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仔猪轮状病毒性腹泻的研究进展 总被引:2,自引:0,他引:2
仔猪轮状病毒性腹泻是由猪轮状病毒引起的急性肠道传染病,是危害养猪业的主要疾病之一。猪轮状病毒是一种条件性病原,一般情况下呈隐性感染,当环境因素发生变化、免疫力下降时可暴发。目前尚无有效治疗药物,预防该病的有效方法是加强饲养管理和有效疫苗接种。研制新一代高效、廉价疫苗是重要的研究方向。掌握仔猪轮状病毒性腹泻的动态变化和影响因素,可为其诊断以及疫苗的研制提供科学依据,并为该病发展趋势的科学预测提供数据和信息支持。文章综述了引起仔猪腹泻的猪轮状病毒的分群和分型、培养特性、流行病学、预防治疗及疫苗研制的现状。 相似文献
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猪轮状病毒病是由猪轮状病毒引起的急性肠道传染病,主要引起仔猪腹泻、呕吐和脱水,是危害养猪业的主要疾病之一。从该病的病原、诊断、防治等方面进行了综述,以期为科学防治该病提供理论依据。 相似文献
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猪轮状病毒病诊断与防制 总被引:2,自引:2,他引:0
猪轮状病毒病是由猪轮状病毒引起的急性肠道传染病,主要引起仔猪腹泻、呕吐和脱水,是危害养猪业的主要疾病之一.从该病的病原、诊断、防治等方面进行了综述,以期为科学防治该病提供理论依据. 相似文献
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<正>猪流行性腹泻病毒(PEDV)是引起猪腹泻的重要病原,主要引起两周龄以内仔猪发生以急性腹泻、脱水及高死亡率为特征的疾病,每年给各国养猪业造成了巨大经济损失。我国于1973年首次出现,1995年开始使用猪流行腹泻与猪传染性胃肠炎二联灭活疫苗和弱毒疫苗,其对该病的控制起到了积极的作用,但2010年10月以来,猪流行性腹泻在我国多个省发生,并且应用以往的疫苗,得不到有效的控制, 相似文献
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一、仔猪腹泻流行新特点(一)引起仔猪腹泻的疫病种类多1.细菌性疫病。主要包括仔猪黄痢、仔猪白痢、仔猪红痢、猪副伤寒、猪痢疾。2.病毒性疫病。主要包括猪流行性腹泻、传染性胃肠炎、轮状病毒感染、猪瘟、伪狂犬病、猪繁殖与呼吸综合征、猪圆环病毒病。3.寄生虫性疫病。主要是猪球虫病。(二)病原常呈混合感染,临床疫情复杂,影响诊断或引起诊断误差例如:传染性胃肠炎+猪流行性腹泻;传染性胃肠炎+猪流行性 相似文献
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猪腹泻是全球养猪生产中最常见的疾病之一,病毒性腹泻具有传播快、致死率高和防控难的特点。常见的引起仔猪腹泻的病毒包括猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)和猪轮状病毒(PoRV),近几年新发现的猪δ冠状病毒(PDCoV)、猪嵴病毒(PKV)和猪急性腹泻综合征冠状病毒(SADS-CoV)也与仔猪腹泻相关。病毒分离是病毒感染诊断的金标准,也是进行病毒研究的前提。对上述6种常见猪肠道病毒的分离进展进行综述,可为掌握这些病毒的分离情况、疾病发展动向监测、疫苗研发以及猪腹泻病的防控提供参考。 相似文献
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Midgley SE Bányai K Buesa J Halaihel N Hjulsager CK Jakab F Kaplon J Larsen LE Monini M Poljšak-Prijatelj M Pothier P Ruggeri FM Steyer A Koopmans M Böttiger B 《Veterinary microbiology》2012,156(3-4):238-245
Group A rotaviruses can infect both humans and animals. Individual rotavirus strains can occasionally cross species barriers and might hereby contribute to the emergence of new genotypes in heterologous hosts. The incidence and impact of zoonotic rotavirus are not well defined, and one reason for this is a lack of data about strains circulating in suspected reservoir animal hosts. In this study we report the incidence, genetic diversity, and molecular epidemiology of rotaviruses detected in domestic cattle and swine in 6 European countries. From 2003 to 2007, 1101 and more than 2000 faecal specimens were collected from swine and cattle, both healthy and diarrhoeic, and tested for rotaviruses. Viruses from positive stools were genotyped and a subset of strains was characterized by nucleotide sequencing and phylogenetic analysis of the VP7 (G) and VP4 (P) genes. Rotaviruses were detected in 43% of bovine