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1.
为探究参虎败毒颗粒抗猪繁殖与呼吸综合征病毒（PRRSV）的作用,将20头PRRSV阴性仔猪随机平均分为4组,即提前药物处理感染组（A组）、感染同时药物处理组（B组）、无药物处理感染组（C组）和无药物处理不感染组（D组）。A和B组分别于攻毒前3 d和攻毒时在基础日粮中添加参虎败毒颗粒（每头10 g·d-1）,连续饲喂至攻毒后28 d,每日测量体温,在攻毒后第14天,每组随机处死仔猪1头,观察病理变化,RT-qPCR检测肺组织病毒载量,并在攻毒后第1、3、5、7、14、21、28、35天采全血和血清样品进行PRRSV核酸和抗体检测。结果发现,A和B组仔猪体温在攻毒后第6天上升至40℃以上,第12天时恢复正常;C组仔猪体温在第5天上升至40℃以上,持续至试验结束仍未恢复。A、B组仔猪肺组织病变较轻,肺泡结构轻度损伤,支气管内有少量炎性渗出物,周围有少量炎性细胞浸润;C组仔猪肺组织病变严重,肺泡结构破坏严重,支气管内有大量炎性渗出物和炎性细胞浸润。A组在攻毒后3、5、7和14 d病毒载量极显著低于C组（P<0.01）;B组在攻毒后3和5 d病毒载量极显著低于C组（P<0.01）,第7和14天病毒载量显著低于C组（P<0.05）。A、B组PRRSV抗体在攻毒后第21天达到峰值,C组在28 d达到峰值。以上结果可知,参虎败毒颗粒能够显著减轻PRRSV造成的仔猪肺部病理损伤,降低病毒血症;添加药物可使PRRSV特异性抗体峰值提前,减少病毒血症持续时间,证明参虎败毒颗粒可用于预防或治疗PRRSV感染,降低PRRSV对仔猪的影响。  相似文献   

2.
为了解香菇多糖和七清败毒颗粒联合应用对集团化猪场猪繁殖与呼吸障碍综合征(PRRS)的防控效果,本研究选取3~4胎次、体重为(268.00±33.59)kg的母猪100头,随机分为2组(每组5个重复,每个重复10头猪),分别为对照组和试验组。对照组饲喂基础饲粮,试验组在基础饲粮中添加2.5 g/(头·d)香菇多糖+5.0 g/(头·d)七清败毒颗粒。试验对母猪繁殖性能、血清生化指标、PRRSV抗体水平、仔猪脐带血中PRRSV载量进行检测。试验期90 d。结果表明:在母猪繁殖性能方面,与对照组相比,试验组窝均产仔数、平均日增重极显著提高(P<0.01),窝均死胎数极显著降低(P<0.01)。在血清生化指标方面,第30天,试验组血清谷草转氨酶(AST)活性显著低于对照组(P<0.05);第90天,试验组血清谷丙转氨酶(ALT)、AST活性及总胆红素(TBIL)含量显著低于对照组(P<0.05),血清尿素(UN)含量极显著降低于对照组(P<0.01)。在PRRSV抗体检测方面,第30天,试验组S/P值极显著低于对照组(P<0.01);第90天,试验组显著低于对照组(P<0.05),离散度有降低趋势,符合正态分布。在仔猪脐带血PRRSV抗原检测方面,对照组抗原阳性率为10%,试验组为0。综上所述,感染PRRS的集团化猪场应用香菇多糖和七清败毒颗粒可以有效地提升母猪繁殖性能、影响部分血清生化指标水平、维持猪群PRRSV抗体S/P值稳定、降低仔猪PRRSV阳性率,起到很好地防控PRRS的效果。  相似文献   

