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Adipose triglyceride lipase (ATGL) was recently identified and described as a major novel triglyceride lipase in animals. In this study, we aimed to study the tissue-specific and developmental expression pattern of porcine ATGL (pATGL) and the effect of resveratrol (RES) on expression of pATGL in vitro. The full-length cDNA sequence of pATGL was 1,958 bp (accession no. EF583921), with a 1,458-bp open reading frame encoding a 486-AA protein (the predicted molecular mass of 53.2 kDa, accession no. ABS58651). Comparison of the deduced AA sequence with the bovine, mouse, rat, dog, and human adipose triglyceride lipase showed 87, 84, 83, 81, and 80% similarity, respectively. Furthermore, the pATGL was highly expressed in porcine adipose tissue, to a lesser degree in kidney, heart, and muscle, and least but detectable in brain. In s.c. adipose tissue, pATGL mRNA was low at birth (1 kg of BW) and then increased, reaching a maximal value at 20 kg of BW (approximately 8 wk old; P < 0.01). In peritoneal and omental adipose tissue, the greatest expression of pATGL was observed at 40 kg of BW (approximately 12 wk old). In vitro, exposure of cultured adipocytes to 40 and 80 muM RES for 24 h increased the mRNA levels of pATGL by 95.3% (P < 0.05) and 146.8% (P < 0.01), respectively. Accordingly, lipid accumulation was decreased by 25.7% (P < 0.05) and 60.8% (P < 0.01), respectively. When treated with RES for 48 h, the mRNA levels of pATGL were increased by 104.1% (P < 0.05) and 163.1% (P < 0.01), respectively. As expected, lipid accumulation was decreased by 9.7% (P > 0.05) and 29.0% (P < 0.05), respectively. These results add to our understanding of the role of pATGL in adipose tissue development and as a potential target for regulating fat deposition and meat quality. 相似文献
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本试验旨在探究糖脂代谢通路关键基因CRTC3在不同品种猪肌肉和脂肪组织中的表达情况,并通过forskolin处理猪皮下脂肪前体细胞,研究forskolin对脂肪前体细胞分化聚酯和CRTC3基因表达的影响,阐明猪CRTC3基因表达与脂肪沉积的关系。试验选取杜长大猪和莱芜猪各5头,检测肌肉、脂肪组织中CRTC3的mRNA和蛋白表达水平以及脂肪代谢相关基因的mRNA表达水平;选取2头3日龄的杜长大仔猪,分离猪皮下脂肪前体细胞,待完全融合后用MDI诱导培养基诱导4 d,然后用分化培养基继续诱导4 d,完成诱导分化。Forskolin组在诱导分化的第1天即加入forskolin,使其终浓度为10μmol/L,对照组则加入同浓度的二甲基亚砜(DMSO)进行诱导分化。结果表明:在莱芜猪的背最长肌和腰大肌中,CRTC3的蛋白表达水平高于杜长大猪;在莱芜猪的皮下和内脏脂肪组织中,CRTC3及脂肪沉积相关基因过氧化物酶体增殖剂激活受体γ(PPARγ)、脂肪酸结合蛋白4(FABP4)、CCAAT/增强子结合蛋白α(C/EBPα)、围脂滴蛋白(PLIN)和瘦素(LEP)的mRNA表达水平显著或极显著高于杜长大猪(P<0.05或P<0.01),而脂肪棕色化相关基因NF-E2相关因子1(NRF1)、过氧化物酶体增殖物激活受体-γ共激活因子-1α(PGC⁃1α)、PRDM16、解偶联蛋白2(UCP2)、解偶联蛋白3(UCP3)的mRNA表达水平则显著或极显著低于杜长大猪(P<0.05或P<0.01)。进一步的研究发现,猪皮下脂肪前体细胞分化后CRTC3和脂肪沉积相关基因的mRNA表达水平极显著提高(P<0.01),脂肪棕色化相关基因的mRNA表达水平也均极显著升高(P<0.01)。10μmol/L forsko⁃lin处理能抑制猪皮下脂肪前体细胞分化,极显著升高环磷腺苷效应元件结合蛋白(CREB)和脂肪棕色化相关基因的mRNA表达水平(P<0.01),促进CRTC3的进核,极显著降低CRTC3和脂肪沉积相关基因的mRNA表达水平(P<0.01)。上述研究结果表明,CRTC3基因与猪脂肪沉积密切相关,forskolin处理可以调控猪CRTC3及脂质代谢相关基因表达,调控猪皮下脂肪前体细胞分化聚酯。 相似文献
