共查询到19条相似文献，搜索用时 210 毫秒
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实验旨在探究不同浓度的Mito-tempo对绵羊精液低温保存效果的影响。采集8只湖羊种公羊精液，分别用含0、15、30、45、60μmol/L Mito-tempo稀释液进行8倍稀释，5℃冷藏120 h。分别在24 h和120 h检测精液品质指标（活力、活率、平均路径速度、曲线运动速度、直线运动速度、质膜完整性、顶体完整性、DNA完整性和线粒体活性）及抗氧化指标（总抗氧化能力、超氧化物歧化酶活性和丙二醛含量）。结果表明：在低温保存期间，30μmol/L Mito-tempo处理组精子活力、活率、平均路径速度、DNA完整性、顶体完整性、线粒体活性、超氧化物歧化酶含量和总抗氧化能力均高于对照组及60μmol/L Mito-tempo处理组（P<0.05）；在低温保存过程中，30μmol/L Mito-tempo处理组丙二醛含量低于对照组和其余各试验组（P<0.05）。由此可见，在稀释液中添加30μmol/L Mito-tempo可有效提高低温保存后绵羊精液质量。 相似文献
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本实验旨在探究还原型谷胱甘肽（GSH）对西门塔尔牛精液低温保存（0℃）的影响。在精液稀释液中分别添加0（对照组）、1、2、4、6 mmol/L的GSH,在0℃条件下保存24、72、120、168、216 h时检测精子活力、顶体完整率和质膜完整性等指标;保存72 h和168 h时检测精子的丙二醛（MDA）水平、三磷酸腺苷（ATP）含量、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性。结果表明：在精液低温保存72 h时,4 mmol/L GSH添加组的精子活力、顶体完整率、质膜完整性、CAT活性、ATP含量和GSH-Px活性高于对照组（P<0.05）;在精液低温保存168 h时,4 mmol/L GSH添加组的精子活力、顶体完整率、质膜完整性、CAT活性、ATP含量和GSH-Px活性高于对照组与其他GSH添加组（P<0.05）,MDA水平低于其他组（P<0.05）。因此,在本实验条件下,在精液稀释液中添加4 mmol/L GSH可以有效提高精液品质和精子的抗氧化能力,延长精子的保存时间。 相似文献
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含N-乙酰半胱氨酸稀释液对鸡精液低温保存效果的影响 《畜牧与饲料科学》2016,37(8):27-27
采集安卡红种公鸡鲜精,分别用含不同浓度N-乙酰半胱氨酸的A、B 2种稀释液稀释后低温保存,之后测定保存时间、生存指数和精子畸形率,比较不同浓度的N-乙酰半胱氨酸对鸡精液在低温保存下的效果。结果表明：A液中,0.013 0 mol/L添加量和0.002 6 mol/L添加量相比保存时间和生存指数均无显著差异（P〉0.05）,且均极显著（P〈0.01）高于A液无添加组;B液中,0.013 0 mol/L添加量在保存时间和生存指数方面均极显著（P〈0.01）优于0.002 6 mol/L添加量和无添加组,0.002 6 mol/L添加量与B液无添加组相比,保存时间和生存指数差异均不显著（P〉0.05）。而2种稀释液中是否添加N-乙酰半胱氨酸及添加量的多少对精子畸形率影响均不大（P〉0.05）。 相似文献
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试验比较了在冷冻稀释液中添加不同浓度海藻糖(0.025,0.05,0.1 mol/L)以及添加10 mg/L维生素C对猪精液冷冻保存效果的影响.结果:(1)添加0.025 mol/L海藻糖组的精子冻后活率与复苏率分别跟对照组相比无显著差异(P>0.05),但添加浓度为0.05,0.1 mol/L的海藻糖组的精子活率和复苏率显著低于对照组(P<0.05).(2)在猪精液冷冻保存稀释液中,添加维生素C组的精子冻后活率和复苏率极显著高于对照组(P<0.01).表明在猪精液冷冻液中,添加0.025,0.05,0.1 mol/L 3种不同浓度的海藻糖对猪精液没有起到保护作用,其中后两者对之有降低的作用;而添加10 mg/L维生素C可取得较好的冷冻保存效果. 相似文献
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黄芪多糖对猪精液低温保存的影响 总被引：1，自引：0，他引：1
通过在改进的猪精液低温稀释液中添加不同质量浓度的黄芪多糖(0、0.10、0.20、0.30、0.40g/L),对比7d内、4℃低温保存下猪精子的活率、活力、精子畸形率、精子质膜和顶体的完整性等参数,探讨黄芪多糖在猪精液低温保存应用的效果。结果显示,在猪精液低温保存后的质量上,0.10g/L质量浓度组与对照组差异不显著(P0.05),0.40g/L质量浓度组显著差于对照组(P0.05),0.20、0.30g/L质量浓度组显著好于对照组(P0.05),所有各组中0.30g/L质量浓度组效果最好。结果表明,在本试验条件下,适宜质量浓度的黄芪多糖可以明显改善4℃低温条件下猪精液的保存效果,其中最佳添加质量浓度是0.30g/L。 相似文献