3.
为了建立猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)游离受体定量检测方法,本试验用表达猪唾液酸黏附素(Sn)游离受体的重组腺病毒rAd-Sn4D-Fc感染PK-15细胞,以细胞培养上清纯化的Sn4D-Fc游离受体作为标准抗原,鼠抗猪Sn免疫血清为一抗,生物素标记兔抗猪Sn多克隆抗体为二抗,建立定量检测Sn4D-Fc游离受体的双抗体夹心ELISA方法;用rAd-Sn4D-Fc注射仔猪,定期采集血清进行游离受体检测。结果显示,纯化Sn4D-Fc标准抗原的纯度为94.3%,能被Sn免疫血清识别;一抗的最佳工作浓度为5μg/mL蛋白,二抗的最佳稀释度为1∶4 000,夹心ELISA检测标准抗原的灵敏度为0.4ng/mL,对照抗原无交叉反应;夹心ELISA能从重组腺病毒注射猪血清中检测到Sn4D-Fc游离受体,最高表达量为6.54ng/mL,持续时间为15d。研究结果表明,建立的双抗体夹心ELISA可用于PRRSV游离受体的体内外定量检测。  相似文献   

4.
将猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)单感染和共感染6周龄健康仔猪,采用实时定量PCR(Real-time PCR)对血清、组织(心、肝、肺、肾、胰、脾、胸腺、扁桃体、腹股沟淋巴结、肠系膜淋巴结)中的PRRSV和PCV2载量进行检测,分别采用间接ELISA和间接IFA对血清中的PRRSV和PCV2特异性抗体进行检测,采用MTT法对猪外周血淋巴细胞(PBLC)的增殖能力进行检测.结果,PRRSV/PCV2共感染组血清、组织中PRRSV的载量均显著高于PRRSV单感染组,PCV2的载量均显著高于PCV2单感染组;共感染组PRRSV和PCV2特异性抗体阳转的时间均晚于并且效价均显著低于PRRSV或PCV2单感染组;共感染组和单感染组PBLC的增殖能力均受到抑制,其严重程度依次为:PRRSV/PCV2组>PRRSV组>PCV2组.由此表明,PRRSV和PCV2在猪体内对彼此的增殖具有促进作用;PRRSV和PCV2共感染对猪的免疫抑制具有加重作用.  相似文献   

5.
为了建立猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus,PRRSV）游离受体定量检测方法,本试验用表达猪唾液酸黏附素（Sn）游离受体的重组腺病毒rAd-Sn4D-Fc感染PK-15细胞,以细胞培养上清纯化的Sn4D-Fc游离受体作为标准抗原,鼠抗猪Sn免疫血清为一抗,生物素标记兔抗猪Sn多克隆抗体为二抗,建立定量检测Sn4D-Fc游离受体的双抗体夹心ELISA方法;用rAd-Sn4D-Fc注射仔猪,定期采集血清进行游离受体检测。结果显示,纯化Sn4D-Fc标准抗原的纯度为94.3%,能被Sn免疫血清识别;一抗的最佳工作浓度为5 μg/mL蛋白,二抗的最佳稀释度为1：4 000,夹心ELISA检测标准抗原的灵敏度为0.4 ng/mL,对照抗原无交叉反应;夹心ELISA能从重组腺病毒注射猪血清中检测到Sn4D-Fc游离受体,最高表达量为6.54 ng/mL,持续时间为15 d。研究结果表明,建立的双抗体夹心ELISA可用于PRRSV游离受体的体内外定量检测。  相似文献   