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【目的】 扩增猪血清和糖皮质激素诱导型激酶（SGK）家族基因并进行生物信息学分析,探索其在猪脂肪组织和细胞中的表达模式。【方法】 以藏猪脂肪细胞cDNA为模板PCR扩增SGK家族基因,通过在线工具预测其编码蛋白的理化性质及亚细胞定位;用Mega X软件构建系统进化树;采集30日龄巴马猪心脏、肝脏、脾脏、肾脏、肺脏、背肌、腿肌、颈部脂肪、背部脂肪、腹股沟脂肪、肾周脂肪等组织及7日龄和4月龄猪腹股沟脂肪组织,通过实时荧光定量PCR检测SGK家族基因在猪不同部位组织中的表达;采集30日龄巴马猪腹股沟脂肪组织并分离基质血管成分（SVF）细胞,诱导SVF细胞向白色脂肪细胞分化,通过实时荧光定量PCR检测SGK家族基因及脂肪分化标记基因CCAAT增强子结合蛋白α（C/EBPα）、过氧化物酶体增殖物激活受体（PPARγ）在脂肪细胞中的表达。【结果】 SGK1、SGK2和SGK3基因CDS区序列长度分别为1 296、1 104和1 473 bp,分别编码431、367和490个氨基酸;SGK1和SGK2定位于细胞质,SGK3定位于细胞核,三者均为亲水性蛋白,3个蛋白均含有相同基序,保守性高;系统进化树结果表明,猪与牛的亲缘关系最近;SGK1和SGK3基因在心脏、肝脏、脾脏、肺脏、肾脏、多种肌肉及脂肪组织广泛表达,SGK2基因在颈部、背部、腹股沟、肾周脂肪组织中均有较高表达;SGK1和SGK2基因在7日龄猪脂肪组织中表达量极显著高于4月龄猪脂肪组织（P<0.01）,SGK3基因在4月龄和7日龄的猪脂肪组织中表达量无显著差异（P>0.05）,且SGK3基因的表达量低于SGK1和SGK2基因;与未分化脂肪细胞相比,在分化后的脂肪细胞中SGK1和SGK2基因的表达量极显著上调（P<0.01）,且SGK1基因的表达量远高于SGK2基因,而SGK3基因的表达量无显著变化（P>0.05）。【结论】 SGK家族蛋白具有保守结构域,可能发挥着相似的功能,SGK1和SGK2可能参与调控猪脂肪细胞的分化过程,结果可为探究猪脂肪沉积的分子机制提供一定的理论基础。 相似文献
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The relationship between leptin mRNA and insulin status was explored using streptozotocin diabetic pigs. Twelve male Yorkshire x Landrace crossbred swine (approximately 40 kg BW) were divided into three groups. Two groups were rendered diabetic with the use of streptozotocin (75 mg/kg BW). Diabetes was confirmed 24 h after streptozotocin treatment by the presence of hyperglycemia. One group of diabetic animals received daily injections of insulin (.5 U/(kg x d)(-1)) for 7 d, whereas the other group of diabetic animals received saline injections. The nondiabetic group also received saline injections (controls). Tissue and blood were collected after 7 d of treatment. Leptin mRNA concentrations in dorsal s.c. adipose tissue were measured by Northern analysis and standardized against 28S rRNA expression. Diabetes reduced leptin mRNA concentration by 67% in s.c. adipose tissue (P < .05). Serum insulin concentrations in the diabetic animals were reduced by 69% (P < .05). Insulin treatment of diabetic animals resulted in an increase in leptin mRNA concentration to levels in controls. Primary cell culture of porcine adipose tissue was used to assess whether these actions were the direct or indirect action of insulin. Acute exposure (1 to 24 h) of primary cultures of porcine adipocytes to insulin did not result in a change in leptin expression. However, chronic (7-d) exposure to insulin elevated leptin mRNA levels by 73%. These data suggest that insulin mediates changes in porcine leptin mRNA levels in vivo or in vitro, most likely by an indirect action. 相似文献
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利用半定量RT-PCR法分析比较了甘油三酯水解酶(Triacylglycerol hydrolase,TGH)和激素敏感脂酶(Hormone-sensitive lipase,HSL)基因在不同猪种、不同发育阶段及不同部位脂肪组织中转录表达的差异,探讨其在猪脂肪组织的表达规律。结果显示,脂肪型个体TGHmRNA表达丰度显著低于瘦肉型和杂交型个体,成年猪较初生仔猪低,皮下、腹膜和内脏脂肪组织中TGH表达量依次递增;其变化规律与HSL相同。此外,对分离培养的原代前体脂肪细胞通过诱导分化和油红O染色区分分化状态,分析TGHmRNA表达的时序变化,发现TGH在前脂肪细胞中不转录表达,诱导分化后开始表达,且在诱导分化第4天表达量最高,分化第10天表达量下降,达到峰值的时间较HSL早。结果表明,TGH的表达与个体肥胖程度、年龄、脂肪组织部位以及脂肪细胞分化程度相关,同时,在脂肪细胞分化过程中,TGH表达峰值早于HSL,提示TGH在脂肪细胞发育过程中可能较早承担基础脂解作用。 相似文献