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为研究原花青素（PC）对吐鲁番黑羊精液低温保存效果的影响,本实验采用假阴道法采集5只2岁成年吐鲁番黑羊公羊精液,在精液基础稀释液中添加不同浓度（0、10、20、30、40μg/mL）的PC进行低温保存,每隔24 h检测精子活率、活力、曲线速率（VCL）、直线速率（VSL）、畸形率、质膜完整率等指标,在保存24、72、120 h时检测总抗氧化能力（T-AOC）活性和过氧化氢酶（CAT）活性。结果表明：各PC添加组的精子活率、活力、VCL、VSL、质膜完整率、T-AOC和CAT活性均高于对照组（P<0.05）,畸形率低于对照组（P<0.05）,其中20μg/mL PC添加组的精子质量高于其他各组（P<0.05）。综上所述,在基础稀释液中添加PC可以显著提高精液品质,且PC添加浓度为20μg/mL时对吐鲁番黑羊精液低温保存效果最佳。 相似文献
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稀释液中添加海藻糖对猪冷冻精液质量参数和活性氧的影响 总被引：8，自引：2，他引：8
本试验旨在探讨海藻糖对猪精子冷冻效果以及精子内活性氧(ROS)的含量影响。试验分对照组和4个海藻糖处理组(0.025,0.05,0.1和0.2 mol/L)。精子冷却后,添加0.1 mol/L海藻糖处理组精子活力显著(P<0.05)高于对照组,而精子内和稀释液中各组间ROS发生量都没有显著的差异。精子冷冻解冻后,添加0.05 mol/L海藻糖处理组的精子活力显著(P<0.05)高于对照组;添加0.05 mol/L和0.1 mol/L海藻糖处理组的精子生存率显著(P<0.05)高于对照组;添加0.1 mol/L海藻糖处理组顶体完整的精子百分比显著(P<0.05)高于对照组;4个海藻糖处理组膨胀精子百分比都显著(P<0.05)高于对照组;添加0.025 mol/L海藻糖处理组ROS水平显著(P<0.05)低于对照组。结果表明:海藻糖对精子冷冻保存是有益的,且能抑制精子内ROS的发生,但它的作用机制有待于进一步的研究。 相似文献
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试验旨在研究不同抗氧化剂对马精子低温保存及冷冻效果的影响。选取6匹英纯血种公马作为试验动物,以INRA82液作为基础稀释液（对照组）,分别添加谷氨酰胺（0.015 g/mL）、甘氨酸（0.019 g/mL）、半胱氨酸（0.024 g/mL）、甲硫氨酸（0.015 g/mL）、牛磺酸（0.063 g/mL）、维生素C（0.4 mg/mL）、维生素E（0.5 mg/mL）、褪黑素（0.001 mg/mL）制备成含有不同抗氧化剂的低温保存和冷冻保存稀释液,将浓缩处理后的马精子分别置于上述稀释液中保存或冷冻,检测低温保存48 h后精子运动参数,评价抗氧化剂对精子低温保存的影响;检测精子冻融后运动参数、精子质膜完整性、线粒体膜电势及顶体完整性,评价抗氧化剂对精子冷冻效果的影响。结果表明,精子低温保存48 h后,添加牛磺酸组活精子比例和前向运动精子比例显著高于对照组（P<0.05）,添加维生素C组前向运动精子比例显著高于对照组（P<0.05）。冷冻解冻后结果表明,添加不同抗氧化剂没有改善精子冻融后活精子比例和前向运动精子比例,但添加甲硫氨酸显著延长了精子体外存活能力（P<0.05）;添加不同抗氧化剂精子质膜完整性与对照组间无显著差异（P>0.05）,但甲硫氨酸和甘氨酸组有高于对照组的趋势;添加维生素E和甲硫氨酸组精子线粒体膜电势显著高于对照组（P<0.05）,不同抗氧化剂组精子顶体完整性与对照组间均无显著差异（P>0.05）。结果提示,低温保存时添加牛磺酸和维生素C可以提高精液低温保存效果;冷冻时添加甲硫氨酸能提高精子质膜完整性和线粒体膜电势,且能够延长精子冻融后的存活时间。 相似文献
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海藻糖和甘油相互协同提高绒山羊精液冷冻保存品质 总被引：1，自引：0，他引：1
本实验旨在研究不同浓度的海藻糖和甘油相互协同对绒山羊精液冷冻保存的影响。将0 mmol/L (T0)、50mmol/L(T50)的海藻糖和0%(G0)、1%(G1)、2%(G2)、3%(G3)的甘油分别组合为T0G0、T0G1、T0G2、T0G3、T50G0、T50G1、T50G2和T50G3。以Tris-柠檬酸-葡萄糖(TCG)为基础稀释液,分析不同组合对绒山羊精液冷冻保存的影响。检测解冻后精子活力、运动参数、DNA完整率、顶体、质膜完整率、抗氧化水平。结果表明:海藻糖和甘油联合添加时精子冷冻保存效果显著高于单独添加海藻糖或甘油组,其中T50G1组作用最显著。在基础稀释液中添加50 mmol/L(T50)的海藻糖时,精子冷冻解冻后的活力、质膜完整率随着甘油浓度的升高而降低,而在基础稀释液中不添加海藻糖时,精子冷冻解冻后的活力、质膜完整率随着甘油浓度的升高而升高。综上表明,海藻糖和甘油通过协同作用提高精子冷冻保存效果,两者发挥作用的最适浓度为50 mmol/L海藻糖和1%甘油,并且海藻糖和甘油协同作用对精子活力以及质膜完整性具有浓度依赖性。 相似文献