6.
文章旨在研究复合益生菌剂对仔猪生长性能、血清免疫指标及粪便微生物的影响,试验选取60头30日龄体重为(7.74±0.38)kg的健康断奶仔猪,随机分为2组,每组3个重复,每个重复10头仔猪。试验组按照1.0×109 CFU/头添加复合益生菌剂,每天饮水饲喂,试验共28 d。结果显示,试验组日均增重、日均采食量分别极显著(P<0.01)、显著(P<0.05)高于对照组;试验组血清IgG含量极显著高于对照组(P<0.01),血清IgM、IL-2、IL-1β和TNF-α含量均显著高于对照组(P<0.05);试验组第14天粪样中乳酸杆菌数显著高于对照组(P<0.05),第28天极显著高于对照组(P<0.01),第28天样中大肠杆菌数极显著低于对照组(P<0.01)。试验组第14天粪样中乳酸杆菌数显著高于第0天(P<0.05),第28天粪样中乳酸杆菌数显著高于第14天(P<0.05),第28天粪样中乳酸杆菌数极显著高于第0天(P<0.01),第28天粪样中大肠杆菌数极显著低于第0天和第14天的粪样(P<0.0...  相似文献   

7.
为了建立猪繁殖与呼吸综合征病毒(PRRSV)接触感染模型,将易感仔猪与PRRSV人工感染仔猪在隔离器中同居饲养,每日观察记录体温变化和临床症状,定期采集血清和粪便样品,用荧光定量RT-PCR检测病毒血症和粪便排毒水平;对病死猪和试验结束迫杀猪进行剖检和肺脏组织病理学检查,并检测各器官病毒载量和组织分布。结果显示:接触感染仔猪出现高热和PRRS症状时间分别较人工接种猪推迟2~3d;第3天起血清检测PRRSV阳性,第10天病毒血症上升至较高水平(108.6拷贝/mL);第5天起粪便检测PRRSV阳性,第7天粪便排毒量上升至较高水平(103.9拷贝/0.1g);3头接触感染仔猪分别于同居后第12天和第13天死亡,较人工接种猪推迟1~2d,病死猪的主要器官病毒载量以及剖检和肺脏显微病理变化与人工接种病死猪相似。这些结果表明,本研究建立的接触感染仔猪模型可用于PRRS疫苗免疫效果评价。  相似文献   

8.
为研究不同毒力的PRRSV对仔猪肺脏和外周免疫器官损伤的差异,本实验分别采用PRRSV变异株(HuN4株)和PRRSV经典株(CH-1a株)感染35日龄健康的断奶仔猪,并在感染后0 d、3 d、7 d、10 d和14 d各迫杀3头,检测肺、颌下淋巴结、肠系淋巴结、腹股沟淋巴结、扁桃体和脾脏的病毒载量及病理变化情况,同时检测血清中抗PRRSV的抗体水平。结果表明:感染后3 d肺脏及各免疫器官可检测到病毒,HuN4感染组病毒载量比CH-1a感染组病毒载量高1 000倍;HuN4感染组病毒载量峰值出现在感染后10 d,而CH-1a感染组维持着较低水平的病毒载量。组织病理学检测显示HuN4感染组淋巴结内淋巴细胞显著减少,呈空泡状;CH-1a感染组淋巴结内淋巴细胞轻度减少,呈星隙状。本实验表明HuN4株比CH-1a株对肺和外周免疫器官造成更严重的损伤。  相似文献   

9.
将猪生殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV2）单感染和共感染的6周龄仔猪，于感染前和感染后第3、7和10d，采用血常规法、SWC3a单色流式细胞术以及CD3／CD4／CD8三色流式细胞术分析了感染猪外周血中白细胞、粒细胞、单核细胞、NK细胞、弦T细胞的含量。结果，感染后第3d和第7d，PRRSV感染组、PCV2感染组以及PRRSV＋PCV2共感染组猪的白细胞、单核细胞、粒细胞、NK细胞、弦T细胞总数显著下降，并且共感染组比单感染组下降更加严重。结果表明，PRRSV＋PCV2共感染对仔猪外周血天然免疫细胞的影响具有协同效应，意味著在感染旱期可导致更加严重的先天免疫抑制。  相似文献   

