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1.
以纯化的禽流感病毒（avian influenza virus,AIV）核蛋白作为捕捉抗原，金标兔抗鸡抗体作为金标二抗，建立检测禽流感血清抗体的胶体金免疫层析试纸条（immunochromatographic strip, ICS）。对标准阳性血清不同滴度进行检测，ICS试验的敏感性高于琼脂扩散试验（AGP）。特异性试验证实，禽流感的ICS试验与新城疫（ND）、传染性支气管炎（IBV）、鸡传染性法氏囊病（IBD）及减蛋综合症（EDS-76）均无交叉反应。同时，用ICS试验和HI试验检测105份鸡血清，两者具有较好的符合性。结果认为：禽流感ICS具有快速简便、特异、灵敏等优点。 相似文献
2.
鸡肾型传染性气管炎病毒分离及其血凝特性 总被引：4，自引：0，他引：4
从４个不同的发病鸡场分离出４株肾传支病毒，命名为Ｘ、Ｂ１、Ｂ２、Ｂ２株，并成功地感染鸡，复制出肾传支病例。选Ｂ２株与以前本室保存的肾传支病毒Ａ株经鸡胚培养、浓缩、胰酶处理制成ＨＡ抗原，对该抗原的稳定性与肾传支阴性血清，新城疫阳性血清、肾传支高免血清进行ＨＩ试验。结果表明，由Ｂ２毒株制备的ＨＡ抗原稳定性很好，新城疫阴性血清和肾传支阴性血清均不能抑制其血凝活性，肾传支高兔血清能充分地抑制其血凝活性。 相似文献
3.
通过不同的免疫途径对商品蛋雏鸡进行免疫,探讨不同的免疫途径对商品蛋雏鸡新城疫(Newcastle disease,ND)黏膜抗体及血清抗体的影响。结果表明,同时用新城疫弱毒疫苗免疫后,不同的免疫途径产生的新城疫黏膜抗体及血清抗体效价有较大差异。采用喷雾法于3日龄首免,15日龄二免,25日龄三免,鸡体产生保护水平(6 log2)以上的ND黏膜抗体和血清抗体最快、抗体水平最高、维持时间最长、总体免疫效果最好,而采用饮水免疫的试验组产生的ND黏膜抗体和血清抗体水平均不高,总体免疫效果最差。 相似文献
4.
利用新城疫Clone30株病毒研制了检测新城疫抗体的ELISA试剂盒。抗原最佳包被浓度为1．086μg／ml，被检血清最佳稀释度为1：100，酶标结合物最佳工作浓度1：800，血清样品阴阳性临界值为0．34。本试剂盒和HIPRA的ND试剂盒检测64份血清相比较，结果表明本试剂盒的特异性和敏感性分别为90％和93．2％。试剂盒保存期试验结果表明，试剂盒可保存3个月。 相似文献
5.
新城疫快速诊断试纸条的研制及初步应用 总被引：5，自引：1，他引：4
以红色胶体金标记鸡新城疫(ND)抗体，兔抗鸡ND抗体和兔抗鸡二抗包被于硝酸纤维素膜上，研制出快速诊断鸡新城疫的免疫层析测试条。将试纸条插入样品中，利用滤膜的层析作用，使加在膜一端的样品，向另一端移动，在移动过程中抗原与抗体发生反应，产生肉眼可见的红色条带。该法具有操作简便、快速准确、特异性强、重复性好，无需任何仪器设备等特点，对于鸡场中快速诊断及防治本病具有重大意义。 相似文献
6.
对40~45日龄鸽群实施新城疫低等毒力活疫苗（La系）免疫与新城疫低等毒力活疫苗（La系）加H5N2亚型禽流感灭活疫苗免疫效果的对比试验，首免后21 d进行二次免疫，并采用血凝抑制试验对80份血清进行ND和AIV H5血清抗体跟踪检测。结果表明，使用新城疫低等毒力活疫苗（La系）免疫的鸽群首免后7~21 d，ND免疫抗体随着日龄的增加而升高，但不能达到有效保护水平；首免后21 d进行二次免疫，跟踪监测到二免后35 d，ND免疫抗体呈波浪式上升；使用新城疫低等毒力活疫苗（La系）加H5N2亚型禽流感灭活疫苗免疫的鸽群抗体水平总体趋势基本相同，但ND免疫抗体水平波动较大，是否与同时免疫AIV H5有关则需要进一步试验探讨。 相似文献
7.
ELISA检测鸡新城疫病毒特异性IgM抗体的研究 总被引：3，自引：0，他引：3
以新城疫病毒单克隆抗体包被板,用10％小牛血清-PBS封闭后,捕获尿囊液中的新城疫病毒作固相抗原.在此板上应用酶标抗鸡IgM单克隆抗体进行间接ELISA试验检侧鸡血清中新城疫病毒的特异性IgM抗体.试验证明该方法特异性强、敏惑性高.兔抗新城疫病毒阳性血清可特异性阻断反应,将新城疫病IgM阳性血清用2-ME处理可使ELISA反应呈阴性,与禽源多杀性巴氏杆菌鸡IgM阳性血清、鸡传染性支气管炎病毒IgM阳性血清无交叉反应.该试验可检测到La Sota免疫后3天鸡血清中的特异性IgM,对鸡新城疫病毒IgM阳性血清的检测效价可达1:320以上,并可检测到临床新城疫病鸡血清中的特异性IgM抗体. 相似文献
8.