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本研究通过构建腺病毒介导的体外超表达载体，探究腺苷甲硫氨酸转移酶2A（methionine adenosyltransferase 2A，MAT2A）基因在猪肌内脂肪细胞分化中的作用。根据GenBank中猪MAT2A基因mRNA序列（登录号：NM_001167650.1）设计引物，提取猪脂肪组织细胞总RNA并反转录获得cDNA，以此为模板进行PCR扩增并连接到pAdTrack-CMV腺病毒穿梭载体中，对重组质粒pAd-MAT2A进行测序鉴定；pAd-MAT2A载体经PacⅠ限制酶酶切线性化，经质粒大片段回收纯化后转染293A细胞进行病毒包装；采用实时荧光定量PCR检测MAT2A基因表达情况，并提取蛋白进行Western blotting分析，取分化第8天的细胞进行油红O染色。结果表明，穿梭载体pAdTrack-CMV-MAT2A构建成功，并能与骨架载体pAdEasy-1实现同源重组；腺病毒载体pAd-MAT2A转染293A细胞后，病毒滴度达到1E+6 PFU/mL，可满足侵染猪肌内脂肪细胞的需要。实时荧光定量PCR和Western blotting结果显示，MAT2A基因mRNA和蛋白水平均显著上调。油红O染色结果显示，过表达MAT2A基因可促进猪肌内脂肪细胞内脂滴聚积。结果表明，腺病毒介导的MAT2A基因过表达在猪肌内脂肪细胞中呈上调趋势，MAT2A基因可促进脂质积累。 相似文献
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旨在探讨NR1H3基因在猪脂肪组织中的发育性表达规律及对猪前体脂肪细胞成脂分化的影响,以确定其在脂肪沉积过程中的主要功能。本研究采用qRT-PCR方法检测30、90、240日龄马身猪皮下脂肪组织中NR1H3的发育性表达规律;采集5日龄杜长大仔猪背部脂肪组织,分离猪前体脂肪细胞;通过细胞免疫荧光技术检测细胞中Adiponectin含量以鉴定细胞的纯度;构建猪NR1H3基因的过表达载体,设计NR1H3 siRNA序列,分别转染分离得到的猪前体脂肪细胞,采用qRT-PCR、Western blot和油红O染色等方法检测过表达和干扰效率及它们对成脂分化关键基因表达的影响。结果表明,马身猪皮下脂肪组织中NR1H3的表达量随日龄增加呈上升趋势,30日龄时表达量最低,240日龄时表达量最高,差异极显著(P<0.01)。与对照组相比,猪前体脂肪细胞中过表达NR1H3,脂肪细胞的脂滴数明显增多,下游靶标SREBP-1c和ChREBP的表达量极显著提高(P<0.01),且成脂关键基因FAS、C/EBPβ、PPARγ和FABP4的mRNA表达量极显著提高(P<0.01),促进成脂过程;相反,干扰NR1H3基因,脂滴数明显减少,下游靶标及成脂关键基因的mRNA表达量极显著下调(P<0.01),抑制成脂过程。本研究表明,NR1H3基因是猪前体脂肪细胞成脂分化的正调节剂,通过影响其下游靶标SREBP-1c和ChREBP的表达而影响成脂分化,研究结果对阐明猪脂肪沉积的分子机理、改善肉质品质有重要意义。 相似文献
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Cloning, expression and chromosome localization of porcine adiponectin and adiponectin receptors genes 总被引：7，自引：0，他引：7
Dai MH Xia T Zhang GD Chen XD Gan L Feng SQ Qiu H Peng Y Yang ZQ 《Domestic animal endocrinology》2006,30(2):117-125
Adiponectin is a cytokine secreted specifically by adipocytes that has been proposed to enhance insulin sensitivity and prevent atherosclerosis. Adiponectin receptors (adipoR1 and adipoR2) are recently found in mice which act as receptors for globular and full-length adiponectin to mediate the fatty-acid oxidation and glucose uptake in muscle and liver. The primary goal of this study was to examine chromosome localization of porcine adiponectin and adiponectin receptors and the gene expression pattern in various tissues of pigs of the three genes. Radiation hybrid mapping demonstrated that porcine adiponectin, adipoR1 and adipoR2 were located to chromosome13q36-41, 10p11 and 5q25, in the regions that were syntenic to the homologs of human genes, respectively. Semi-quantitative RT-PCR showed that porcine adiponectin mRNA was specifically expressed in adipose tissue and porcine adipoR1 and adipoR2 mRNA were ubiquitously expressed in many tissues except brain. Comparison to adipoR2 mRNA which was highly expressed in liver, heart, kidney, adipose tissues and lung, adipoR1 mRNA was expressed at relatively high levels in porcine muscle, leukocytes and epididymis. Our data provide basic molecular information useful for the further investigation on the function of the three genes. 相似文献