10.
为了解猪繁殖与呼吸综合征病毒(PRRSV)VR2332弱毒株经滴鼻和注射两种途径接种仔猪的病毒血症、抗PRRSV抗体以及抗PRRSV中和抗体的差异,选取20日龄健康仔猪9头,随机分3组,即滴鼻组、注射组和对照组。在感染后第0、3、7、11、15、19、23、27、31、35、39、43天前腔静脉采血,于第43天采血后摘取猪的脾、颌下淋巴结、腹股沟淋巴结和肠系膜淋巴结,并无菌收集猪肺泡巨噬细胞(PAM),通过实时荧光定量PCR方法检测病毒载量。用ELISA方法检测抗PRRSV抗体效价,用基于PAM的中和试验检测抗PRRSV中和抗体效价。结果显示:仔猪感染PRRSV后,①滴鼻组第3天只在2头猪血清中检测到PRRSV,第15-19天病毒血症达到高峰,病毒拷贝数均大于104拷贝·μL-1,第27天后病毒血症消失;注射组第3天从3头仔猪血清中均检测到病毒,在第15-23天病毒血症达到高峰,其中第19天病毒拷贝数大于105拷贝·μL-1,病毒血症从第31天后开始消失。②从几种淋巴结和PAM中均检测到PRRSV,且注射组的PRRSV拷贝数稍高于滴鼻组。③均在第27天从血清中检测到抗PRRSV抗体,随后抗体水平一直处于逐渐上升趋势,且第35-43天,注射组的抗PRRSV抗体水平高于滴鼻组。④滴鼻组在第35天之前抗PRRSV中和抗体效价都小于1∶2,第43天抗PRRSV中和抗体效价为1∶2;注射组在第3、11和19天的抗PRRSV中和抗体效价均小于1∶2,第27、35、43天的中和抗体效价分别为1∶4、1∶8、1∶4。试验结果表明,注射组的病毒拷贝数一直为滴鼻组的10～100倍,且病毒血症持续时间更长;注射组血清中抗PRRSV抗体产生的时间比滴鼻组早,并且抗体水平比滴鼻组高;滴鼻组抗PRRSV中和抗体水平很低,注射组产生的抗PRRSV中和抗体比滴鼻组早且抗体水平高。综上所述,VR2332弱毒株经注射免疫比滴鼻免疫能刺激猪产生更高水平的中和抗体,但同时也会引发更高水平的病毒血症。  相似文献   

11.
An epidemiological study of porcine reproductive and respiratory syndrome (PRRS) within pig herds was conducted in 8 intensive farrow-to-finish pig farms. Persistence of PRRS virus (PRRSV) in pig herds was demonstrated by regular postmortem examination on 2 farms for a period of 2 y. Virus isolation and serum neutralization (SN) tests were performed on the sera collected from 9 groups of pigs (10 pigs/group) of various ages on 8 pig farms. Except for 1 farm, isolation rates of PRRSV reached the highest level of 70 to 100% of pigs 6 to 8 wk of age, which coincided with the lowest levels of maternal immunity. In 1 pig herd, sows (39 in total) with SN titers of < or = 1:2, 1:4-1:8, and > or = 1:16 were designated as groups 1, 2, and 3, respectively. Sera were obtained from their progeny (3 pigs randomly selected from each litter) at various ages from 0 to 22 weeks. A positive correlation (r = 0.377, P < 0.001) between the SN titers of sows and those of their progeny (1-week-old piglets) was observed. Pigs at the age of 6 wk, only 7.9% of group 1 pigs compared to 72.4% of group 3 pigs were seropositive. A significant difference (P < 0.01) in the percentage of pigs with PRRSV viremia among the 3 groups was observed, with the lowest level found in group 3 pigs. The isolation rates of PRRSV from serum reached the maximum at the age of 9 wk for all 3 groups. The results indicated that passively acquired serum antibodies conferred a protective effect for piglets; however, loss of passive immunity at various ages of pigs produced susceptible pigs that resulted in PRRSV persistence in the pig herds. Pigs 6 to 9 weeks old were the major reservoir for PRRSV in farrow-to-finish pig herds.  相似文献   