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为了探讨添加不同水平L-赖氨酸的精液稀释液对杜泊羊鲜精液态保存下品质的影响,试验选用健康的杜泊种公羊3只,采集精液,等量分装,分别加入到含不同水平(0、0.1、0.2、0.3、0.4和0.5 g) L-赖氨酸的120 mL稀释液中,在0、6、24、30、48、54、72和78 h对精子活力、质膜完整率和顶体完整率进行检测。结果表明,添加0、0.1、0.2 g L-赖氨酸组精子活力较其他添加水平组高,添加0.4和0.5 g L-赖氨酸组精子活力下降速度快,添加0.5 g L-赖氨酸组精子活力在30 h时降为0,添加0.4 g L-赖氨酸组精子活力在48 h时降为0;添加0.1 g L-赖氨酸组精子有效存活时间和生存指数最高,与其他组间差异显著(P< 0.05);质膜完整率0.1 g组最高,贮存0~54 h间与对照组间差异不显著(P >0.05),72 h后与对照组间出现显著性差异(P< 0.05),0.4和0.5 g组最差,与其他组间差异显著(P< 0.05);顶体完整率0.1 g组最高,与对照组间差异不显著(P >0.05),0.4和0.5 g组最差,二者差异不显著(P >0.05),除贮存0和6 h外,与其他组间差异极显著(P< 0.01)。结果提示,稀释液中添加高浓度的L-赖氨酸对精液品质有抑制作用,当L-赖氨酸添加水平≥0.2 g,精子品质显著下降,添加0.1 g L-赖氨酸有助于提高杜泊羊鲜精液态保存的品质。 相似文献
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MAO Ran LI Qing-quan SHI Yan LAN Xiang-li WANG Min ZONG Ze-jun XIA Guo-zhong HAN Tian-long 《中国畜牧兽医》2015,42(12):3308-3314
In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state. 相似文献
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探讨了猪精子冷冻保存液中添加不同浓度的海藻糖(trehalose)和EDTA(0.05mol/L trehalose、0.05mol/L trehalose 2.0mmol/LEDTA、0.05mol/L trehalose 5.0mmol/L EDTA、0.1mol/L trehalose、0.1mol/L tre-halose 2.0mmol/LEDTA、0.1mol/L trehalose 5.0mmol/LEDTA)对猪精子冷冻效果的影响。结果表明,精子冷冻解冻后,0.1mol/Ltrehalose 2mmol/LEDTA混合处理组精子活力显著高于0.1mol/Ltrehalose处理组(P<0.05),0.1mol/Ltrehalose和5.0mmol/LEDTA混合处理组的精子生存率最高。0.1mol/Ltrehalose和2~5mmol/LEDTA混合处理组精子顶体完整率显著高于trehalose处理组(P<0.05),0.05~0.1mol/Ltrehalose和5.0mmol/LEDTA混合处理组低渗膨胀精子率显著高于trehalose处理组(P<0.05)。trehalose和EDTA混合处理组精子内活性氧(ROS)含量总体上高于trehalose处理组(P<0.05)。这些结果显示稀释剂中trehalose和EDTA增补能改善精液特性,但不能抑制精子内ROS的发生。 相似文献
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The effects of resveratrol on the quality of frozen-thawed sheep semen were studied in this study.Semen was collected from six Yunnan semi-fine wool sheep using artificial vagina.The semen was diluted with the Optidyl extender supplemented with resveratrol with a concentration of 0,0.1,1,10,or 20 μmol/L,followed by loading into plastic straws and equilibration at a low temperature.Then,the straws were pre-frozen in liquid nitrogen vapor,followed by storage in liquid nitrogen for 30 days.After thawing for 30 seconds in a 37 ℃ water bath,the parameters including sperm