共查询到19条相似文献，搜索用时 250 毫秒
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《中国奶牛》2020,(7)
为了确诊新疆某规模化奶牛场犊牛腹泻疾病病原,剖检4头腹泻犊牛采集其脏器以及血清样品,再采集另外6头腹泻犊牛血清样品,对脏器样品进行细菌的分离鉴定,采用ELISA的方法对血清样品进行病毒性腹泻-黏膜病抗原检测,对轮状病毒、冠状病毒和细小病毒进行抗体检测,对抗体阳性样品采用PCR方法进一步进行确诊。结果发现,4头份脏器样品均检测出沙门氏菌、大肠杆菌,10份血清样品中均未检测出病毒性腹泻-黏膜病病毒抗原、轮状病毒和冠状病毒抗体,8份血清样品中检测出细小病毒抗体,7份血清样品中检测出细小病毒DNA。结果表明,该牛场犊牛腹泻主要由沙门氏菌、大肠杆菌和细小病毒混合感染引起。本试验结果可为该场犊牛腹泻诊治提供参考。 相似文献
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为了调查某规模化奶牛场犊牛腹泻的发病原因,试验采用血清抗体检测、细菌分离培养、寄生虫卵镜检和病毒核酸检测方法对腹泻犊牛的血样和粪便进行了检查。结果表明,该奶牛场牛病毒性腹泻/黏膜病病毒抗体阳性率为65.00%,牛轮状病毒抗体阳性率25.00%,牛冠状病毒抗体阳性率15.00%;分离到1 株结肠弯曲杆菌和1 株空肠弯曲杆菌;所检腹泻犊牛粪便和血液样品中,4 份粪便样品和1 份血液样品检出牛冠状病毒,2 份粪便样品检出轮状病毒,1 份粪便样品和1 份血液样品检出牛病毒性腹泻/黏膜病病毒,同一份粪便样品中同时检出牛病毒性腹泻/黏膜病病毒和牛冠状病毒;通过镜检,球虫卵囊检出率77.78%,在1 份粪样中发现蛔虫卵,2 份粪样中发现其他线虫卵。该牛场流行性腹泻的原因可能为夏季高温潮湿引起犊牛免疫力下降导致的多病原体感染。 相似文献
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《黑龙江畜牧兽医》2019,(24)
为了解巴州地区犊牛腹泻的流行情况,分别抽检114头腹泻犊牛(本地杂交黄牛、黑白花奶牛、新疆牦牛)的脏器、血清和粪便样品共342份,对脏器进行细菌的分离培养鉴定,采用ELISA方法对血清进行副结核抗体检测,对粪便进行轮状病毒、冠状病毒、病毒性腹泻/黏膜病病毒的病原检测和寄生虫的分离鉴定。结果表明:沙门氏杆菌阳性率为42.1%,大肠杆菌阳性率为38.6%,病毒性腹泻病毒阳性率为24.6%,轮状病毒阳性率为21.9%,冠状病毒阳性率为14.0%,球虫阳性率为13.2%,隐孢子虫阳性率为14.0%。肉牛、牦牛细菌阳性率显著高于病毒和寄生虫(P0.05),奶牛病毒性腹泻病毒和轮状病毒阳性率显著高于细菌和寄生虫阳性率(P0.05)。说明巴州地区犊牛腹泻主要由沙门氏杆菌、大肠杆菌引起,不同犊牛品种感染的病原有差异。 相似文献
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牛病毒性腹泻病毒、大肠杆菌和奇异变形杆菌混合感染致犊牛腹泻的研究 总被引：2，自引：1，他引：1
为确定甘肃省临夏州某奶牛场犊牛腹泻的病因,并提供合适的治疗方案和防控措施,试验采集该牛场13头腹泻犊牛的粪便和血清,通过胶体金技术、ELISA方法、细菌分离鉴定、Kirby-Bauer法分别进行病毒病原学检测、病毒血清学抗体检测、病原菌鉴定和药物敏感性试验。病毒学检测结果显示,13份粪样中未检测出牛轮状病毒（BRV）、牛冠状病毒（BCV）的抗原,牛病毒性腹泻病毒（BVDV）抗原阳性率为23.08%（3/13）;未检出BRV和BCV的抗体,BVDV血清学抗体阳性率为38.46%（5/13）。病原菌检测结果显示,13份粪便样品中,分离出13株大肠杆菌和7株奇异变形杆菌。药敏试验表明,分离的大肠杆菌和奇异变形杆菌对20种常规药物均产生了不同程度的耐药,且无对两种细菌均有效的药物。此次犊牛腹泻是由BVDV、大肠杆菌、奇异变形杆菌混合感染引起的,且大肠杆菌和奇异变形杆菌的耐药现象严重,本试验结果为该牛场进一步治疗此次的犊牛腹泻病提供了合理有效的依据。 相似文献
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我国14省市呼吸道综合征患牛冠状病毒感染的检测 总被引：2，自引：0，他引：2
牛冠状病毒(bovine coronavirus, BCoV)是导致牛消化道和呼吸道疫病的主要病原之一,其在国内养殖业的感染报道较少。本试验对牛冠状病毒感染情况及其与临床罹患呼吸道疾病综合征犊牛的临床症状相关性开展研究。在奶牛养殖量较大的14个省市随机选择29个规模化牧场,对未断奶的犊牛进行呼吸道疾病综合征评估,选取单一症状典型的犊牛采集血清和鼻咽拭子。在176份样品中BCoV阳性共38份,阳性率为21.59%。统计发现,采自黑龙江的病料中冠状病毒检出率最高(70%),新疆次之,山西、上海、安徽未检出BCoV阳性样品;阳性病例与呼吸道疾病疾病综合征临床症状的相关性分析表明,病畜的发热、鼻腔分泌物性状改变与感染牛冠状病毒密切相关。本研究为牛冠状病毒感染导致犊牛呼吸道疾病综合征的临床诊断提供了参考。 相似文献
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牛病毒性腹泻病毒、大肠杆菌和奇异变形杆菌混合感染致犊牛腹泻的研究 总被引：2，自引：2，他引：0