12.
对感染猪繁殖和呼吸综合征病毒（PRRSV）对猪扁桃体病理学变化进行了试验研究。结果发现攻毒猪的4种扁桃体均有一定程度的组织病理学变化,具体表现为扁桃体上皮细胞坏死、脱落,结缔组织和上皮内淋巴细胞浸润,实质中部分淋巴细胞变性、坏死、破碎,淋巴小结不明显,淋巴窦内嗜酸性粒细胞浸润。试验表明PRRSV可以诱导扁桃体产生免疫应答,而过度的PRILSV感染除引起炎性细胞聚集外,还可造成淋巴结坏死性炎症的特征性变化。这一结果将为理解PRRSV同机体的免疫结构相互作用的机理和免疫防制等更深层次的研究奠定一定的理论基础。  相似文献   

13.
The objective of this study was to compare the safety of all modified live virus vaccines commercially available in Europe against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) under the same experimental conditions. For this purpose, one hundred and twenty three-week-old piglets, divided into five groups, were used. On day 0 of the experiment, nine pigs per group were removed and the remaining fifteen were vaccinated with the commercial vaccines Ingelvac PRRS MLV, Amervac PRRS, Pyrsvac-183 and Porcilis PRRS by the IM route or were mock vaccinated and used as controls. On day 3, the nine unvaccinated pigs were re-introduced into their respective groups and served as sentinel pigs. Clinical signs were recorded daily and lung lesions were determined on days 7, 14 and 21, when 5 vaccinated pigs per group were euthanized. Blood samples and swabs were taken every three days and different organs were collected at necropsy to determine the presence of PRRSV. None of the vaccines studied caused detectable clinical signs in vaccinated pigs although lung lesions were found. Altogether, these results indicate that all vaccines can be considered clinically safe. However, some differences were found in virological parameters. Thus, neither Pyrsvac-183 nor Porcilis PRRS could be detected in porcine alveolar macrophage (PAM) cultures or in lung sections used to determine PRRSV by immunohistochemistry, indicating that these viruses might have lost their ability to replicate in PAM. This inability to replicate in PAM might be related to the lower transmission rate and the delay in the onset of viremia observed in these groups  相似文献   

14.
Porcine reproductive and respiratory syndrome virus (PRRSV) is endemic in most parts of Asia, where genotype I and II strains of diverse virulence may coexist. This study evaluated the outcome of infection with a highly virulent Asian genotype II PRRSV isolate in piglets vaccinated with a genotype I vaccine. Twenty-one 3-week-old piglets were divided in three groups: Pigs in group V (n=8) were vaccinated with an attenuated genotype I commercial PRRSV vaccine, while pigs in group U (n=8) and a control group (group C; n=5) were unvaccinated; 6 weeks later, pigs in groups V and U were challenged intranasally with a highly virulent strain of genotype II PRRSV (1×10(5) 50% tissue culture infectious doses/mL), while pigs in group C received a placebo. Over a period of 21 days after challenge, vaccinated pigs had significantly lower mortality (0/8 versus 2/8), fewer days of fever, a lower frequency of catarrhal bronchopneumonia, higher weight gains (13.4 versus 6.6 kg) and lower levels of viraemia compared to unvaccinated challenged pigs. Immunisation with a genotype I attenuated PRRSV vaccine provided partial protection against challenge with a highly virulent genotype II strain.  相似文献   

