 to 2.8 mg N kg−1 day−1 and net nitrification rates ranged from 0.02 to 2.8 mg N kg−1 day−1. We did not find any consistent differences in N mineralization or nitrification rates in soils from the upper and lower part of the watershed. We compared the rates of these processes in three soil horizons (to a soil depth of 30 cm) on a single sampling date and found a large decrease in both net N mineralization and nitrification with depth. We estimated that the soil total N pool was 6,909 kg N ha−1. The present study demonstrates the importance of the stock of mineral soil N in WS1, mostly organic N, which can be transformed to inorganic N and potentially exported to surface and ground water from this watershed. Additional studies quantifying the rates of soil N cycling, particularly multi-site comparisons within Taiwan and the East Asia–Pacific region, will greatly improve our understanding of regional patterns in nitrogen cycling.  相似文献   

9.
The origin of highly acidic (pH<4.5) barren soils in the Klamath Mountains of northern California was examined. Soil parent material was mica schist that contained an average of 2,700 mg N kg−1, which corresponds to 7.1 Mg N ha−1 contained in a 10-cm thickness of bedrock. In situ soil solutions were dominated by H+, labile-monomeric Al3+ and NO3, indicating that the barren area soils were nitrogen saturated—more mineral nitrogen available than required by biota. Leaching of excess NO3 has resulted in removal of nutrient cations and soil acidification. Nitrogen release rates from organic matter free soil ranged from 0.0163 to 0.0321 mg N kg−1 d−1. Nitrogen release rate from fresh ground rock was 0.0465 mg N kg−1 d−1. This study demonstrates that geologic nitrogen may represent a large and reactive nitrogen pool that can contribute significantly to soil acidification.  相似文献   

10.
The magnitude of radiocaesium fixation by micaceous clay minerals is affected by their transformation, which depends on weathering in soil. The net retention of radiocaesium traces was quantified by sorption–desorption experiments in the various horizons of four sandy soils forming an acid brown earth–podzol weathering sequence derived from sandy sediments and characterized by marked changes in mineral composition. The features of the 2:1 minerals of the four soils, resulting from an aluminization process in depth and a desaluminization process towards the surface, had a strong influence on Cs+ fixation. Beneath the desaluminization front, which deepens from the acid brown earth to the podzol, hydroxy interlayered vermiculite was dominant and the 137Cs+ fixation was the weakest. At the desaluminization front depth, vermiculite was responsible for the strongest 137Cs+ fixation. In the upper layers, smectite appeared in the podzolized soils and the 137Cs+ fixation decreased. The magnitude in Cs+ fixation therefore appeared as a tracer of the transformation process affecting the 2:1 clay minerals in the acid brown earth–podzol weathering sequence. This magnitude was positively correlated with the vermiculite content of the studied soil materials estimated by the rubidium saturation method.  相似文献   

11.
Abstract. Regression equations successfully allowed the calculation of water retained at—0.3 × 105 Pa and–15 × 105 Pa matric potentials from single soil characteristics, such as bulk volume or clay content, in clayey horizons derived in similar ways from a single parent material. It is possible to use these regression equations on other soils with similar clay fabrics. The fabric is expressed numerically using the pore volume associated with clay particles.  相似文献   

12.
(pp. 17–24)
A trial calculation was performed of the environmental nitrogen-assimilation capacity and the amount of nitrogen input based on various statistical data, which were compiled from each city, town and village in Hokkaido prefecture. The relationship between the excess quantity of nitrogen, after nitrogen input, and the environmental nitrogen-assimilation capacity and the nitrate-nitrogen concentration of the groundwater was considered.
Environmental nitrogen-assimilation capacity = nitrogen output by the crops + acceptable level of residual nitrate in the soil profile.*
*It is calculated by the amount of nitrate precipitation evapotranspiration ×10 mg L−1.
  • 1) 

    The average value of the environmental nitrogen-assimilation capacity in Hokkaido Prefecture was observed to be 183 kg ha−1. The maximum and minimum values of the environmental nitrogen-assimilation capacity were 308 kg ha−1 and 94 kg ha−1, respectively. When the average value of the environmental nitrogen-assimilation capacity with respect to main agricultural land use was compared across municipalities, it was largely in the following order · grassland (218 kg ha−1), upland (169 kg ha−1), and paddy land (157 kg ha−1).