为确定甘肃省临夏州某奶牛场犊牛腹泻的病因,并提供合适的治疗方案和防控措施,试验采集该牛场13头腹泻犊牛的粪便和血清,通过胶体金技术、ELISA方法、细菌分离鉴定、Kirby-Bauer法分别进行病毒病原学检测、病毒血清学抗体检测、病原菌鉴定和药物敏感性试验。病毒学检测结果显示,13份粪样中未检测出牛轮状病毒(BRV)、牛冠状病毒(BCV)的抗原,牛病毒性腹泻病毒(BVDV)抗原阳性率为23.08%(3/13);未检出BRV和BCV的抗体,BVDV血清学抗体阳性率为38.46%(5/13)。病原菌检测结果显示,13份粪便样品中,分离出13株大肠杆菌和7株奇异变形杆菌。药敏试验表明,分离的大肠杆菌和奇异变形杆菌对20种常规药物均产生了不同程度的耐药,且无对两种细菌均有效的药物。此次犊牛腹泻是由BVDV、大肠杆菌、奇异变形杆菌混合感染引起的,且大肠杆菌和奇异变形杆菌的耐药现象严重,本试验结果为该牛场进一步治疗此次的犊牛腹泻病提供了合理有效的依据。 相似文献
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宁夏地区犊牛腹泻的病原调查 总被引：2，自引：0，他引：2
为了解宁夏地区奶牛犊牛腹泻的病原微生物种类及流行病学情况,2017年6月至12月对宁夏地区20个牧场有临床腹泻症状的犊牛,随机采集127份腹泻粪便样品,采用快速检测试剂盒对轮状病毒、隐孢子虫、大肠杆菌和贾第鞭毛虫4种病原微生物进行了抗原检测。结果显示:在127份腹泻粪样中,轮状病毒的阳性检出率为32. 28%(41/127),隐孢子虫的阳性检出率为25. 20%(32/127),大肠杆菌阳性检出率为11. 02%(14/127),贾第鞭毛虫阳性检出率为4. 72%(6/127)。结果表明:宁夏部分奶牛养殖区犊牛已经存在着较为严重的轮状病毒和隐孢子虫病感染,此调查为宁夏制定科学有效的综合防控措施提供了有效依据,对促进宁夏地区奶产业健康持续发展具有重要意义。 相似文献
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《中兽医医药杂志》2016,(2)
为了解我国主要奶业产区导致犊牛腹泻的主要病原,于2015年6月至2016年1月,采集华北、西北、东北主要奶业产区内的176份犊牛腹泻粪样,并对样品中的牛冠状病毒(BCOV)、牛轮状病毒(BRV)、牛病毒性腹泻病毒(BVDV)、大肠杆菌K99、隐孢子球虫、肠兰伯氏鞭毛虫和其他致病菌进行检测,同时结合临床收集的资料,分析病原与临床症状的相关性。结果表明,不同奶业产区内导致犊牛腹泻的病原存在一定差异,西北地区导致犊牛腹泻的病原主要是痢疾杆菌,而华北地区则是隐孢子球虫和肠兰伯氏鞭毛虫,东北地区主要是牛轮状病毒和肠兰伯氏鞭毛虫,且病原感染与犊牛腹泻的临床症状存在明显相关性。 相似文献
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Zhu W Dong J Haga T Goto Y Sueyoshi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):531-534
Bovine coronavirus (BCoV) and group A bovine rotavirus (BRV) are two of major causes for neonatal calf diarrhea. In the present study, a one-step duplex RT-PCR was established to detect and differentiate BCoV and group A BRV from fecal samples. The sensitivity of this method for BCoV and group A BRV was 10 PFU/100 μl and 1 PFU/100 μl, respectively. Twenty-eight diarrhea fecal samples were detected with this method, the result showed that 2 samples were identified as co-infected with BCoV and group A BRV, 26 samples were group A BRV positive, and 2 samples were negative. It proved that this method is sensitive for clinical fecal samples and is worth applying to laboratory diagnosis for BCoV and group A BRV. 相似文献
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G Marsolais R Assaf C Montpetit P Marois 《Canadian journal of veterinary research》1978,42(2):168-171
During this study, 134 samples have been examined for the detection of the viruses associated with neonatal calf diarrhea. The presence of Nebraska viruses (rotavirus and coronavirus) has been demonstrated by using the electron microscope and the fluorescent antibody techniques while the presence of other viruses has been detected by the observation of a cytopathic effect on monolayer cells of calf testis. The Nebraska viruses have been demonstrated in 107 (80%) out of 134 field case