motility,acrosome integrity,membrane integrity,distribution of phosphatidylserine (PS) and ROS were measured.The results showed that the post-thaw sperm total motility,progressive motility and the rate of sperm with curved tail were 76.14%±0.97%,43.56%±0.91% and 43.24%±1.68% in the 10 μmol/L resveratrol group,respectively,which were significantly higher than those in the other groups (P<0.05).However,when the concentration of resveratrol in the freezing extender was 20 μmol/L,the post-thaw total motility,progressive motility and the rate of sperm with curved tail were 21.78%±0.79%,25.23%±1.34% and 4.84%±0.68%,respectively,which were significantly lower than those in the other groups (P<0.05).The acrosome integrity of sperm frozen in the 10 μmol/L resveratrol group was best,which was 50.47%±0.91% and significantly higher than the other treatments (P<0.05).The results of PS distribution showed that the percentage of viable sperm in the 10 μmol/L resveratrol group was 46.43%±2.95%,and significantly higher than that in the 20 μmol/L group (31.14%±3.56%,P<0.05),but there was no significant difference with the other groups (P>0.05).In addition,the PS labeling rate of sperm in the 20 μmol/L resveratrol group (39.82%±3.38%) was significantly higher than those of the other groups (P<0.05).In terms of ROS production,this study demonstrated that the rate of viable sperm (ROS and PI were negative) in the 10 μmol/L resveratrol group (63.57%±0.71%) was significantly higher than that of the other groups (P<0.05),while the rate of viable sperm (32.45%±1.42%) in the 20 μmol/L resveratrol group was significantly lower than those of the other groups (P<0.05).In conclusion,the post-thaw quality of sheep semen could be improved after the addition of resveratrol to the freezing extenders,which might be related to the antioxidant properties of resveratrol.But,the cryoprotective effects of resveratrol dependents on its used doses,and the optimal concentration was 10 μmol/L based on this present study.However,excessively high concentration of resveratrol could aggravate cryodamage on sperm.In addition,the protective effects of resveratrol on sheep sperm still needs to be verified by in vitro fertilization and artificial insemination. 相似文献
16.