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The purpose of this study was to detect differential expression of genes related to adipocyte differentiation in pigs by suppression subtractive hybridization. Adipocytes and stromal vascular cells (a fraction containing preadipocytes) from pig adipose tissue were isolated for mRNA extraction. The cDNA from preadipocytes was subtracted from the cDNA from adipocytes. The subtracted gene fragments were cloned into pGEM-T Easy TA cloning vector. We selected 384 clones for gene sequence determination and for further analysis. These genes were subjected to a differential screening procedure to confirm the differential expression of genes between the 2 cell types. We found that at least 36 genes were highly expressed in the adipocytes compared with preadipocytes. Among these, 6 genes including 2 novel genes with the greatest differences were selected and confirmed by Northern analysis. We found that angiotensin I-converting enzyme (ACE), ataxia-telangiectasia mutated protein (ATM), calpain 1, and stearoyl coenzyme A desaturase 1 (SCD1) were highly expressed in adipocytes compared with preadipocytes (P < 0.05). The relative mRNA abundance of ACE, ATM, calpain 1, SCD1, and 2 novel genes discovered in the current study was increased at the later stages of adipocyte differentiation (P < 0.05). The results confirmed that the genes involved in lipid metabolism and adipocyte differentiation were highly expressed in porcine adipocytes. However, further investigation is needed to demonstrate specific functions of the novel genes discovered in the current study. 相似文献
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Smith SB Hively TS Cortese GM Han JJ Chung KY Casteñada P Gilbert CD Adams VL Mersmann HJ 《Journal of animal science》2002,80(8):2110-2115
Conjugated linoleic acid (CLA) has been shown to have an effect on subcutaneous fatty acid composition and has been reported to decrease stearoyl coenzyme A desaturase (SCD) activity by decreasing mRNA expression and(or) catalytic activity in rodents and rodent cell lines. This investigation was designed to study the effects of CLA, corn oil, or beef tallow supplementation on s.c. adipose tissue fatty acid composition, adiposity, SCD enzyme activity, and the delta9 desaturase index in piglets. Eighteen crossbred barrows 16 to 18 d of age were adapted to diet for 1 wk and then assigned randomly to one of three treatments: 1.5% added CLA, 1.5% added corn oil, or 1.5% added beef tallow. Barrows were penned individually and fed the supplemental oils for 35 d (to 25.6 +/- 0.6 kg BW). Subcutaneous adipose tissue samples were obtained after slaughter. Fatty acid composition of the s.c. adipose tissue differed for each fatty acid measured due to diet with the exception of 18:3. The concentrations of CLA trans-10, cis-12 and cis-9, trans-11 were elevated from nondetectable to 1.62 and 2.52 g/100 g lipid, respectively (P < 0.001 for both isomers). Conjugated linoleic acid decreased the delta9 desaturase index (P < 0.01) and SCD enzyme activity, expressed as nanomoles of palmitate converted to palmitoleate/(7 min x g of tissue) (P = 0.075) and nanomoles of palmitate converted to palmitoleate/(7 min 105 cells) (P= 0.056). Tallow-fed pigs had a greater proportion of large adipocytes (> 700 pL) and the greatest SCD activity. These data provide the first direct evidence that dietary CLA depresses SCD enzyme activity in porcine adipose tissue, which may in part be responsible for the depression of adiposity by CLA observed by others in market weight pigs. 相似文献