specimens. An association of rotaviruses and coronaviruses was found in 58 cases (54%) whilst the coronaviruses and the rotavirus were found singly in 34 cases (53%) and in 15 cases (14%) respectively. Four bovine virus diarrhea viruses, two infectious bovine rhinotracheitis viruses and two enteroviruses have also been isolated in the preceding 107 Nebraska positive specimens. For the detection of the Nebraska viruses, the fluorescent antibody techniques were more sensitive than the electron microscopy. However, those two techniques must be used simultaneously for a better detection of a greatest possible number of cases. 相似文献
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Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces 总被引：2，自引：0，他引：2
Enzyme-linked immunosorbent assay (ELISA) was compared with electron microscopy in the examination of faeces from experimental calves and showed 100 per cent agreement in the detection of 19 bovine coronavirus and 15 bovine rotavirus electron microscope positive samples. In a limited field survey of calf diarrhoea 75 selected faeces were examined independently by ELISA and electron microscopy and the agreement between the two tests was 95 per cent for coronavirus and 84 per cent for rotavirus. A further comparison was made with 74 samples submitted for routine diagnosis and this yielded agreements of 82 per cent (coronavirus) and 89 per cent (rotavirus). Factors contributing to discrepant results were examined and the relative advantages and disadvantages of the two tests for routine detection of these enteric viruses are discussed. 相似文献
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为了解青海省部分牛群中牛病毒性腹泻病毒（bovine viral diarrhea virus,BVDV）、牛轮状病毒（bovine rotavirus,BRV）和牛冠状病毒（bovine coronavirus,BCV）3种牛病毒性腹泻病原的感染现状,本研究采用RT-PCR方法首次对2012～2013年青海省部分地区的32份具有腹泻症状的临床病料及152份健康牛粪便样品进行了BVDV、BRV、BCV的核酸检测与分析。结果显示,32份腹泻牛病料样品中BVDV、BRV、BCV的阳性率分别为65.63%（21/32）、18.75%（6/32）、34.38%（11/32）,且存在2种或3种病原的混合感染;152份健康牛粪便样品中BVDV、BRV、BCV的阳性率分别为3.95%（6/152）、1.97%（3/152）、0（0/152）。该结果表明青海省部分牛群中普遍存在BVDV、BRV、BCV的感染,且混合感染现象严重,需进一步加强青海省地区牛病毒性腹泻病原的综合防控。 相似文献
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Calf diarrhea is a kind of digestive tract disease caused by a variety of pathogenies, which has a great impact on the survival, growth and development of calves. Escherichia coli, bovine rotavirus, bovine coronavirus and other pathogens often cause diarrhea in calves, in addition, the secondary infection and mixed infection also occurre in different pathogens, which lead to great