      相似文献   

13.
We investigate the effect of Al on K+ uptake by rice roots. Potassium-38 (38K), a positron emitting nuclide (the half-life: 7.61 min), was used to trace K+ behavior. When a rice root was treated with 10μM Al for 24 h, the uptake of 38K in the root was increased in the range of 1 to 2 cm from the root tip compared with that of the control sample. Because the root continued to grow without showing any damage of plasma membrane during the Al treatment, it was suggested that the 38K uptake was not occurred through diffusion into the cells. The uptake of 38K in both treatments, with/without Al, was decreased by VO43- (inhibitor of H+-ATPase on plasma membrane) and DNP (H+ ionophore) treatment, which suggested that the K+ uptake was performed through an active transport, such as H+:K+ transport or H+ gradient promoted by an Al treatment.  相似文献   

14.
Previous trials have revealed variable responses of sago palm ( Metroxylon sagu Rottb.) to fertilizer application, particularly nitrogen (N). In the present study, we quantified the fertilizer use efficiency (FUE) of sago palm for the first time using 15N-labeled fertilizer in pot and field experiments. The pot experiment was conducted in Japan using a 2:1 mixture of sand to Philippine soil. The field experiment was conducted in Leyte in the Philippines. Both experiments consisted of three replicates in each of three treatments: control, 15N urea at 50 kg N ha−1 and 15N urea at 100 kg N ha−1. The N uptake of sago palm increased significantly, but inconsistently with increasing N application. The few instances of a significant increase in N uptake did not translate into significant improvements in growth parameters, except for the number of leaflets in the pot experiment. The FUE values for sago seedlings (< 6 months) in the pot experiment treated with 50 and 100 kg N ha−1 were 10.5 and 13.2%, respectively, whereas for the 2-year-old sago palms in the field, the corresponding FUE values were 14.8 and 12.0%. The FUE values were similar at the two levels of N application in both experiments. Sago growth parameters appeared to be insensitive to N application, suggesting that the form of N and the timing of N fertilization are important factors for sago palms. Therefore, the use of N fertilizer in sago production can only be justified after determining and fully understanding the response of sago palm to N application.  相似文献   

15.
Abstract. 15N labelled NH4NO3 (fertilizer N) was applied at a rate of 50 kg N ha–1 to an Ando-Humic Nitisol and two maize crops grown on it. About 20 months later, soil cores were taken to a depth of 2.5 m. Leached fertilizer N was found between 1.4 m and 1.8 m deep and was delayed relative to net drainage by between 4.2 and 4.9 pore volumes. Anion exchange capacity (AEC) increased ten-fold down the profile, up to 2.9 cmolckg–1. The delay to fertilizer N leaching was predicted to be between 4.1 and 5.3 pore volumes when calculated from the AEC and from an equation relating delay due to AEC in laboratory columns of repacked soil obtained by Wong et al. (1990b). It was concluded that the nitrate leaching delay equation was also valid in undisturbed field profiles. Two concentration maxima for mineral N were found, which did not usually coincide with the fertilizer N and were thought to result from mineralization of soil organic matter and plant residues at the end of each season. The delay equation overestimated their leaching delay but the results were considered close enough to support the hypothesis for their formation.  相似文献   

16.
Summary A clear understanding of the short-term decomposition and fate of crop residues is necessary to predict the availability of mineral N in soil. The fate of 13 C15N-labelled wheat straw in a silty soil (Typic Hapludalf) was studied using particle size fractionation and in situ incubation in which the equivalent of 8 t dry matter per ha of straw was incorporated into the soil over 574 days. Soil samples were separated into five particle-size fractions by wet sieving after disruption of aggregates. The weight, C and N contents, and 13C and 15N atom excess of each fraction were determined. Straw-derived C disappeared rapidly from the > 2000-μm fraction with an estimated half-life of 53 'normalized' days (equivalent of 10°C and −0−01 MPA water potential). Straw-derived C appeared to be only temporarily stored in the intermediate fractions (1000–2000 and 200–1000 pm). The maximum net 13C accumulation in the 50–200-μm fraction was 4·4% of added 13C. Straw-derived C accumulated most rapidly and preferentially in the 50-μm fraction, which stabilized after 265 days and accounted for 70% of the residual 13C on day 574. Although there was more residual 15N than 13C, the distributions and kinetics of the two isotopes in the fractions were similar.  相似文献   