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The aim of the study was to investigate the effects of dietary linseed (rich in n-3 PUFA) on expression of inflammation-related genes and on growth performance of growing-finishing barrows. Two isoenergetic and isonitrogenous diets were formulated, one as the basal diet and the other containing 10% linseed. Twenty-four Landrace x Yorkshire barrows weighing 35 +/- 3.7 kg were randomly assigned to 1 of 4 treatment groups, with 6 pigs per group. During the entire experimental period of 90 d, these 4 groups of pigs were first fed the basal diet and then fed the linseed diet for 0, 30, 60, and 90 d before slaughter, respectively. Pig growth; messenger RNA (mRNA) expression of peroxisome proliferator-activated receptor-gamma (PPARgamma), IL-1beta0, IL-6, and tumor necrosis factor-alpha (TNF-alpha); and plasma concentrations of the 3 proinflammatory cytokines were measured and analyzed. Average daily feed intake did not differ among treatment groups (P > 0.05), but ADG (P < 0.05) and G:F (P < 0.01) responded quadratically to the duration of linseed diet feeding, and pigs in the 60-d treatment group had the greatest ADG and G:F. The mRNA expression of PPARgamma in loin muscle and spleen increased linearly (P < 0.01) with the duration of linseed diet feeding, whereas its expression in adipose tissue was not affected (P = 0.095). Tumor necrosis factor-alpha and IL-6 mRNA expression in muscle, adipose, and spleen, as well as serum concentration of TNF-alpha, decreased linearly (P < 0.01) with the duration of linseed diet feeding. Peroxisome proliferator-activated receptor-gamma mRNA abundance was negatively correlated with IL-1beta, IL-6, and TNF-alpha mRNA abundance both in muscle (R(2) = 0.63, P < 0.001) and in spleen (R(2) = 0.69, P < 0.001), and PPARgamma mRNA expression in spleen (R(2) = 0.59, P < 0.01) and muscle (R(2) = 0.52, P < 0.05) was negatively correlated with serum TNF-alpha concentration. There were also significant quadratic relations between ADG and expression of PPARgamma (P < 0.05) and splenic TNF-alpha (P < 0.05). These data suggest that intake of n-3 PUFA from the linseed diet led to significant decreases in the expression of proinflammatory cytokine genes, which may stimulate growth in growing-finishing barrows, at least in part, through a PPARgamma-dependent mechanism. 相似文献
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Ghinis-Hozumi Y González-Gallardo A González-Dávalos L Antaramian A Villarroya F Shimada A Varela-Echavarría A Mora O 《Journal of animal science》2011,89(8):2529-2536