difficulty for prevention of calf diarrhea. A variety of vaccines have been developed for the prevention and treatment of calf diarrhea at home and abroad. Now, except only minor commercial calf diarrhea vaccines have been found in abroad, there is no one multivalent commercial calf diarrhea vaccine in China. Based on previous study, the progress of pathogens and vaccines for calf diarrhea were elaborated in the study, which could provide exploitation references for the commercial calf diarrhea vaccine in China. 相似文献
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ZHANG Shu-dong LI Hao WAN Qiang XU Fa-rong JUMABIEKE Xialabayi PAN Bao-liang 《中国畜牧兽医》2017,44(8):2424-2430
This study was aimed to explore the pathogenesis of calf diarrhea in a large scale dairy farm in Shanxi province and the correspond comprehensive prevention and control measures. By investigating the incidence of calf diarrhea in the field, rotavirus, coronavirus,E. coli F5 (K99),Cryptosporidium parvum, coccidia and Salmonella were detected through experimental diagnosis, and the suspicious pathogenic bacteria was isolated and cultivated,which was identified with morphology and serology, simultaneously sensitive antibiotics were screened according to the antibiotic susceptibility tests, which contained 18 kinds of common antibiotics. The results showed that the clinical diagnosis and laboratory tests basically ruled out the possibility of E. coli F5 (K99), rotavirus, coronavirus, Cryptosporidium parvumand coccidia infection, and the isolated suspected bacteria had Salmonella colony characteristics and bacterial morphology, which showed positive reaction to Salmonella O polyvalent A-F serum, so we had identified that it was Salmonella diarrhea.The antibiotic susceptibility tests results showed that Salmonella was highly sensitive to ceftriaxone, gentamicin, cephradine and amikacin, which could be recommended for clinical treatment. Above all, this study proposed the specific prevention and treatment of the disease, and better control effect was achieved. This study initially provided a reference for the diagnosis and treatment of Salmonella of calf diarrhea, with great clinical significance in the early diagnosis and treatment of pathogens of calf diarrhea. 相似文献
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腹泻是犊牛最常见的临床问题之一,严重影响犊牛健康和养殖业的发展。本文介绍了加强产房接产管理、初乳质量检测、初乳消毒与饲喂的标准化管理以及免疫球蛋白转移对预防犊牛腹泻的详细内容;重点介绍了犊牛三种营养缺乏引起腹泻的临床症状、病理变化与防控要点;全面介绍了轮状病毒性腹泻、大肠杆菌病腹泻、沙门菌病腹泻、小球隐孢子虫病腹泻和球虫病性腹泻的症状、诊断与防控要点;为规模化牧场兽医全面掌握犊牛腹泻的防控提供理论依据与技术支持。 相似文献