试验旨在研究白藜芦醇对绵羊冷冻精液质量的改善效果。采用假阴道法采集6只云南半细毛羊精液，用含不同浓度（0、0.1、1、10、20 μmol/L）白藜芦醇的Optidyl稀释液稀释后进行细管分装，低温平衡和液氮气相预冻后，在液氮中保存30 d。解冻后测定精子活力、质膜完整性、磷脂酰丝氨酸（PS）分布、顶体完整性和活性氧等指标。结果表明，解冻后10 μmol/L白藜芦醇组精子总活力、直线运动百分率、精子弯尾率分别为76.14%±0.97%、43.56%±0.91%、43.24%±1.68%，均显著高于其他各组（P<0.05）；而20 μmol/L白藜芦醇组精子总活力、直线运动百分率、精子弯尾率分别为21.78%±0.79%、25.23%±1.34%、4.84%±0.68%，均显著低于其他各组（P<0.05）。10 μmol/L白藜芦醇组精子顶体完整性最高，为50.47%±0.91%，显著高于其他各处理组（P<0.05）。PS分布结果表明，10 μmol/L白藜芦醇组正常精子百分率为46.43%±2.95%，显著高于20 μmol/L组（31.14%±3.56%，P<0.05），与其他各组无显著性差异（P>0.05）。20 μmol/L白藜芦醇组PS标记率（39.82%±3.38%）显著高于其他处理组（P<0.05）。活性氧试验结果表明，10 μmol/L白藜芦醇组正常精子（63.57%±0.71%）显著高于其他各组（P<0.05）；而20 μmol/L白藜芦醇组正常精子（32.45%±1.42%）显著低于其他各组（P<0.05）。综上，在冷冻稀释液中添加白藜芦醇可以改善绵羊冷冻精液品质，这与白藜芦醇的抗氧化特性有关。但是，白藜芦醇的冷冻保护效果具有明显的浓度依赖性，其最佳作用浓度为10 μmol/L，过高浓度的白藜芦醇反而加重精子的冷冻损伤。此外，对于白藜芦醇对绵羊精子的抗冻保护效果仍然需要体外受精或人工授精验证。 相似文献
17.
谷胱甘肽对犬精液冷冻保存效果的影响 《畜牧与饲料科学》2019,40(11):65-69
为探讨在冷冻稀释液中添加谷胱甘肽（GSH）对犬精液冷冻保存效果的影响,采用按摩法采集5只杂种土犬的精液,离心去精清后,在冷冻液中分别加入0.5、1.0、1.5、2.0、2.5 mmol/L的GSH,制成0.25 mL的冻精进行冷冻保存,以不添加GSH的处理组作为对照组。解冻后在含有5% CO2的空气、37 ℃、相对饱和湿度条件下孵育10 h,分别在孵育0、2、4、6、8、10 h时检查精子活力。结果显示：冻融后0 h,0.5、1.0 mmol/L GSH处理组的精子活力较高,分别为0.36和0.38,均显著（P<0.05）高于对照组和2.0、2.5 mmol/L处理组,且两者之间差异不显著（P>0.05）;1.0 mmol/L处理组的精子顶体完整率最高,为85.10%,显著（P<0.05）高于对照组,同时,其精子畸形率最低,为23.00%,显著（P<0.05）低于对照组。冻融后体外孵育2、4 h时,0.5、1.0 mmol/L处理组的精子活力均较高,其中,0.5 mmol/L处理组在体外孵育4 h时,其精子活力仍可达到0.30;孵育6 h时,1.0 mmol/L处理组精子活力最高,显著（P<0.05）高于对照组和2.0、2.5 mmol/L处理组;孵育8 h时,各GSH处理组的精子活力均显著（P<0.05）高于对照组;在孵育至10 h时,各GSH处理组的精子活力较其他孵育时间均有较大幅度的下降,未检测到对照组中有呈直线运动的精子。综上提示,在犬精液冷冻液中添加0.5~1.0 mmol/L的GSH能够显著提高冻融后的精子质量和体外存活时间。 相似文献
18.