Sirtuins, the mammalian homologs of the silent information regulator 2 gene of Saccharomyces cerevisiae, are members of the NAD(+)-dependent family of histone deacetylases. In vertebrates, 7 sirtuins have been described, with different cellular localizations and target proteins. Glucose and lipid metabolism are among the processes regulated by these enzymes. In ruminants, gluconeogenesis is the main biochemical pathway by which glucose is obtained. Because sirtuins in bovines have not been studied, the aim of this work was to obtain sequences coding for the 7 sirtuins and determine the expression patterns of sirtuin1 (Sirt1) and sirtuin3 (Sirt3) in the liver, muscle, and adipose tissue of calves and bulls. Using PCR amplification, we obtained sirtuin gene sequences and reported them to the National Center for Biotechnology Information GenBank. Characteristic sequence motifs corresponding to the sirtuin catalytic core domain were found, including the active and zinc-binding sites. Relative expression patterns of Sirt1 and Sirt3 in liver, muscle, and adipose tissue were quantified by real-time PCR, normalizing to the geometric mean of the housekeeping genes cyclophilin A and β-actin. Expression of Sirt1 was less in liver and muscle, whereas it was greater in adipose tissue of adult animals, with statistical differences (P=0.0071) only in the latter. In the case of Sirt3, expression was greater in all 3 adult tissues, but statistical differences were found only in liver (P=0.0141) and muscle (P=0.0017). The greatest expression was observed in liver for Sirt1 and in muscle for Sirt3, whereas the least expression was in muscle for Sirt1 and in adipose tissue for Sirt3. In other species, sirtuin expression (both Sirt1 and Sirt3) in liver is reported to be the greatest among these 3 tissues, a pattern different from what we measured. These differences in expression can be associated with metabolic differences between nonruminant and ruminant species. However, further research on the relationship between bovine sirtuins and ruminant metabolism is required for a better understanding of these fields. 相似文献
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Two experiments were conducted to determine the effects of anabolic implants on performance, changes in ultrasound measurements, carcass quality, cellularity of i.m. and s.c. adipose depots, and mRNA expression of acetyl CoA carboxylase (ACC), stearoyl CoA desaturase (SCD), and lipoprotein lipase (LPL) in i.m. adipose tissue of finished beef cattle. Angus heifers (experiment 1: n = 10; 411 kg of BW) and steers (experiment 2: n = 18; 279 kg of BW) were randomly allotted as control (C) or implanted with Synovex-Plus (SP) at d 0 and midway through the finishing period. The cattle were fed a high-concentrate diet and were weighed at approximately 28-d intervals. Heifers and steers were finished for 108 and 133 d, respectively. At slaughter, a section of the LM (sixth to ninth rib) was removed, and i.m. adipose tissue was dissected for mRNA analysis. Subcutaneous and i.m. adipose tissues also were collected for determination of cellularity. At 48 