samples and in 14% of porcine samples. In cattle, 10 different combinations of G and P types were identified and the most common strains were G6P[11] and G6P[5]. In swine, the number of identified G-P combinations was higher (n=21), however, no single combination was predominant across Europe. Newly described genotype specificities, P[27] and P[32], were identified in swine. When compared at the nucleotide sequence level, the identified porcine rotavirus strains and contemporary human strains grouped together phylogenetically, whereas bovine rotavirus strains formed separate clades. These data demonstrate large genetic diversity of porcine and bovine rotavirus strains across Europe, and suggest that livestock herds may serve as potential reservoirs for human infections. 相似文献
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本研究建立了可同时检测猪流行性腹泻病毒(Porcineepidemicdiarrheavirus,PEDV)、传染性胃肠炎病毒(TransmissiblegastroenteritisVirus,TGEV)、A群轮状病毒(GroupArotavirus,GARV)和猪嵴病毒(Porcinekobu—virus)的多重RT—PCR方法。检测中,建立的多重RT—PCR方法能够检测到500Pg的TGEV、PEDV、GARV和猪嵴病毒等量混合RNA模板,与常规的单一RT—PCR检测结果基本相同(检测TGEV、PEDV、GARV和猪嵴病毒的灵敏性分别为1000A、1000/6、93.33%和96.67%,特异性均为i00%)。结果表明,建立的多重RTPCR方法敏感性和特异性良好,可作为临床上猪病毒性腹泻病因快速、高效的诊断工具。应用该方法对2010—2012年华中地区190份腹泻仔猪样本进行检测,PEDV、TGEV、GARV和猪嵴病毒的阳性率分别为62.11%、0.53%、7.37%和82.11%。混合感染方面,PEDV和猪嵴病毒混合感染率为47.89%,PEDV和GARV混合感染率为4.74%,GARV和猪嵴病毒混合感染率为7.37%,PEDV、GARV和猪嵴病毒混合感染率为4.74%,未发现TGEV与其它3种病毒的混合感染情况。另外,有27份样本中仅检出PEDV(14.21%),57份样本只检出猪嵴病毒(30%)。分析表明,我国自2010年底大面积暴发的病毒性腹泻是多病原混合感染造成的,主要病原为PEDV,猪嵴病毒在其中所起作用尚待进一步验证和研究。 相似文献
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Two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. Viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. Successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. Porcine rotavirus passage in cell culture also was accomplished, using trypsin treatments in lieu of pancreatin treatments. Porcine rotavirus passaged 10 times in cell culture infected gnotobiotic pigs and caused diarrhea. Gnotobiotic pigs that recovered from this infection were resistant to challenge exposure with porcine rotavirus but were susceptible to challenge exposure with transmissible gastroenteritis virus. As determined by immunofluorescent cross reactions, porcine rotavirus was found to be antigenically related to the human and bovine rotaviruses but not to reovirus type 3 or to transmissible gastroenteritis virus. 相似文献
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猪传染性胃肠炎临床诊断及防治 总被引:1,自引:0,他引:1
猪传染性胃肠炎是由猪传染性胃肠炎病毒引起的急性高度接触性肠道传染病,临床上以腹泻、呕吐和脱水为特征,各种年龄的猪都可发病,对哺乳仔猪的危害最严重.然而以腹泻为主症的还有猪痢疾、仔猪黄痢、仔猪白痢、仔猪红痢(梭菌性肠炎)、流行性腹泻、轮状病毒感染、仔猪副伤寒等7种疫病,为了更有效地防控猪传染性胃肠炎,做到早诊断、早治疗.论文阐述了猪传染性胃肠炎与猪其他7种腹泻疫病的临床症状的区别及猪传染性胃肠炎的防治要点. 相似文献