17.
In acid soils in the Eastern Plains of Colombia, forage grasses planted on land prepared before the previous dry season produced 40–50% more dry matter than when land was prepared immediately before planting. Virtually no NO3 accumulated in surface (0–10 cm) soil from three native undisturbed savanna sites. Where land was ploughed before the dry season, NO3 levels increased gradually after a 2–3 month lag, and dropped at the beginning of the rains. In samples incubated for 4 weeks, more NO3 accumulated in the wet than the dry season. A similar 2–3-month lag occurred when land was ploughed after the dry season. NH4+ levels were higher in ploughed than savanna soils, and rose in all soils at the beginning of the rains. More NO3 and NH4+ accumulated on incubation in pots than in soil cores. Forage grasses inhibited NO3 accumulation in the soil, relative to plant-free plots, and legumes stimulated it. N fertilization overcame this inhibition except in the case of Brachiaria humidicola .  相似文献   

18.
Abstract. Slurry from farm animals may contaminate water supplies, rivers and bathing waters with faecal coliforms, such as Escherichia coli . Where animals harbour the O157 strain the hazard to human health is particularly high, but both the hazard level, and the low incidence and sporadic nature of the excretion of E. coli O157 make it difficult to study this strain under field conditions. The survival of total E. coli and of E. coli O157 were compared in the laboratory for two soils under controlled temperature and moisture. E. coli O157 die-off rate was the same as or quicker than for total E. coli . This result meant that field experiments studying the fate of total E. coli should give a satisfactory evaluation of the risk of water contamination by the O157 strain. In four field experiments at three sites, slurry containing total E. coli numbers of 2.2 × 104 to 5.7 × 105 colony forming units per mL (c.f.u. mL–1) was applied to drained field plots. Field die-off was faster than expected from laboratory experiments, especially in one experiment where two weeks dry weather followed application. In all but this experiment, the first drain flow events after slurry application led to very high E. coli concentrations in the drains (103 to 104 c.f.u. mL–1). E. coli O157 was present in the slurry used for two of the experiments (33 c.f.u. per 100 mL in each case). However the proportion of E.coli O157 was very low (about 1 in 105) and it was not detected in the drainage water. After the first week E. coli drainage water numbers decreased rapidly but they were 1–10 c.f.u. mL–1 for much of the sampling period after slurry application (1–3 months).  相似文献   

19.
The systematic nature of the 34S natural abundance variations (δ34S) in a prairie and boreal forest ecosystem enabled construction of hypotheses regarding the origin and cycling of S in the two soil-plant systems. By considering the 34S abundance variations in relation to soil S transformations, a better understanding of S isotope fractionation in soils was also achieved.
The δ34S values suggest that atmospheric S becomes increasingly more important as a S input as pedogenesis proceeds in these soils. The origin and movement of sulphate salt in a saline seep was evaluated using δ34S values, demonstrating the usefulness of the δ34S technique in soil salinity studies. The 34S-enrichments and depletions found in soil organic S fractions were consistent with postulated differences in lability, mobility, and turnover rate. Wheat plants growing on the saline, sulphate-saturated prairie soil were found to be enriched in 34S relative to surrounding S sources, providing indirect evidence for release of 34S-depleted H2S by the plants as a S-stress relief mechanism.  相似文献   

20.
Abstract. When over exploited and coupled with climatic conditions, tropical soils are subject to increased erosion and a loss of soil organic matter. Countermeasures include the incorporation of organic materials such as crop and animal residues. We studied the effect of adding crop residues and manure to soil, at five sites in Ethiopia, on carbohydrate properties, aggregate stability and the C and N distribution within water-stable aggregates. The effects of organic amendments varied between sites. The largest content of carbohydrates was obtained in the control treatment at Holeta, Ginchi (90 kg ha–1 mustard meal), Jimma (5 t ha–1 cow dung + 9 t ha–1 coffee husk), Awassa (forested soil), and Sirinka (soil alley-cropped with Leuceanae ). The aggregate stability of these soils was highly correlated with the OM content but not with carbohydrates. The smaller aggregates (<1.00 mm) accumulated more carbohydrates than the larger (>1.00 mm), thereby suggesting a protecting effect within the finer soil fractions. A protecting role played by humified OM components was also indicated by the C and N distribution as well as the C:N ratios which showed preferential accumulation in small rather than in large aggregates. The isotopic 13C-OC values of carbohydrate extracts were generally low, suggesting that OM was from plants with C3 photosynthetic pathways. Soil treatments with maize alone or combined with coffee husks at Jimma decreased the δ13C‰ values slightly, revealing that maize contributed a share of the labile OM. Despite the improvement in the soil OM content, neither the carbohydrate content nor the aggregate stability were increased to the level of the forested sites, suggesting that the additions of crop residues and manure were not alone sufficient to restore the soil physical quality.  相似文献   

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