应用琼脂扩散试验检测新城疫抗原 总被引：6，自引：0，他引：6
应用ＰＥＧ平板进行琼脂扩散试验检测新城疫抗原,提高了对新城疫抗原的检出率。采用多器官统一判定方法,即脾、胸腺、盲肠扁桃体,肠淋巴结和法氏囊等器官检出阳性时即可判为新城疫,对新城疫死鸡的检出率为１００％,解决非典型新城疫诊断困难这一难题,方法简单准确,快速易行,值得推广和应用。 相似文献
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10.
肉鸡ND,IBD,IB弱毒疫苗联合气雾免疫试验 总被引：1，自引：0，他引：1
一、材料与方法1.疫苗:试验所用的疫苗均为杭州药厂兽药分厂生产。批号:ND(Lasota苗)890623—1,ND(Ⅰ系)890403—2,ND(Ⅱ系)890523—1。IBD891227—2,IB_(H120)891202—1,IB_(H52)891110—3。2.抗原、阳性血清:ND抗原及阳性血清由省农科院提供(批号为8905);IBD抗 相似文献
11.
应用ＰＥＧ平板进行琼脂扩散试验检测新城疫抗原,提高了对新城疫抗原的检出率。采用多器官检查和统一判定方法,即脾、胸腺、盲肠扁桃体、肠淋巴结和法氏囊等器官检出阳性时即可判为新城疫,对新城疫死鸡的检出率为１００％,解决了非典型新城疫诊断困难这一难题,方法简单准确,快速易行,值得推广和应用。 相似文献
12.
鸡新城疫病毒抗原超滤技术研究 总被引：3，自引：1，他引：2
应用分子筛过滤技术超滤浓缩鸡新城疫病毒,并就ＮＤＶ抗原量对鸡免疫应答的和了动态研究,试验结果显示,将ＮＤＶ对外开放尿囊毒浓缩４倍体积,ＨＡ效价提高了２个滴度,病毒回收率达１００％;病毒活性亦未发生改变。免疫１１天后,ＮＤ浓缩苗和ＮＤ－ＩＢＤ二联苗组鸡的ＮＤ－ＨＩ抗体水平远远高于免疫对照组,差异极显著。结果表明,所建超滤技术适用于ＮＤＶ怕的浓缩应用;适量增另ＮＤＶ怕量能够加快鸡的体液免疫应答,增强鸡 相似文献
13.
鸡新城疫和传染性法氏囊病灭活疫苗抗原量对鸡细胞免疫的影响 总被引：2，自引：0，他引：2
将不同抗原含量的鸡新城疫和传染性法氏囊病油乳剂灭活疫苗分别免疫１７日龄雏鸡，测定了疫苗抗原含量与细胞免疫应答之间的关系。经Ｔ淋巴细胞转化试验结果表明，两种疫苗的抗原含量－细胞免疫应答曲线是一致的，随着抗原量的增加，活化的Ｔ淋巴细胞数量也相应增多，但是细胞免疫应答的可测时间是暂时的，于免疫７天达高峰，１７天后下降接近正常水平。 相似文献
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15.
Knowledge of the dose-response relation of inactivated vaccines and of the factors that influence this relation is essential for the evaluation of existing vaccine potency assays and the development of new potency assays that are based on the antigen content of the inactivated vaccines. We quantified the relation between vaccine dose, serologic response, and clinical protection after vaccination for three different inactivated Newcastle disease (ND) vaccines. Qualitatively, similar dose-response curves were obtained for the three vaccines when either the serologic response or the clinical protection of specific-pathogen-free (SPF) chickens was plotted against the different vaccine doses applied. However, the vaccines differed quantitatively: doses of vaccines that induced similar antibody titers or clinical protection differed 2-8-fold. In contrast with the narrow range of antibody titers induced by a full vaccine dose, a very broad range of titers was obtained after dilution of the vaccines. At least 95% of the SPF chickens with detectable antibody in the serum were protected against a challenge with virulent Herts ND virus. The relation between the dosage of two different ND vaccines and the serum antibody titers remained markedly constant between 3 and 18 wk after vaccination. Vaccination of broilers instead of layers with a dilution series of inactivated ND vaccine resulted in significantly lower antibody levels and less clinical protection against virulent challenge. In conclusion, despite quantitative differences, we found comparable dose-response relations for the three inactivated ND vaccines studied. 相似文献
16.