15.
谭涛 《猪业科学》2021,38(8):51-59
猪繁殖与呼吸综合征（PRRS）俗称猪蓝耳病,是由PRRS病毒（PRRSV）引起的传染病,分为欧洲型和北美型,是目前养猪业最重要的猪病之一。PRRS每年给美国养猪业造成的损失为6.64亿美元（约40亿人民币）,中国的养猪量数倍于美国,因此该病在中国造成的损失应该远大于美国。该病对母猪群的影响主要是降低分娩率,降低产仔数和窝均断奶仔猪数。我国的PRRS以北美型为主,感染后会引起母猪群的繁殖障碍,仔猪、生长育肥猪群的呼吸道问题。2006年我国暴发了由变异株PRRSV引起的高致病性PRRS,该病在全国范围内流行,重创了中国养猪业[1]。2013年以后,类NADC-30毒株开始在国内逐渐扩散传播,各种重组毒株也不断出现,PRRS的防控变得更加复杂[2-3]。PRRSV容易变异、毒株多样、感染时间长、防控难度较大。猪群感染PRRS后会造成免疫抑制,易出现各种混合感染和继发感染,这增加防控的难度。文章回顾了PRRS的临床症状、病理变化、病原特性、传播途径、诊断方法与防控措施。在PRRS的防控上,猪场需要良好的生物安全、科学的疫苗免疫与正确的药物方案。文章还探讨了在发生了PRRS感染的猪场,使用替米考星进行控制的效果。  相似文献   

16.
Pseudorabies virus (PRV) has been developed as a vaccine vector for expressing foreign immunogens. Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), continues to be a major problem to the pork industry worldwide. Many vaccine strategies have been developed to control the disease but most of them turn out to be unsuccessful. The objective of this research was to explore the feasibility of PRV-based vector vaccine in protection against PRRSV. A live attenuated vaccine-based PRV recombinant expressing the envelope protein GP5 of PRRSV was generated using recombinant DNA techniques. The Bartha-K61-derived recombinant virus, named rPRV-GP5, was shown to express PRRSV GP5 efficiently. Sixteen healthy piglets were assigned to one of four groups (one to four, four pigs per group). Animals in Groups 1 and 2 were each inoculated intramuscularly and intranasally with 10(7.0) PFU of rPRV-GP5 and its parent Bartha-K61, respectively; Group 3 were vaccinated intramuscularly with one-dose of PRRS inactivated vaccine; Group 4 was served as non-vaccinated control. One month later, all animals were all challenged with 10(6.5) TCID(50) of virulent PRRSV CH-1a. All animals in Groups 1 and 3 remained clinically healthy before and after challenge, with only a short period of fever (no more than 41 degrees C and 3 days), mild and gradually improving lung and kidney lesions, and short-term viremia (2 and 3 week, respectively) in spite of no detectable anti-PRRSV antibody before challenge. On the other hand, all animals in the other two groups showed evident clinical signs with higher temperatures (more than 41 degrees C) after challenge, and severe lung, kidney and spleen lesions and extended viremia (4 weeks). The results indicate that the rPRV-GP5 is safe for vaccinates and able to confer significant protection against clinical disease and reduce pathogenic lesions induced by PRRSV challenge in vaccinated pigs.  相似文献   

17.
Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), is globally the most economically important disease in commercial pigs, and novel control strategies are sought. This paper explores the potential to use host genetics to decrease the impact of PRRS on reproductive sows. Commercial pig data (7,542 phenotypic records) from a farm undergoing an outbreak of PRRSV were analyzed to assess the impact of PRRS on reproductive traits and the inheritance of such traits. First, differing methodologies were used to partition the data into time periods when the farm was disease free and when the farm was experiencing PRRSV outbreaks. The methods were a date/threshold method based on veterinary diagnosis and a threshold/threshold method based on trends in underlying performance data, creating the DTD and TTD data sets, respectively. The threshold/threshold method was more stringent in defining periods when PRRS was likely to be having an impact on reproductive performance, resulting in a data set (TTD) that was slightly smaller (1,977 litters from 1,526 sows) than that from the date/threshold method (3,164 litters and 1,662 sows), and it showed more pronounced impacts of PRRS on performance. Impacts on performance included significant increases in mean values of mummified and stillborn piglets (0.04 to 1.13 and 0.63 to 1.02, respectively) with a significant decrease in total born alive (10.3 to 9.08). Estimated heritabilities during the healthy phase were generally less (mummified piglets = 0.03 +/- 0.01, matings per conception = 0.04 +/- 0.01) than during the PRRSV outbreak (TTD data set; mummified piglets = 0.10 +/- 0.03, matings per conception = 0.46 +/- 0.04). These results imply genetic variation for host resistance to, or tolerance of, PRRSV, particularly with the TTD data set. Genetic correlations between reproductive traits measured in the healthy phase and TTD data set varied from effectively zero for traits describing numbers of mummified or dead piglets to strongly positive for litter size traits. This indicates genetic variation in piglet losses during PRRSV outbreaks is independent of genetic variation in the same traits in healthy herds. In summary, our findings show that there is within-breed genetic variation for commercially relevant traits that could be exploited in future breeding programs against PRRSV infection. Selection for increased PRRS resistance would be desirable to the industry because effective control measures remain elusive.  相似文献   