In this study, two following experiments were performed to improve post-thaw motility and viability of frozen-thawed ram spermatozoa. We examined i) the effects of different concentrations of bovine serum albumin (0, 0.3, 1, 5, 10 and 15% BSA) in semen diluents lacking egg yolk and ii) the effects of four semen diluents, fructose (F: control) and trehalose (T) in semen diluents containing egg yolk, 15% BSA in semen diluents without egg yolk (BSA), and modified phosphate buffered saline (m-PBS). Frozen-thawed spermatozoa were examined for progressive sperm motility, viability, morphological abnormality, sperm tail swelling test, and sperm acrosome integrity. In Experiment 1, the rates of sperm motility immediately after thawing (0 h) were significantly (P<0.05) higher in the 10 and 15% BSA groups (55.0 +/- 2.9 and 58.3 +/- 6.7%, respectively) than in the positive control (F) group (41.7 +/- 4.4%). The rate of sperm viability in the negative control (0% BSA) group (80.2 +/- 3.3%) was significantly (P<0.05) lower than in the positive control (F) group (89.8 +/- 1.5%), but when compared with the F group, no significant differences were found among the 0.3, 1, 5, 10 and 15% BSA groups at 0 h. The rates of sperm morphological abnormality of the 10 and 15% BSA groups (6.5 +/- 1.3 and 6.3 +/- 1.1%, respectively) were significantly (P<0.05) lower at 0 h than that in the 1% BSA group (16.3 +/- 5.2%). In Experiment 2, T addition improved (P<0.05) the post-thaw motility compared with the F and BSA groups. Furthermore, at 3 and 6 h, the post-thaw motility of the T group (36.3 +/- 2.4 and 25.0 +/- 2.0%, respectively) was significantly (P<0.05) higher than in the BSA (26.3 +/- 2.4 and 18.8 +/- 1.3%, respectively) and F (28.8 +/- 3.8 and 18.8 +/- 2.4%, respectively) groups. The post-thaw sperm motility and viability in the m-PBS group were significantly (P<0.05) lower than those of the control (F), T, and BSA groups throughout all observation points. These results indicate that 10 and 15% BSA can be substituted for egg-yolk for ram semen diluent and that the addition of trehalose enhances motility and viability of ram spermatozoa after freezing and thawing. 相似文献
19.
采用5% 二甲乙酰胺(DMA)(V/V)完全替代甘油,比较乳糖、海藻糖对精液冷冻保存效果的影响。结果表明,海藻糖显著提高了冷冻——解冻后精子成活力(49.32%±1.52%)与顶体完整性 (47.33%±1.16%)(P<0.05)。然后利用海藻糖替代乳糖,评价不同浓度的DMA对公猪精液冷冻保存的影响。结果表明,当DMA添加量为4%时,解冻后精子活率、成活力、顶体完整率分别为(45.17±0.56)%、(50.33±0.67)%、(48.30±1.44)%,均显著高于3% DMA、6% DMA添加组(P<0.05),精子活率显著高于5% DMA添加组(P<0.05),但精子成活力、顶体完整性与其差异不显著(P>0.05)。因此,当利用海藻糖作为冷冻保存基础稀释液,DMA最适添加量为4%。 相似文献