Monoclonal antibodies (mAb) were produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c and ND4 mice that were immunized with killed Leptospira interrogans serovar pomona whole cells. Thirty hybridomas which produced antibodies (of the IgG1, IgG2a, IgG2b, or IgG3 isotype) that bound to epitopes on the serovar pomona whole cell antigen were identified by an indirect enzyme-linked immunosorbent assay (ELISA). Twenty-eight of these 30 mAbs cross-reacted in the indirect ELISA with at least one whole cell antigen prepared from 12 other pathogenic Leptospira serovars, and/or with whole cell antigen from the non-pathogenic Leptospira biflexa serovar patoc. The two serovar pomona-specific mAbs, which were designated M897 and M898, were obtained from the ND4 mouse and were both of the IgG1 isotype. In competitive ELISAs, M897 and M898 were inhibited from binding to the pomona antigen by bovine sera with anti-serovar pomona microscopic agglutination test (MAT) titres ranging from 100 to 6400. No significant inhibition was observed with pomona MAT-negative sera or with sera from animals experimentally infected with serovars canicola, copenhageni, grippotyphosa, hardjo type hardjobovis or sejroe. The epitopes recognized by M897 and M898 were both highly susceptible to sodium meta-periodate oxidation, indicating a carbohydrate composition. Neither of these mAbs reacted in immunoblots with the separated components of the serovar pomona whole cell antigen. 相似文献
17.
Bwala DG Clift S Duncan NM Bisschop SP Oludayo FF 《Research in veterinary science》2012,93(1):520-528
The control of Newcastle disease (ND) in South Africa has proved difficult since 2002 following the introduction of lineage 5d/VIId Newcastle disease virus (NDV) strain ("goose paramyxovirus" - GPMV) to which commercially available ND vaccines appeared less effective. Most of the ND infections, even in fully vaccinated hens were characterized consistently by a drop in egg production. In this study, commercial and SPF hens-in-lay were vaccinated with La Sota vaccine and challenged with a GPMV isolate. Immunohistochemical labeling was used to determine the distribution of viral antigen in the oviduct of the hens. Following reports that cloacal vaccination offered better protection against egg production losses than the oro-nasal route, the efficacy of cloacal and ocular routes of vaccination against challenge were compared. Results showed that La Sota vaccine offered birds 100% protection against the virulent ND (GPMV) virus challenge from clinical disease and death, but not against infection and replication of the GPMV, as birds showed varying degrees of macropathology. Histopathology of the oviduct of infected birds revealed multifocal lymphocytic inflammation in the interstitium as well as mild glandular ectasia and mild edema. Finely granular NDV-specific immunolabeling was demonstrated in the cytoplasm of epithelial cells and mononuclear (lymphohistiocytic) cells in the interstitium of the oviduct. Both vaccine and virulent GPMV showed greatest tropism for the uterus (versus the magnum and isthmus). There was no clear difference in the protection of the oviduct and in the distribution of oviductal GPMV antigens between the two routes of vaccination. 相似文献
18.
Spector D Legendre AM Wheat J Bemis D Rohrbach B Taboada J Durkin M 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2008,22(4):839-843
BACKGROUND: Early diagnosis and treatment are associated with an improved prognosis in blastomycosis. The diagnosis of blastomycosis may be missed by cytology, histopathology, culture, or serology. An enzyme immunoassay (EIA) for detection of Blastomyces dermatitidis galactomannan antigen in body fluids has been used for rapid diagnosis of blastomycosis in humans. HYPOTHESIS: Measurement of Blastomyces antigen in urine or serum by the MVista Blastomyces antigen EIA is more sensitive than measurement of anti-Blastomyces antibodies for diagnosis of blastomycosis in dogs. METHODS: Serum and urine samples from 46 dogs with confirmed blastomycosis were tested for Blastomyces antigen and serum was tested for anti-Blastomyces antibodies. RESULTS: The sensitivity for the detection of antigen in urine was 93.5% and it was 87.0% in serum. The sensitivity of antibody detection by agar gel immunodiffusion (AGID) was 17.4% and it was 76.1% by EIA. Antigen and antibody decreased during itraconazole treatment. CONCLUSIONS AND CLINICAL IMPORTANCE: Antigen detection is a more sensitive test for diagnosis of blastomycosis than antibody testing by AGID, the only commercially available method. Antigen concentrations decreased with treatment. 相似文献
19.
Newcastle disease (ND) is a highly contagious disease of chickens causing significant economic losses worldwide. Due to the limitation in their efficacy, current vaccination strategies against ND need improvements. This study aimed to evaluate a new-generation ND vaccine for its efficacy in providing clinical protection and reducing virus shedding after challenge. Broiler chickens were vaccinated in ovo or subcutaneously at hatch with a turkey herpesvirus-based recombinant vaccine (rHVT) expressing a key protective antigen (F glycoprotein) of Newcastle disease virus (NDV). Groups of birds were challenged at 20, 27, and 40 days of age with a genotype V viscerotropic velogenic NDV strain. Protection was 57% and 81%, 100% and 95%, and 100% and 100% after the subsequent challenges in the in ovo and subcutaneously vaccinated chickens, respectively. Humoral immune response to vaccination could be detected from 3-4 wk of age. Challenge virus shedding was lower and gradually decreased over time in the vaccinated birds compared to the unvaccinated control chickens. In spite of the phylogenetic distance between the NDV F gene inserted into the vector vaccine and the challenge virus (genotype I and V, respectively), the rHVT NDV vaccine provided good clinical protection and significantly reduced challenge virus shedding. 相似文献