18.
选取健康长白二元杂交断奶仔猪18只，随机分为3组，即对照组、试验Ⅰ组、试验Ⅱ组。试验Ⅰ组注射猪繁殖障碍与呼吸道综合征疫苗，试验Ⅱ组注射猪繁殖障碍与呼吸道综合征疫苗之外，再注射板兰根多糖。再对试验猪进行猪繁殖与呼吸综合征病毒接种2．0mL／头。结果表明板蓝根多糖和猪繁殖障碍与呼吸道综合征疫苗联合使用，能提高PRRSV保护水平，两者联合应用，明显降低猪繁殖障碍与呼吸道综合征的发病率和死亡率。  相似文献   

19.
为掌握豫南地区规模化猪场中猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV2）疫苗免疫场猪群抗体水平和非疫苗免疫场猪群病原感染情况,试验利用ELISA方法对该地区猪群进行了PRRSV和PCV2抗体水平检测。结果表明,在疫苗免疫猪群中,PRRSV和PCV2抗体合格率分别为68.8%和58.7%,规模越大的猪场合格率越高,200~500头母猪规模场抗体阳性率最高（分别为76.8%和77.4%）,50头母猪以下场最低（分别为48.3%和44.2%）,种猪的合格率均最高,而育肥猪群合格率均最低。在非疫苗免疫猪群中,PRRSV和PCV2抗体阳性率分别为55.6%和65.3%,PRRSV抗体阳性率最低的是100~200头母猪规模场（46.5%）,最高的是50头母猪以下规模场（68.8%）,断奶仔猪和育肥猪群抗体阳性率均在71%以上;随猪场规模越大,PCV2抗体阳性率越低,200~500头母猪规模场抗体阳性率为41.4%,50~100头规模场和50头以下场的阳性率分别为78.9%和78.6%,种公、母猪的PCV2抗体阳性率最低（分别为37.5%和40.4%）,断奶仔猪和育肥猪抗体阳性率较高（分别为82.2%和79.5%）。本研究反映了豫南地区猪繁殖与呼吸综合征（porcine reproductive and respiratory syndrome,PRRS）和猪圆环病毒病（porcine circovirus disease,PCVD）疫苗免疫猪场的疫苗免疫效果和非疫苗免疫猪场病原感染的实际情况和规律,为该地区PRRS和PCVD防制工作提供了理论依据和指导。  相似文献   

20.
猪繁殖与呼吸综合征(PRRS)又称猪"蓝耳病",是由猪繁殖与呼吸综合征病毒(PRRSV)引起的以种猪群繁殖障碍,仔猪、生长育肥猪群呼吸道问题为特点,严重危害养猪业的重要病毒性疾病,给全世界养猪业造成了巨大的经济损失.自从2006年暴发高致病性PRRSV以来,PRRSV在国内不断变异演化,田间流行的毒株经常发生重组或变异...  相似文献   

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