h postmortem, carcass data were collected, and a steak (12th rib) was removed for analysis of lipid and fatty acid composition. Body weight did not differ (P > 0.10) between treatments until after reimplanting of the heifers (d 55) or steers (d 73). Average daily gain was 36 and 16% faster (P < or = 0.01) for implanted heifers and steers, respectively, compared with their control counterparts. Implanting resulted in larger (P < or = 0.10) HCW and LM area for heifers and steers. However, implanting did not affect (P > 0.10) dressing percent, fat thickness, percentage of KPH, yield grade, or marbling score. Intramuscular lipid content and concentrations of major fatty acids did not differ (P > 0.10) between treatments. Percentage of SC adipocytes was greater at larger diameters ( > 150 microm), whereas the majority of i.m. adipocytes were at small to middle diameters (50 to 150 microm). The number of i.m. adipocytes per gram of tissue was greater (P < 0.05) for SP than C and also were greater (P < 0.05) than the number of s.c. adipocytes in SP heifers. In experiment 2, adipocytes per gram of tissue tended to be greater (P = 0.07) for SP than C and were greater (P < 0.01) for i.m. than s.c. In experiment 1, average cell diameter and volume did not differ (P > 0.10) between treatments and tissues, but in experiment 2 both cellularity traits were greater (P < 0.01) for s.c. than for i.m.. Implanting did not alter mRNA expression of ACC, SCD, or LPL in i.m. adipose tissue. This study shows that anabolic implants do not appear to have direct effects on i.m. lipid deposition. 相似文献
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本试验旨在研究植物提取物白藜芦醇(RES)对牛皮下脂肪细胞凋亡率以及去乙酰化酶1(SIRT1)/腺苷一磷酸激活的蛋白激酶(AMPK)信号通路关键因子的mRNA和蛋白质表达量的影响。选取18月龄鲁西黄牛的皮下前体脂肪细胞,在细胞分化的第0天,更换为RES浓度分别为0(对照)、100、200和400μmol/L的培养液处理48 h,每组设3个重复。应用Hoechst33342染色检测细胞凋亡的形态学变化,流式细胞分析仪检测细胞凋亡率,荧光定量PCR(q PCR)和免疫印迹试验(Western-blot)分别检测SIRT1/AMPK信号通路上的关键因子SIRT1、A MPKα、叉头转录因子1(Fox O1)、B细胞淋巴瘤/白血病-2(Bcl-2)、半胱氨酸天冬氨酸蛋白酶-3(caspase-3)、促凋亡蛋白Bcl-2相关X蛋白(Bax)的mRNA和蛋白质表达量,并利用油红O染色鉴定脂肪细胞。结果表明,与对照组相比:不同浓度RES处理后的牛皮下脂肪细胞凋亡率均极显著增高(P0.01);不同浓度RES处理后的SIRT1、AMPKα、caspase-3、Bax的mRNA表达量均显著或极显著提高(P0.05或P0.01),Bcl-2的mRNA表达量极显著降低(P0.01);不同浓度RES处理后的SIRT1、AMPKα、caspase-3的蛋白质表达量均显著或极显著提高(P0.05或P0.01);200和400μmol/L RES处理后,Fox O1的mRNA和蛋白质表达量显著或极显著提高(P0.05或P0.01),Bax的蛋白质表达量极显著提高(P0.01),Bcl-2的蛋白质表达量极显著降低(P0.01)。100μmol/L RES处理后时,Fox O1的mRNA和蛋白质表达量以及Bcl-2、Bax的蛋白质表达量均与对照组差异不显著(P0.05)。由此可见,RES通过激活SIRT1/AM PK信号通路,同时激活通路下游的Fox O1,促进了牛皮下脂肪细胞的凋亡,为通过营养调控技术降低牛皮下脂肪沉积提供了一定的理论基础。 相似文献
19.
大豆异黄酮抵抗体外培养猪脂肪细胞氧化损伤的作用 总被引：2，自引：0，他引：2
为了探明大豆异黄酮对猪脂肪细胞氧化损伤的保护效应,试验分别用含0、10、20、40μmol/L和80μmol/L异黄酮S(一种合成的大豆异黄酮)或三羟基异黄酮(GEN)的完全培养液培养猪脂肪细胞48h,用终浓度为100μmol/L的FeSO4和H2O2溶液进行氧化处理1 h。结果表明:与正常对照组相比,氧化对照组细胞内活性氧(ROS)水平和脂质过氧化产物丙二醛(MDA)含量分别升高了1.61倍和2.74倍(P<0.01);与氧化对照组相比,添加10、20、40μmol/L和80μmol/L异黄酮S使细胞内ROS水平分别下降了24.62%(P<0.05)、20.03%(P<0.05)、37.88%(P<0.01)和41.20%(P<0.01);添加10、20、40μmol/L和80μmol/L GEN均显著降低了细胞内ROS水平(P<0.01)和MDA含量(P<0.05)。试验结果提示在本试验条件下,异黄酮S和GEN能通过抑制猪脂肪细胞的脂质过氧化、降低ROS产生,抵抗羟自由基对猪脂肪细胞的氧化损伤。 相似文献